Abstract：
:Human 2'-5' oligoadenylate synthetase-1 (OAS1) is central in innate immune system detection of cytoplasmic double-stranded RNA (dsRNA) and promotion of host antiviral responses. However, the molecular signatures that promote OAS1 activation are currently poorly defined. We show that the 3'-end polyuridine sequence of viral and cellular RNA polymerase III non-coding transcripts is critical for their optimal activation of OAS1. Potentiation of OAS1 activity was also observed with a model dsRNA duplex containing an OAS1 activation consensus sequence. We determined that the effect is attributable to a single appended 3'-end residue, is dependent upon its single-stranded nature with strong preference for pyrimidine residues and is mediated by a highly conserved OAS1 residue adjacent to the dsRNA binding surface. These findings represent discovery of a novel signature for OAS1 activation, the 3'-single-stranded pyrimidine (3'-ssPy) motif, with potential functional implications for OAS1 activity in its antiviral and other anti-proliferative roles.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Vachon VK,Calderon BM,Conn GLdoi
10.1093/nar/gku1289subject
Has Abstractpub_date
2015-01-01 00:00:00pages
544-52issue
1eissn
0305-1048issn
1362-4962pii
gku1289journal_volume
43pub_type
杂志文章abstract：:The Mouse Genome Database (MGD, http://www.informatics.jax.org/), integrates genetic, genomic and phenotypic information about the laboratory mouse, a primary animal model for studying human biology and disease. Information in MGD is obtained from diverse sources, including the scientific literature and external datab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn886
更新日期：2009-01-01 00:00:00
abstract：:ArrayExpress is a public repository for microarray data that supports the MIAME (Minimum Information About a Microarray Experiment) requirements and stores well-annotated raw and normalized data. As of November 2004, ArrayExpress contains data from approximately 12,000 hybridizations covering 35 species. Data can be s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki056
更新日期：2005-01-01 00:00:00
abstract：:Two new crescent-shaped unsymmetrical cyanine dyes have been synthesised and their interactions with DNA have been investigated by different spectroscopic methods. These dyes are analogues to a minor groove binding unsymmetrical cyanine dye, BEBO, recently reported by us. In this dye, the structure of the known interc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg821
更新日期：2003-11-01 00:00:00
abstract：:DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore dev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.11.1959
更新日期：1994-06-11 00:00:00
abstract：:In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.8.999
更新日期：1974-08-01 00:00:00
abstract：:We have previously shown that hedamycin, a GC-rich DNA-binding antitumor agent, downregulates survivin transcription (Wu et al. (2005) Molecular mechanism of inhibition of survivin transcription by the GC-rich sequence selective DNA-binding antitumor agent, hedamycin: evidence of survivin downregulation associated wit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm149
更新日期：2007-01-01 00:00:00
abstract：:The compilation of 5S rRNA and 5S rRNA gene nucleotide sequences as of 30 September 1996, contains a total of 1661 primary structures of 5S rRNAs or their genes, which is an increase of 928 new sequence entries over the last compilation. It covers sequences from 54 archaea, 449 eubacteria, 34 plastids, nine mitochondr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.1.96
更新日期：1997-01-01 00:00:00
abstract：:We took advantage of the unusual genomic organization of the ciliate Oxytricha trifallax to screen for eukaryotic non-coding RNA (ncRNA) genes. Ciliates have two types of nuclei: a germ line micronucleus that is usually transcriptionally inactive, and a somatic macronucleus that contains a reduced, fragmented and rear...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr501
更新日期：2011-09-01 00:00:00
abstract：:Amino acid sequences of 2 giant non-structural polyproteins (F1 and F2) of infectious bronchitis virus (IBV), a member of Coronaviridae, were compared, by computer-assisted methods, to sequences of a number of other positive strand RNA viral and cellular proteins. By this approach, juxtaposed putative RNA-dependent RN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.12.4847
更新日期：1989-06-26 00:00:00
abstract：:It has been proposed that DNA multiply damaged sites (MDS), where more than one moiety in a local region ( approximately 1 helical turn, 10 bp) of the DNA is damaged, are lesions of enhanced biological significance. However, other than indirect measures, there are few analytical techniques that allow direct detection ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.20.e101
更新日期：2001-10-15 00:00:00
abstract：:Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv311
更新日期：2015-05-19 00:00:00
abstract：:Nematode.net (http://nematode.net) is a publicly available resource dedicated to the study of parasitic nematodes. In 2000, the Genome Center at Washington University (GC) joined a consortium including the Nematode Genomics group in Edinburgh, and the Pathogen Sequencing Unit of the Sanger Institute to generate expres...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn744
更新日期：2009-01-01 00:00:00
abstract：:The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx688
更新日期：2017-10-13 00:00:00
abstract：:We recently identified a region of preferential replication initiation, oriGNAI3, near the 3' end of the Chinese hamster GNAI3 gene. oriGNAI3 is co-amplified in mutants selected for AMPD2 amplification, a process generating chromosomal rearrangements. In this report we have taken advantage of cell lines with truncated...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.7.1600
更新日期：1999-04-01 00:00:00
abstract：:Previous studies have shown that the yeast Candida albicans encodes a unique seryl-tRNA(CAG) that should decode the leucine codon CUG as serine. However, in vitro translation of several different CUG-containing mRNAs in the presence of this unusual seryl-tRNA(CAG) result in an apparent increase in the molecular weight...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.9.1481
更新日期：1995-05-11 00:00:00
abstract：:Kaposi's sarcoma-associated herpesvirus encodes four viral homologues to cellular interferon regulatory factors (IRFs), where the most studied is vIRF-1. Even though vIRF-1 shows sequence homology to the N-terminal DNA-binding domain (DBD) of human IRFs, a specific role for this domain in vIRF-1's function has remaine...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt082
更新日期：2013-04-01 00:00:00
abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) offers a rich platform for data sharing, publishing and archiving and a globally comprehensive data set for onward use by the scientific community. With a broad scope spanning raw sequencing reads, genome assemblies and functional annotation, the resource...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1106
更新日期：2017-01-04 00:00:00
abstract：:Human immunodeficiency virus-1 (HIV-1) leader RNA, which contains double-stranded regions due to inverted repeats, was shown to activate the dsRNA-dependent enzymes associated with the interferon system. HIV-1 leader RNA produced in vitro using SP6 RNA polymerase was characterized using probes for antisense and sense-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.3.969
更新日期：1989-02-11 00:00:00
abstract：:We have investigated the distribution of mitochondrial DNA polymorphisms in a rare maternally transmitted genetic trait that causes hypersensitivity to aminoglycoside antibiotics, in the hope that a characterization of its molecular basis might provide a molecular and cellular understanding of aminoglycoside-induced d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.18.4174
更新日期：1993-09-11 00:00:00
abstract：:RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions (UTRs). Yet, the underlying mechanisms and coding requirements for efficient co-transcriptional folding remain unclear. Traditional a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1036
更新日期：2007-01-01 00:00:00
abstract：:Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.14.5657
更新日期：1987-07-24 00:00:00
abstract：:The role of the mRNA-binding protein human antigen R (HuR) in stabilization and translation of AU-rich elements (ARE) containing mRNAs is well established. However, the trafficking of HuR and bound mRNA cargo, which comprises a fundamental requirement for the aforementioned HuR functions is only poorly understood. By ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt663
更新日期：2013-10-01 00:00:00
abstract：:Microsatellites are widely used as genetic markers because they are co-dominant, multiallelic, easily scored and highly polymorphic. A major drawback of microsatellite markers is the time and cost required to characterise them. We have developed a novel technique to reduce this cost by producing a microsatellite-rich ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.21.4369
更新日期：1996-11-01 00:00:00
abstract：:The RSC complex of Saccharomyces cerevisiae is closely related to the SWI/SNF complex. Both complexes are involved in remodeling chromatin structure and they share conserved components. The RSC proteins Sth1, Rsc8/Swh3, Sfh1 and Rsc6 are homologs of the SWI/SNF proteins Swi2/Snf2, Swi3, Snf5 and Swp73 respectively. To...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.16.3739
更新日期：1998-08-15 00:00:00
abstract：:Nuclear extracts prepared from growth hormone-secreting (GC) and prolactin-secreting (235-1) rat anterior pituitary cell lines were compared for their ability to bind to the DNA sequences conferring tissue-specificity to the expression of the rat growth hormone (rGH) gene promoter. Cell-specific differences in the int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.17.5235
更新日期：1990-09-11 00:00:00
abstract：:RNA synthesis and DNA replication cease after DNA damage. We studied RNA synthesis using an in situ run-on assay and found ribosomal RNA (rRNA) synthesis was inhibited 24 h after UV light, gamma radiation or DNA cross-linking by cisplatin in human cells. Cisplatin led to accumulation of cells in S phase. Inhibition of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt502
更新日期：2013-08-01 00:00:00
abstract：:Mammalian gene promoters for transcription by RNA polymerase II are typically organized in the following order: upstream sequence motif(s)/TATA box/initiation site. Here we report studies in which the order, orientation and DNA sequences of these three elements are varied to determine how these affect polarity of tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.24.6699
更新日期：1991-12-25 00:00:00
abstract：:Increased sequencing of microbial genomes has revealed that prevailing prokaryotic species assignments can be inconsistent with whole genome information for a significant number of species. The long-standing need for a systematic and scalable species assignment technique can be met by the genome-wide Average Nucleotid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv657
更新日期：2015-08-18 00:00:00
abstract：:Post-transcriptional modifications in archaeal RNA are known to be phylogenetically distinct but relatively little is known of tRNA from the Methanococci, a lineage of methanogenic marine euryarchaea that grow over an unusually broad temperature range. Transfer RNAs from Methanococcus vannielii, Methanococcus maripalu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.22.4699
更新日期：2001-11-15 00:00:00
abstract：:Large-scale cooperative helix opening has been previously observed in A + T rich sequences contained in supercoiled DNA molecules at elevated temperatures. Since it is well known that helix melting of linear DNA is suppressed by addition of salt, we have investigated the effects of added salts on opening transitions i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.11.2042
更新日期：1994-06-11 00:00:00
