Abstract:
:Recombinant human erythropoietin (rHuEPO) is a biopharmaceutical drug given to patients who have a low hemoglobin related to chronic kidney disease, cancer or anemia. However, some patients repeatedly receiving rHuEPO develop anti-rHuEPO neutralizing antibodies leading to the development of pure red cell aplasia (PRCA). The immunogenic antibody response activated by rHuEPO is believed to be triggered by T-cells recognizing EPO epitopes bound to MHC molecules displayed on the cell surface of APCs. Previous studies have reported an association between the development of anti-rHuEpo-associated PRCA and the HLA-DRB1*09 gene, which is reported to be entrenched in the Thai population. In this study, we used computational design to screen for immunogenic hotspots recognized by HLA-DRB1*09, and predicted seventeen mutants having anywhere between one through four mutations that reduce affinity for the allele, without disrupting the structural integrity and bioactivity. Five out of seventeen mutants were less immunogenic in vitro while retaining similar or slightly reduced bioactivity than rHuEPO. These engineered proteins could be the potential candidates to treat patients who are rHuEpo-dependent and express the HLA-DRB1*09 allele.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Susantad T,Fuangthong M,Tharakaraman K,Tit-Oon P,Ruchirawat M,Sasisekharan Rdoi
10.1038/s41598-020-80402-1subject
Has Abstractpub_date
2021-01-15 00:00:00pages
1491issue
1issn
2045-2322pii
10.1038/s41598-020-80402-1journal_volume
11pub_type
杂志文章abstract::Understanding the molecular mechanism by which epithelial mesenchymal transition (EMT)-mediated cancer metastasis and how microRNA (miRNA) regulates lung cancer progression via Twist1-activated EMT may provide potential therapeutic targets for cancer therapy. Here we found that miR-33a, an intronic miRNA located withi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13677
更新日期:2015-09-02 00:00:00
abstract::High-pressure Raman spectroscopy and x-ray diffraction of Sb2S3 up to 53 GPa reveals two phase transitions at 5 GPa and 15 GPa. The first transition is evidenced by noticeable compressibility changes in distinct Raman-active modes, in the lattice parameter axial ratios, the unit cell volume, as well as in specific int...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24246
更新日期:2016-04-06 00:00:00
abstract::N-doped carbon nanosheets/vanadium nitride nanoparticles (N-CNS/VNNPs) are synthesized via a novel method combining surface-initiated in-situ intercalative polymerization and thermal-treatment process in NH3/N2 atmosphere. The pH value of the synthesis system plays a critical role in constructing the structure and enh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21082-w
更新日期:2018-02-13 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-32881-6
更新日期:2018-10-12 00:00:00
abstract::Several mosquito species have been described as vectors for the Zika virus (ZIKV), such as those in the Aedes, Anopheles, Mansonia and Culex genera. Our previous survey studies were found the ZIKV RNA positive in both male, female and larvae of Culex quinquefasciatus Say and Aedes aegypti (L.) mosquitoes collected fro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41727-8
更新日期:2019-03-27 00:00:00
abstract::Friction is a major inhibitor in almost every mechanical system. Enlightened by the Leidenfrost effect - a droplet can be levitated by its own vapor layer on a sufficiently hot surface - we demonstrate for the first time that a small cart can also be levitated by Leidenfrost vapor. The levitated cart can carry certain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00797
更新日期:2012-01-01 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-42462-w
更新日期:2019-04-17 00:00:00
abstract::Neural networks can efficiently encode the probability distribution of errors in an error correcting code. Moreover, these distributions can be conditioned on the syndromes of the corresponding errors. This paves a path forward for a decoder that employs a neural network to calculate the conditional distribution, then...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11266-1
更新日期:2017-09-08 00:00:00
abstract::In order to study the molecular differences between type 2 diabetes mellitus (T2DM) and T2DM with depression (DD), we aimed to screen the differential expression of lncRNA, mRNA, and circRNA in the blood of patients with T2DM and DD. Based on the self-rating depression scale (SDS), patient health questionnaire 9 (PHQ9...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47077-9
更新日期:2019-07-24 00:00:00
abstract::The mammalian Major Histocompatibility Complex (MHC) region contains several gene families characterized by highly polymorphic loci with extensive nucleotide diversity, copy number variation of paralogous genes, and long repetitive sequences. This structural complexity has made it difficult to construct a reliable ref...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45518
更新日期:2017-03-31 00:00:00
abstract::Differences in the pathogenesis and clinical characteristics between lamellar macular hole (LMH) with and without LMH-associated epiretinal proliferation (LHEP) can have surgical implications. This study investigated the effects of treating LHEP by foveolar internal limiting membrane (ILM) non-peeling and epiretinal p...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-52447-4
更新日期:2019-11-05 00:00:00
abstract::Soil water retention curve (SWRC) plays an important role in simulating soil water movement and assessing soil water holding capacity and availability. Comparison of fitness between different models to determine the best SWRC model of specific regions is required. In this study, three popular models, van Genuchten, Br...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54449-8
更新日期:2019-12-05 00:00:00
abstract::Mind wandering (MW) refers to the disengagement of attention from the external environment and the generation of thoughts unrelated to the task at hand. It is a ubiquitous cognitive process resulting in lapses of attention. MW imposes a negative impact on attention-based task performance, but also has been associated ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10616-3
更新日期:2017-08-31 00:00:00
abstract::Seed number per pod (SNPP) is one of the major yield components and breeding targets in rapeseed that shows great variation and is invaluable for genetic improvement. To elucidate the genetic architecture and uncover the mechanism of SNPP, we identified five quantitative trait loci (QTLs) using the BnaZNRIL population...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24124
更新日期:2016-04-12 00:00:00
abstract::Adherence to norms and interventions to norm violations are two important forms of social behaviour modelled in economic games. While both appear to serve a prosocial function, they may represent separate mechanisms corresponding with distinct emotional and psychological antecedents, and thus may be predicted by diffe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02952-1
更新日期:2017-06-13 00:00:00
abstract::The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) to reach their full potential in the treatment of chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel was developed to deliver BMSCs, which improve the chronic inflammation microenvironments ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18104
更新日期:2015-12-08 00:00:00
abstract::Relying on an exact time evolution scheme, we identify a novel transient energy transfer phenomenon in an exactly-solvable quantum microscopic model consisting of a three-level system coupled to two non-Markovian zero-temperature bosonic baths through two separable quantum channels. The dynamics of this model can be s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15332
更新日期:2015-10-19 00:00:00
abstract::The interaction of osseous tissue with electric fields is an important subject. The electrical stimulation of bone promotes osteogenesis, while bone impedance has been proposed as a measure of osteoporosis, to follow fracture healing, or as a method to improve safety of surgical procedures. However, a deeper understan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26836-0
更新日期:2018-06-05 00:00:00
abstract::The bacterial second messenger cyclic di-3',5'-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs) have been identified as key targets to aid development of novel ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42166
更新日期:2017-02-10 00:00:00
abstract::There are at least two different principles of how ADP-ribose (ADPR) induces activation of TRPM2 channels. In human TRPM2, gating requires the C-terminal NUDT9H domain as ADPR-binding module, whereas in sea anemone, NUDT9H is dispensable and binding of ADPR occurs N-terminally. Zebrafish TRPM2 needs both, the N-termin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55232-5
更新日期:2019-12-16 00:00:00
abstract::Prospective observation of hemodynamic changes before and after the formation of atherosclerotic stenosis in the carotid artery is difficult. Thus, a vessel surface repairing method was used for retrospective hemodynamic study before and after atherosclerotic stenosis formation in carotid artery. The three-dimensional...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23842-0
更新日期:2018-04-03 00:00:00
abstract::All species face the important adaptive problem of efficiently locating high-quality nutritional resources. We explored whether human spatial cognition is enhanced for high-calorie foods, in a large multisensory experiment that covertly tested the location memory of people who navigated a maze-like food setting. We fo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72570-x
更新日期:2020-10-08 00:00:00
abstract::We report, for the first time, that the energy of femtosecond optical laser pulses, E, with relativistic intensities I > 10(21) W/cm(2) is efficiently converted to X-ray radiation, which is emitted by "hot" electron component in collision-less processes and heats the solid density plasma periphery. As shown by direct...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13436
更新日期:2015-09-02 00:00:00
abstract::Recent studies have indicated that different post-translational modifications (PTMs) synergistically orchestrate specific biological processes by crosstalks. However, the preference of the crosstalk among different PTMs and the evolutionary constraint on the PTM crosstalk need further dissections. In this study, the i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07331
更新日期:2014-12-05 00:00:00
abstract::We report the high susceptibility of several clinical isolates of Propionibacterium acnes from different sources (skin, bone, wound exudates, abscess or blood contamination) to the head-to-tail cyclized bacteriocin AS-48. This peptide is a feasible candidate for further pharmacological development against this bacteri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29580-7
更新日期:2018-08-06 00:00:00
abstract::We characterised wool traits, and skin gene expression profiles of fine wool Super Merino (SM) and coarse wool Small Tail Han (STH) sheep. SM sheep had a significantly higher total density of wool follicles, heavier fleeces, finer fibre diameter, and increased crimp frequency, staple length and wool grease (lanolin) p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14772-4
更新日期:2017-10-30 00:00:00
abstract::Aspergillus flavus is a pathogenic fungus infecting maize and producing aflatoxins that are health hazards to humans and animals. Characterizing host defense mechanism and prioritizing candidate resistance genes are important to the development of resistant maize germplasm. We investigated methods amenable for the ana...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04815
更新日期:2014-04-28 00:00:00
abstract::Sea ice is an important component of the global climate system and a key indicator of climate change. A decreasing trend in Arctic sea-ice concentration is evident in recent years, whereas Antarctic sea-ice concentration exhibits a generally increasing trend. Various studies have investigated the underlying causes of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45804
更新日期:2017-04-05 00:00:00
abstract::Blood coagulation is regulated through protein-protein and protein-lipid interactions that occur at the sub-endothelium following vascular damage. Soluble clotting proteins bind to membrane components in a phosphatidylserine (PS) dependent manner to assemble multi-protein complexes that regulate clot formation; howeve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73647-3
更新日期:2020-10-15 00:00:00
abstract::There has been a rapid increase in Danish pig herds testing positive for livestock-associated Methicillin-resistant Staphylococcus aureus (LA-MRSA) since the first screening in 2008. Despite a national action plan to control LA-MRSA in the Danish pig population, 88% of pig herds tested positive in a 2016 cross-section...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37075-8
更新日期:2019-01-24 00:00:00