Abstract：
OBJECTIVE:To induce pluripotent stem (IPS) cells from fibrocytes that are separated from liver cancer patients. METHODS:The fibrocytes were reprogrammed to IPS cells by lentiviral vector, stained and identified by immunohistochemistry. RESULTS:The IPS cells were successfully established from fibrocytes after infection, and IPS cell clones formed in round shape under a microscopy. The induction rate was 0.013%±0.007%. No tumor formed at the back of nude mice within 8 weeks after the inoculation of cell clones. However, tetatoma appeared in nude mice within 1 week after IPS inoculation. A few tumors formed in nude mice within 4 weeks after the inoculation of cell clones. However, subcutaneous tumors formed within 1 week after IPS inoculation. The induced IPS cells showed three germ layers in tetatoma. Nanog and OCT4 in the induced IPS cells showed hypomethylation. SSEA-A, TRA-1-6-, TRA-1-81 and Nanog were highly expressed in the induced IPS cells, indicating the IPS cells possessed the similar ability as the stem cells. CONCLUSIONS:The IPS cells of liver cancer patients can be established effectively from fibrocytes and can be cultured stably in vitro, which provides an approach for the treatment of intermediate or advanced stage liver cancer.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Zhang DM,Li JJ,Yan P,Hu JTdoi
10.1016/S1995-7645(14)60032-8subject
Has Abstractpub_date
2014-04-01 00:00:00pages
253-6issue
4eissn
1995-7645issn
2352-4146pii
S1995-7645(14)60032-8journal_volume
7pub_type
杂志文章abstract：OBJECTIVE:To explore the larvicidal and pupicidal activity of Euphorbia hirta (E. hirta) leaf extract and Bacillus sphaericus (B. sphaericus) against the malarial vector, Anopheles stephensi (An. stephensi). METHODS:The larvicidal and pupicidal activity was assayed against An. stephensi at various concentrations rangi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60003-6
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:To observe the effect of recombinant human bone morphogenetic protein 2/poly-lactide-co-glycolic acid (rhBMP-2/PLGA) with core decompression on repair of rabbit femoral head necrosis. METHOD:Bilateral femoral head necrosis models of rabbit were established by steroid injection. A total of 48 rabbits (96 femo...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60156-5
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:To establish the prevalence and associated risk factors of Schistosoma mansoni (S. mansoni) infection among schoolchildren at a village in Wolaita Zone, Southern Ethiopia. METHODS:A cross-sectional study was carried out among primary schoolchildren. A total of 384 randomly selected study subjects provided st...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.05.009
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:The present study deals with the investigation of larvicidal and ovicidal activities of benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of Eclipta alba (E. alba) against dengue vector, Aedes aegypti (Ae. Aegypti). METHODS:Twenty five early III instar larvae of Ae. aegypti was exposed to ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60026-6
更新日期：2011-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the anti-inflammatory, analgesic, antioxidant and acute toxicity of extracts obtained from a successive extraction with solvents of ascending polarity [hexane, hex; chloroform, CHCl3 and ethanol (EtOH)] of Ternstroemia sylvatica Schltdl. & Cham. METHODS:The antioxidant potential was evaluated by ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.10.007
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:To isolate the Salmonella enterica serovar Typhi (S. typhi) from asymptomatic typhoid carriers in the local population. To assess the antibiotic sensitivity and resistant pattern of S. typhi isolates against viable antibiotics and phylogenetic analysis of S. typhi isolates on the basis of 16S rDNA gene. METH...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60191-0
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVE:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion. METHODS:Two mentioned antigens were cloned and expressed in expression vector and purified by affi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60058-3
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage. METHODS:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group, model group and simvastatin-treated group randomly with 20 rats in each gro...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2017.01.003
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE:To build the rat model of gastric precancerous lesions and discuss the effect of transplantation of mesenchymal stem cells (BMMSCs) on the pathological change. METHODS:The rat model of gastric precancerous lesions was built using N-methyl-N'-nitro-N'-nitrosoguanidine. After the intravenous transplantation of...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章,撤回出版物
doi：10.1016/j.apjtm.2015.11.006
更新日期：2015-12-01 00:00:00
abstract：OBJECTIVE:To investigate the efficiency of β-galactosidase gene transfer into rat kidney with ultrasound-mediated microbubble destruction via different injection routes. METHODS:A total of 25 Wistar rats were randomly divided into 5 groups. Four groups received a mixture of optison microbubbles (0.2 mL) and lacz plasm...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60099-6
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:To investigate antidiabetic, antihyperlipidemic and antioxidant activity of methanol extract of Tectona grandis (T. grandis) flowers (METGF) in streptozotocin (STZ) induced diabetic rats to supports its traditional use. METHODS:Acute toxicity study of METGF was carried out in rat to determine its dose for th...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60160-0
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To analyze the protective efficacy of recombinant 78 kDa antigen of Leishmania donovani in combination with two adjuvants, that is, cationic liposomes or MPL-A against visceral leishmaniasis in BALB/c mice. METHODS:The genomic DNA of promastigotes was isolated and 583 bp of T cell epitopes of gene encoding 7...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2015.06.008
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus furetianus by using rat model of atherosclerosis. METHODS:The air-dried powdered Mallotus furetianus leaves were extracted with ethanol and then evaporated. The ethanol extract was experienced Diaion HP-20 CC wi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60212-5
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVE:To develop attenuated strains of Salmonella enterica serovar Typhi (S. typhi) for the candidate vaccine by osmolar stress. METHODS:S. typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Namakkal, Tamil Nadu, India. Both strains were grown in LB (Luria Bertani) medium supplemented wi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60165-6
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:To provide further evidence for the ethnomedicinal use of the Eastern Nigeria mistletoe, Loranthus micranthus (L. micranthus), as an immunostimulant. METHODS:Solvent fractions from the crude extract of the mistletoe plant was obtained and screened by the cell mediated delayed type hypersensitivity reaction (...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60137-5
更新日期：2011-07-01 00:00:00
abstract：:Clostridium difficile is a Gram-positive, strictly anaerobic, spore-forming bacterium. It is the most common cause of antibiotic-associated diarrhea in hospitals and other healthcare facilities and is of significant concern because of the increasing morbidity and mortality rates as well as increased health care costs....
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60197-8
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Schotia latifolia (S. latifolia) bark commonly used in South Africa traditional medicine for the treatment of various ailments. METHODS:The antibacterial test and MIC was determined by using agar well diffusion and dilut...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(11)60225-3
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To investigate ultrastructural changes in pulmonary tissue of a rat model of pulmonary fibrosis following treatment with compound Carapax trionycis (C. trionycis; Biejia in Chinese) formula. METHODS:Sixty male Sprague-Dawley rats were randomly divided into four groups (n=15): compound C. trionycis formula hi...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60013-9
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:To study the effects of aqueous extract of Gmelina arborea bark on normoglycemic levels and streptozotocin (STZ) induced diabetes in rats. METHODS:After single administration of the aqueous extract, plasma glucose level was determined up to 6 h. In subacute study, the aqueous extract was administered for 28 ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60104-2
更新日期：2013-08-01 00:00:00
abstract：:Tuberculosis (TB) is a symbolic menace to mankind, infecting almost one third of the world's populace and causing over a million mortalities annually. Mycobacterium tuberculosis (Mtb) is the key pathogen of TB that invades and replicates inside the host's macrophage. With the emerging dilemma of multi-drug resistant t...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章,评审
doi：10.1016/j.apjtm.2016.09.005
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVE:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics, liver function, change of the serum inflammatory factors and cell apoptosis factors. METHODS:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow chang...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.05.009
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:To examine the acanthamoebicidal effects of ethyl acetate, aqueous and butanol fractions of dried flower buds of Lonicera japonica (L. japonica) Thunb. (Flos Lonicerae) in vitro. METHODS:Acanthamoeba triangularis isolates were obtained from environmental water samples and identified by PCR. They were exposed...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.07.008
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the antioxidant activity of aqueous extract of Moringa oleifera (M. oleifera) young leaves by in vivo as well as in vitro assays. METHODS:In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60068-1
更新日期：2013-06-01 00:00:00
abstract：:Hookworm infection is a relatively common cause of anemia in endemic areas. The most common hookworm species are Ancylostoma duodenale and Necator americanus. In this report we present a case of overt gastrointestinal bleeding because of hookworm infection. Capsule endoscopy revealed many hookworms in the lumen of pro...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(12)60051-0
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:To determine resistance status of Culex species to different class of insecticides and assess the major anti-mosquito strategies employed by urban households and their possible effects on malaria acquisition. METHODS:Structured questionnaires were randomly administered to obtain information on demographic ch...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60153-4
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the pharmacognostic characters of Limonia acidissima L. (L. acidissima) leaf and stem, an important traditional medicinal plant. METHODS:The present study provides pharmacognostic, physicochemical and phytochemical details of leaf and stem of L. acidissima. Micro and macroscopic characters were a...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60240-6
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To determine the contribution of total phenolic content (TPC) in glycation inhibitory activity of common tropical medicinal food and spices with potential antioxidative properties. METHODS:In vitro glucose-bovine serum albumin (BSA) assay was used. Ethanolic extracts of ten common household condiments/herbs ...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(13)60097-8
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR. METHODS:In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60087-0
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:To investigate antiviral activity of maca to reduce viral load in Madin-Darby canine kidney (MDCK) cells infected with influenza type A and B viruses (Flu-A and Flu-B, respectively). METHODS:Maca were extracted with methanol (1:2, v/v). The cell viability and toxicity of the extracts were evaluated on MDCK c...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/S1995-7645(14)60268-6
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To evaluate a new pharmacological activity/effect of linolenic acid (α- and γ-form) and conjugated-linoleic acid (CLA) causing antibacterial activity against Mycobacterium tuberculosis (Mtb). METHODS:The anti-Mtb activity/effect of linolenic acid and CLA were determined using different anti-Mtb indicator met...
journal_title：Asian Pacific journal of tropical medicine
pub_type： 杂志文章
doi：10.1016/j.apjtm.2016.01.021
更新日期：2016-02-01 00:00:00
