当前位置： SCI文献检索 > Asian Pacific Journal of Tropical Medicine期刊下所有文献 > First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in South-eastern Iran.

First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in South-eastern Iran.

Abstract：

OBJECTIVE:To identify the Leishmania species in infected sand flies by Real-time PCR coupled with HRM analysis. METHODS:Real-time PCR coupled with HRM analysis targeting the first internal transcribed spacer (ITS1) of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish Leishmania species in sand flies specimens. RESULTS:Three out of 115 females of Phlebotomus sergenti (P. sergenti) (2.6%) were positive to Leishmania tropica (L. tropica). CONCLUSIONS:This is the first report on P. sergenti as the main and proven vector of anthroponitic cutaneous leishmaniasis in Dehbakri County using Real-time PCR coupled with HRM analysis. This method is rapid, sensitive and specific for diagnosing of parasites in infected Sand flies and ideal for large scale genotyping projects.

journal_name

Asian Pac J Trop Med

journal_title

Asian Pacific journal of tropical medicine

authors

Aghaei AA,Rassi Y,Sharifi I,Vatandoost H,Mollaie H,Oshaghi MA,Abai M,Rafizadeh S

doi

10.1016/S1995-7645(14)60002-X

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

93-6

issue

2

eissn

1995-7645

issn

2352-4146

pii

S1995-7645(14)60002-X

journal_volume

7

pub_type

杂志文章

相关文献

Asian Pacific Journal of Tropical Medicine文献大全
  • Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs.

    abstract：OBJECTIVE:To determine the contribution of total phenolic content (TPC) in glycation inhibitory activity of common tropical medicinal food and spices with potential antioxidative properties. METHODS:In vitro glucose-bovine serum albumin (BSA) assay was used. Ethanolic extracts of ten common household condiments/herbs ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60097-8

    authors： Ramkissoon JS,Mahomoodally MF,Ahmed N,Subratty AH

    更新日期：2013-07-01 00:00:00

  • Matrine inhibits bladder cancer cell growth and invasion in vitro through PI3K/AKT signaling pathway: An experimental study.

    abstract：OBJECTIVE:To study the inhibitory effect of matrine on bladder cancer cell growth and invasion in vitro through PI3K/AKT signaling pathway. METHODS:Human T24 bladder cancer cell lines were cultured and treated with different doses of matrine (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) as well as 20 μmol/L PI3K inhibitor LY2...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2017.05.009

    authors： Yang Y,Guo JX,Shao ZQ,Gao JP

    更新日期：2017-05-01 00:00:00

  • Correlation between IL-1β, IL-1Ra gene polymorphism and occurrence of polycystic ovary syndrome infertility.

    abstract：OBJECTIVE:To explore the relationship between IL-1β, IL-1Ra gene polymorphism and the occurrence of polycystic ovary syndrome (PCOS) infertility. METHODS:A total of 59 PCOS infertility cases visiting the reproductive center of our hospital from Mar. 2010 to Mar. 2012 and 56 healthy women were selected. ELISA method wa...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60030-9

    authors： Xia YH,Yao L,Zhang ZX

    更新日期：2013-03-01 00:00:00

  • Correlation of caveolin-1 expression with microlymphatic vessel density in colorectal adenocarcinoma tissues and its correlation with prognosis.

    abstract：OBJECTIVE:To study the expression of caveolin-1 in colorectal adenocarcinoma tissues and its correlation with microlymphatic vessel density (LMVD), and to investigate the clinical pathological prognostic significance of caveolin-1 and LMVD in patients with colorectal cancer. METHODS:The expression of caveolin-1 and LM...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2015.07.004

    authors： Xue J,Wu XL,Huang XT,Guo F,Yu HF,Zhang PC,Wang LK,Qu M,Pan LM

    更新日期：2015-08-01 00:00:00

  • Environmental characteristics of anopheline mosquito larval habitats in a malaria endemic area in Iran.

    abstract：OBJECTIVE:To determine the effects of environmental parameters of larval habitats on distribution and abundance of anopheline mosquitoes in Rudan county of Iran. METHODS:This cross-sectional study was conducted during the mosquito breeding season from February 2010 to October 2011. The anopheline larvae were collected...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60087-5

    authors： Soleimani-Ahmadi M,Vatandoost H,Hanafi-Bojd AA,Zare M,Safari R,Mojahedi A,Poorahmad-Garbandi F

    更新日期：2013-07-01 00:00:00

  • Evaluation of anti-tubercular activity of linolenic acid and conjugated-linoleic acid as effective inhibitors against Mycobacterium tuberculosis.

    abstract：OBJECTIVE:To evaluate a new pharmacological activity/effect of linolenic acid (α- and γ-form) and conjugated-linoleic acid (CLA) causing antibacterial activity against Mycobacterium tuberculosis (Mtb). METHODS:The anti-Mtb activity/effect of linolenic acid and CLA were determined using different anti-Mtb indicator met...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.01.021

    authors： Choi WH

    更新日期：2016-02-01 00:00:00

  • Clostridium difficile infection: a review of the literature.

    abstract：:Clostridium difficile is a Gram-positive, strictly anaerobic, spore-forming bacterium. It is the most common cause of antibiotic-associated diarrhea in hospitals and other healthcare facilities and is of significant concern because of the increasing morbidity and mortality rates as well as increased health care costs....

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60197-8

    authors： Khan FY,Elzouki AN

    更新日期：2014-09-01 00:00:00

  • Nephroprotective effect of Murraya koenigii on cyclophosphamide induced nephrotoxicity in rats.

    abstract：OBJECTIVE:To evaluate the nephroprotective effect of defatted methanolic extract and aqueous extract of Murraya koenigii (M. koenigii) against cyclophosphamide drug. METHODS:Nephrotoxicity was induced by cyclophosphamide in 7 days at 150 mg/kg body weight through intraperitoneal route in rat model. Nephroprotective ac...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2017.08.005

    authors： Mahipal P,Pawar RS

    更新日期：2017-08-01 00:00:00

  • Continued sustained release of VEGF by PLGA nanospheres modified BAMG stent for the anterior urethral reconstruction of rabbit.

    abstract：OBJECTIVE:To study the biocompatibility and neovascularization of the PLGA nanospheres wrapped with vascular endothelial growth factor (VEGF), which can improve bladder acellular matrix graft (BAMG) with local continuous release of VEGF. METHODS:A total of 18 rabbit model (length of stenosis: 3 cm) with anterior ureth...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60078-4

    authors： Wang JH,Xu YM,Fu Q,Song LJ,Li C,Zhang Q,Xie MK

    更新日期：2013-06-01 00:00:00

  • Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect.

    abstract：OBJECTIVE:To study the effect of levocarnitine (L-CN) on tissue inhibitor of metalloproteinase-1 (TIMP-1) and intercellular adhesion molecule-1 (ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells. METHODS:High-fat diet feeding and intraperitoneal injec...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.01.025

    authors： Wang SX,Tan L,Wang J,Zhong JQ

    更新日期：2016-03-01 00:00:00

  • Screening, phylogenetic analysis and antibiotic sensitivity pattern of Salmonella enterica serovar Typhi isolates from typhoid asymptomatic carriers.

    abstract：OBJECTIVE:To isolate the Salmonella enterica serovar Typhi (S. typhi) from asymptomatic typhoid carriers in the local population. To assess the antibiotic sensitivity and resistant pattern of S. typhi isolates against viable antibiotics and phylogenetic analysis of S. typhi isolates on the basis of 16S rDNA gene. METH...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60191-0

    authors： Chandrasekaran B,Balakrishnan S

    更新日期：2011-10-01 00:00:00

  • Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.).

    abstract：OBJECTIVE:To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). METHODS:Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiaz...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2017.06.004

    authors： Minarni,Artika IM,Julistiono H,Bermawie N,Riyanti EI,Hasim,Hasan AEZ

    更新日期：2017-06-01 00:00:00

  • Conserved regions of Plasmodium vivax potential vaccine candidate antigens in Sri Lanka: conscious in silico analysis of prospective conformational epitope regions.

    abstract：OBJECTIVES:To do mapping and modeling of conformational B cell epitope regions of highly conserved and protective regions of three merozoitecandidate vaccine proteins of Plasmodium vivax (P. vivax), ie. merozoite purface protein-1 (PvMSP-1), apical membrane antigen -1 domain ∏ (PvAMA1-D∏) and region ∏ of the Duffy bind...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60146-2

    authors： Amarasinghe S,Kathriarachchi H,Udagama P

    更新日期：2014-10-01 00:00:00

  • Anti-hyperglycemic activity of leaves extract of Hyptis suaveolens L. Poit in streptozotocin induced diabetic rats.

    abstract：OBJECTIVE:To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. METHODS:Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. RESULTS:A significant reduction in blood glucose was...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60175-2

    authors： Mishra SB,Verma A,Mukerjee A,Vijayakumar M

    更新日期：2011-09-01 00:00:00

  • Prevalence of shiga toxins (stx1, stx2), eaeA and hly genes of Escherichia coli O157:H7 strains among children with acute gastroenteritis in southern of Iran.

    abstract：OBJECTIVE:To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht. METHODS:In this study faecal sample from 615 children aged <5 years old who were hospitalized for gastroenteritis in Fars hospitals in Iran were collected and then enriched in Escherichia coli (E....

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60182-6

    authors： Kargar M,Homayoon M

    更新日期：2015-01-01 00:00:00

  • Preventive and therapeutic effect of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.

    abstract：OBJECTIVE:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage. METHODS:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group, model group and simvastatin-treated group randomly with 20 rats in each gro...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2017.01.003

    authors： Zhou HX,Gao LH,Meng LL,Zhang YX,Wei ZF,Si DW

    更新日期：2017-02-01 00:00:00

  • Imported cases of dengue fever in Russia during 2010-2013.

    abstract：OBJECTIVE:To confirm dengue infection among Russian tourists returned from Southeast and Mexico in 2010-2013 with clinical signs of infection. METHODS:Blood and serum samples from patients were collected. NS1 antigen and human IgM/IgG antibodies to dengue virus were identified using commercial tests manufactured by "S...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60194-2

    authors： Sergeeva EI,Ternovoi VA,Chausov EV,Berillo SA,Demina OK,Shikov AN,Plasunova IV,Kartashov MJ,Agafonov AP

    更新日期：2015-02-01 00:00:00

  • Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae).

    abstract：OBJECTIVE:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem. METHODS:During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats,...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60020-6

    authors： Kumar G,Karthik L,Rao KV

    更新日期：2013-03-01 00:00:00

  • Immunoprophylactic potential of filarial glutathione-s-transferase in lymphatic filariaisis.

    abstract：OBJECTIVE:To elucidates the immunoprophylactic potential of glutathion-s-transferase (GST) from cattle filarial parasite Setaria digitata (S. digitata) against lymphatic filariasis. METHODS:GST was purified through affinity chromatography (SdGST) and chacterized by SDS-PAGE and Nano-LC MS/MS analysis. Antibody isotype...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60066-7

    authors： Bal M,Mandal N,Achary KG,Das MK,Kar SK

    更新日期：2011-03-01 00:00:00

  • Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- κ B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis.

    abstract：OBJECTIVE:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model. METHODS:Experimental colitis was induced by dextran sulfate sodium (DSS), and mice were divided into 4 groups: control, DSS alone, DSS plus ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60306-0

    authors： Miao XP,Sun XN,Cui LJ,Cao QF,Zhuang GF,Deng TZ,Zhang DY

    更新日期：2015-02-01 00:00:00

  • Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus.

    abstract：OBJECTIVE:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion. METHODS:Two mentioned antigens were cloned and expressed in expression vector and purified by affi...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(12)60058-3

    authors： Abdi J,Kazemi B,Karimfar MH,Rokni MB

    更新日期：2012-05-01 00:00:00

  • Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings.

    abstract：OBJECTIVE:To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases. METHODS:Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct mic...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.07.018

    authors： Coskun A,Malatyali E,Ertabaklar H,Yasar MB,Karaoglu AO,Ertug S

    更新日期：2016-09-01 00:00:00

  • Acalculous cholecystitis: a rare presentation of leptospirosis progressing to Weil's disease.

    abstract：:Leptospirosis is a zoonotic infection with higher incidence in tropics. Leptospirosis, is known for its variable manifestations, and is a clinical challenge for physicians in the tropics. Experienced clinicians, at times can mistake leptospirosis for non-medical conditions. A few reports of leptospirosis presenting as...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60235-6

    authors： Peter G,Narasimha H

    更新日期：2011-12-01 00:00:00

  • Development of quality control parameters for the standardization of Limonia acidissima L. leaf and stem.

    abstract：OBJECTIVE:To evaluate the pharmacognostic characters of Limonia acidissima L. (L. acidissima) leaf and stem, an important traditional medicinal plant. METHODS:The present study provides pharmacognostic, physicochemical and phytochemical details of leaf and stem of L. acidissima. Micro and macroscopic characters were a...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60240-6

    authors： Pandavadra M,Chanda S

    更新日期：2014-09-01 00:00:00

  • Cytoprotective, antihyperglycemic and phytochemical properties of Cocos nucifera (L.) inflorescence.

    abstract：OBJECTIVE:To analyze the cytoprotective and antidiabetic activities as well as phytochemical composition of the immature inflorescence of Cocos nucifera belonging to the Arecaceae Family. METHODS:The phytochemical screening of inflorescence was done to determine the major constituents present in Cocos nucifera inflore...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60142-X

    authors： Renjith RS,Chikku AM,Rajamohan T

    更新日期：2013-10-01 00:00:00

  • Green coffee bean extract improves obesity by decreasing body fat in high-fat diet-induced obese mice.

    abstract：OBJECTIVES:To evaluate possible lipid catabolism and body fat regulation effects of 3-caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-induced obese mice. METHODS:Obesity was induced in mice using a HFD for four weeks. Then, mice were fed only HFD or HFD with GCBE at 50, 100, and 200 mg/k...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.05.017

    authors： Choi BK,Park SB,Lee DR,Lee HJ,Jin YY,Yang SH,Suh JW

    更新日期：2016-07-01 00:00:00

  • Ultrastructural observation on pulmonary fibrosis in E9 rats treated with compound Carapax trionycis formula.

    abstract：OBJECTIVE:To investigate ultrastructural changes in pulmonary tissue of a rat model of pulmonary fibrosis following treatment with compound Carapax trionycis (C. trionycis; Biejia in Chinese) formula. METHODS:Sixty male Sprague-Dawley rats were randomly divided into four groups (n=15): compound C. trionycis formula hi...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60013-9

    authors： Ren J,Wang LX,Ji XC,Zhou JY,Zhang MY

    更新日期：2013-02-01 00:00:00

  • Seasonal variations in health indices of free-ranging asymptomatic guinea fowls (Numida meleagris) in Zambia.

    abstract：OBJECTIVE:To determine the impact of seasonal variations on health indices of free-ranging asymptomatic guinea fowls (Numida meleagris) in Zambia. METHODS:A time series analysis was carried out on a prospective cohort study over a 12 month period between March 2010 and February 2011 by examining a total 147 guinea fow...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60221-2

    authors： Nalubamba KS,Mudenda NB,Bwalya EC,Masuku M,Munyeme M,Munang'andu HM

    更新日期：2014-09-01 00:00:00

  • Tropical pyomyositis presenting as sepsis with acute respiratory distress syndrome.

    abstract：:Tropical pyomyositis is an underdiagnosed condition. We reported a 35 year old male farmer, who presented with septicemia and acute respiratory distress syndrome due to pyomyositis involving the paraspinal muscles. Culture of the pus grew methicillin sensitive Staphylococcus aureus, and the patient recovered after sur...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60096-5

    authors： Siddalingana GT,Hande HM,Stanley W,Bargur R

    更新日期：2011-04-01 00:00:00

  • The role of heme-oxygenase-1 in pathogenesis of cerebral malaria in the co-culture model of human brain microvascular endothelial cell and ITG Plasmodium falciparum-infected red blood cells.

    abstract：OBJECTIVE:To investigate the role of human host heme-oxygenase-1 (HO-1) in pathogenesis of cerebral malaria in the in vitro model. METHODS:The effect of human host HO-1 [human brain microvascular endothelial cell (HBMEC)] on hemoglobin degradation in the co-culture model of HBMEC and ITG Plasmodium falciparum-infected...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.11.011

    authors： Thongdee P,Na-Bangchang K

    更新日期：2017-01-01 00:00:00