Abstract:
:Thrombocytes express Toll-like receptors (TLRs) that detect bacterial or viral pathogens to signal the release of cytokines and mediators. We examined inflammatory responses when thrombocytes were exposed to four TLR ligands. Treatment of thrombocytes with TLR ligands demonstrates differential effects on gene expression of interleukin (IL)-6. Among the TLR ligands examined, lipopolysaccharide stimulation led to the most significant up-regulation of the IL-6 gene and a significant amount of active IL-6 in thrombocyte culture media. Lipoteichoic acid stimulation led to only marginal up-regulation of IL-6 gene expression. Although gene expression of inducible nitric oxide synthase (iNOS) did not increase due to different ligand exposure, a low level constitutive expression of iNOS was observed in all cases. Only thrombocytes treated with polyinosinic-polycytidylic acid and thymidine homopolymer phosphorothioate oligodeoxynucleotides induced rapid, significant production of nitric oxide. We also observed that thrombocytes are able to respond faster upon TLR ligand exposure compared to MQ.NCSU macrophages.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Ferdous F,Scott Tdoi
10.1016/j.dci.2014.11.019subject
Has Abstractpub_date
2015-04-01 00:00:00pages
225-30issue
2eissn
0145-305Xissn
1879-0089pii
S0145-305X(14)00301-2journal_volume
49pub_type
杂志文章abstract::Ovotransferrin (OTF) is an acute phase protein in chickens, serum levels of which increase in inflammation and infections. To understand the significance of OTF in inflammation, we studied its in vitro effects on HD11 cells, a macrophage cell line, and heterophils isolated from blood using a panel of variables indicat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(02)00028-9
更新日期:2002-11-01 00:00:00
abstract::Scanning microdensitometric analysis of Feulgen stained thymocytes from rats aged 1 to 71 days revealed the presence of a number of waves of cell proliferation which may correlate with the development of functionally distinct T-cell subpopulations. In addition, considerable numbers of hypodiploid cells were seen. It i...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(84)90021-1
更新日期:1984-01-01 00:00:00
abstract::As physical barriers that separate teleost fish from the external environment, mucosae are also active immunological sites that protect them against exposure to microbes and stressors. In mammals, the sites where antigens are sampled from mucosal surfaces and where stimulation of naïve T and B lymphocytes occurs are k...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2011.11.009
更新日期:2011-12-01 00:00:00
abstract::Dairy cows undergo a nutrient deficit immediately postpartum when lactational demands exceed nutrient intake. This occurs concurrently to an increased challenge due to bacterial and viral infections, yet ability for pathogen clearance is reduced despite a heightened and often host-damaging inflammatory response. We hy...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.11.021
更新日期:2019-04-01 00:00:00
abstract::The snail Biomphalaria glabrata kills the blood fluke Schistosoma mansoni by a mechanism involving production of hydrogen peroxide, the enzymatic product of cytosolic Cu/Zn superoxide dismutase (SOD1). This enzyme exhibits higher activity in blood cells (hemocytes) from a predominantly resistant strain of B. glabrata ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2006.12.005
更新日期:2007-01-01 00:00:00
abstract::Beta_defensin have been solely found in vertebrates until β-defensin-like peptides were described as transcript isoforms in two species of Panulirus genus. They were considered as putative antimicrobials since their biological activity have not been demonstrated. Here we purified and characterized a defensin-like pept...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2016.09.002
更新日期:2017-02-01 00:00:00
abstract::In a previous report we described the existence of two structural and antigenic variants of light chains in serum trout Igs, defined by mAbs 2H9 and 2A1. In this report, we analyse the cell distribution, the "in vitro" kinetics of production, and the ontogenic appearance of these two variants. Both variants were alrea...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(94)00058-n
更新日期:1995-01-01 00:00:00
abstract::The IMD pathway signaling plays a pivotal role in the Drosophila defense against bacteria. During the last two decades, significant progress has been made in identifying the components and deciphering the molecular mechanisms underlying this pathway, including the means of bacterial sensing and signal transduction. Wh...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2013.05.014
更新日期:2014-01-01 00:00:00
abstract::Oysters, the common name for a number of different bivalve molluscs, are the worldwide aquaculture species and also play vital roles in the function of ecosystem. As invertebrate, oysters have evolved an integrated, highly complex innate immune system to recognize and eliminate various invaders via an array of orchest...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2017.05.025
更新日期:2018-03-01 00:00:00
abstract::We investigated the effect of brown ring disease (BRD) development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio t...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2016.02.026
更新日期:2016-07-01 00:00:00
abstract::Theoretical considerations support various functions of neuroglobin (Ngb), but further studies are required for full characterization of these functions. In this study, we identified the presence of a single Ngb gene, BjNgb, in the amphioxus Branchiostoma japonicum. BjNgb was expressed in various tissues including the...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.08.004
更新日期:2017-12-01 00:00:00
abstract::The ontogeny of lymphocyte surface markers was studied in thymus, retropharyngeal lymph nodes, spleen, bone marrow, and liver of 48 bovine foetuses, 80-280 days of gestation. The markers examined were the spontaneous (E) rosette against sheep erythrocytes (SRBCs) in foetal calf serum (E/FCS), in medium supplemented wi...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(83)90018-6
更新日期:1983-04-01 00:00:00
abstract::Lectin is a protein with multiple functions. In this study, the full-length cDNA of the Agrocybe aegerita lectin (AAL) gene was cloned, recombinant AAL (AAL-His) was expressed, and the activities of AAL-His were analyzed. Northern blot analysis showed that the major AAL transcript is approximately 900 bp. Sequence ana...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.07.020
更新日期:2019-01-01 00:00:00
abstract::We previously showed that a fish interferon (IFN) regulatory factor 9 (IRF9) homologue, crucian carp Carassius auratus IRF9, displays constitutively nuclear localization and involvement in fish IFN-dependent JAK-STAT signaling; however, little is known about the expression regulation of fish IRF9. Here, we characteriz...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2013.07.017
更新日期:2013-12-01 00:00:00
abstract::In vertebrates, a total of eleven interferon (IFN) regulatory factors (IRFs), IRF1 to IRF11 are reported, with the conserved presence of IRF1 to IRF9 in all classes of vertebrates. However, IRF10 has been reported only in fish and birds, and IRF11 seems to be a fish specific IRF member. In this study, IRF11 in mandari...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103846
更新日期:2021-01-01 00:00:00
abstract::Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291bp encoding a ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2013.10.006
更新日期:2014-03-01 00:00:00
abstract::Dipteran insects, like mosquitoes, possess more than two prophenoloxidase (PPO) genes, but it is unclear whether their gene products differ in biochemical properties and physiological functions. Here, we used three Drosophila melanogaster PPOs as models to study their properties through expression in S2 cells. Our dat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2011.12.001
更新日期:2012-03-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are considered to play an important role as host-defense molecules in both vertebrates and invertebrates. This work was undertaken to characterize AMPs from hemocyte extracts of the small spider crab, Hyas araneus. A novel proline-arginine-rich AMP of 37 amino acids was isolated and chara...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2007.06.002
更新日期:2008-01-01 00:00:00
abstract::Developing viral vaccines through the ultraviolet (UV) inactivation of virus is promising technique since it is straightforward and economically affordable, while the resulting viruses are capable of eliciting an adequate antiviral immune response. Nodavirus (NNV) is a devastating virus that mainly affects European se...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.05.013
更新日期:2018-09-01 00:00:00
abstract::Up to now for Swine Workshop Cluster 2 (SWC2) the orthologous human CD molecule was unknown. By use of the SWC2-specific mAb b30c7 and a retroviral cDNA expression library derived from stimulated porcine peripheral blood mononuclear cells we could identify SWC2 as porcine CD27. Phenotypic analyses of lymphocytes isola...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.06.011
更新日期:2012-10-01 00:00:00
abstract::Thioredoxin-like protein 5 (Trxlp-5) is a thioredoxin isoform associated with cellular redox homeostasis through the activity of thiol-disulfide reductase. In our study, Trxlp-5 was identified and characterized in Pinctada fucata martensii. The expression of PmTrxlp-5 was detected in response to polyinosinic: polycyti...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103635
更新日期:2020-05-01 00:00:00
abstract::Thioredoxins (TRXs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. In this study, a thioredoxin gene was cloned from orange-spotted grouper, Epinephelus coioides (designated as Ec-TRX). The full-length cDNA of Ec-TRX was comprised of 767bp with a 327bp ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.04.011
更新日期:2012-09-01 00:00:00
abstract::TLR7 subfamily members are important pattern recognition receptors participating in the recognition of pathogen-associated molecular patterns. In this study, we successfully identified 3 members of TLR7 subfamily from the spiny eel Mastacembelus armatus (MaTLR7, MaTLR8 and MaTLR9). The amino acid sequence identities o...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2019.02.002
更新日期:2019-06-01 00:00:00
abstract::Toll-like receptors (TLRs) have been identified as pivotal sensors recognizing microbial pattern molecules in vertebrates. Whole genome analysis of the teleost Takifugu rubripes supports the existence of a fundamental family of TLR genes in fish. However, the role of the innate immune system in the context of raising ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.03.004
更新日期:2010-08-01 00:00:00
abstract::CD3 is an essential component of the TCR-CD3 complex which plays a key role in adaptive immunity. Non-mammalian CD3 complex consists of CD3γ/δ, CD3ε and CD3ζ subunits. In this study, homologues of CD3γ/δ and CD3ε (termed CiCD3γ/δ and CiCD3ε) have been identified in grass carp (Ctenopharyngodon idella). Like their coun...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103791
更新日期:2021-01-01 00:00:00
abstract::C-type lectins play important roles in innate immunity of invertebrates. In the present study, we report a novel C-type lectin, named FcLec4, from the Chinese white shrimp Fenneropenaeus chinensis. FcLec4 contains a single carbohydrate recognition domain (CRD) with a putative signal peptide. Phylogenetic analysis indi...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2009.05.004
更新日期:2009-09-01 00:00:00
abstract::In the present study, we developed an O-antigen-deficient, live, attenuated Salmonella Typhimurium (ST) strain (JOL2377) and assessed its safety, macrophage toxicity, invasion into lymphoid tissues, immunogenicity, and protection against Salmonella infection in chickens. The JOL2377 induced significantly lower cytotox...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103745
更新日期:2020-10-01 00:00:00
abstract::A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the co...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(98)00053-6
更新日期:1999-03-01 00:00:00
abstract::Immunoglobulin heavy chain (IgH) variable (V) region cDNAs from the Atlantic salmon, Salmo salar L., have been isolated and analysed with respect to diversity and transcription of the two parallel IgH isoloci in this species. A total of nine V(H) families were defined according to the 80% identity criterion, of which ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(01)00008-8
更新日期:2001-06-01 00:00:00
abstract::Cathelicidins were initially characterized as a family of antimicrobial peptides. Now it is clear that they fulfill several immune functions in addition to their antimicrobial activity. In the current work, three cDNA sequences encoding pheasant cathelicidins were cloned from a constructed bone marrow cDNA library of ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.10.004
更新日期:2011-03-01 00:00:00