Abstract：
:Estetrol (E4) is a recently described natural estrogen with four hydroxyl-groups that is synthesized exclusively during pregnancy by the human fetal liver. It has important antioxidative activity. The aim of the present study was to define the importance of E4 in the attenuation of neonatal hypoxic-ischemic encephalopathy. Antioxidative effect of 650μM, 3.25mM and 6.5mM E4 on primary hippocampal cell cultures was studied before/after H202-induced oxidative stress. To examine oxidative stress and cell viability, lactate dehydrogenase activity and cell proliferation colorimetric assays were performed. To study the neuroprotective and therapeutic effects of E4 in vivo neonatal hypoxic-ischemic encephalopathy model of 7-day-old newborn rat pups was used. The neuroprotective and therapeutic effects of estetrol before/after hypoxic-ischemic insult was studied in 1mg/kg/day, 5mg/kg/day, 10mg/kg/day, 50mg/kg/day E4 pretreated/treated groups and compared with the sham and the vehicle treated groups. The body temperature of the rat pups was examined along with their body and brain weights. Brains were studied at the level of the hippocampus and cortex. Intact cell counting and expressions of microtubule-associated protein-2, doublecortin and vascular-endothelial growth factor were evaluated by histo- and immunohistochemistry. ELISAs were performed on blood samples to detect concentrations of S100B and glial fibrillary acidic protein as brain damage markers. This work reveals for the first time that E4 significantly decreases LDH activity and enhances cell proliferation in primary hippocampal neuronal cell cultures in vitro, and decreases the early gray matter loss and promotes neuro- and angiogenesis in vivo.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Tskitishvili E,Nisolle M,Munaut C,Pequeux C,Gerard C,Noel A,Foidart JMdoi
10.1016/j.expneurol.2014.07.015subject
Has Abstractpub_date
2014-11-01 00:00:00pages
298-307eissn
0014-4886issn
1090-2430pii
S0014-4886(14)00243-Xjournal_volume
261pub_type
杂志文章abstract：:An in vitro study of the nuclear magnetic resonance (NMR) response of protons in normal and pathological human skeletal muscle is presented. Muscle biopsies from 34 patients with various neuromuscular diseases and five normal subjects were studied by proton NMR techniques and the results were compared with those of li...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90159-0
更新日期：1983-07-01 00:00:00
abstract：:NMDA receptors in postmortem human spinal cord were analyzed using [3H]MK-801 ligand binding and immunoblotting with NMDA receptor subunit-specific antibodies. The average KD for [3H]MK-801 binding was 1.77 nM with a Bmax of 0.103 pmol/mg. The EC50 for stimulation of -3H-MK-801 binding with L-glutamate was 0.34 microM...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6691
更新日期：1997-12-01 00:00:00
abstract：:Diffuse axonal injury is thought to be the basis of the functional impairments stemming from mild traumatic brain injury. To examine how axons are damaged by traumatic events, such as motor vehicle accidents, falls, sports activities, or explosive blasts, we have taken advantage of the spinal cord with its extensive w...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.04.023
更新日期：2015-09-01 00:00:00
abstract：:Spinal cord injury (SCI) results in chronic pain states in which the underlying mechanism is poorly understood. To begin to explore possible mechanisms, calcitonin gene-related peptide (CGRP), a neuropeptide confined to fine primary afferent terminals in laminae I and II in the dorsal horn of the spinal cord and impli...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6608
更新日期：1997-10-01 00:00:00
abstract：:Cells dissociated from 6-day rat cerebellum were seeded on glass coverslips coated with polylysine on one half and hyaluronectin on the other. Cell attachment and neurite extension were drastically reduced on hyaluronectin-coated surfaces. ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90218-x
更新日期：1988-04-01 00:00:00
abstract：:Injury of a peripheral nerve not only leads to target denervation, but also induces massive stripping of spinal synapses on axotomized motoneurons, with disruption of spinal circuits. Even when regeneration is successful, unspecific reinnervation and the limited reconnection of the spinal circuits impair functional re...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.10.009
更新日期：2015-01-01 00:00:00
abstract：:Cordotomy-denervation interactions were studied on contractile and myofibrillar properties of slow (soleus) and fast (extensor digitorum longus) muscles of the rat. The spinal cord was transected midthoracically in neonatal (2-day-old) animals. Two months after birth, a unilateral transection of the sciatic nerve was ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90214-2
更新日期：1988-04-01 00:00:00
abstract：:Demyelinating diseases comprise a spectrum of immunopathologic syndromes in which myelin, the fatty covering of nerve cell fibers in the brain and spinal cord, is destroyed. In this study, we have shown for the first time that ocular infection of BALB/c mice with a recombinant herpes simplex virus type 1 (HSV-1) expre...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.12.004
更新日期：2005-05-01 00:00:00
abstract：:Photic entrainment of circadian rhythms in mammals is mediated through a direct retinal projection to the core region of the suprachiasmatic nucleus (SCN), the circadian clock. A proportion of this projection contains the low-affinity p75 neurotrophic receptor (p75NTR). Neonatal monosodium glutamate (MSG) treatment, w...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2002.7969
更新日期：2002-08-01 00:00:00
abstract：BACKGROUND:Cognitive impairment in humans with Alzheimer's disease (AD) and in animal models of Aβ-pathology can be ameliorated by treatments with the nuclear receptor peroxisome proliferator-activated receptor-gamma (PPARγ) agonists, such as rosiglitazone (RSG). Previously, we demonstrated that in the Tg2576 animal mo...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.05.005
更新日期：2017-09-01 00:00:00
abstract：:Penile (cavernous) nerves are readily damaged during radical prostatectomy, invariably causing impotence. Erectile function can return, however this may take months or years and capacity often remains poor. Many studies have attempted to improve penile nerve regeneration but have not explored mechanisms underlying the...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2007.05.011
更新日期：2007-09-01 00:00:00
abstract：:Second-order neurons in trigeminal subnucleus caudalis (Vc) and upper cervical spinal cord (C1) are critical for craniofacial pain processing and project rostrally to terminate in: ventral posteromedial thalamic nucleus (VPM), medial thalamic nuclei (MTN) and parabrachial nuclei (PBN). The contribution of each region ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.03.024
更新日期：2017-07-01 00:00:00
abstract：:Methylmalonic acidemia is an inherited metabolic disorder that leads to brain damage associated to the accumulation of methylmalonic acid (MMA) and impairment of energy metabolism. We demonstrate here that treatment with diazoxide, an agonist of mitochondrial ATP-sensitive K(+) channels (mitoK(ATP)), can prevent death...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2006.04.004
更新日期：2006-09-01 00:00:00
abstract：:The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF) is involved in injury-induced peripheral nerve pathology and in the generation of neuropathic pain. Here, we investigated local protein levels of the two known TNF receptors, TNF receptor 1 and 2 (TNFR1, TNFR2), on days 0, 1, 3, 7, 14, and 28 after unilat...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.11.002
更新日期：2005-03-01 00:00:00
abstract：:Using a measure of nonlinear dynamical changes we term marginal predictability, we report evidence of robust changes in this parameter on scalp EEG in a cohort of patients with medically refractory mesiobasal temporal lobe epilepsy (MBTLE). In the baseline (interictal) state there are distinct differences in this nonl...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(03)00354-6
更新日期：2003-11-01 00:00:00
abstract：:We investigated whether administration of neurotrophin-3 (NT-3) and NMDA-2D-expressing units, found previously to enhance transmission in neonatal rat spinal cord, strengthens synaptic connections in the injured neonatal cord. We employed electrophysiological methods to evaluate the strength of synaptic transmission t...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2005.10.008
更新日期：2006-02-01 00:00:00
abstract：:Thyroid hormones and spermidine, a motor neuron trophic polyamine (PA), have been shown to enhance peripheral motor nerve regeneration; however, the mechanism by which these treatment modalities exert their effect is unknown. Similarities in treatment outcome suggest that these molecules may be working via a common me...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.05.024
更新日期：2004-09-01 00:00:00
abstract：:In the cerebellum of adult-aging Ts65Dn mice, a murine model of Down syndrome, Purkinje cells undergo degeneration. Searching for the cause of Purkinje cell degeneration, we have studied the ubiquitin-proteasome system (UPS) in the cerebellum of aging Ts65Dn mice. Inhibition of UPS is sufficient to induce neuron degen...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2011.08.009
更新日期：2011-12-01 00:00:00
abstract：:Poly(ADP-ribose) polymerase (PARP) was shown to be detrimental in cerebral ischemia but the mechanisms whereby PARP is deleterious have yet to be determined. They may include a role in neutrophil infiltration known to aggravate ischemic damage. In this context, we investigated the effect of 3-aminobenzamide (3-AB), a ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/S0014-4886(03)00367-4
更新日期：2003-12-01 00:00:00
abstract：:Ocular movements of alert cats were recorded by classical electronystagmography techniques during (a) vestibular stimulation (sinusoidal rotation of the cat in complete darkness), (b) optokinetic stimulation (sinusoidal rotation of the visual surroundings around the stationary cat), (c) additive visual-vestibular stim...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90005-5
更新日期：1983-04-01 00:00:00
abstract：:Cerebral ischemia caused by loss of blood supply to the brain during cardiac arrest or stroke are major causes of death and disability. Biological sex is an important factor in predicting vulnerability of the brain to an ischemic insult, with males being at higher risk for cardio-cerebrovascular events than females of...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2014.02.004
更新日期：2014-09-01 00:00:00
abstract：:Modification of GABAergic inhibition is an intensely investigated hypothesis guiding research into mechanisms underlying temporal lobe epilepsy (TLE). Seizures can be initiated by blocking γ amino butyric acid type A (GABAA receptors, GABARs), which mediate fast synaptic inhibition in the brain, and controlled by drug...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2011.10.028
更新日期：2013-06-01 00:00:00
abstract：:We have previously demonstrated that octanoic (OA) and decanoic acids (DA) inhibit Na+, K+ ATPase activity in synaptic plasma membranes from rat brain. The objective of the present study was to investigate the in vitro effects of the other metabolites that accumulate in tissues of medium-chain acyl-CoA dehydrogenase (...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2005.09.002
更新日期：2006-01-01 00:00:00
abstract：:Neglect is a clinical syndrome characterized by an inability to orient, report, or respond to novel or meaningful stimuli presented to one side of the body or in one hemispace. Neglect in humans and monkeys most often results from damage to one of several interconnected cortical areas, including limbic cortex. In rats...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(89)90132-5
更新日期：1989-09-01 00:00:00
abstract：:Hypernatremic dehydration was induced in rabbits during a 3- to 5-day period resulting in mean plasma sodium concentrations of 187 meq/liter. The animals were then rehydrated during a 4-h period by intravenous administration of a 2.5% glucose or fructose solution. The water content of four regions of brain sample show...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90215-8
更新日期：1985-02-01 00:00:00
abstract：:Previously injured dorsal roots were electrically stimulated to determine if regenerating sensory axons can form physiologically active synaptic contacts with neurons within fetal spinal cord tissue transplants. Dorsal rootlets, sectioned at their spinal cord entry zone, were apposed to intraspinal transplants of feta...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1996.0101
更新日期：1996-06-01 00:00:00
abstract：:Vasoactive intestinal peptide (VIP) is a regulator of rodent embryogenesis during the period of neural tube closure. VIP enhanced growth in whole cultured mouse embryos; treatment with a VIP antagonist during embryogenesis inhibited growth and development. VIP antagonist treatment during embryogenesis also had permane...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2007.04.004
更新日期：2007-07-01 00:00:00
abstract：:MK-801, a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, was tested for anticonvulsant effects in rats using two seizure models, coadministration of lithium and pilocarpine and administration of a high dose of pilocarpine alone. Three major results are reported. First, pretreatment with MK-801 produce...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(89)90091-5
更新日期：1989-11-01 00:00:00
abstract：:In this review we focus on Charcot-Marie-Tooth (CMT) neuropathies and hereditary spastic paraplegias (HSPs). Although these diseases differ in whether they primarily affect the peripheral or central nervous system, both are genetically determined, progressive, long axonopathies that affect motor and sensory pathways. ...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2012.01.010
更新日期：2013-08-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal peptide (VIP), and their receptors have been localized within the hippocampus but their physiological function on synaptic transmission is still unclear. We investigated the effects of PACAP and VIP on evoked excitatory postsynaptic curr...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/S0014-4886(03)00300-5
更新日期：2003-12-01 00:00:00
