Abstract:
:The p75 neurotrophin receptor, a member of the tumor necrosis factor receptor superfamily, is required as a co-receptor for the Nogo receptor (NgR) to mediate the activity of myelin-associated inhibitors such as Nogo, MAG, and OMgp. p45/NRH2/PLAIDD is a p75 homologue and contains a death domain (DD). Here we report that p45 markedly interferes with the function of p75 as a co-receptor for NgR. P45 forms heterodimers with p75 and thereby blocks RhoA activation and inhibition of neurite outgrowth induced by myelin-associated inhibitors. p45 binds p75 through both its transmembrane (TM) domain and DD. To understand the underlying mechanisms, we have determined the three-dimensional NMR solution structure of the intracellular domain of p45 and characterized its interaction with p75. We have identified the residues involved in such interaction by NMR and co-immunoprecipitation. The DD of p45 binds the DD of p75 by electrostatic interactions. In addition, previous reports suggested that Cys257 in the p75 TM domain is required for signaling. We found that the interaction of the cysteine 58 of p45 with the cysteine 257 of p75 within the TM domain is necessary for p45-p75 heterodimerization. These results suggest a mechanism involving both the TM domain and the DD of p45 to regulate p75-mediated signaling.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Vilar M,Sung TC,Chen Z,García-Carpio I,Fernandez EM,Xu J,Riek R,Lee KFdoi
10.1371/journal.pbio.1001918subject
Has Abstractpub_date
2014-08-05 00:00:00pages
e1001918issue
8eissn
1544-9173issn
1545-7885pii
PBIOLOGY-D-14-01553journal_volume
12pub_type
杂志文章相关文献
PLOS BIOLOGY文献大全abstract::The functional consequences of long-range nuclear reorganization were studied in a cell-by-cell analysis of gene expression and long-range chromosomal interactions in the Drosophila eye and eye imaginal disk. Position-effect variegation was used to stochastically perturb gene expression and probe nuclear reorganizatio...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030067
更新日期:2005-03-01 00:00:00
abstract::Protein modifications play a major role for most biological processes in living organisms. Amino-terminal acetylation of proteins is a common modification found throughout the tree of life: the N-terminus of a nascent polypeptide chain becomes co-translationally acetylated, often after the removal of the initiating me...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000236
更新日期:2009-11-01 00:00:00
abstract::During in vitro fertilisation (IVF), pharmacological activation of the murine X chromosome-encoded receptor proteins Toll-like receptor (TLR) 7 and TLR8 reportedly results in male-biased litters by selectively disrupting the motility of X-bearing sperm cells. Thus-in the context of agonist treatment during IVF-these r...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000663
更新日期:2020-03-23 00:00:00
abstract::The ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. Among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. Polyamine deficiency or excess has detr...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002454
更新日期:2016-05-04 00:00:00
abstract::Planarians display remarkable plasticity in maintenance of their germline, with the ability to develop or dismantle reproductive tissues in response to systemic and environmental cues. Here, we investigated the role of G protein-coupled receptors (GPCRs) in this dynamic germline regulation. By genome-enabled receptor ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002457
更新日期:2016-05-10 00:00:00
abstract::The most common form of human autosomal dominant hereditary spastic paraplegia (AD-HSP) is caused by mutations in the SPG4 (spastin) gene, which encodes an AAA ATPase closely related in sequence to the microtubule-severing protein Katanin. Patients with AD-HSP exhibit degeneration of the distal regions of the longest ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020429
更新日期:2004-12-01 00:00:00
abstract::In this Open Highlight, Senior Editor Lauren Richardson features exciting new Open Access research into how species evolve their characteristic traits. ...
journal_title:PLoS biology
pub_type: 评论,杂志文章
doi:10.1371/journal.pbio.3000020
更新日期:2018-10-04 00:00:00
abstract::Malaria parasite transmission requires differentiation of male and female gametocytes into gametes within a mosquito following a blood meal. A mosquito-derived molecule, xanthurenic acid (XA), can trigger gametogenesis, but the signalling events controlling this process in the human malaria parasite Plasmodium falcipa...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0060139
更新日期:2008-06-03 00:00:00
abstract::In nature, Escherichia coli are exposed to harsh and non-ideal growth environments-nutrients may be limiting, and cells are often challenged by oxidative stress. For E. coli cells confronting these realities, there appears to be a link between oxidative stress, methionine availability, and the enzyme that catalyzes th...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020336
更新日期:2004-11-01 00:00:00
abstract::Protein kinase B (PKB/Akt) belongs to the AGC superfamily of related serine/threonine protein kinases. It is a key regulator downstream of various growth factors and hormones and is involved in malignant transformation and chemo-resistance. Full-length PKB protein has not been crystallised, thus studying the molecular...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000017
更新日期:2009-01-20 00:00:00
abstract::Alpha thalassemia/mental retardation syndrome X-linked chromatin remodeler (ATRX), a DAXX (death domain-associated protein) interacting protein, is often lost in cells using the alternative lengthening of telomeres (ALT) pathway, but it is not known how ATRX loss leads to ALT. We report that ATRX deletion from mouse c...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000594
更新日期:2020-01-02 00:00:00
abstract::A vast and rich body of information has grown up as a result of the world's enthusiasm for 'omics technologies. Finding ways to describe and make available this information that maximise its usefulness has become a major effort across the 'omics world. At the heart of this effort is the Genomic Standards Consortium (G...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001088
更新日期:2011-06-01 00:00:00
abstract::Two-component signal transduction pathways comprising histidine protein kinases (HPKs) and their response regulators (RRs) are widely used to control bacterial responses to environmental challenges. Some bacteria have over 150 different two-component pathways, and the specificity of the phosphotransfer reactions withi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000306
更新日期:2010-02-09 00:00:00
abstract::Phenotypic heterogeneity can confer clonal groups of organisms with new functionality. A paradigmatic example is the bistable expression of virulence genes in Salmonella typhimurium, which leads to phenotypically virulent and phenotypically avirulent subpopulations. The two subpopulations have been shown to divide lab...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001928
更新日期:2014-08-19 00:00:00
abstract::Total internal reflection fluorescence microscopy (TIR-FM) has become a powerful tool for studying clathrin-mediated endocytosis. However, due to difficulties in tracking and quantifying their heterogeneous dynamic behavior, detailed analyses have been restricted to a limited number of selected clathrin-coated pits (C...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000057
更新日期:2009-03-17 00:00:00
abstract::During gestation, uterine smooth muscle cells transition from a state of quiescence to one of contractility, but the molecular mechanisms underlying this transition at a genomic level are not well-known. To better understand these events, we evaluated the epigenetic landscape of the mouse myometrium during the pregnan...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000710
更新日期:2020-07-15 00:00:00
abstract::The gene GAD2 encoding the glutamic acid decarboxylase enzyme (GAD65) is a positional candidate gene for obesity on Chromosome 10p11-12, a susceptibility locus for morbid obesity in four independent ethnic populations. GAD65 catalyzes the formation of gamma-aminobutyric acid (GABA), which interacts with neuropeptide Y...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0000068
更新日期:2003-12-01 00:00:00
abstract::Zika virus (ZIKV) is a health burden due to the severe neurological abnormalities that arise after congenital infection. Although multiple experimental studies have linked ZIKV with neural birth defects, the scientific community has not been able to fully explain why Congenital Zika Syndrome (CZS) was only apparent af...
journal_title:PLoS biology
pub_type: 杂志文章,评审
doi:10.1371/journal.pbio.2006592
更新日期:2018-08-24 00:00:00
abstract::Synaptic plasticity is widely believed to constitute a key mechanism for modifying functional properties of neuronal networks. This belief implicitly implies, however, that synapses, when not driven to change their characteristics by physiologically relevant stimuli, will maintain these characteristics over time. How ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000136
更新日期:2009-06-23 00:00:00
abstract::A cell is a minifactory in which structures and molecules are assembled, rearranged, disassembled, packaged, sorted, and transported. Because cellular structures and molecules are invisible to the human eye, students often have difficulty conceptualizing the dynamic nature of cells that function at multiple scales acr...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002118
更新日期:2015-04-09 00:00:00
abstract::Every instant of perception depends on a cascade of brain processes calibrated to the history of sensory and decisional events. In the present work, we show that human visual perception is constantly shaped by two contrasting forces exerted by sensory adaptation and past decisions. In a series of experiments, we used ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000144
更新日期:2019-03-05 00:00:00
abstract::Negative regulation of receptor signaling is essential for controlling cell activation and differentiation. In B-lymphocytes, the down-regulation of B-cell antigen receptor (BCR) signaling is critical for suppressing the activation of self-reactive B cells; however, the mechanism underlying the negative regulation of ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001704
更新日期:2013-11-01 00:00:00
abstract::Progress in science often begins with verbal hypotheses meant to explain why certain biological phenomena exist. An important purpose of mathematical models in evolutionary research, as in many other fields, is to act as “proof-of-concept” tests of the logic in verbal explanations, paralleling the way in which empiric...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002017
更新日期:2014-12-09 00:00:00
abstract::Chemotaxis allows bacteria to colonize their environment more efficiently and to find optimal growth conditions, and is consequently under strong evolutionary selection. Theoretical and experimental analyses of bacterial chemotaxis suggested that the pathway has been evolutionarily optimized to produce robust output u...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000171
更新日期:2009-08-01 00:00:00
abstract::The source of symmetry breaking in vertebrate oocytes is unknown. Animal-vegetal oocyte polarity is established by the Balbiani body (Bb), a conserved structure found in all animals examined that contains an aggregate of specific mRNAs, proteins, and organelles. The Bb specifies the oocyte vegetal pole, which is key t...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002335
更新日期:2016-01-07 00:00:00
abstract::Adult humans possess a sophisticated repertoire of mathematical faculties. Many of these capacities are rooted in symbolic language and are therefore unlikely to be shared with nonhuman animals. However, a subset of these skills is shared with other animals, and this set is considered a cognitive vestige of our common...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0050328
更新日期:2007-12-01 00:00:00
abstract::The signals that initiate cell invasion are not well understood, but there is increasing evidence that extracellular physical signals play an important role. Here we show that epithelial cell invasion in the intestine of zebrafish meltdown (mlt) mutants arises in response to unregulated contractile tone in the surroun...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001386
更新日期:2012-01-01 00:00:00
abstract::Metabolic reprogramming to fulfill the biosynthetic and bioenergetic demands of cancer cells has aroused great interest in recent years. However, metabolic reprogramming for cancer metastasis has not been well elucidated. Here, we screened a subpopulation of breast cancer cells with highly metastatic capacity to the l...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000872
更新日期:2020-11-13 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pbio.3000478.]. ...
journal_title:PLoS biology
pub_type: 已发布勘误
doi:10.1371/journal.pbio.3000568
更新日期:2019-11-13 00:00:00
abstract::Aminoacyl-tRNA synthetases (aaRSs) are well studied for their role in binding and charging tRNAs with cognate amino acids. Recent RNA interactome studies had suggested that these enzymes can also bind polyadenylated RNAs. Here, we explored the mRNA repertoire bound by several yeast aaRSs. RNA immunoprecipitation (RIP)...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000274
更新日期:2019-05-17 00:00:00