Function of DNA methyltransferase 3a in lead (Pb(2+) )-Induced Cyclooxygenase-2 gene.


:Lead ions (Pb(2+) ) are toxic industrial pollutants associated with chronic inflammatory diseases in humans and animals. Previously, we found that Pb(2+) ions induce COX-2 gene expression via the EGF receptor/nuclear factor-κB signal transduction pathway in epidermoid carcinoma cell line A431. In this study, to see whether Pb(2+) ions affect COX-2 expression by epigenetic mechanisms, we looked at the mRNAs of DNA methyltransferases (DNMTs) using real-time PCR of total RNA from these cells. Cells exposed to Pb(2+) had low levels of DNMT3a mRNA, whereas the levels of DNMT1 and DNMT3b mRNAs remained unchanged. Pretreatment of cells with DNMT inhibitor 5-aza-2'-deoxycytidine (5 μM) followed by Pb(2+) (1 μM) significantly increased levels of COX-2 mRNA compared with cells treated with Pb(2+) alone. Overexpression of tumor suppressor gene Rb correlated with an increase in COX-2 mRNA and a decrease in DNMT3a mRNA. Conversely, overexpression of transcription factor E2F1 correlated with a decrease in COX-2 mRNA and an increase in DMNT3a mRNA. Pretreatment with EGFR inhibitors AG1478 and PD153035 significantly limited Pb(2+) -induced reduction in DNMT3a mRNA. In addition, gene knockdown of DNMT3a with short hairpin RNA correlated with increased COX-2 mRNA induced by Pb(2+) . Our findings suggest Pb(2+) ions induce COX-2 expression indirectly by reducing DNMT3a methylation of the COX-2 promoter via transcription factors Rb and E2F1.


Environ Toxicol


Environmental toxicology


Tsai YT,Chang CM,Wang JY,Hou MF,Wang JM,Shiurba R,Chang WC,Chang WC




Has Abstract


2015-09-01 00:00:00












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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Vasseur P,Leguille C

    更新日期:2004-08-01 00:00:00

  • Acute toxicity of fatty acids to the freshwater green alga Selenastrum capricornutum.

    abstract::The acute toxicity of fatty acids (C14 to C18) commonly found in wood was determined by the standard algal growth inhibition test using the freshwater green alga Selenastrum capricornutum. Toxicity, quantified as IC50 values, varied depending on the number of total carbons and double bonds. Of the tested acids, oleic ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Kamaya Y,Kurogi Y,Suzuki K

    更新日期:2003-10-01 00:00:00

  • Mechanism of bisphenol AF-induced progesterone inhibition in human chorionic gonadotrophin-stimulated mouse Leydig tumor cell line (mLTC-1) cells.

    abstract::Bisphenol AF (BPAF) has been shown to inhibit testicular steroidogenesis in male rats. However, the precise mechanisms related to the toxic effects of BPAF on reproduction remain poorly understood. In the present study, a mouse Leydig tumor cell line (mLTC-1) was used as a model to investigate the mechanism of steroid...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Feng Y,Shi J,Jiao Z,Duan H,Shao B

    更新日期:2018-06-01 00:00:00

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    abstract::Laboratory toxicity tests were performed to obtain more data on the toxicity of ammonia to saltwater organisms. The standards for in-stream ammonia limits in marine environments presently are based on toxicity tests involving both freshwater and saltwater organisms. Acute tests (48 and 96 h) were performed at 20 degre...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Boardman GD,Starbuck SM,Hudgins DB,Li X,Kuhn DD

    更新日期:2004-04-01 00:00:00

  • Inflammatory activity in river-water samples.

    abstract::Contamination of the urban aquatic environment with chemical and biological substances could have a long-term impact on human health because these substances threaten the integrity of the urban ecosystem and the availability of high-quality water for recreation and consumption. In light of this, the aim of the present...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Wichmann G,Daegelmann C,Herbarth O,Strauch G,Schirmer K,Wöstemeyer J,Lehmann I

    更新日期:2004-12-01 00:00:00

  • Long noncoding RNA MACC1-AS1 promotes the stemness of nonsmall cell lung cancer cells through promoting UPF1-mediated destabilization of LATS1/2.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Wang X,Yu X,Wei W,Liu Y

    更新日期:2020-09-01 00:00:00

  • Nonylphenol regulates cyclooxygenase-2 expression via Ros-activated NF-κB pathway in sertoli TM4 cells.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Liu X,Nie S,Huang D,Xie M

    更新日期:2015-09-01 00:00:00

  • l-Carnitine protects against 1,4-benzoquinone-induced apoptosis and DNA damage by suppressing oxidative stress and promoting fatty acid oxidation in K562 cells.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Sun R,Man Z,Ji J,Ji S,Xu K,Pu Y,Yu L,Zhang J,Yin L,Pu Y

    更新日期:2020-10-01 00:00:00

  • Biomarker analyses in caged and wild fish suggest exposure to pollutants in an urban area with a landfill.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Hanson N,Larsson Å

    更新日期:2011-06-01 00:00:00

  • More serious autophagy can be induced by ZnO nanoparticles than single-walled carbon nanotubes in rat tracheal epithelial cells.

    abstract::Metal oxide nanoparticles and carbon nanoparticles, as common nanoparticles (NPs), can cause autophagy in certain cells, which will lead to biohealth risk issues. This study determined the difference in autophagy induced by zinc oxide nanoparticles (ZnO NPs) and single-walled carbon nanotubes (SWCNTs) in respiratory e...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Zhang H,Chen F,Li Y,Shan X,Yin L,Hao X,Zhong Y

    更新日期:2021-02-01 00:00:00

  • Inhibitory effect of GMI, an immunomodulatory protein from Ganoderma microsporum, on myofibroblast activity and proinflammatory cytokines in human fibrotic buccal mucosal fibroblasts.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Lee PH,Hsieh PL,Liao YW,Yu CC

    更新日期:2018-01-01 00:00:00

  • Differential effects of route of T-2 toxin exposure on hepatic oxidative damage in mice.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Chaudhary M,Bhaskar AS,Rao PV

    更新日期:2015-01-01 00:00:00

  • Genotoxic, cytotoxic, and apoptotic effects of Hypogymnia physodes (L.) Nyl. on breast cancer cells.

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    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Ari F,Celikler S,Oran S,Balikci N,Ozturk S,Ozel MZ,Ozyurt D,Ulukaya E

    更新日期:2014-05-01 00:00:00

  • Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans-nonachlor in rat primary hepatocytes.

    abstract::Recent epidemiological studies have revealed significant positive associations between exposure to organochlorine (OC) pesticides and occurrence of the metabolic syndrome and there are a growing number of animal-based studies to support causality. However, the cellular mechanisms linking OC compound exposure and metab...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Howell GE 3rd,McDevitt E,Henein L,Mulligan C,Young D

    更新日期:2018-09-01 00:00:00

  • Identification of toxicants from marine sediment using effect-directed analysis.

    abstract::Effect-directed analysis (EDA) has been reported to be a powerful tool for the identification of the responsible toxicants in contaminated, hazardous environmental samples. The aim of this study was to investigate whether it also is possible to use currently available EDA methodology to identify potentially relevant t...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Grote M,Brack W,Altenburger R

    更新日期:2005-10-01 00:00:00

  • Non destructive in vivo measurement of ethoxyresorufin biotransformation by zebrafish prolarva: development and application.

    abstract::Biotransformation enzymatic activities, such as the cytochrome P450 one, have been used as biomarkers for pollution assessment for a long time. Nevertheless, such biochemical tools are destructive processes, because they are performed on fish liver or total larvae homogenates. Moreover, the adaptation of this bioassay...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Noury P,Geffard O,Tutundjian R,Garric J

    更新日期:2006-08-01 00:00:00

  • Physiological responses of Microcystis aeruginosa PCC7806 to nonanoic acid stress.

    abstract::A recent study has shown that nonanoic acid (NA) is one of the strongest allelochemicals to a cyanobacterium Microcystis aeruginosa, but the physiological responses of M. aeruginosa to NA stress remain unknown. In this study, physiological characters such as the growth rate, photosynthetic processes, phosphorus and ni...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Shao JH,Wu XQ,Li RH

    更新日期:2009-12-01 00:00:00

  • Seasonal evaluation of reproductive status and exposure to environmental estrogens in hornyhead turbot at the municipal wastewater outfall of Orange County, CA.

    abstract::Seasonal changes in developmental stages, condition factor (CF), gonadosomatic index, and plasma vitellogenin (Vtg) concentrations in male and female hornyhead turbot were examined at the wastewater outfall (T1) of the Orange County Sanitation District, and two farfield sites T11 (7.7 km northwest of the outfall) and ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Deng X,Rempel MA,Armstrong J,Schlenk D

    更新日期:2007-10-01 00:00:00

  • Adrenomedullin reduces antioxidant defense system and enhances kidney tissue damage in cadmium and lead exposed rats.

    abstract::Adrenomedullin (AdM) is synthesized and secreted by a number of cells and tissue. AdM is a potent vasodilator but it is also considered a neuromodulator, an angiogenic factor, and a hormone regulator. AdM possess antiapoptotic, antioxidant, and antimicrobial properties. Heavy metals such as cadmium and lead are found ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Yurekli M,Esrefoglu M,Ilker Doğru M,Doğru A,Gul M,Whidden M

    更新日期:2009-06-01 00:00:00

  • Hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish following sub-chronic exposure to BDE-47 and BDE-153.

    abstract::Polybrominated diphenyl ethers (PBDEs) are ubiquitous and prolific contaminant in both the abiotic and biotic environment because of the wide industrial applications of these chemicals. In the present study, the effects of 2,2',4,4'-tetrabrominateddiphenyl ether (BDE-47) and 2,2',4,4',5,5'-hexabromodiphenyl ether (BDE...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Meng S,Chen X,Gyimah E,Xu H,Chen J

    更新日期:2020-11-01 00:00:00

  • Pesticide induced alterations in marrow physiology and depletion of stem and stromal progenitor population: an experimental model to study the toxic effects of pesticide.

    abstract::Long-term exposure of agriculturally used organochloride and organophosphate pesticides have been shown to cause long-lasting hematotoxicity and increased incidence of aplastic anemia in humans. The mechanisms involved in pesticide induced hematotoxicity and the features of toxicity that may play a major role in bone ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Chatterjee S,Basak P,Chaklader M,Das P,Pereira JA,Chaudhuri S,Law S

    更新日期:2014-01-01 00:00:00

  • Genotoxicity of dicrotophos, an organophosphorous pesticide, assessed with different assays in vitro.

    abstract::Dicrotophos is a systemic insecticide with a wide range of applications. We investigated the genotoxicity of dicrotophos using the Ames test, the chromosome aberration test in CHO-K1 cells, and the comet assay in the Hep G2 cells, while this chemicals' toxicity to both the cell lines was evaluated with the MTT assay. ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Wu JC,Chye SM,Shih MK,Chen CH,Yang HL,Chen SC

    更新日期:2012-05-01 00:00:00

  • Heavy metal-induced physiological alterations and oxidative stress in the moss Brachythecium piligerum chad.

    abstract::Antioxidative enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX)), as well as lipid peroxidation and proline were studied in moss Brachythecium piligerum Card. collected from different sites in Shanghai, China, to validate the physiological parameters as biomarkers of atm...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Sun SQ,He M,Wang GX,Cao T

    更新日期:2011-10-01 00:00:00

  • Assessment of mutagenicity and toxicity of different-size fractions of air particulates from La Plata, Argentina, and Leipzig, Germany.

    abstract::Airborne particulates, especially fine particles and bound chemical compounds, are a potential mediator of adverse health effects. In this study an analysis was done of the concentration and size distribution of air particulate matter, the content of bound polycyclic aromatic hydrocarbons (PAHs), and the biological ef...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Massolo L,Müller A,Tueros M,Rehwagen M,Franck U,Ronco A,Herbarth O

    更新日期:2002-01-01 00:00:00

  • Alteration of monoamine concentrations in the brain of medaka, Oryzias latipes, exposed to tributyltin.

    abstract::We measured the concentrations of monoamines in the brain of Japanese medaka, Oryzias latipes, exposed to tributyltin (TBT). Fish were exposed to 0, 1, 5, 25, or 125 microg g(-1) of TBT via the diet for 21 days. After the administration period, six males and six females in each treatment group were dissected and their...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Nakayama K,Oshima Y,Tachibana T,Furuse M,Honjo T

    更新日期:2007-02-01 00:00:00

  • Low-dose in utero exposure to finasteride promotes developmental changes in both male and female gerbil prostates.

    abstract::The prostate is an accessory reproductive gland that is sensitive to the action of exogenous compounds known as endocrine disrupters that alter normal hormonal function. Finasteride is a widely used chemical that acts to inhibit the conversion of testosterone in its most active form, dihydrotestosterone. It is known t...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Maldarine JS,Sanches BDA,Santos VA,Amaro GM,Calmon MF,Rahal P,Góes RM,Vilamaior PSL,Taboga SR

    更新日期:2020-01-01 00:00:00

  • Indium acetate toxicity in male reproductive system in rats.

    abstract::Indium, a rare earth metal characterized by high plasticity, corrosion resistance, and a low melting point, is widely used in the electronics industry, but has been reported to be an environmental pollutant and a health hazard. We designed a study to investigate the effects of subacute exposure of indium compounds on ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Lee KH,Chen HL,Leung CM,Chen HP,Hsu PC

    更新日期:2016-01-01 00:00:00

  • Secretion of interferon gamma from human immune cells is altered by exposure to tributyltin and dibutyltin.

    abstract::Tributyltin (TBT) and dibutyltin (DBT) are widespread environmental contaminants found in food, beverages, and human blood samples. Both of these butyltins (BTs) interfere with the ability of human natural killer (NK) cells to lyse target cells and alter secretion of the pro-inflammatory cytokine tumor necrosis factor...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Lawrence S,Reid J,Whalen M

    更新日期:2015-05-01 00:00:00

  • Hydrocarbon-induced changes to metabolic and detoxification enzymes of the Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis).

    abstract::The toxicity of petroleum hydrocarbons to marine aquatic organisms has been widely investigated; however, the effects on freshwater environments have largely been ignored. Selected biomarkers were measured in a freshwater species, the crimson-spotted rainbowfish (Melanotaenia fluviatilis). Fish were exposed to either ...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Pollino CA,Holdway DA

    更新日期:2003-02-01 00:00:00

  • Biochemical study on the protective effect of curcumin on acetaminophen and gamma-irradiation induced hepatic toxicity in rats.

    abstract::Acetaminophen (APAP) is one of the few recommended analgesic and antipyretic drugs in some critical cases such as viral disease COVID-19. However, the unrestricted use of APAP develops liver disorders. Hepatotoxicity and liver injury can also be induced by ionizing radiation (IR) during radiotherapy. The data of the c...

    journal_title:Environmental toxicology

    pub_type: 杂志文章


    authors: Eassawy MMT,Salem AA,Ismail AFM

    更新日期:2020-12-17 00:00:00