Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester.

Abstract:

AIM:To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE). METHODS:Protein profiles of human colorectal cancer SW480 cells treated with or without CAPE were analysed using a two-dimensional (2D) electrophoresis gel-based proteomics approach. After electrophoresis, the gels were stained with Coomassie brilliant blue R-250. Digital images were taken with a GS-800 Calibrated Densitometer, and image analysis was performed using PDQuest 2-D Analysis software. The altered proteins following CAPE treatment were further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The identified proteins were validated by Western blot and immunofluorescence assay. RESULTS:CAPE induced human colorectal cancer cell apoptosis. Four up-regulated proteins and seven down-regulated proteins in colorectal cancer cells treated with CAPE were found. The identified down-regulated proteins in CAPE-treated colorectal cancer cells were Triosephosphate Isomerase (Tim), Proteasome subunit alpha 4 (PSMA4) protein, Guanine nucleotide binding protein beta, Phosphoserine aminotransferase 1 (PSAT1), PSMA1, Myosin XVIIIB and Tryptophanyl-tRNA synthetase. Notably, CAPE treatment led to the down-regulation of PSAT1 and PSMA1, two proteins that have been implicated in tumorigenesis. The identified up-regulated proteins were Annexin A4, glyceraldehyde-3-phosphate dehydrogenase, Glucosamine-6-phosphate deaminase 1 (GNPDA1), and Glutathione peroxidase (GPX-1). Based on high match scores and potential role in cell growth control, PSMA1, PSAT1, GNPDA1 and GPX-1 were further validated by Western blotting and immunofluorescence assay. PSMA1 and PSAT1 were down-regulated, while GNPDA1 and GPX-1 were up-regulated in CAPE-treated colorectal cancer cells. CONCLUSION:These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.

journal_name

World J Gastroenterol

authors

He YJ,Li WL,Liu BH,Dong H,Mou ZR,Wu YZ

doi

10.3748/wjg.v20.i33.11840

subject

Has Abstract

pub_date

2014-09-07 00:00:00

pages

11840-9

issue

33

eissn

1007-9327

issn

2219-2840

journal_volume

20

pub_type

杂志文章
  • Gastric malignancies in Northern Jordan with special emphasis on descriptive epidemiology.

    abstract:AIM:To study the epidemiology of gastric malignancies in Jordan as a model for Middle East countries where such data is scarce. METHODS:Pertinent epidemiological and clinicopathological data for 201 patients with gastric malignancy in north of Jordan between 1991 and 2001 were analyzed. RESULTS:Male: female ratio was...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i15.2174

    authors: Bani-Hani KE,Yaghan RJ,Heis HA,Shatnawi NJ,Matalka II,Bani-Hani AM,Gharaibeh KA

    更新日期:2004-08-01 00:00:00

  • Two novel VPS33B mutations in a patient with arthrogryposis, renal dysfunction and cholestasis syndrome in mainland China.

    abstract::Arthrogryposis, renal dysfunction and cholestasis (ARC) syndrome is a rare genetic disorder and has not been described in China. We present a female infant with neonatal intrahepatic cholestasis from a Chinese family with ARC syndrome. All 23 coding exons and flanking introns of the VPS33B gene were amplified and sequ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v20.i1.326

    authors: Li LT,Zhao J,Chen R,Wang JS

    更新日期:2014-01-07 00:00:00

  • Dihydromyricetin-mediated inhibition of the Notch1 pathway induces apoptosis in QGY7701 and HepG2 hepatoma cells.

    abstract:AIM:To investigate whether Dihydromyricetin (DHM) inhibits cell proliferation and promotes apoptosis by downregulating Notch1 expression. METHODS:The correlation between Notch1 and Hes1 (a Notch1 target molecule) expression in hepatoma samples was confirmed by qRT-PCR. In addition, MTT assays, flow cytometry and TUNEL...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v23.i34.6242

    authors: Lu CJ,He YF,Yuan WZ,Xiang LJ,Zhang J,Liang YR,Duan J,He YH,Li MY

    更新日期:2017-09-14 00:00:00

  • Clinical significance of expression of apoptotic signal proteins in gastric carcinoma tissue.

    abstract:AIM:To evaluate the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFkappaB in gastric carcinoma tissues and their clinical significance. METHODS:Western blot immune trace method was adopted to detect the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFkappaB in 55 tissue s...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i25.3846

    authors: Zhao XH,Gu SZ,Tian HG,Quan P,Pan BR

    更新日期:2005-07-07 00:00:00

  • Translational pancreatic cancer research: A comparative study on patient-derived xenograft models.

    abstract:AIM:To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice. METHODS:This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v24.i7.794

    authors: Rubio-Manzanares Dorado M,Marín Gómez LM,Aparicio Sánchez D,Pereira Arenas S,Praena-Fernández JM,Borrero Martín JJ,Farfán López F,Gómez Bravo MÁ,Muntané Relat J,Padillo Ruiz J

    更新日期:2018-02-21 00:00:00

  • Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.

    abstract:AIM:To observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs. METHODS:Wistar rat endotoxemia model was established first by injection of a dose of LPS (5mg/kg, Esche...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v8.i3.551

    authors: Gong JP,Dai LL,Liu CA,Wu CX,Shi YJ,Li SW,Li XH

    更新日期:2002-06-01 00:00:00

  • Current concepts and controversies in the treatment of alcoholic hepatitis.

    abstract::The treatment of alcoholic hepatitis remains one of the most debated topics in medicine and a field of continued research. In this review, we discuss the evolution of scoring systems, including the recent development of the Glasgow alcoholic hepatitis score, role of liver biopsy and current treatment interventions. St...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v12.i43.6909

    authors: Rongey C,Kaplowitz N

    更新日期:2006-11-21 00:00:00

  • Transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma.

    abstract:AIM:To explore the transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma to understand mechanisms of the signaling pathway at so gene level. METHODS:Total RNA was isolated from human colorectal carcinoma cell line LoVo treated with HGF/SF (80 ng/L) for 48 h. Fluorescent probe...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v9.i8.1734

    authors: Li XN,Ding YQ,Liu GB

    更新日期:2003-08-01 00:00:00

  • Requirements for transfusion and postoperative outcomes in orthotopic liver transplantation: a meta-analysis on aprotinin.

    abstract:AIM:To study the effect of aprotinin used in orthotopic liver transplantation (OLT) on the intraoperative requirement for blood products and on the incidence of laparotomy for bleeding, thrombotic events and mortality. METHODS:A systematic review of the literature in the electronic database Medline and the Clinic Tria...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,meta分析,评审

    doi:10.3748/wjg.14.1425

    authors: Liu CM,Chen J,Wang XH

    更新日期:2008-03-07 00:00:00

  • Loss of interstitial cells of Cajal network in severe idiopathic gastroparesis.

    abstract:AIM:To report a case of severe idiopathic gastroparesis in complete absence of Kit-positive gastric interstitial cells of Cajal (ICC). METHODS:Gastric tissue from a patient with severe idiopathic gastroparesis unresponsive to medical treatment and requiring surgery was analyzed by conventional histology and immunohist...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v12.i38.6172

    authors: Battaglia E,Bassotti G,Bellone G,Dughera L,Serra AM,Chiusa L,Repici A,Mioli P,Emanuelli G

    更新日期:2006-10-14 00:00:00

  • Stronger inhibition of gastric acid secretion by lafutidine, a novel H2 receptor antagonist, than by the proton pump inhibitor lansoprazole.

    abstract:AIM:To compare the antisecretory activity and plasma drug concentrations of a single oral dose of 10 mg lafutidine, a novel H2 receptor antagonist, with those of the proton pump inhibitor lansoprazole (LPZ) 30 mg. METHODS:Ten volunteers without H pylori infection participated in this crossover study comparing lafutidi...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,随机对照试验

    doi:10.3748/wjg.14.2406

    authors: Yamagishi H,Koike T,Ohara S,Horii T,Kikuchi R,Kobayashi S,Abe Y,Iijima K,Imatani A,Suzuki K,Hishinuma T,Goto J,Shimosegawa T

    更新日期:2008-04-21 00:00:00

  • Basaloid squamous carcinoma of esophagus:a clinicopathological, immunohistochemical and electron microscopic study of sixteen cases.

    abstract::AIM:To further clarify the clinicopathological, immunohistochemical and electron microscopic features, and prognostic aspect of basaloid squamous carcinoma (BSC), a rare esophageal carcinoma.METHODS:We reviewed 763 documented cases of esophageal malignancies from year (1977-1996) from our hospital, and discovered 16 (...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v4.i5.397

    authors: Zhang XH,Sun GQ,Zhou XJ,Guo HF,Zhang TH

    更新日期:1998-10-01 00:00:00

  • Evaluation of therapeutic effectiveness of (131)I-antiEGFR-BSA-PCL in a mouse model of colorectal cancer.

    abstract:AIM:To investigate the biological effects of internal irradiation, and the therapeutic effectiveness was assessed of (131)I-labeled anti-epidermal growth factor receptor (EGFR) liposomes, derived from cetuximab, when used as a tumor-targeting carrier in a colorectal cancer mouse model. METHODS:We described the liposom...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i14.3758

    authors: Li W,Ji YH,Li CX,Liu ZY,Li N,Fang L,Chang J,Tan J

    更新日期:2016-04-14 00:00:00

  • Distribution and effects of polymorphic RANTES gene alleles in HIV/HCV coinfection -- a prospective cross-sectional study.

    abstract:AIM:Chemokines and their receptors are crucial for immune responses in HCV and HIV infection. RANTES gene polymorphisms lead to altered gene expression and influence the natural course of HIV infection. Therefore, these mutations may also affect the course of HIV/HCV coinfection. METHODS:We determined allele frequenci...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v11.i48.7631

    authors: Ahlenstiel G,Iwan A,Nattermann J,Bueren K,Rockstroh JK,Brackmann HH,Kupfer B,Landt O,Peled A,Sauerbruch T,Spengler U,Woitas RP

    更新日期:2005-12-28 00:00:00

  • Relationship between RGS5 expression and differentiation and angiogenesis of gastric carcinoma.

    abstract:AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD). METHODS:Expression of RGS5 and CD34 were examined in 76 cases of gastric carcinoma, including 22 cases with lymph node metastasis and 54 cases without l...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v16.i44.5642

    authors: Wang JH,Huang WS,Hu CR,Guan XX,Zhou HB,Chen LB

    更新日期:2010-11-28 00:00:00

  • Use of chorioamniotic membrane instead of bogota bag in open abdomen: how i do it?

    abstract::It is one of the most important problems for general surgeons to decide which operation should be undertaken on patients with intra-abdominal infection, especially those with concomitant abdominal hypertension. Recently, closure techniques using prosthetic meshes in order to retain abdominal tension and to control sep...

    journal_title:World journal of gastroenterology

    pub_type: 信件

    doi:10.3748/wjg.14.815

    authors: Tekin S,Tekin A,Kucukkartallar T,Cakir M,Kartal A

    更新日期:2008-02-07 00:00:00

  • Hematologic manifestations of Helicobacter pylori infection.

    abstract::Helicobacter pylori (H. pylori) is the most common infection in humans, with a marked disparity between developed and developing countries. Although H. pylori infections are asymptomatic in most infected individuals, they are intimately related to malignant gastric conditions such as gastric cancer and gastric mucosa-...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i36.12818

    authors: Campuzano-Maya G

    更新日期:2014-09-28 00:00:00

  • Human endostatin gene transfer, either naked or with liposome, has the same inhibitory effect on growth of mouse liver tumor cells in vivo.

    abstract:AIM:To explore a safe and efficient strategy of tumor therapy using anti-angiogenetic agents. METHODS:Endostatin gene with a signal sequence of human IgGgamma chain was amplified by PCR and cloned into pVAX1 plasmid which was the only vector authorized by FDA in clinical trial to construct a recombinant plasmid named ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v10.i19.2874

    authors: Ma CH,Zhang Y,Wang XY,Gao LF,Liu H,Guo C,Liu SX,Cao YL,Zhang LN,Sun WS

    更新日期:2004-10-01 00:00:00

  • Antifibrotic effects of green tea on in vitro and in vivo models of liver fibrosis.

    abstract:AIM:To examine the protective effect of green tea extract (GT) on hepatic fibrosis in vitro and in vivo in dimethylnitrosamine (DMN)-induced rats. METHODS:HSC-T6, a rat hepatic stellate cell line, was used as an in vitro assay system. Cell proliferation, collagen content, and type 1 collagen expression were examined i...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.15.5200

    authors: Kim HK,Yang TH,Cho HY

    更新日期:2009-11-07 00:00:00

  • Probiotic monotherapy and Helicobacter pylori eradication: A systematic review with pooled-data analysis.

    abstract:AIM:To define probiotic monotherapy effect on Helicobacter pylori (H. pylori) status by performing a systematic review. METHODS:Methods of analysis and inclusion criteria were based on PRISMA recommendations. Relevant publications were identified by searching PubMed, MEDLINE, Science Direct, and EMBASE. The end-point ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,meta分析,评审

    doi:10.3748/wjg.v24.i1.139

    authors: Losurdo G,Cubisino R,Barone M,Principi M,Leandro G,Ierardi E,Di Leo A

    更新日期:2018-01-07 00:00:00

  • Current management of hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and leading cause of death among patients with cirrhosis. Treatment guidelines are based according to the Barcelona Clinic Liver Cancer staging system. The choice among therapeutic options that include liver resection, liver transplantation, loco...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i30.10223

    authors: Tabrizian P,Roayaie S,Schwartz ME

    更新日期:2014-08-14 00:00:00

  • Hemorrhoidectomy - making sense of the surgical options.

    abstract::While debate continues as to which is the best surgical method for the treatment of hemorrhoids, none of the currently available surgical methods approach the ideal surgical option, which is one that is effective while being safe and painless. In reality, the less painful the procedure, the more likely it is to be ass...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i45.16976

    authors: Yeo D,Tan KY

    更新日期:2014-12-07 00:00:00

  • Noninvasive assessment of liver fibrosis in patients with chronic hepatitis B.

    abstract::Infection with hepatitis B virus is an important health problem worldwide: it affects more than 350 million people and is a leading cause of liver-related morbidity, accounting for 1 million deaths annually. Hepatic fibrosis is a consequence of the accumulation of extracellular matrix components in the liver. An accur...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i34.12031

    authors: Enomoto M,Morikawa H,Tamori A,Kawada N

    更新日期:2014-09-14 00:00:00

  • Expression of the c-erbB-2 proto-oncogene product in gastric carcinoma and precancerous lesions.

    abstract:AIM:To study the relationship between the expression of the c-erbB-2 proto-oncogene product with gastric mucosal carcinogenesis and the behavior of gastric carcinoma. METHODS:Specimens from nine normal gastric mucosa, 23 gastric mucosal dysplasia (10 slight, six moderate, seven severe), 18 early gastric carcinoma, and...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v3.i2.122

    authors: Mi JQ,Yang SQ,Shen MC

    更新日期:1997-06-15 00:00:00

  • Guillain-Barré syndrome following hepatitis E.

    abstract::Guillain-Barré syndrome (GBS) is often triggered by a preceding bacterial or viral infection. Occasionally, it has been observed in association with acute hepatitis A, B and C, and three cases have been previously described in India in which GBS was associated with acute hepatitis E. A molecular mimicry mechanism is s...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.15.1645

    authors: Loly JP,Rikir E,Seivert M,Legros E,Defrance P,Belaiche J,Moonen G,Delwaide J

    更新日期:2009-04-07 00:00:00

  • Characterisation of colonic dysplasia-like epithelial atypia in murine colitis.

    abstract:AIM:To determine if exacerbation of pre-existing chronic colitis in Winnie (Muc2 mutant) mice induces colonic dysplasia. METHODS:Winnie mice and C57BL6 as a genotype control, were administered 1% w/v dextran sulphate sodium (DSS) orally, followed by drinking water alone in week-long cycles for a total of three cycles....

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v22.i37.8334

    authors: Randall-Demllo S,Fernando R,Brain T,Sohal SS,Cook AL,Guven N,Kunde D,Spring K,Eri R

    更新日期:2016-10-07 00:00:00

  • Signaling pathway/molecular targets and new targeted agents under development in hepatocellular carcinoma.

    abstract::Advances in molecular cell biology over the last decade have clarified the mechanisms involved in cancer growth, invasion, and metastasis, and enabled the development of molecular-targeted agents. To date, sorafenib is the only molecular-targeted agent whose survival benefit has been demonstrated in two global phase I...

    journal_title:World journal of gastroenterology

    pub_type: 社论,评审

    doi:10.3748/wjg.v18.i42.6005

    authors: Kudo M

    更新日期:2012-11-14 00:00:00

  • Diagnosis and evaluation of gastric cancer by positron emission tomography.

    abstract::Gastric cancer is the second leading cause of cancer mortality worldwide. The diagnosis of gastric cancer has been significantly improved with the broad availability of gastrointestinal endoscopy. Effective technologies for accurate staging and quantitative evaluation are still in demand to merit reasonable treatment ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3748/wjg.v20.i16.4574

    authors: Wu CX,Zhu ZH

    更新日期:2014-04-28 00:00:00

  • Impact of B-mode-ultrasound-guided transhepatic and transperitoneal cholecystostomy tube placement on laparoscopic cholecystectomy.

    abstract:BACKGROUND:B-mode-ultrasound-guided percutaneous cholecystostomy (PC) may be performed by a transhepatic or transperitoneal approach, called percutaneous transhepatic gallbladder drainage (PHGD) and percutaneous transperitoneal gallbladder drainage (PPGD), respectively. We compared the impact of PC related to the route...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.v26.i36.5498

    authors: Liu P,Liu C,Wu YT,Zhu JY,Zhao WC,Li JB,Zhang H,Yang YX

    更新日期:2020-09-28 00:00:00

  • Increased N-terminal pro-brain natriuretic peptide level predicts atrial fibrillation after surgery for esophageal carcinoma.

    abstract:AIM:To evaluate the value of plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) level for predicting postoperative atrial fibrillation (AF) in patients undergoing surgery for esophageal carcinoma. METHODS:NT-proBNP levels were measured in 142 patients 24 h before and 1 h after surgery for esophageal carcinoma...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3748/wjg.14.2582

    authors: Hou JL,Gao K,Li M,Ma JY,Shi YK,Wang Y,Zhao YF

    更新日期:2008-04-28 00:00:00