An improved assay for the detection of interleukin 1.

abstract：:We have developed a double monoclonal sandwich enzyme immunoassay to measure smooth muscle myosin heavy chain (MHC). Analytical performance of the assay showed reliable detection of smooth muscle MHC in human sera. The mean of the smooth muscle MHC level in normal human sera was 0.9 +/- 0.9 ng/ml. In sera of patients ...

journal_title：Journal of immunological methods

authors： Katoh H,Suzuki T,Yokomori K,Suzuki S,Ohtaki E,Watanabe M,Yazaki Y,Nagai R

更新日期：1995-09-11 00:00:00

abstract：:A simple method to study 19S anti-SRBC antibody production is described. This technique, performed by incubation of immune spleen cells, SRBC and C in the fluid phase, utilises photomeric estimation of antibody mediated haemoglobin release. The test is quicker and more accurate than the two-step haemolytic plaque assa...

journal_title：Journal of immunological methods

authors： Van Dijk H,Bloksma N

更新日期：1977-01-01 00:00:00

abstract：:Quantitative indirect immunofluorescence (QIIF) methods used to measure absolute numbers of surface-expressed antigens have produced conflicting results [Marchant, A., Duchow, J., Delville, J., Goldman, M., 1992. Lipopolysaccharide induces up-regulation of CD14 molecule on monocytes in human whole blood. European Jour...

journal_title：Journal of immunological methods

authors： Smith KB,Ellis SA

更新日期：1999-08-31 00:00:00

abstract：:We describe the preparation of a new rat monoclonal antibody (CRC1) to the N-terminal sequence of the 43 kDa subunit of human ovarian inhibin, and its use together with other anti-peptide monoclonal antibodies, in two-site immunoassays for the detection of inhibin-related material in biological fluids. The Fab fractio...

journal_title：Journal of immunological methods

authors： Groome N,O'Brien M

更新日期：1993-10-15 00:00:00

abstract：:Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers an...

journal_title：Journal of immunological methods

authors： Becker C,Hoschützky H,Rist W,Lenter M,Schneider FJ,Jonuleit H

更新日期：2010-02-28 00:00:00

abstract：:A new rapid staining and measuring method has been developed for the quantification of migrated cells in a microchemotaxis chamber. The migrated cells were, after staining, evaluated by a transmission densitometer. The method introduced here is more accurate and faster than those described previously. In addition the ...

journal_title：Journal of immunological methods

authors： Klomp JP,Te Velde AA,Figdor CG

更新日期：1989-03-10 00:00:00

abstract：:Interferons-alpha and -beta (IFN-alpha and -beta) are cytokines that are widely known to induce potent anti-viral activity. However, it has become increasingly apparent that IFN-alpha and -beta exert a variety of other biological effects, including anti-tumour and immunomodulatory activities and are increasingly used ...

journal_title：Journal of immunological methods

authors： Mire-Sluis AR,Page LA,Meager A,Igaki J,Lee J,Lyons S,Thorpe R

更新日期：1996-09-09 00:00:00

abstract：:In the context of allogeneic bone marrow transplantation, an accurate estimate of the risk of developing graft-versus-host disease (GVHD) is of major interest. The pre-transplant frequency of donor's helper T-lymphocyte precursors (HTLp) directed against host's antigens may be helpful in predicting this risk. This tec...

journal_title：Journal of immunological methods

authors： Winandy M,Lewalle P,Deneys V,Ferrant A,De Bruyère M

更新日期：1998-06-01 00:00:00

abstract：:A competitive enzyme immunoassay with labelled antibodies has been developed for methotrexate (MTX). Methotrexate in the sample and a constant quantity of this hapten physically absorbed to polystyrene spheres through a methylated bovine albumin carrier were allowed to compete for a limiting amount of peroxidase label...

journal_title：Journal of immunological methods

authors： Ferrua B,Milano G,Ly B,Guennec JY,Masseyeff R

更新日期：1983-05-27 00:00:00

abstract：:Analytical affinity electrophoresis experiments are described as analogues of column and batch affinity experiments. The are based on crossed immunoelectrophoresis with included affinity, here concanavalian A (con A) and con A-Sepharose. Reactions with serum proteins and allergens from cow's hair and dander are descri...

journal_title：Journal of immunological methods

authors： Bøg-Hansen TC,Prahl P,Løwenstein H

更新日期：1978-01-01 00:00:00

abstract：:An assay has been developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis is quantitated spectrophotometrically (QHS). Immune mouse spleen cells, SRBC and guinea pig complement were incubated at 37 degrees C for 1 h in buffer, centrifuged, and the supernatant solutions read...

journal_title：Journal of immunological methods

authors： Simpson MA,Gozzo JJ

更新日期：1978-01-01 00:00:00

abstract：:2-Methoxyestradiol (2ME), an end-metabolite of 17beta-estradiol, is an antiproliferative agent that is currently being tested in clinical trials for cancer treatment. We hereby report that sub-cytotoxic concentrations of 2ME influence the in vitro proliferation of human peripheral blood B lymphocytes. More surprisingl...

journal_title：Journal of immunological methods

authors： Cayer MP,Drouin M,Proulx M,Jung D

更新日期：2010-04-15 00:00:00

abstract：:IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is ...

journal_title：Journal of immunological methods

authors： Janot-Sardet C,Assouline B,Cheynier R,Morre M,Beq S

更新日期：2010-02-28 00:00:00

abstract：:This work presents a method for analyzing protein microarrays using a colorimetric nanogold probe coupled with silver enhancement (gold-silver detection). In this method, the gold nanoparticles were introduced to the microarray by the specific binding of the gold-conjugated antibodies or streptavidins and then coupled...

journal_title：Journal of immunological methods

authors： Liang RQ,Tan CY,Ruan KC

更新日期：2004-02-15 00:00:00

abstract：:A purification method for a human urinary glycoprotein (HGP92) dissociated from Tamm Horsfall protein (THP) is described. Tamm-Horsfall protein, obtained by salt precipitations, was again precipitated in presence of monovalent ions. In these conditions, Tamm-Horsfall protein displayed a tendency to form a gel. After u...

journal_title：Journal of immunological methods

authors： Fontan E,Jusforgues-Saklani H,Briend E,Fauve RM

更新日期：1995-11-16 00:00:00

abstract：:Wegener's granulomatosis (WG) is a systemic vasculitis which is diagnosed on clinicopathological findings. The diagnosis may be aided by the presence of anti-neutrophil cytoplasm antibodies (ANCA). In WG, ANCA are primarily directed to proteinase 3 (PR3), a serine protease of the azurophilic granules of the neutrophil...

journal_title：Journal of immunological methods

authors： Baslund B,Petersen J,Permin H,Wiik A,Wieslander J

更新日期：1994-10-14 00:00:00

abstract：:Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC...

journal_title：Journal of immunological methods

authors： Joshi L,Ponnana M,Sivangala R,Chelluri LK,Nallari P,Valluri VL,Gaddam S

更新日期：2016-05-01 00:00:00

abstract：:An immunoblot (Western) assay was developed employing a species-specific monoclonal antibody to a 43 kDa Mycoplasma pneumoniae membrane polypeptide and a species-specific monoclonal antibody to 29 kDa Legionella pneumophila outer membrane protein. This assay could simultaneously detect these two different antigens dir...

journal_title：Journal of immunological methods

authors： Madsen RD,Saeed FA,Coates SR

更新日期：1987-11-05 00:00:00

abstract：:We describe the setting up and validation of a reporter gene assay for type I IFN based on monkey Vero cells transfected with pMx-Luc, a plasmid carrying the luciferase gene under the control of the type I IFN inducible mouse Mx1 promoter. Vero cells were stably transfected with pMx-Luc and clone 3-143/5 was selected ...

journal_title：Journal of immunological methods

authors： Canosi U,Mascia M,Gazza L,Serlupi-Crescenzi O,Donini S,Antonetti F,Galli G

更新日期：1996-11-29 00:00:00

abstract：:Pig peripheral blood lymphocytes, typed for LD antigens by negative reactions to homozygous cells in primary MLRs and for SD antigens by complement-mediated lymphocytotoxicity, were sensitised in vitro against X-irradiated lymphocytes. These "primed" cells were rechallenged with lymphocytes possessing a range of LD an...

journal_title：Journal of immunological methods

authors： Pawelec GP

更新日期：1978-01-01 00:00:00

abstract：:Human properdin was prepared from the zymosan eluate or euglobulin fraction of serum by immunoadsorbent column chromatography. An immunoadsorbent column containing antibody to human serum was employed to remove serum proteins other than properdin from zymosan eluates. Immunoadsorbent with antibody to properdin was use...

journal_title：Journal of immunological methods

authors： Konno T,Hirai H

更新日期：1976-01-01 00:00:00

abstract：:Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based o...

journal_title：Journal of immunological methods

authors： Houthuys E,Movahedi K,De Baetselier P,Van Ginderachter JA,Brouckaert P

更新日期：2010-07-31 00:00:00

abstract：:A proliferation assay to detect a specific T cell response against HLA class I-binding peptides is reported. To establish the specificity and sensitivity of the assay we used a synthetic peptide derived from the CMV glycoprotein B containing the HLA-A2.1 ligand motif. Lymphocyte proliferation was measured following cu...

journal_title：Journal of immunological methods

authors： Mayer S,Scheibenbogen C,Lee KH,Keilholz W,Stevanovic S,Rammensee HG,Keilholz U

更新日期：1996-10-16 00:00:00

abstract：:Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not cause cell activat...

journal_title：Journal of immunological methods

authors： Abewe H,Deshmukh S,Mukim A,Beliakova-Bethell N

更新日期：2020-01-01 00:00:00

abstract：:A recombinant retroviral vector with an inserted bacterial neomycin resistance (neo) gene was used to transfer in vitro neomycin resistance (neoR) into murine cytotoxic lymphocyte precursors (CLP). The infection protocol involved co-cultivation of mitogen-activated splenic T-blasts with irradiated cells that produced ...

journal_title：Journal of immunological methods

authors： Reimann J,Heeg K,Wagner H,Keller G,Wagner EF

更新日期：1986-05-01 00:00:00

abstract：:A simple technique for raising specific antiserum to the native molecule of Epstein-Barr virus (EBV)-encoded nuclear antigen-2 (EBNA-2) from Raji cells is described. The procedure involves the use of immunoblotting to identify the EBNA-2 polypeptide followed by subsequent excision from an SDS-gel and immunization of e...

journal_title：Journal of immunological methods

authors： Dewhurst S,Volsky DJ

更新日期：1986-03-13 00:00:00

abstract：:A dot-ELISA technique for the detection of G. lamblia specific antigen in stool eluates of clinical cases of giardiasis was developed and evaluated employing monospecific antibodies to a G. lamblia specific coproantigen with a molecular mass of 66 kDa. The assay detected 22 (91.7%) of the 24 microscopically confirmed ...

journal_title：Journal of immunological methods

authors： Vinayak VK,Dutt P,Puri M

更新日期：1991-03-21 00:00:00

abstract：:The discovery of a naturally occurring missense point mutation in the gene encoding Fas-ligand (FasL/CD95L) in generalized lymphoproliferative disease (gld) mice has lead to the characterization of FasL as an important mediator of apoptosis. Further analysis of FasL function can be facilitated by crossing the gld muta...

journal_title：Journal of immunological methods

authors： Hoek RM,Kortekaas MC,Sedgwick JD

更新日期：1997-12-15 00:00:00

abstract：:This study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax antigen. TNF-alpha and IFN-gamma production from memory CD4...

journal_title：Journal of immunological methods

authors： Shinn AH,Bravo NC,Maecker HT,Smith JW

更新日期：2003-11-01 00:00:00

abstract：:A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring ser...

journal_title：Journal of immunological methods

authors： Weller FE,Mutchnick MG,Keren DF,Goldstein AL,Naylor PH

更新日期：1985-06-12 00:00:00