Abstract:
:Long non-coding RNAs (lncRNAs) are involved in regulating chromatin modifications, gene transcription, mRNA translation, and protein function. We recently reported a high variation in the basal expression levels of a panel of lncRNAs in HeLa and MCF-7 cells and their differential response to DNA damage induction. Here, we hypothesized that lncRNA molecules with different cellular expression may have a differential abundance in secreted exosomes, and their exosome levels would reflect cellular response to DNA damage. MALAT1, HOTAIR, lincRNA-p21, GAS5, TUG1, CCND1-ncRNA in exosomes secreted from cultured cells were characterized. A different expression pattern of lncRNAs in exosomes was seen compared to cells. RNA molecules with relative low expression levels (lincRNA-p21, HOTAIR, ncRNA-CCND1) were highly enriched in exosomes. TUG1 and GAS5 levels were moderately elevated in exosomes, whereas MALAT1--which was the most abundant molecule in cells--was present at levels comparable to its cellular levels. lincRNA-p21 and ncRNA-CCND1 were the main molecules; exosome levels of them best reflect the change of their cellular levels upon exposure of the cells to bleomycin-induced DNA damage. In conclusion, we provide evidence that lncRNAs have a differential abundance in exosomes, indicating a selective loading.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Gezer U,Özgür E,Cetinkaya M,Isin M,Dalay Ndoi
10.1002/cbin.10301subject
Has Abstractpub_date
2014-09-01 00:00:00pages
1076-9issue
9eissn
1065-6995issn
1095-8355journal_volume
38pub_type
杂志文章abstract::Endothelial progenitor cells (EPCs) are precursor cells of vascular endothelial cells, which are widely involved in the pathological process of cardiovascular diseases. EPCs apoptosis could accelerate the process of cardiovascular diseases. 14-3-3-η protein has been proved to be a potent antiapoptosis molecule. Howeve...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11529
更新日期:2020-12-15 00:00:00
abstract::Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. In the pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1006
更新日期:1995-01-01 00:00:00
abstract::Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facin...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100927
更新日期:2011-10-01 00:00:00
abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10427
更新日期:2015-05-01 00:00:00
abstract::Stem cell biology has gained remarkable interest in recent years, driven by the hope of finding cures for numerous diseases including skin wound healing through transplantation medicine. Initially upon transplantation, these cells home to and differentiate within the injured tissue into specialised cells. Contrariwise...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10138
更新日期:2013-10-01 00:00:00
abstract::Hepatic oval cells are likely to be activated during advanced stage of liver fibrosis to reconstruct damaged hepatic tissue. However, their scarcity, difficulties in isolation, and in vitro expansion hampered their transplantation in fibrotic liver. This study was aimed to investigate the repair potential of in vitro ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10699
更新日期:2017-01-01 00:00:00
abstract::The mitogen activated protein kinases (MAPK) signaling cascade plays an important role in cell life. We proved that small interfering RNAs targeting MAPK1 (siRNA-2) could inhibit HeLa cell growth, but the effects of siRNA-2 on gene expression profile were unclear. Using Affymetrix GeneChip HG-U133A 2.0, we identified ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.019
更新日期:2008-09-01 00:00:00
abstract::The effect of piperine on the fertilization of eggs with sperm was investigated in female hamsters. They were intragastrically treated with piperine at doses of 50 and 100 mg/kg BW from day 1 through day 4 of the oestrous cycle. During piperine treatment, these females were superovulated and artificially inseminated (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0167
更新日期:1997-07-01 00:00:00
abstract::Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090063
更新日期:2010-02-22 00:00:00
abstract::Hypoxia-inducible factor-1α (HIF-1α) and hepatocyte growth factor (HGF) play important roles in postnatal neovascularization. However, the interaction of these two pathways is not fully understood. The present study utilized CoCl(2) treated-endothelial progenitor cells (EPCs) (EPCs exposure to CoCl(2) are under mimic ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10397
更新日期:2015-03-01 00:00:00
abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11469
更新日期:2021-01-01 00:00:00
abstract::As a bone-derived hormone, fibroblast growth factor-23 (FGF-23) negatively regulates phosphate and calcium metabolism, while retaining growth-promoting action for mesenchymal cell differentiation. Elevated FGF-23 levels, together with hyperparathyroidism, are often observed in chronic kidney disease, which is associat...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10573
更新日期:2016-03-01 00:00:00
abstract::Under the 1G condition, the increase in antipain-sensitive protease activity promptly after UV (mainly 254 nm wavelength) irradiation in cultured human cells is detected and found to be one of the intriguing events involved in suppression of cell mutability. It was found that two cell lines, RSa and its variant UVAP-1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00285-8
更新日期:2003-01-01 00:00:00
abstract::The synthesis of proteins from salt-tolerant Brassica oleracea L. var. botrytis L. subvar. cauliflora (Gars.) DC. (cauliflower) cell cultures is modified in relation to controls in several features. There are nine newly induced polypeptides in tolerant cultures (absent in control conditions). Some of them are only pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1146
更新日期:1993-09-01 00:00:00
abstract::Reactive oxygen species (ROS) are not only generated in conditions of cellular stress but are also constitutively produced in most cell types by specific metabolic processes. This research focused on a potential antioxidant Trolox (model compound for alpha-tocopherol), with the aim to establish exact mechanisms of Tro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.11.010
更新日期:2005-01-01 00:00:00
abstract::Stem/progenitor cells hold promise for alleviating/curing type 1 diabetes due to the capacity to differentiate into functional insulin-producing cells. The current study aims to assess the differentiation potential of human pancreatic IPCs (islet-derived progenitor cells). IPCs were derived from four human donors and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100152
更新日期:2011-05-01 00:00:00
abstract::Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morpho...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.03.012
更新日期:2004-01-01 00:00:00
abstract::The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10900
更新日期:2018-07-01 00:00:00
abstract::The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and ma...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100919
更新日期:2012-08-01 00:00:00
abstract::The effects of NO in its environment may vary considerably depending on various factors. This study shows oxidative mechanism of cellular membrane alterations, which is not associated with triggering of ONOOH generation but is induced by pure NO. Our investigation examined the influence of low concentration of NO (0.1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.020
更新日期:2008-01-01 00:00:00
abstract::Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.005
更新日期:2008-08-01 00:00:00
abstract::Fibrillin-1 is the major component of extracellular matrix microfibrils. Microfibrils dysfunction is responsible for the onset of various connective tissue diseases, including Marfan syndrome. Although ADAMTSL (a disintegrin and metalloproteinase with thrombospondin motifs-like) 6β is one of the fibrillin-1 binding pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11337
更新日期:2020-07-01 00:00:00
abstract::ROS (reactive oxygen species) take an important signalling role in angiogenesis. Although there are several ways to produce ROS in cells, multicomponent non-phagocytic NADPH oxidase is an important source of ROS that contribute to angiogenesis. In the present work, we examined the effects of H2O2 on angiogenesis inclu...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090092
更新日期:2010-10-01 00:00:00
abstract::The introduction of four key transcriptional factors (CRX, RAX, NEURO-D, OTX2) allows the direct differentiation of fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination of mono-cistronic viruses. Although the combination of mono-cistronic viruses was useful, a relatively high ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10942
更新日期:2018-05-01 00:00:00
abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1072
更新日期:1995-04-01 00:00:00
abstract::TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090005
更新日期:2010-07-01 00:00:00
abstract::Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and tr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.11.013
更新日期:2004-01-01 00:00:00
abstract::MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little was revealed about the function of miR-21 in retinoblastoma (RB). In this study, we examined the expression of miR-21 in RB tissues and explored the relationship between miR-21 and phosphatase and tensi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10678
更新日期:2016-12-01 00:00:00
abstract::We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0754
更新日期:2001-01-01 00:00:00
abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0343
更新日期:1999-01-01 00:00:00