Abstract:
:The usage of gelatin hydrogel is limited due to its instability and poor mechanical properties, especially under physiological conditions. Divalent metal ions present in gelatin such as Ca(2+) and Fe(2+) play important roles in the gelatin molecule interactions. The objective of this study was to determine the impact of divalent ion removal on the stability and mechanical properties of gelatin gels with and without chemical crosslinking. The gelatin solution was purified by Chelex resin to replace divalent metal ions with sodium ions. The gel was then chemically crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Results showed that the removal of divalent metal ions significantly impacted the formation of the gelatin network. The purified gelatin hydrogels had less interactions between gelatin molecules and form larger-pore network which enabled EDC to penetrate and crosslink the gel more efficiently. The crosslinked purified gels showed small swelling ratio, higher crosslinking density and dramatically increased storage and loss moduli. The removal of divalent ions is a simple yet effective method that can significantly improve the stability and strength of gelatin hydrogels. The in vitro cell culture demonstrated that the purified gelatin maintained its ability to support cell attachment and spreading.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Xing Q,Yates K,Vogt C,Qian Z,Frost MC,Zhao Fdoi
10.1038/srep04706subject
Has Abstractpub_date
2014-04-16 00:00:00pages
4706issn
2045-2322pii
srep04706journal_volume
4pub_type
杂志文章abstract::The lack of vaccines and antiviral treatment, along with the increasing number of cases of Zika virus (ZIKV) and Chikungunya virus (CHIKV) infections, emphasize the need for searching for new therapeutic strategies. In this context, the marine brown seaweed Canistrocarpus cervicornis has been proved to hold great anti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65357-7
更新日期:2020-05-19 00:00:00
abstract::Recent increases in family planning (FP) use have been reported among women of reproductive age in union (WRAU) in Senegal. However, trends have not been monitored among harder-to-reach groups (including adolescents, unmarried and rural poor women), key to understanding whether FP progress is equitable. We combined da...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41006
更新日期:2017-01-20 00:00:00
abstract::The patterns of life exhibited by large populations have been described and modeled both as a basic science exercise and for a range of applied goals such as reducing automotive congestion, improving disaster response, and even predicting the location of individuals. However, these studies have had limited access to c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02625
更新日期:2013-01-01 00:00:00
abstract::Bleeding risk with antiplatelet therapy is an increasing clinical challenge. However, the inter-individual variation in this risk is poorly understood. We assessed whether the level of plasma creatine kinase, the enzyme that utilizes ADP and phosphocreatine to rapidly regenerate ATP, may modulate bleeding risk through...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06551
更新日期:2014-10-09 00:00:00
abstract::Contemporary improvements in the type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system offer a convenient way for genome editing in zebrafish. However, the low efficiencies of genome editing and germline transmission require a time-intensive and laborious sc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34555
更新日期:2016-09-29 00:00:00
abstract::We have successfully grown centimeter-sized layered [Formula: see text] single crystals under high oxygen pressures of 120-150 bar by the floating zone technique. This enabled us to perform neutron scattering experiments where we observe close to quarter-integer magnetic peaks below [Formula: see text] that are accomp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74884-2
更新日期:2020-10-22 00:00:00
abstract::Adenosine-to-Inosine (A-to-I) RNA editing is the most prevalent post-transcriptional modification of RNA molecules. Researchers have attempted to find reliable RNA editing using next generation sequencing (NGS) data. However, most of these attempts suffered from a high rate of false positives, and they did not conside...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41294-y
更新日期:2019-03-25 00:00:00
abstract::In the last decades, AC powered atmospheric dielectric barrier discharges (DBDs) in air with a liquid electrode have been proposed as a promising plasma technology with versatile applicability in medicine, agriculture and water treatment. The fundamental features of the micro-discharge filaments that make up this type...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29189-w
更新日期:2018-07-19 00:00:00
abstract::Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary properties of silk and its amorph...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07502-3
更新日期:2017-08-07 00:00:00
abstract::The Na(3p) + H2(X1Σg+) → NaH(X1Σ+) + H(2S) reaction plays an important role in the field of diabatic reaction dynamics. A set of new diabatic potential energy surfaces (PESs) of the NaH2 system are structured, which include the diabatic coupling between the lowest two adiabatic states. The electronic structure calcula...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35987-z
更新日期:2018-12-19 00:00:00
abstract::Seed setting is an important trait that contributes to seed yield and relies greatly on starch accumulation. In this study, a sulfoquinovosyl transferase-like protein, designated as SQD2.2 involved in seed setting and flavonoid accumulation, was identified and characterized in rice. Rice SQD2.2 is localized to the cyt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04002-2
更新日期:2017-07-05 00:00:00
abstract::Faithful DNA replication is a prerequisite for cell proliferation. Several cytological studies have shown that chromosome structures alter in the S-phase of the cell cycle. However, the molecular mechanisms behind the alteration of chromosome structures associated with DNA replication have not been elucidated. Here, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12720
更新日期:2015-07-30 00:00:00
abstract::Salvia miltiorrhiza is one of the most widely-used medicinal plants. Here, we systematically analyzed the RNA editing events in its mitochondria. We developed a pipeline using REDItools to predict RNA editing events from stand-specific RNA-Seq data. The predictions were validated using reverse transcription, RT-PCR am...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42250
更新日期:2017-02-10 00:00:00
abstract::Nanopore-based sensing has emerged as a promising candidate for affordable and powerful DNA sequencing technologies. Herein, we demonstrate that nanopores can be successfully fabricated in Mg alloys via focused electron beam (e-beam) technology. Employing in situ high-resolution transmission electron microscopy techni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01920
更新日期:2013-01-01 00:00:00
abstract::Some studies have demonstrated interactions of AD-risk single nucleotide polymorphisms (SNPs) in non-APOE regions with APOE genotype. Nevertheless, no study reported interactions of expression quantitative trait locus (eQTL) for APOE with APOE genotype. In present study, we included 9286 unrelated AD patients and 8479...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26398-1
更新日期:2018-05-23 00:00:00
abstract::Globally, lung cancer accounts for approximately 20% of all cancer related deaths. Five-year survival is poor and rates have remained unchanged for the past four decades. There is an urgent need to identify markers of lung carcinogenesis and new targets for therapy. Given the recent successes of immune modulators in c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18907
更新日期:2016-01-13 00:00:00
abstract::It has been well established that some Armillaria species are symbionts of Polyporus umbellatus, However, little is known about the evolutionary history of P. umbellatus-Armillaria associations. In this research, we used an analysis based on the strength of the phylogenetic signal to investigate P. umbellatus-Armillar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04578-9
更新日期:2017-06-26 00:00:00
abstract::The impact of ctDNA changes after chemotherapy on the clinical outcomes of patients with metastatic colorectal cancer (mCRC) remains unclear. The present study evaluated the clinical implications of the early change in ctDNA levels as a predictor of objective response and clinical outcome in mCRC patients who received...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53711-3
更新日期:2019-11-22 00:00:00
abstract::Neural networks can efficiently encode the probability distribution of errors in an error correcting code. Moreover, these distributions can be conditioned on the syndromes of the corresponding errors. This paves a path forward for a decoder that employs a neural network to calculate the conditional distribution, then...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11266-1
更新日期:2017-09-08 00:00:00
abstract::Silver nanoparticles (AgNPs) have been extensively used and are considered as an emerging contaminant in the ocean. The environmental contamination of AgNPs is expected to increase greatly over time, and cetaceans, as the top ocean predators, will suffer the negative impacts of AgNPs. In the present study, we investig...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23737-0
更新日期:2018-04-04 00:00:00
abstract::The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42977
更新日期:2017-02-17 00:00:00
abstract::We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13289
更新日期:2015-08-21 00:00:00
abstract::Full-endoscopic transforaminal lumbar discectomy (FETD) is increasingly used in patients with lumbar disc herniation (LDH). There is little knowledge on the related factors, including the straight leg raising test (SLR), that influence the operation. Consecutive patients with LDH who came to our hospital from August 2...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73357-w
更新日期:2020-10-05 00:00:00
abstract::Nanofluids using nanoencapsulated Phase Change Materials (nePCM) allow increments in both the thermal conductivity and heat capacity of the base fluid. Incremented heat capacity is produced by the melting enthalpy of the nanoparticles core. In this work two important advances in this nanofluid type are proposed and ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17841-w
更新日期:2017-12-14 00:00:00
abstract::Alcoholic liver disease (ALD) causes significant morbidity and mortality, and pharmacological treatment options are limited. In this study, we evaluated the PCSK9 inhibitor alirocumab, a monoclonal antibody that robustly reduces low-density lipoprotein cholesterol (LDL-C), for the treatment of ALD using a rat model of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53603-6
更新日期:2019-11-20 00:00:00
abstract::We propose a novel method to achieve tunable plasmon focusing in graphene/photonic-crystal hybrid structure exhibiting all-angle negative refraction at terahertz frequencies. A two-dimensional photonic crystal composed of a square lattice of dielectric rods is constructed on the substrate of a graphene sheet to provid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41788
更新日期:2017-02-02 00:00:00
abstract::The microbial community during fermented vegetable production has a large impact on the quality of the final products. Lactic acid bacteria have been well-studied in such processes, but knowledge about the roles of non-lactic acid bacteria is limited. This study aimed to provide useful knowledge about the relationship...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81105-x
更新日期:2021-01-19 00:00:00
abstract::The association between choline uptake and androgen receptor (AR) expression is suggested by the upregulation of choline kinase-alpha in prostate cancer. Recently, detection of AR aberration in cell-free DNA as well as early 18F-fluorocholine positron emission tomography/computed tomography (FCH-PET/CT) were associate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15928-y
更新日期:2017-11-14 00:00:00
abstract::Cell counts decrease with sediment depth. Typical explanations consider limiting factors such as water availability and chemistry, carbon source, nutrients, energy and temperature, and overlook the role of pore size. Our analyses consider sediment self-compaction, the evolution of pore size with depth, and the probabi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78714-3
更新日期:2020-12-10 00:00:00
abstract::The ability to predict others' behaviour represents a crucial mechanism which allows individuals to react faster and more appropriately. To date, several studies have investigated humans' ability to predict conspecifics' behaviour, but little is known on our ability to predict behaviour in other species. Here, we aime...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78275-5
更新日期:2020-12-04 00:00:00