Abstract:
:The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting L4 control. The phenotypes of these mutants demonstrate that L4 regulates both transcription and translation of the S10 operon. Several mutations abolish both levels of L4 control; others eliminate either transcriptional or translational control with little or no effect on the other mode of regulation. We conclude that L4-mediated transcriptional and translational control share some sequence requirements, but the two regulatory processes recognize somewhat different features of the S10 leader. Primary as well as secondary structures within the S10 leader appear to be involved.
journal_name
Proc Natl Acad Sci U S Aauthors
Freedman LP,Zengel JM,Archer RH,Lindahl Ldoi
10.1073/pnas.84.18.6516subject
Has Abstractpub_date
1987-09-01 00:00:00pages
6516-20issue
18eissn
0027-8424issn
1091-6490journal_volume
84pub_type
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更新日期:2008-08-26 00:00:00