Abstract：
:Many research and clinical applications require large quantities of full-length antibodies with long circulating half-lives, and production of these complex multi-subunit proteins has in the past been restricted to eukaryotic hosts. In this report, we demonstrate that efficient secretion of heavy and light chains in a favorable ratio leads to the high-level expression and assembly of full-length IgGs in the Escherichia coli periplasm. The technology described offers a rapid, generally applicable and potentially inexpensive method for the production of full-length therapeutic antibodies, as verified by the expression of several humanized IgGs. One E. coli-derived antibody in particular, anti-tissue factor IgG1, has been thoroughly evaluated and has all of the expected properties of an aglycosylated antibody, including tight binding to antigen and the neonatal receptor. As predicted, the protein lacks binding to C1q and the FcgammaRI receptor, making it an ideal candidate for research purposes and therapeutic indications where effector functions are either not required or are actually detrimental. In addition, a limited chimpanzee study suggests that the E. coli-derived IgG1 retains the long circulating half-life of mammalian cell-derived antibodies.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Simmons LC,Reilly D,Klimowski L,Raju TS,Meng G,Sims P,Hong K,Shields RL,Damico LA,Rancatore P,Yansura DGdoi
10.1016/s0022-1759(02)00036-4keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
133-47issue
1-2eissn
0022-1759issn
1872-7905pii
S0022175902000364journal_volume
263pub_type
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