A novel RNA molecular signature for activation of 2'-5' oligoadenylate synthetase-1.


:Human 2'-5' oligoadenylate synthetase-1 (OAS1) is central in innate immune system detection of cytoplasmic double-stranded RNA (dsRNA) and promotion of host antiviral responses. However, the molecular signatures that promote OAS1 activation are currently poorly defined. We show that the 3'-end polyuridine sequence of viral and cellular RNA polymerase III non-coding transcripts is critical for their optimal activation of OAS1. Potentiation of OAS1 activity was also observed with a model dsRNA duplex containing an OAS1 activation consensus sequence. We determined that the effect is attributable to a single appended 3'-end residue, is dependent upon its single-stranded nature with strong preference for pyrimidine residues and is mediated by a highly conserved OAS1 residue adjacent to the dsRNA binding surface. These findings represent discovery of a novel signature for OAS1 activation, the 3'-single-stranded pyrimidine (3'-ssPy) motif, with potential functional implications for OAS1 activity in its antiviral and other anti-proliferative roles.


Nucleic Acids Res


Nucleic acids research


Vachon VK,Calderon BM,Conn GL




Has Abstract


2015-01-01 00:00:00














  • DynaMut: predicting the impact of mutations on protein conformation, flexibility and stability.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Rodrigues CH,Pires DE,Ascher DB

    更新日期:2018-07-02 00:00:00

  • Carbon nanotubes selective destabilization of duplex and triplex DNA and inducing B-A transition in solution.

    abstract::Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nanodevice applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mecha...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Li X,Peng Y,Qu X

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  • Regulation of the Oct-4 gene by nuclear receptors.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sylvester I,Schöler HR

    更新日期:1994-03-25 00:00:00

  • GeConT 2: gene context analysis for orthologous proteins, conserved domains and metabolic pathways.

    abstract::The Gene Context Tool (GeConT) allows users to visualize the genomic context of a gene or a group of genes and their orthologous relationships within fully sequenced bacterial genomes. The new version of the server incorporates information from the COG, Pfam and KEGG databases, allowing users to have an integrated gra...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Martinez-Guerrero CE,Ciria R,Abreu-Goodger C,Moreno-Hagelsieb G,Merino E

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  • NF-kappaB controls the global pro-inflammatory response in endothelial cells: evidence for the regulation of a pro-atherogenic program.

    abstract::Activation of the transcription factor NF-kappaB is critical for the tumor necrosis factor-alpha (TNF-alpha)-induced inflammatory response. Here we report the complete gene expression profile from activated microvascular endothelial cells emphasizing the direct contribution of the NF-kappaB pathway. Human microvascula...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kempe S,Kestler H,Lasar A,Wirth T

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Pradhan S,Talbot D,Sha M,Benner J,Hornstra L,Li E,Jaenisch R,Roberts RJ

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  • The interaction of DNA-targeted platinum phenanthridinium complexes with DNA.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Whittaker J,McFadyen WD,Wickham G,Wakelin LP,Murray V

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ritchie HH,Wang LH,Tsai S,O'Malley BW,Tsai MJ

    更新日期:1990-12-11 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lemp NA,Hiraoka K,Kasahara N,Logg CR

    更新日期:2012-08-01 00:00:00

  • alpha-DNA. VII. Solid phase synthesis of alpha-anomeric oligodeoxyribonucleotides.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Morvan F,Rayner B,Leonetti JP,Imbach JL

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  • Is there specific transcription from isolated chromatin?

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Konkel DA,Ingram VM

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    journal_title:Nucleic acids research

    pub_type: 杂志文章,评审


    authors: Prescott DM

    更新日期:1999-03-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tsukahara T,Casciato C,Helfman DM

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gambaryan AS,Venkstern TV,Bayev AA

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Mruk I,Blumenthal RM

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    journal_title:Nucleic acids research

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    authors: Moreau P,Hen R,Wasylyk B,Everett R,Gaub MP,Chambon P

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    journal_title:Nucleic acids research

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    authors: Otto B,Fanning E

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    journal_title:Nucleic acids research

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    journal_title:Nucleic acids research

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    authors: Chen XW,Jeong JC,Dermyer P

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    journal_title:Nucleic acids research

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    authors: Colantuoni V,Romano V,Bensi G,Santoro C,Costanzo F,Raugei G,Cortese R

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    journal_title:Nucleic acids research

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    authors: Fuchs E,Falschlunger C,Micura R,Breuker K

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    journal_title:Nucleic acids research

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    authors: Wiest NE,Houghtaling S,Sanchez JC,Tomkinson AE,Osley MA

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    journal_title:Nucleic acids research

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    authors: Morvan F,Rayner B,Imbach JL,Chang DK,Lown JW

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  • Comparison of approaches for rational siRNA design leading to a new efficient and transparent method.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Matveeva O,Nechipurenko Y,Rossi L,Moore B,Saetrom P,Ogurtsov AY,Atkins JF,Shabalina SA

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    journal_title:Nucleic acids research

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    更新日期:2007-07-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Santagati F,Botta E,Stefanini M,Pedrini AM

    更新日期:2001-04-01 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kasai H,Shindo-Okada N,Noguchi S,Nishimura S

    更新日期:1979-09-11 00:00:00

  • Characterization of a human cDNA encoding a widely expressed and highly conserved cysteine-rich protein with an unusual zinc-finger motif.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Liebhaber SA,Emery JG,Urbanek M,Wang XK,Cooke NE

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lo Conte L,Ailey B,Hubbard TJ,Brenner SE,Murzin AG,Chothia C

    更新日期:2000-01-01 00:00:00