Abstract:
:Analysis of continuous culture methodology suggests that this potentially powerful tool for kinetic analysis can be improved by minimizing several inherent shortcomings. Medium background substrates - organic carbon, phosphate, and manganese - were shown to dominate kinetic observations at concentrations below chemical detection methods. Reactor wall growth, culture size distribution changes, sample removal-induced steady state perturbations, and limiting substrate leakage from organisms are treated in terms of kinetic measurement errors. Large variations in maximal growth rates and substrate uptake rates found are attributed to experimental protocol-induced transient states. Relationships are presented for correcting limiting substrate concentrations for lability during sampling, contamination with unreacted medium, and background substrate effects. Analytical procedures are discussed for improved measurement of limiting substrate kinetics involving enzymes, isotopes, and material balance manipulation. Relaxation methods as applied to continuous culture are introduced as a means for isolating separate rate constants describing net substrate transport and for evaluating cellular metabolite leakage. Low velocity growth, multiple substrate metabolism, and endogenous metabolism are discussed along with measurements showing that 1-month generation times for aquatic microorganisms can be quite normal and that the kinetics are compatible withμg/liter limiting substrate concentrations. The concept of regarding growth kinetics as the sum of several net accumulation processes is suggested.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Law AT,Robertson BR,Dunker SS,Button DKdoi
10.1007/BF02011647subject
Has Abstractpub_date
1975-12-01 00:00:00pages
261-83issue
4eissn
0095-3628issn
1432-184Xjournal_volume
2pub_type
杂志文章abstract::We investigated the impact of fish farm biodeposition on benthic bacteria, thraustochytrid protists, and heterotrophic protozoa (nanoflagellates and ciliates) in an oligotrophic area of the Mediterranean Sea. The fish farm impact was investigated both on a seagrass (Posidonia oceanica) bed and on soft bottom sediments...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0117-4
更新日期:2005-08-01 00:00:00
abstract::Bacteria of the family Rickettsiaceae (order Rickettsiales, alpha-Proteobacteria) are mainly known to be endosymbionts of arthropods with the capability to infect also vertebrate cells. Recently, they have also been found as leech endocytobionts. In the present paper, we report the first finding of a bacterium belongi...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0055-1
更新日期:2005-04-01 00:00:00
abstract::Muscodor spp. are proficient producers of bioactive volatile organic compounds (VOCs) with many potential applications. However, all members of this genus produce varying amounts and types of VOCs which suggests the involvement of epigenetics as a possible explanation. The members of this genus are poorly explored for...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0901-y
更新日期:2017-05-01 00:00:00
abstract::Metagenomics and the development of high throughput next generation sequencing capabilities have forced significant development in the field of phylogenetics: the study of the evolutionary relatedness of the planet's inhabitants. Herein, I review the major tree-building strategies, challenges and opportunities which e...
journal_title:Microbial ecology
pub_type: 杂志文章,评审
doi:10.1007/s00248-013-0236-x
更新日期:2013-07-01 00:00:00
abstract::Staphylococcus aureus, an opportunistic pathogen member of the nasal and skin microbiota, can also be found in human oral samples and has been linked to infectious diseases of the oral cavity. As the nasal and oral cavities are anatomically connected, it is currently unclear whether S. aureus can colonize the oral cav...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1291-0
更新日期:2019-08-01 00:00:00
abstract::> Abstract Claims that organisms can be cultured from amber, if substantiated, would be significant contributions to our understanding of the evolution, tenacity, and potential spread of life. Three reports on the isolation of organisms from amber have been published. Cano and Borucki recently reported the isolation o...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s002489900153
更新日期:1999-07-01 00:00:00
abstract::Interactions between bacteria and protozoa in soil were studied over 2-week periods in the field and in a pot experiment. Under natural conditions the total biological activity was temporarily synchronized by a large rainfall, and in the laboratory by the addition of water to dried-out soil, with or without plants. In...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02341429
更新日期:1981-12-01 00:00:00
abstract::Many sponges host abundant and active microbial communities that may play a role in the uptake of dissolved organic matter (DOM) by the sponge holobiont, although the mechanism of DOM uptake and metabolism is uncertain. Bulk and compound-specific isotopic analysis of whole sponge, isolated sponge cells, and isolated s...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-019-01410-x
更新日期:2020-02-01 00:00:00
abstract::The capacity to study the content and resolve the dynamics of the proteome of diverse microbial communities would help to revolutionize the way microbiologists study the function and activity of microorganisms in soil. To better understand the limitations of a proteomic approach to studying soil microbial communities,...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-009-9593-x
更新日期:2010-02-01 00:00:00
abstract::Scleractinian corals form the framework of coral reefs and host abundant and diverse microbial communities that are fundamental to their success. A very limited number of studies have examined the co-occurrence of multiple partners within the coral 'holobiont' and their pattern of specificity over different geographic...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1255-4
更新日期:2019-04-01 00:00:00
abstract::The high plasticity of root morphology, physiology, and function influences root-associated microbiomes. However, the variation in root-associated microbiome diversity and structures in response to root diameter at different root depths remains poorly understood. Here, we selected black locust (Robinia pseudoacacia L....
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01678-4
更新日期:2021-01-15 00:00:00
abstract::The aim of the study was to assess the effect of the mycorrhizosphere of A. tortillis spp. raddiana mycorrhized with Glomus intraradices on the root nodulation by Sinorhizobium terangae (ORS 1009) and/or Mesorhizobium plurifarium (ORS 1096) in two different culture substrates (sandy soil and sand). The endomycorrhizal...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-002-1022-3
更新日期:2003-02-01 00:00:00
abstract:CONCLUSION:As the interactions between marine invertebrates and their bacterial commensals and symbionts are better understood, the application of biotechnology will enhance both environmental and economic benefit. In the immediate future, marine bacteria, either selected or genetically engineered, will play a signific...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02153225
更新日期:1986-03-01 00:00:00
abstract::Methods have been explored for detection of methylotrophs in natural samples, using environmental primers based on genes involved in the tetrahydromethanopterin (H4MPT)-linked C1 transfer pathway. The underlying hypotheses were that the H4MPT-linked pathway is an ancient methylotrophy pathway, based on gene divergence...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0212-6
更新日期:2004-11-01 00:00:00
abstract::Septate endophytes proliferating in the roots of grasslands' plants shed doubts on the importance of arbuscular mycorrhizal (AM) symbioses in dry soils. The functionality and diversity of the AM symbioses formed in four replicates of three adjacent plant communities (agricultural, native, and restored) in Grasslands N...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-009-9629-2
更新日期:2010-05-01 00:00:00
abstract::To date, little is known about the diazotrophs in freshwater ecosystems. In this study, we examined the diversity, abundance, and distribution of the diazotrophic community in the deep oligotrophic Lake Fuxian using high-throughput sequencing and quantitative polymerase chain reaction of nifH genes. Our results showed...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-019-01461-0
更新日期:2020-05-01 00:00:00
abstract::Although organic-aggregate-associated bacteria play a pivotal role in microbial food webs and in the cycling of major elements, their community composition and diversity have not been extensively studied, especially in shallow freshwater systems. This study is among the first to explore intra-lake horizontal heterogen...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-008-9482-8
更新日期:2009-08-01 00:00:00
abstract::Carnivorous pitcher plants Sarracenia purpurea host diverse eukaryotic and bacterial communities which aid in insect prey digestion, but little is known about the functional processes mediated by the microbial communities. This study aimed to connect pitcher community diversity with functional nutrient transformation ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1187-z
更新日期:2018-11-01 00:00:00
abstract::It is suspected that phagotrophic marine protozoa might possess feeding receptors that enable them to discern the nutritional quality of individual prey items (during prey-handling) on the basis of their cell-surface biochemistry. This article reviews advances in our understanding of the molecular mechanisms that medi...
journal_title:Microbial ecology
pub_type: 杂志文章,评审
doi:10.1007/s00248-008-9421-8
更新日期:2009-05-01 00:00:00
abstract::The McMurdo Dry Valleys of Antarctica form the coldest and driest ecosystem on Earth. Within this region there are a number of perennially ice-covered (3-6 m thick) lakes that support active microbial assemblages and have a paucity of metazoans. These lakes receive limited allochthonous input of carbon and nutrients, ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-003-1007-x
更新日期:2004-05-01 00:00:00
abstract::Crop residues are a crucial ecological niche with a major biological impact on agricultural ecosystems. In this study, we used a combined diachronic and synchronic field experiment based on wheat-oilseed rape rotations to test the hypothesis that plant is a structuring factor of microbial communities in crop residues,...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-019-01340-8
更新日期:2019-05-01 00:00:00
abstract::By using substrate molecules of varying degrees of surface activity, we were able to measure some features of bacterial activity in the surface microlayers (SM) and in the subsurface (bulk) water. The fraction of active cells was determined by a combined microautoradiography-epifluorescence (ME) method. Measurements w...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02019021
更新日期:1983-12-01 00:00:00
abstract::Ecophysiological investigations on the salinity acclimation of the cyanobacterium Prochlorothrix hollandica SAG 10.89 led to significantly revised salinity tolerance limits. Besides potential effects of cultivation techniques, clear ion composition effects mainly explain formerly described hypersensitivity to NaCl-med...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-007-9311-5
更新日期:2008-05-01 00:00:00
abstract::Generally, only a small fraction of free-living pelagic bacteria are metabolically active, while particle-associated bacteria usually exhibit a larger proportion of active bacteria. Most previous studies on the active fraction of bacteria focus on planktonic communities, and there are only a few studies on sediment an...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-002-2005-0
更新日期:2002-03-01 00:00:00
abstract::Menez Gwen, Lucky Strike and Rainbow are the three most visited and well-known deep-sea hydrothermal vent fields in the Azores region, located in the Mid-Atlantic Ridge. Their distinct geological and ecological features allow them to support a diversity of vent communities, which are largely dependent on Bacteria and ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-017-0943-9
更新日期:2017-08-01 00:00:00
abstract::Bulbous rush (Juncus bulbosus) is a pioneer species in acidic, iron-rich, coal mining lakes in the eastern part of Germany. Juncus roots are coated with iron plaques, and it has been suggested that microbial processes under the iron plaques might be supportive for Juncus plant growth. The objectives of this work were ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-002-1054-8
更新日期:2003-10-01 00:00:00
abstract::Non-trophic interactions are increasingly recognised as a key parameter of predator-prey interactions. In soil, predation by bacterivorous nematodes is a major selective pressure shaping soil bacterial communities, and many bacteria have evolved defence mechanisms such as toxicity. In this study, we show that extracel...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-011-9821-z
更新日期:2011-05-01 00:00:00
abstract::Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Gr...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-010-9770-y
更新日期:2011-04-01 00:00:00
abstract::Sediment input to the Illinois River has drastically decreased river depth and reduced habitats for aquatic organisms. Dredging is being used to remove sediment from the Illinois River, and the dredged sediment is being applied to the surface of a brownfield site in Chicago with the goal of revegetating the site. In o...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-008-9410-y
更新日期:2009-01-01 00:00:00
abstract::The hypolithic microbial community associated with quartz pavement at a high-altitude tundra location in central Tibet is described. A small-scale ecological survey indicated that 36% of quartz rocks were colonized. Community profiling using terminal restriction fragment length polymorphism revealed no significant dif...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-010-9653-2
更新日期:2010-11-01 00:00:00