Abstract：
:As the first crucial barrier in the midgut of insects, the peritrophic membrane (PM) plays an important role in preventing external invasion. PM proteins, as the major components of the PM, determine the structure and function of this membrane. A new PM protein, named LstiCBP, from the PM of Loxostege sticticalis larvae was identified using cDNA library screening. The full cDNA of LstiCBP is 2606 bp in length and contains a 2403 bp ORF that encodes an 808-amino acid preprotein with a 15-amino acid as signal peptide. The deduced protein sequence of the cDNA contains 8 cysteine-rich chitin-binding domains (CBDs). Recombinant LstiCBP was successfully expressed in BL21 cells using recombinant plasmid DNA and showed high chitin-binding activity. LstiCBP expression was detected in the midgut at both the transcriptional and translational levels; however, the biochemical and physiological functions of LstiCBP in L. sticticalis require further investigation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Yin J,Yang S,Li K,Guo W,Cao Ydoi
10.3390/ijms151019147subject
Has Abstractpub_date
2014-10-22 00:00:00pages
19147-61issue
10issn
1422-0067pii
ijms151019147journal_volume
15pub_type
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更新日期：2020-09-08 00:00:00
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