A new rapid rosette-forming cell micromethod for the detection of antibody-synthesizing hybridomas.

abstract：:Chinese hamster lymphocyte cultures were supplemented with foetal calf serum (FCS) or hose serum (HS). Addition of 2-mercaptoethanol (2-ME) to cultures supplemented with these sera resulted in a marked increase lymphocyte proliferation. The PHA concentrations necessary for optimal lymphocyte stimulation were much lowe...

journal_title：Journal of immunological methods

authors： De Jong B,Anders GJ,Van der Meer IH,Zijlstra J,Idenburg VJ

更新日期：1982-01-01 00:00:00

abstract：:An inhibitor to von Willebrand factor (vWf) was detected in the plasma from two patients with histories of mild bleeding and one patient with a severe deficiency in the Factor VIII complex using a competitive enzyme-linked immunosorbent assay (ELISA) procedure. IgG antibodies from the patients' plasmas were shown to b...

journal_title：Journal of immunological methods

authors： Stewart MW,Etches WS,Shaw AR,Gordon PA

更新日期：1997-01-15 00:00:00

abstract：:A method is described for the isolation of filamentous phage-displayed human monoclonal antibodies directed at unpurifiable cell surface-expressed molecules. To optimize the capture of antigen-specific phage and minimize the binding of irrelevant phage antibodies, a simultaneous positive and negative selection strateg...

journal_title：Journal of immunological methods

authors： Siegel DL,Chang TY,Russell SL,Bunya VY

更新日期：1997-08-07 00:00:00

abstract：:Functional analysis of antigen-specific CD8(+) T cells is important for understanding the immune response in various immunological disorders. To analyze CD8(+) T cell responses to a variety of antigens with no readily defined peptides available, we developed a system using CD4(+) phytohemagglutinin (PHA) blasts transd...

journal_title：Journal of immunological methods

authors： Naota H,Miyahara Y,Okumura S,Kuzushima K,Akatsuka Y,Hiasa A,Kitano S,Takahashi T,Yuta A,Majima Y,Shiku H

更新日期：2006-07-31 00:00:00

abstract：:An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

journal_title：Journal of immunological methods

authors： Madsen RD,Saeed FA,Coates SR

更新日期：1987-11-05 00:00:00

abstract：:A simple semi-automated method for the assay of large numbers of replicate microcultures for the presence of antibody-forming cell clones is described. The supernatant medium is removed from microcultures by a single sharp flick on inverting the tray. The cultured cells are mixed with 0.05 ml of a plaque-revealing mix...

journal_title：Journal of immunological methods

authors： Pike BL,Jennings G,Shortman K

更新日期：1982-01-01 00:00:00

abstract：:A 24 h, highly sensitive assay for detection of interleukin 1 (IL-1) is described. A thioguanine-resistant mutant of the murine lymphoma cell line LBRM 33 was selected (LBRM TG6). When this cell line was incubated with low concentrations of PHA and IL-1 it produced interleukin 2 (IL-2). IL-2-dependent HT2 cells were c...

journal_title：Journal of immunological methods

authors： Larrick JW,Brindley L,Doyle MV

更新日期：1985-05-10 00:00:00

abstract：:Overlapping peptide scans prepared by Spot synthesis have been used to map interaction sites in several systems. Here we report our experience with this approach to identify peptides from the variable parts of anti-hapten, anti-peptide and anti-protein antibodies that retain their specific antigen-binding capacity in ...

journal_title：Journal of immunological methods

authors： Laune D,Molina F,Ferrières G,Villard S,Bès C,Rieunier F,Chardès T,Granier C

更新日期：2002-09-01 00:00:00

abstract：:Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach tha...

journal_title：Journal of immunological methods

authors： Dzutsev AK,Belyakov IM,Isakov DV,Gagnon SJ,Margulies DH,Berzofsky JA

更新日期：2008-04-20 00:00:00

abstract：:A new method is described for demonstrating the individual subclasses of human IgG immunoglobulins by means of analytical isotachophoresis. Individual subclasses of IgG purified from sera of multiple myeloma patients have distinct mobility patterns. When a mixture with purified IgG for each of the 4 subclasses is anal...

journal_title：Journal of immunological methods

authors： Hedlund KW,Wistar R Jr,Nichelson D

更新日期：1979-01-01 00:00:00

abstract：:A mild procedure for the purification of rabbit Fab' antibodies is described. A small column of normal goat IgG-Sepharose 4B (3 cm x 5mm) was saturated with F(ab')2 prepared from rabbit anti-goat IgG, and anti-goat IgG Fab was eluted from the column by splitting the disulfide bond in the hinge of the F(ab')2 molecule ...

journal_title：Journal of immunological methods

authors： Ishikawa E,Yoshitake S

更新日期：1980-01-01 00:00:00

abstract：:Natural killer (NK) cells are important mediators of virus- and tumor-specific immune responses. The transfection of genes into NK cells has been proven difficult and so far requires infection with virus-based vectors. Here, the application of a novel nonviral, electroporation-based gene transfer method is described f...

journal_title：Journal of immunological methods

authors： Trompeter HI,Weinhold S,Thiel C,Wernet P,Uhrberg M

更新日期：2003-03-01 00:00:00

abstract：:In vitro differentiation of human monocytes (Mo) provides large amounts of mature and functionally competent macrophages (M phi) which may be used as potentially powerful anticancer agents for adoptive immunotherapy. Granulocyte macrophage-colony stimulating factor (GM-CSF) was evaluated for its ability to influence l...

journal_title：Journal of immunological methods

authors： Eischen A,Vincent F,Bergerat JP,Louis B,Faradji A,Bohbot A,Oberling F

更新日期：1991-10-25 00:00:00

abstract：:We developed a noncompetitive two-site sandwich ELISA to quantitate monoclonal antibodies in culture supernatant. This assay measures the initial enzyme activity rate during the first minute of the reaction, which ensures linear velocity relative to time and a progress curve slope proportional to analyte concentration...

journal_title：Journal of immunological methods

authors： Domínguez M,Moreno I,Toraño A

更新日期：2019-11-01 00:00:00

abstract：:The sensitivity of any immunoassay is a complex function of the underlying physico-chemical basis of the technique and the size and source of 'experimental' errors. Analysis of this relationship emphasizes the distinction between competitive labelled analyte methods, e.g., RIA, and the non-competitive labelled antibod...

journal_title：Journal of immunological methods

authors： Jackson TM,Ekins RP

更新日期：1986-02-27 00:00:00

abstract：:Typical clinical grade human IgG (intravenous immunoglobulin, IVIG), used for carbohydrate analysis, is derived from thousands of healthy donors. Quantitative high-resolution glycan profiles of IgG and its Fc-Fab fragments are presented here. Glycan profiles were established following digestions with Fc specific endog...

journal_title：Journal of immunological methods

authors： Anumula KR

更新日期：2012-08-31 00:00:00

abstract：:An ELISA method for the detection of monoclonal antibodies (MAb) to the T3-T cell antigen receptor (TCR) complex was devised. The T3-TCR complex was solubilised using digitonin and a rat anti-T3 MAb (Campath 3) was used to bind it to an ELISA plate. Normal rat serum was used to block cross-reactivity between the rat M...

journal_title：Journal of immunological methods

authors： Clark DM,Boylston AW

更新日期：1988-01-21 00:00:00

abstract：:A high-capacity manifold support with immobilized antigen was developed for the analysis of IgE-mediated immune reactivity in allergic subjects. Using this 96-pronged support, specific antibodies were trapped and detected from large sets of serum samples. We describe the binding of large amounts of antigen onto the ex...

journal_title：Journal of immunological methods

authors： Kwiatkowski M,Parik J,Landegren U

更新日期：1994-01-12 00:00:00

abstract：:Observations that the innate arm of the immune system is upregulated in pregnancy have highlighted the need for methods of isolating pure populations of monocytes for studies into pregnancy and pre-eclampsia without activating them during the isolation process. Density gradient centrifugation using iodixanol is a usef...

journal_title：Journal of immunological methods

authors： Nutt JC,Willis CC,Morris JM,Gallery ED

更新日期：2004-10-01 00:00:00

abstract：:In order to isolate monoclonal antibodies (mAbs) that bind to tumor-associated antigens (Ags) we developed the following strategy. Using the phage-display Ab library we isolated a large number of mAbs that bind to the surface of human tumor cells. The mAbs were individually screened by immunostaining, and clones that ...

journal_title：Journal of immunological methods

authors： Kurosawa G,Sumitomo M,Akahori Y,Matsuda K,Muramatsu C,Takasaki A,Iba Y,Eguchi K,Tanaka M,Suzuki K,Morita M,Sato N,Sugiura M,Sugioka A,Hayashi N,Kurosawa Y

更新日期：2009-12-31 00:00:00

abstract：:A technically simple microassay for macrophage migration inhibition studies is described. Using simple 0.08 ml of test solution per assay, meaningful statistical analysis via multiple replicates is possible. A novel feature is that test solutions may be stored frozen in the test well until the indicating macrophages a...

journal_title：Journal of immunological methods

authors： Coyle DE,Brammer PW,Halsall HB

更新日期：1980-01-01 00:00:00

abstract：:Cell-enzyme-linked immunosorbent assay (cell-ELISA) is a technique for the rapid, convenient, and quantitative detection of molecules expressed on the cell surface. Here we present an evaluation of beta-galactosidase as an antibody-tag for cell-ELISA. In contrast to substrates for horseradish peroxidase (HRP) and alka...

journal_title：Journal of immunological methods

authors： Liu Z,Gurlo T,von Grafenstein H

更新日期：2000-02-03 00:00:00

abstract：:A C1q solid phase microassay was designed for the rapid detection of circulating immune complexes. Its level of sensitivity is comparable to that of the Raji cell and greater than the C1q binding assay; furthermore, it is faster and low in cost. These conditions make it more practical and applicable in the clinical se...

journal_title：Journal of immunological methods

authors： Orozco A,Contreras CE,Sánchez P,Meilijson O,Bianco NE

更新日期：1983-04-29 00:00:00

abstract：:A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish them from erythrocy...

journal_title：Journal of immunological methods

authors： Goldberg NH,Kenady DE,Super BS,Chretien PB

更新日期：1976-01-01 00:00:00

abstract：:A study was undertaken to establish conditions of growth to increase C3b receptor synthesis on a human B lymphoblastoid cell line (Raji) by use of cell differentiation effectors. It appears that whereas two polar compounds HMBA (2mM) and Me2SO(2%) have no or little effect, 5 BrdU (30microM) and db cAMP (5 x10(-4) M) a...

journal_title：Journal of immunological methods

authors： Barel M,Charriaut C,Frade R

更新日期：1981-01-01 00:00:00

abstract：:We report a novel competitive type immunoassay for oestradiol based on the idiotypic anti-idiotypic approach. This has been achieved by the production of an anti-idiotypic antibody (anti-Id) which is directed against the oestradiol binding site of the primary idiotypic antibody (Ab1). In this format the primary Ab1 wa...

journal_title：Journal of immunological methods

authors： Altamirano-Bustamante A,Barnard G,Kohen F

更新日期：1991-04-08 00:00:00

abstract：:A microculture and radioimmunoassay is described which is capable of generating and detecting very small levels of human anti-DNP antibody. The antibody produced is hapten specific and the response is T-cell dependent. The microculture system described avoids the need for high serum supplements, mitogens, in vivo immu...

journal_title：Journal of immunological methods

authors： Macdonald PR,Simpson EJ,Crosier PS

更新日期：1983-12-16 00:00:00

abstract：:The metaphase index assay (MIA) for thyroid growth stimulators, as originally described, used FRTL-5 thyroid cells cultured in Bellco culture chambers and glass microscope slides. The metaphases were observed using the nuclear strain aceto-lacto orcein. However the surface properties of the glass proved to be variable...

journal_title：Journal of immunological methods

authors： Ealey PA,Mitchell SD,Rowles PM,Marshall NJ

更新日期：1988-06-28 00:00:00

abstract：:Electrochemiluminescence (ECL) assays have been widely used for the detection of anti-therapeutic antibodies (ATAs) against biotherapeutics. With the discontinuation of BioVeris (BV) ECL platform, an alternative technology was needed to replace BV assays to ensure continuous support of multi-year clinical studies. Aft...

journal_title：Journal of immunological methods

authors： Qiu ZJ,Ying Y,Fox M,Peng K,Lewin-Koh SC,Coleman D,Good J,Lowe J,Rahman A,Yang J,Jiang J,Quarmby V,Song A

更新日期：2010-10-31 00:00:00

abstract：:A method for coating native, non-derivatized, polysaccharide (PS) onto nitrocellulose (NC) for identifying PS-specific antibodies has been developed. The new feature of this method is that PS molecules are vacuum filtered onto NC in their native state by devices that can accommodate NC of different sizes and shapes. P...

journal_title：Journal of immunological methods

authors： Feng SH,Rubinstein LJ,Stein KE

更新日期：1991-03-21 00:00:00