Abstract：
OBJECTIVE:To study the inhibitory effect of matrine on bladder cancer cell growth and invasion in vitro through PI3K/AKT signaling pathway. METHODS:Human T24 bladder cancer cell lines were cultured and treated with different doses of matrine (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) as well as 20 μmol/L PI3K inhibitor LY294002 for 24 h, and the cell proliferation activity, the number of invasive cells as well as the expression of p-PI3K, p-AKT, proliferation genes and invasion genes were determined. RESULTS:Different doses of matrine could decrease the cell viability value, the number of invasive cells as well as the expression of p-PI3K, p-AKT, MMP2 and MMP9, and increase the expression of p16, p21 and p27 in dose-dependent manner; p16, p21 and p27 expression in cells of 20 μmol/L LY29002 group were significantly higher than those of 0 μmol/L LY29002 group while MMP2 and MMP9 expression were significantly lower than those of 0 μmol/L LY29002 group (P < 0.05). CONCLUSIONS:Matrine can inhibit bladder cancer cell proliferation and invasion in vitro and regulate the expression of cell cycle-inhibiting molecules and invasion-related genes through PI3K/AKT signaling pathway.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Yang Y,Guo JX,Shao ZQ,Gao JPdoi
10.1016/j.apjtm.2017.05.009subject
Has Abstractpub_date
2017-05-01 00:00:00pages
515-519issue
5eissn
1995-7645issn
2352-4146pii
S1995-7645(17)30499-6journal_volume
10pub_type
杂志文章abstract：OBJECTIVE:To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR. METHODS:In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60087-0
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:To determine the effects of environmental parameters of larval habitats on distribution and abundance of anopheline mosquitoes in Rudan county of Iran. METHODS:This cross-sectional study was conducted during the mosquito breeding season from February 2010 to October 2011. The anopheline larvae were collected...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60087-5
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:To evaluate the curative effect of the 132 KD protein isolated from the seeds of Peganum harmala (P. harmala) L. against carbon tetrachloride (CCl(4)) induced oxidative stress in rats. METHODS:Animals were post treated intraperitoneally with 132 KD isolated protein at doses of 4 and 8 mg/kg body weight and b...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60058-9
更新日期：2013-04-13 00:00:00
abstract：OBJECTIVE:To describe the profile and geographical distribution of reported cases of cutaneous leishmaniasis (CL) in the province of Hail, Saudi Arabia from 2010 to 2013. METHODS:Human data were collected from the dermatology service of both King Khaled and General Hail hospitals. RESULTS:A total of 483 confirmed CL ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60332-1
更新日期：2015-04-01 00:00:00
abstract：OBJECTIVE:To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450 (CYP), i.e., CYP1A2, CYP2C19, and CYP3A4 using human liver microsomes in vitro. METHODS:Inhibitory effects of plumbagin on the three human CYP isoforms were investigated using pooled human liver microsomes. Phen...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.10.016
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). METHODS:Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiaz...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.06.004
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVE:To induce pluripotent stem (IPS) cells from fibrocytes that are separated from liver cancer patients. METHODS:The fibrocytes were reprogrammed to IPS cells by lentiviral vector, stained and identified by immunohistochemistry. RESULTS:The IPS cells were successfully established from fibrocytes after infectio...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60032-8
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis (OA) patients. METHODS:Knee synovial tissue were collected in 90 OA patients (the OA group). Patients in the OA group was divided into 3 subgroups: grade I subgroup (n=30), grade II subgroup...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60042-0
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl3 and ethanol (EtOH)] of Ternstroemia sylvatica Schltdl. & Cham. METHODS:The antioxidant potential was evaluated by ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.10.007
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:To study the correlation of lipid ratios and individual lipid indexes of patients with type 2 diabetes with glycosylated hemoglobin (HbA(1c)). METHODS:Samples were collected from 128 type 2 diabetic patients (aged 19-90 years; male 72, female 56). The sera were analyzed for HbA(1c), total cholesterol (TC), t...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60080-7
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:To study the effects of aqueous extract of Gmelina arborea bark on normoglycemic levels and streptozotocin (STZ) induced diabetes in rats. METHODS:After single administration of the aqueous extract, plasma glucose level was determined up to 6 h. In subacute study, the aqueous extract was administered for 28 ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60104-2
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:To estimate the genetic diversity of Kokobera virus, the date of origin and the spread among different viruses in the endemic regions of Australia. METHODS:Two datasets were built. The first consisting of 29 sequences of the NS5/3' UTR region of Kokobera group downloaded from GenBank, the second including on...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.07.028
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:To investigate the antidiabetic effect of aqueous extract of Merremia tridentata (M. tridentata) root (MTRAE) in normal, glucose-loaded hyperglycemic and streptozotocin (STZ)-induced diabetic rats. METHODS:Oral administration of MTRAE at the doses of 50, 100 and 150 mg/kg was studied in normal, glucose-loade...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60020-0
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:To explore the regulation effect of Rheum palmatum (R. palmatum) L. on NF-κB signaling pathway of ALF mice. METHODS:The intraperitoneal injection of d-GalN/LPS was employed for the model building. Mice in the treatment group and positive control group were given the R. palmatum L. and bifendate before the mo...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.09.011
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:To explore the effect and mechanism of angiotensin II receptor blockers - Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. METHODS:Rats with embryonic cardiomyocytes-H9c2 were randomly divided into control group, ischemia group, Irbesartan group and Irbesartan + ischemia ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.07.022
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:To study the effect of levocarnitine (L-CN) on tissue inhibitor of metalloproteinase-1 (TIMP-1) and intercellular adhesion molecule-1 (ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells. METHODS:High-fat diet feeding and intraperitoneal injec...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.01.025
更新日期：2016-03-01 00:00:00
abstract：OBJECTIVE:To determine the phytochemical composition, antioxidant and anticancer activities of ethanolic and water leaves extracts of Annona muricata (A. muricata) from the Eastern Uganda. METHODS:Phytochemical screening was conducted using standard qualitative methods and a Chi-square goodness of fit test was used to...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60258-3
更新日期：2014-09-01 00:00:00
abstract：:A natural prodrug is a chemical compound or substance obtained from plants, microorganism, animal and marine sources. Natural products are small molecule source for Food and Drug Administration approved drugs and major sources for drug discovery. Most of the drugs for different ailment diseases undergo first pass meta...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60203-0
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To delineate the frequency of occurrence of bla(CTX-M), bla(TEM), and bla(SHV) in Enterobacteriaceae from North-Indian tertiary hospital. METHODS:A random collection of a subset of 45 Escherichia coli (E. coli) and 28 Klebsiella pneumoniae (K. pneumoniae) that was resistant to a third generation cephalospori...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60046-1
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of the anthocyanin-rich extract of Hibiscus sabdariffa (H. sabdariffa) calyx on the viability of cadmium-treated U937 cells and cadmium-mediated activation of U937-derived macrophages. METHODS:The macrophage cell line U937 was treated with cadmium (0.1 μ mol/L) and later incubated w...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60241-1
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVES:To analyze the effects of feeding Helianthus tuberosus (HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet (HFD). METHODS:A normal HFD or HFD including 10 w/w% HT tubers (HFD + HT) was fed to F334/Jcl rats. After 10 weeks, organ weights, glucose tolerance, and lipid profile were a...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.03.028
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:To explore the larvicidal and pupicidal activity of Euphorbia hirta (E. hirta) leaf extract and Bacillus sphaericus (B. sphaericus) against the malarial vector, Anopheles stephensi (An. stephensi). METHODS:The larvicidal and pupicidal activity was assayed against An. stephensi at various concentrations rangi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60003-6
更新日期：2013-02-01 00:00:00
abstract：:Tuberculosis (TB) is a symbolic menace to mankind, infecting almost one third of the world's populace and causing over a million mortalities annually. Mycobacterium tuberculosis (Mtb) is the key pathogen of TB that invades and replicates inside the host's macrophage. With the emerging dilemma of multi-drug resistant t...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章,评审
doi：10.1016/j.apjtm.2016.09.005
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVE:To study the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in gastric cancer tissue and its correlation with malignant biology. METHODS:Gastric cancer tissue and adjacent normal tissue were collected, and mRNA content and protein content of TRAP1 were detected; gastric cancer cell...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.12.014
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:To develop attenuated strains of Salmonella enterica serovar Typhi (S. typhi) for the candidate vaccine by osmolar stress. METHODS:S. typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Namakkal, Tamil Nadu, India. Both strains were grown in LB (Luria Bertani) medium supplemented wi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60165-6
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To confirm dengue infection among Russian tourists returned from Southeast and Mexico in 2010-2013 with clinical signs of infection. METHODS:Blood and serum samples from patients were collected. NS1 antigen and human IgM/IgG antibodies to dengue virus were identified using commercial tests manufactured by "S...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60194-2
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To explore decreased proliferation ability and differentiation potential of mesenchymal stem cells (MSCs) of osteoporosis rat. METHODS:MSCs were obtained from osteoporosis rat, and proliferation potency and impaired osteogenic differentiation potential were determined. RESULTS:The result showed a significan...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60055-9
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVE:To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development. METHODS:Brucella melitensis (B. melitensis) 16M strain was cultured and bacterial DNA was extracted by Bioneer AccuPrep® Genomic DNA Extraction...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60089-3
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of exogenous recombinant human brain natriuretic peptide (rhBNP) after primary percutaneous coronary intervention (PCI) on non-invasive hemodynamic in acute myocardial infarction patients with left ventricular failure. METHODS:A number of 96 acute myocardial infarction patients acc...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.06.006
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:To observe the effect of recombinant human bone morphogenetic protein 2/poly-lactide-co-glycolic acid (rhBMP-2/PLGA) with core decompression on repair of rabbit femoral head necrosis. METHOD:Bilateral femoral head necrosis models of rabbit were established by steroid injection. A total of 48 rabbits (96 femo...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60156-5
更新日期：2014-11-01 00:00:00
