Abstract:
:Ia molecule (Ek,b beta:Ek alpha or Ak beta:Ak alpha)-containing planar membranes were constructed with cholesterol and a 9:1 molar ratio of the phospholipids dipalmitoyl phosphatidylcholine and dilinoleoyl phosphatidylcholine. This lipid composition was found to be optimal for the stimulation of T cell hybridomas of different specificities. Use of this system allowed the detection of weak responses not measurable when other artificial membranes were used. Activation of the cytochrome c, Ek,b beta:Ek alpha-reactive hybridoma 2B4.11 using such membranes resulted in responses comparable to those found using antigen-presenting cells (APC); that is, similar amounts of IL-2 were produced at the same concentrations of antigenic peptides. Presentation of moth and pigeon cytochrome c peptides by Ek beta:Ek alpha- or Eb beta:Ek alpha-reconstituted membranes resulted in 2B4.11 response patterns similar to those previously described using B10.A or B10.A(5R) APC. These data conclusively demonstrate that differential stimulation by moth and pigeon cytochrome c peptides depends solely on structural differences in the E beta:E alpha molecules used for antigen presentation.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Quill H,Carlson L,Fox BS,Weinstein JN,Schwartz RHdoi
10.1016/0022-1759(87)90432-7subject
Has Abstractpub_date
1987-04-02 00:00:00pages
29-41issue
1eissn
0022-1759issn
1872-7905pii
0022-1759(87)90432-7journal_volume
98pub_type
杂志文章abstract::A lectin isolated from the tropical jackfruit, jacalin, previously reported to precipitate human immunoglobulin A (IgA), and conjugated to agarose was used to separate the two subclasses of IgA from secretions. Jacalin-agarose binds specifically to the D-galactose moiety of IgA1 but not to IgA2 which has a different c...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(87)90037-8
更新日期:1987-05-04 00:00:00
abstract::The method of extraction of outer membrane proteins (OMPs), the conditions of electrophoretic transfer, and the conditions of antibody binding, were compared in Western blotting studies of Neisseria meningitidis outer membrane antigens. The OMP profiles obtained by SDS-PAGE of outer membrane vesicles extracted with li...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90383-7
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abstract::We describe an intracellular cytokine staining, flow cytometry based technique for quantitative and qualitative assessment of CD8+ T lymphocyte-mediated antigen specific immune responses. This adaptation of the technique first described by Suni et al. [J. Immunol. Methods, 212 (1998) 89; Cytometry, 40 (2000) 60] utili...
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更新日期:2002-04-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90330-2
更新日期:1979-01-01 00:00:00
abstract::A new adhesion assay was developed that utilizes buoyancy, rather than washing or centrifugation, to remove non-adherent cells. Biotinylated cells were added to wells containing cell monolayers or purified protein substrates. Non-adherent cells were then removed by floatation on a dense Percoll solution. The adherent ...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00187-6
更新日期:1995-12-01 00:00:00
abstract::Many research and clinical applications require large quantities of full-length antibodies with long circulating half-lives, and production of these complex multi-subunit proteins has in the past been restricted to eukaryotic hosts. In this report, we demonstrate that efficient secretion of heavy and light chains in a...
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pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
abstract::A microscale ELISA system, designated as the 'C1q-micro ELISA,' has been developed for the measurement of circulating immune complexes. The sensitivity of the method ranged between 0.1 and 3 microgram equivalents of aggregated human gamma-globulins. The procedure requires small quantities of serum and reagents, and it...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(82)90308-8
更新日期:1982-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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更新日期:2000-09-21 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(91)90278-n
更新日期:1991-09-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:Interleukin-13 (IL-13) has been difficult to quantify within human serum due to low abundance. Available assays have not been sensitive enough to detect IL-13 at the femtogram level. Thus, there are inconsistencies within the published literature as to IL-13 concentrations in normal or disease populations. To...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2015.08.006
更新日期:2015-11-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 信件
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更新日期:1997-03-28 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2005-10-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(86)90543-0
更新日期:1986-03-13 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(88)90315-8
更新日期:1988-11-25 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90160-0
更新日期:1994-12-28 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1988-11-10 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
doi:10.1016/j.jim.2015.03.017
更新日期:2015-07-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(90)90387-b
更新日期:1990-12-05 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90288-5
更新日期:1992-11-05 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(86)90430-8
更新日期:1986-10-23 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(82)90226-5
更新日期:1982-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2010.02.003
更新日期:2010-04-15 00:00:00
abstract::A sensitive procedure based on the use of iodinated antibody allows the detection of antigenic material by double-diffusion analysis. An autoradiographed immunodiffusion pattern reveals distinct radio-labeled precipitation lines for an extract of yeast with wild type HIS4 protein and for extracts of mutants containin...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(78)90048-0
更新日期:1978-01-01 00:00:00