Abstract：
:Colon cancer is an aggressive tumor form with a poor prognosis. This study reports a comparative proteomic analysis performed by using two-dimensional differential in-gel electrophoresis (2D-DIGE) between 26 pooled colon cancer surgical tissues and adjacent non-tumoral tissues, to identify potential target proteins correlated with carcinogenesis. The DAVID functional classification tool revealed that most of the differentially regulated proteins, acting both intracellularly and extracellularly, concur across multiple cancer steps. The identified protein classes include proteins involved in cell proliferation, apoptosis, metabolic pathways, oxidative stress, cell motility, Ras signal transduction, and cytoskeleton. Interestingly, networks and pathways analysis showed that the identified proteins could be biologically inter-connected to the tumor-host microenvironment, including innate immune response, platelet and neutrophil degranulation, and hemostasis. Finally, transgelin (TAGL), here identified for the first time with four different protein species, collectively down-regulated in colon cancer tissues, emerged as a top-ranked biomarker for colorectal cancer (CRC). In conclusion, our findings revealed a different proteomic profiling in colon cancer tissues characterized by the deregulation of specific pathways involved in hallmarks of cancer. All of these proteins may represent promising novel colon cancer biomarkers and potential therapeutic targets, if validated in larger cohorts of patients.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Buttacavoli M,Albanese NN,Roz E,Pucci-Minafra I,Feo S,Cancemi Pdoi
10.3390/ijms21093096subject
Has Abstractpub_date
2020-04-28 00:00:00issue
9issn
1422-0067pii
ijms21093096journal_volume
21pub_type
杂志文章abstract：:Chronic kidney disease (CKD) is associated with atherogenic dyslipidemia. Our aim was firstly to investigate patterns of fatty acids (FA) composition through various stages of CKD, and secondly, to evaluate the effect of CKD-specific FA disturbances on the expression of genes related to lipid metabolism at a cellular ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102470
更新日期：2019-05-18 00:00:00
abstract：:Combretastatin-4 (CA-4) as a tubulin polymerization inhibitor draws extensive attentions. However, due to its weak stability of cis-olefin and poor metabolic stability, structure modifications on cis-configuration are being performed. In this work, we constructed a series of novel CA-4 analogues with linkers on olefin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051817
更新日期：2020-03-06 00:00:00
abstract：:Patients suffering from liver cirrhosis are often complicated with the formation of portosystemic collateral vessels, which is associated with the progression of a splanchnic hyperdynamic circulatory state. Alleviating pathological angiogenesis has thus been proposed to be a feasible treatment strategy. Indole-3-carbi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174161
更新日期：2019-08-26 00:00:00
abstract：:Kentucky bluegrass (KB, Poa pratensis) is one of the most widely used cool-season turfgrass species, but it is sensitive to drought stress. Molecular studies in KB are hindered by its large and complex genome structure. In this study, a comparative transcriptomic study was conducted between a short and long period of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061289
更新日期：2019-03-14 00:00:00
abstract：:Recently, we reported an inhibitory effect of guanine substitutions on the conformational switch from antiparallel to parallel quadruplexes (G4) induced by dehydrating agents. As a possible cause, we proposed a difference in the sensitivity of parallel and antiparallel quadruplexes to the guanine substitutions in the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176123
更新日期：2020-08-25 00:00:00
abstract：:It is widely accepted that chronic inflammation initiates and promotes carcinogenesis and tumor progression in various cell types. However, this paradigm has not been comprehensively investigated in melanoma. To investigate the effects of chronic inflammation on the progression of melanoma, we established a murine inf...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010169
更新日期：2019-01-04 00:00:00
abstract：:Experiments were carried out to investigate the effects of various factors on the activity and conformation of recombinant leucine aminopeptidase of Bacillus kaustophilus CCRC 11223 (BkLAP) and potential utilization of BkLAP in the hydrolysis of anchovy protein. Optimal temperature and pH of BkLAP were 70 °C and 8.0 i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12117609
更新日期：2011-01-01 00:00:00
abstract：:Protein hydroxylation is one type of post-translational modifications (PTMs) playing critical roles in human diseases. It is known that protein sequence contains many uncharacterized residues of proline and lysine. The question that needs to be answered is: which residue can be hydroxylated, and which one cannot. The ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092817
更新日期：2018-09-18 00:00:00
abstract：:The relationship between molecularly imprinted polymer (MIP) morphology and template-rebinding over a series of warfarin-imprinted methacrylic acid co(ethylene dimethacrylate) polymers has been explored. Detailed investigations of the nature of template recognition revealed that an optimal template binding was obtain...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14011207
更新日期：2013-01-09 00:00:00
abstract：:The onset and selection of resistant clones during cancer treatment with chemotherapy or targeted therapy is a major issue in the clinical management of metastatic colorectal cancer patients. It is possible that a more personalized treatment selection, using reliable response-to-therapy predictive biomarkers, could le...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18071547
更新日期：2017-07-17 00:00:00
abstract：:The incidence of inflammatory bowel disease (IBD) has increased considerably over the past few decades. In the present review, we discuss several disadvantages existing in the treatment of IBD and current understandings of the structures, sources, and natures of various kinds of non-starch polysaccharides (NSPs). Avai...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18071372
更新日期：2017-06-27 00:00:00
abstract：:Ovarian cancer is one of the commonest gynecologic malignancies, which has a poor prognosis for patients at the advanced stage. Isoliquiritigenin (ISL), an active flavonoid component of the licorice plant, previously demonstrated antioxidant, anti-inflammatory, and tumor suppressive effects. In this study, we investig...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102025
更新日期：2017-09-21 00:00:00
abstract：:As an important target for the development of novel antibiotics, UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) has been widely studied. Pyridone methylsulfone hydroxamate (PMH) compounds can effectively inhibit the catalytic activity of LpxC. In this work, the three-dimensional quantitative str...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18050761
更新日期：2017-05-06 00:00:00
abstract：:T cell engineering with antigen-specific T cell receptors (TCRs) has allowed the generation of increasingly specific, reliable, and versatile T cell products with near-physiological features. However, a broad applicability of TCR-based therapies in cancer is still limited by the restricted number of TCRs, often also o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218324
更新日期：2020-11-06 00:00:00
abstract：:Cytoplasmic aggregates and nuclear depletion of the ubiquitous RNA-binding protein TDP-43 have been described in the autoptic brain tissues of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTLD) patients and both TDP-43 loss-of-function and gain-of-function mechanisms seem to contribute to the neuro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235866
更新日期：2019-11-22 00:00:00
abstract：BACKGROUND:P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was sugg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184373
更新日期：2019-09-06 00:00:00
abstract：:A conformational analysis of nine macrocyclic thioether musks has been carried out using molecular mechanics (MMFF), density functional theory (DFT) using both B3LYP and M06 functionals, as well as Hartree-Fock and post-Hartree-Fock (MP2) ab initio methods. 6-Thia-, 10-thia- and 4-methyl-5-thia-14-tetradecananolide, 4...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10083488
更新日期：2009-08-04 00:00:00
abstract：:Diabetes is associated with a two to three-fold increase in risk of cardiovascular disease. However, intensive glucose-lowering therapy aiming at reducing HbA1c to a near-normal level failed to suppress cardiovascular events in recent randomized controlled trials. HbA1c reflects average glucose level rather than glyce...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms151018381
更新日期：2014-10-13 00:00:00
abstract：:Studies indicate the heritable nature of affective temperament, which shows personality traits predisposing to the development of mental disorders. Dopaminergic gene polymorphisms such as DRD4, COMTVal158Met, and DAT1 have been linked to affective disorders in obesity. Due to possible correlation between the aforement...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081847
更新日期：2019-04-15 00:00:00
abstract：:Ethylene is an important factor that stimulates Hevea brasiliensis to produce natural rubber. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a rate-limiting enzyme in ethylene biosynthesis. However, knowledge of the ACS gene family of H. brasiliensis is limited. In this study, nine ACS-like genes were identif...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16024136
更新日期：2015-02-16 00:00:00
abstract：:Severe hypogylcemia has been found to induce cerebral damage. While a number of illnesses can lead to hypoglycemic episodes, antidiabetic medications prescribed for glycemic control are a common cause. Considering the rising prevalence of diabetes mellitus in the population, we investigated neuroprotective strategies ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030550
更新日期：2019-01-28 00:00:00
abstract：:Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates are known to tolerate superior quinolone antimicrobials compared with other antibacterial agents. Among the clones belonging to sequence type (ST) 131 by multilocus sequence typing, the involvement of the H30-Rx subclone has been reported worldwi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205162
更新日期：2019-10-18 00:00:00
abstract：:Lysophosphatidylcholines are a group of bioactive lipids heavily investigated in the context of inflammation and atherosclerosis development. While present in plasma during physiological conditions, their concentration can drastically increase in certain inflammatory states. Lysophosphatidylcholines are widely regarde...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21124501
更新日期：2020-06-24 00:00:00
abstract：:Two-component systems (TCS) in plants have evolved into a more complicated multi-step phosphorelay (MSP) pathway, which employs histidine kinases (HKs), histidine-containing phosphotransfer proteins (HPts), and response regulators (RRs) to regulate various aspects of plant growth and development. How plants perceive t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21144898
更新日期：2020-07-11 00:00:00
abstract：:Salinity is considered as one of the most important abiotic challenges that affect crop productivity. Plant hormones, including salicylic acid (SA), are key factors in the defence signalling output triggered during plant responses against environmental stresses. We have previously reported in peach a new SA biosynthet...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113519
更新日期：2018-11-08 00:00:00
abstract：:Pulses are the main source of protein and minerals in the vegetarian diet. These are primarily cultivated on marginal lands with few inputs in several resource-poor countries of the world, including several in South Asia. Their cultivation in resource-scarce conditions exposes them to various abiotic and biotic stress...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20122971
更新日期：2019-06-18 00:00:00
abstract：:Metal tolerance proteins (MTPs) are plant divalent cation transporters that play important roles in plant metal tolerance and homeostasis. Poplar is an ideal candidate for the phytoremediation of heavy metals because of its numerous beneficial attributes. However, the definitive phylogeny and heavy metal transport mec...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051680
更新日期：2020-02-29 00:00:00
abstract：:The interactions between peptides and lipids are of fundamental importance in the functioning of numerous membrane-mediated cellular processes including antimicrobial peptide action, hormone-receptor interactions, drug bioavailability across the blood-brain barrier and viral fusion processes. Moreover, a major goal of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918758
更新日期：2013-09-12 00:00:00
abstract：:A series of gold(I) complexes of the general composition [Au(naza)(PPh₃)] (1-8) was prepared and thoroughly characterized (e.g., electrospray ionization (ESI) mass spectrometry and multinuclear nuclear magnetic resonance (NMR) spectroscopy). The N1-deprotonated anions of 7-azaindole or its derivatives (naza) are coord...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122084
更新日期：2016-12-11 00:00:00
abstract：:Ammonium trichloro[1,2-ethanediolato-O,O']-tellurate (AS101) is the most important synthetic Te compound from the standpoint of its biological activity. It is a potent immunomodulator with a variety of potential therapeutic applications and antitumoral action in several preclinical and clinical studies. An experimenta...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15023287
更新日期：2014-02-21 00:00:00
