Abstract：
:Intracerebral hemorrhage (ICH) is a devastating form of stroke leading to a high rate of death and disability worldwide. Although it has been hypothesized that much of the IHC insult occurs in the subacute period mediated via a series of complex pathophysiological cascades, the molecular mechanisms involved in ICH have not been systematically characterized. Among the best approaches to understand the underlying mechanisms of injury and recovery, protein dynamics assessment via proteomics/systems biology platforms represent one of the cardinal techniques optimized for mechanisms investigation and biomarker identification. A proteomics approach may provide a biomarker focused framework from which to identify candidate biomarkers of pathophysiological processes involved in brain injury after stroke. In this work, a neuroproteomic approach (LC-MS/MS) was applied to investigate altered expression of proteins that are induced in brain tissue 3 h after injury in a rat model of ICH. Data from sham and focal ischemic models were also obtained and used for comparison. Based on the differentially expressed protein profile, systems biology analysis was conducted to identify associated cellular processes and related interaction maps. After LC-MS/MS analysis of the 3 h brain lysates, 86 proteins were differentially expressed between hemorrhagic and sham tissues. Furthermore, 38 proteins were differentially expressed between ischemic and sham tissues. On the level of global pathway analysis, hemorrhagic stroke proteins were shown to be involved in autophagy, ischemia, necrosis, apoptosis, calpain activation, and cytokine secretion. Moreover, ischemic stroke proteins were related to cell death, ischemia, inflammation, oxidative stress, caspase activation and apoptotic injury. In conclusion, the proteomic responses identified in this study provide key information about target proteins involved in specific pathological pathways.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Ren C,Guingab-Cagmat J,Kobeissy F,Zoltewicz S,Mondello S,Gao M,Hafeez A,Li N,Geng X,Larner SF,Anagli J,Hayes RL,Ji X,Ding Ydoi
10.1016/j.brainresbull.2014.02.005subject
Has Abstractpub_date
2014-03-01 00:00:00pages
46-56eissn
0361-9230issn
1873-2747pii
S0361-9230(14)00024-0journal_volume
102pub_type
杂志文章abstract：:The effects of chronic administration of delta 9-tetrahydrocannabinol (delta 9-THC) on the plasma and brain catecholamine (CA) levels were measured using high performance liquid chromatography-electrochemical detection (LC-EC) system. Intact male rats were injected daily with vehicle (50 microliter oil) or with delta ...
journal_title：Brain research bulletin
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1993-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1989-05-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 评论,杂志文章
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更新日期：2007-05-30 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2005-11-15 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(02)00867-5
更新日期：2002-11-30 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：1988-10-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2006-04-14 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90314-2
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(95)00049-k
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
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更新日期：1997-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
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更新日期：2016-09-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.11.005
更新日期：2017-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.11.020
更新日期：2019-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(01)00639-6
更新日期：2002-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90060-2
更新日期：1986-03-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90091-0
更新日期：1982-06-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(97)00022-1
更新日期：1997-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(87)90066-9
更新日期：1987-11-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2012.06.008
更新日期：2012-10-01 00:00:00
