Abstract:
:A simple technique for raising specific antiserum to the native molecule of Epstein-Barr virus (EBV)-encoded nuclear antigen-2 (EBNA-2) from Raji cells is described. The procedure involves the use of immunoblotting to identify the EBNA-2 polypeptide followed by subsequent excision from an SDS-gel and immunization of experimental animals. The anti-EBNA-2 antiserum recognized a single polypeptide of 86-87 kDa on immunoblots prepared from extracts of EBV-positive Raji or B95-8 cells, while it did not react with any proteins form P3HR-1 or Daudi cells, which carry EBNA-2-defective virus. The method might be applicable to other systems where isolation of purified proteins for immunization is either difficult or unfeasible.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Dewhurst S,Volsky DJdoi
10.1016/0022-1759(86)90543-0subject
Has Abstractpub_date
1986-03-13 00:00:00pages
283-7issue
2eissn
0022-1759issn
1872-7905pii
0022-1759(86)90543-0journal_volume
87pub_type
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更新日期:2000-02-03 00:00:00
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