Abstract:
BACKGROUND:The genome of Pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism of interest in lignocellulose biorefineries due to its ability to delignify agricultural wastes, was sequenced with the purpose of identifying and characterizing the enzymes responsible for lignin degradation. RESULTS:Heterologous expression of the class II peroxidase genes, followed by kinetic studies, enabled their functional classification. The resulting inventory revealed the absence of lignin peroxidases (LiPs) and the presence of three versatile peroxidases (VPs) and six manganese peroxidases (MnPs), the crystal structures of two of them (VP1 and MnP4) were solved at 1.0 to 1.1 Å showing significant structural differences. Gene expansion supports the importance of both peroxidase types in the white-rot lifestyle of this fungus. Using a lignin model dimer and synthetic lignin, we showed that VP is able to degrade lignin. Moreover, the dual Mn-mediated and Mn-independent activity of P. ostreatus MnPs justifies their inclusion in a new peroxidase subfamily. The availability of the whole POD repertoire enabled investigation, at a biochemical level, of the existence of duplicated genes. Differences between isoenzymes are not limited to their kinetic constants. Surprising differences in their activity T50 and residual activity at both acidic and alkaline pH were observed. Directed mutagenesis and spectroscopic/structural information were combined to explain the catalytic and stability properties of the most interesting isoenzymes, and their evolutionary history was analyzed in the context of over 200 basidiomycete peroxidase sequences. CONCLUSIONS:The analysis of the P. ostreatus genome shows a lignin-degrading system where the role generally played by LiP has been assumed by VP. Moreover, it enabled the first characterization of the complete set of peroxidase isoenzymes in a basidiomycete, revealing strong differences in stability properties and providing enzymes of biotechnological interest.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
Fernández-Fueyo E,Ruiz-Dueñas FJ,Martínez MJ,Romero A,Hammel KE,Medrano FJ,Martínez ATdoi
10.1186/1754-6834-7-2subject
Has Abstractpub_date
2014-01-03 00:00:00pages
2issue
1issn
1754-6834pii
1754-6834-7-2journal_volume
7pub_type
杂志文章abstract:Background:Inefficient utilization of glycerol by Clostridium beijerinckii (Cb) is a major impediment to adopting glycerol metabolism as a strategy for increasing NAD(P)H regeneration, which would in turn, alleviate the toxicity of lignocellulose-derived microbial inhibitory compounds (LDMICs, e.g., furfural), and impr...
journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1388-9
更新日期:2019-03-09 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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doi:10.1186/s13068-017-0859-0
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-016-0460-y
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-019-1378-y
更新日期:2019-02-27 00:00:00
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journal_title:Biotechnology for biofuels
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-020-01788-8
更新日期:2020-08-26 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-6
更新日期:2012-02-13 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-6-134
更新日期:2013-09-16 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2012-09-07 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2020-03-11 00:00:00
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更新日期:2018-04-02 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/s13068-021-01878-1
更新日期:2021-01-19 00:00:00
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更新日期:2017-03-29 00:00:00
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pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
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更新日期:2020-05-18 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 社论
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更新日期:2009-09-01 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2018-07-18 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2014-08-27 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2018-12-18 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
doi:10.1186/1754-6834-5-30
更新日期:2012-05-06 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2019-09-30 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2011-02-10 00:00:00
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journal_title:Biotechnology for biofuels
pub_type: 杂志文章
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更新日期:2014-12-31 00:00:00