Abstract：
:The astA gene encoding an alternative sulfate transporter was originally cloned from the genome of the Japanese Aspergillus nidulans isolate as a suppressor of sulfate permease-deficient strains. Expression of the astA gene is under the control of the sulfur metabolite repression system. The encoded protein transports sulfate across the cell membrane. In this study we show that AstA, having orthologs in numerous pathogenic or endophytic fungi, has a second function and, depending on growth conditions, can be translocated into mitochondria. This effect is especially pronounced when an astA-overexpressing strain grows on solid medium at 37 °C. AstA is also recruited to the mitochondria in the presence of mitochondria-affecting compounds such as menadione or antimycin A, which are also detrimental to the growth of the astA-overexpressing strain. Disruption of the Hsp70-Porin1 mitochondrial import system either by methylene blue, an Hsp70 inhibitor, or by deletion of the porin1-encoding gene abolishes AstA translocation into the mitochondria. Furthermore, we observed altered ATP levels and sulfite oxidase activity in the astA-overexpressing strain in a manner dependent on sulfur sources. The presented data indicate that AstA is also involved in the mitochondrial sulfur metabolism in some fungi, and thereby indirectly manages redox potential and energy state.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Piłsyk S,Mieczkowski A,Golan MP,Wawrzyniak A,Kruszewska JSdoi
10.3390/ijms21207727subject
Has Abstractpub_date
2020-10-19 00:00:00issue
20issn
1422-0067pii
ijms21207727journal_volume
21pub_type
杂志文章abstract：:Aurora kinases have emerged as attractive targets for the design of anticancer drugs. 3D-QSAR (comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA)) and Surflex-docking studies were performed on a series of pyrrole-indoline-2-ones as Aurora A inhibitors. The CoMFA...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-07-11 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-08-15 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-09-22 00:00:00
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更新日期：2019-07-23 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-04-08 00:00:00
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更新日期：2019-12-07 00:00:00
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更新日期：2019-11-22 00:00:00
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更新日期：2020-07-10 00:00:00
