Abstract:
:Conserved RNA secondary structures were predicted in the nucleoprotein (NP) segment of the influenza A virus genome using comparative sequence and structure analysis. A number of structural elements exhibiting nucleotide covariations were identified over the whole segment length, including protein-coding regions. Calculations of mutual information values at the paired nucleotide positions demonstrate that these structures impose considerable constraints on the virus genome evolution. Functional importance of a pseudoknot structure, predicted in the NP packaging signal region, was confirmed by plaque assays of the mutant viruses with disrupted structure and those with restored folding using compensatory substitutions. Possible functions of the conserved RNA folding patterns in the influenza A virus genome are discussed.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Gultyaev AP,Tsyganov-Bodounov A,Spronken MI,van der Kooij S,Fouchier RA,Olsthoorn RCdoi
10.4161/rna.29730subject
Has Abstractpub_date
2014-01-01 00:00:00pages
942-52issue
7eissn
1547-6286issn
1555-8584pii
29730journal_volume
11pub_type
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