Abstract:
:Many sponges host abundant and active microbial communities that may play a role in the uptake of dissolved organic matter (DOM) by the sponge holobiont, although the mechanism of DOM uptake and metabolism is uncertain. Bulk and compound-specific isotopic analysis of whole sponge, isolated sponge cells, and isolated symbiotic microbial cells of the shallow water tropical Pacific sponge Mycale grandis were used to elucidate the trophic relationships between the host sponge and its associated microbial community. δ15N and δ13C values of amino acids in M. grandis isolated sponge cells are not different from those of its bacterial symbionts. Consequently, there is no difference in trophic position of the sponge and its symbiotic microbes indicating that M. grandis sponge cell isolates do not display amino acid isotopic characteristics typical of metazoan feeding. Furthermore, both the isolated microbial and sponge cell fractions were characterized by a similarly high ΣV value-a measure of bacterial-re-synthesis of organic matter calculated from the sum of variance among individual δ15N values of trophic amino acids. These high ΣV values observed in the sponge suggest that M. grandis is not reliant on translocated photosynthate from photosymbionts or feeding on water column picoplankton, but obtains nutrition through the uptake of amino acids of bacterial origin. Our results suggest that direct assimilation of bacterially synthesized amino acids from its symbionts, either in a manner similar to translocation observed in the coral holobiont or through phagotrophic feeding, is an important if not primary pathway of amino acid acquisition for M. grandis.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Shih JL,Selph KE,Wall CB,Wallsgrove NJ,Lesser MP,Popp BNdoi
10.1007/s00248-019-01410-xsubject
Has Abstractpub_date
2020-02-01 00:00:00pages
495-510issue
2eissn
0095-3628issn
1432-184Xpii
10.1007/s00248-019-01410-xjournal_volume
79pub_type
杂志文章abstract::The original article contained mistakes in the authors' affiliations. ...
journal_title:Microbial ecology
pub_type: 已发布勘误
doi:10.1007/s00248-020-01641-3
更新日期:2020-11-20 00:00:00
abstract::The response of microbial communities to disturbance is a major concern for microbial ecologists since potential modifications in their composition and functioning may affect ecosystems to a larger extent. Microbial ecosystems may be resistant (not affected) or may present engineering (return to initial state) or ecol...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-014-0532-0
更新日期:2015-07-01 00:00:00
abstract::Assimilatory nitrate reductase gene fragments were isolated from epiphytes and plankton associated with seagrass blades collected from Tampa Bay, Florida, USA. Nitrate reductase genes from diatoms (NR) and heterotrophic bacteria (nasA) were amplified by polymerase chain reaction (PCR) using two sets of degenerate prim...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-006-9175-0
更新日期:2007-11-01 00:00:00
abstract::Geosmithia spp. (Ascomycota: Hypocreales) are little-studied, dry-spored fungi that occur in galleries built by many phloeophagous bark beetles. This study mapped the distribution and environmental preferences of Geosmithia species occurring in galleries of temperate European bark beetles. One hundred seven host tree ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-007-9251-0
更新日期:2008-01-01 00:00:00
abstract::We investigated the impact of fish farm biodeposition on benthic bacteria, thraustochytrid protists, and heterotrophic protozoa (nanoflagellates and ciliates) in an oligotrophic area of the Mediterranean Sea. The fish farm impact was investigated both on a seagrass (Posidonia oceanica) bed and on soft bottom sediments...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0117-4
更新日期:2005-08-01 00:00:00
abstract::Culturable counts of antibiotic resistant, genetically engineeredPseudomonas fluorescens were determined on antibiotic-containing plate count agar during starvation in water. Prior to starvation, colony counts obtained on all media separated into two groups. The mean of the colony counts on plate count agar with or wi...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02543883
更新日期:1990-12-01 00:00:00
abstract::Propionate degradation is a critical step during the conversion of complex organic matter under methanogenic conditions, and it requires a syntrophic cooperation between propionate-oxidizing bacteria and methanogenic archaea. Increasing evidences suggest that interspecies electron transfer for syntrophic metabolism is...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0882-x
更新日期:2017-05-01 00:00:00
abstract::In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02097402
更新日期:1988-07-01 00:00:00
abstract::Seven known species of rumen ciliates and mixedEntodinium spp. showed association with plant particles in rumen fluid in vitro. Association was greater with fresh particles than with hay, and substantially decreased when the water-soluble components of the particles were removed, suggesting that the water-soluble comp...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02015109
更新日期:1985-03-01 00:00:00
abstract::Marine Crenarchaeota represent a widespread and abundant microbial group in marine ecosystems. Here, we investigated the abundance, diversity, and distribution of planktonic Crenarchaeota in the epi-, meso-, and bathypelagic zones at three stations in the South China Sea (SCS) by analysis of crenarchaeal 16S rRNA gene...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-011-9866-z
更新日期:2011-10-01 00:00:00
abstract::Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), an uncultured α-proteobacterium, is the most destructive disease of citrus trees worldwide. In previous studies, trunk injections of penicillin reduced CLas titers and HLB symptoms in citrus. However, antibiotic effects on the whole plant microbi...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1302-1
更新日期:2019-08-01 00:00:00
abstract::Rice sheath rot has been mainly associated with the bacterial pathogen Pseudomonas fuscovaginae and in some cases to the fungal pathogen Sarocladium oryzae; it is yet unclear if they are part of a complex disease. The bacterial and fungal community associated with rice sheath rot symptomatic and asymptomatic rice plan...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01529-2
更新日期:2020-10-01 00:00:00
abstract::To elucidate the individual and multiple roles of physiological bacterial groups involved in biogeochemical cycles of carbon, nitrogen, phosphorus and sulfur, the changes in the abundance of aerobic bacteria (heterotrophs, methane oxidizers, ammonia oxidizers, sulfur oxidizers, phosphate solubilizers, phosphate accumu...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01575-w
更新日期:2021-02-01 00:00:00
abstract::Growth kinetics of 2Nitrobacter strains (N.w. and L) coexisting in the same soil are studied in batch and continuous culture. Monod's parameters are estimated numerically from experimental data in the case of the batch experiment, and from steady-state equations in the case of the chemostat. In both cases, the 2 strai...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02011417
更新日期:1984-06-01 00:00:00
abstract::Freshwater aquifers in granitic rocks are widespread microbial habitats in the terrestrial subsurface. Microbial populations in deep granitic groundwater from two recently drilled (1 and 2 years) and two old boreholes (14 and 25 years) were compared. The 16S rRNA gene sequences related to "Candidatus Magnetobacterium ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-013-0184-5
更新日期:2013-04-01 00:00:00
abstract::Although organic-aggregate-associated bacteria play a pivotal role in microbial food webs and in the cycling of major elements, their community composition and diversity have not been extensively studied, especially in shallow freshwater systems. This study is among the first to explore intra-lake horizontal heterogen...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-008-9482-8
更新日期:2009-08-01 00:00:00
abstract::Photosynthetic carbon fixation by phytoplankton is a key component of the global carbon cycle. Our understanding of the types of picoplankton and ultraphytoplankton involved in this process is evolving. However, mechanisms of regulation of photosynthetic carbon fixation in the oceans are poorly understood. All phytopl...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF00183060
更新日期:1996-11-01 00:00:00
abstract::The dynamics of carbon (C), nitrogen (N), and phosphorus (P), elemental ratios, and dark uptake/release of N and P in bacterial and phytoplankton size fractions were studied during summer 1992 in three lakes of contrasting food web structure and trophic status (L240, L110, L227). We wished to determine if phytoplankto...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF00167161
更新日期:1995-03-01 00:00:00
abstract::Biological soil crusts (BSCs) are communities of cryptogamic organisms, occurring in arid and semiarid regions all over the world. Based on both morphological identification and genetic analyses, we established a first cyanobacterial inventory using the biphasic approach for BSCs within two major biomes of southern Af...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-013-0301-5
更新日期:2014-02-01 00:00:00
abstract::Ecophysiological investigations on the salinity acclimation of the cyanobacterium Prochlorothrix hollandica SAG 10.89 led to significantly revised salinity tolerance limits. Besides potential effects of cultivation techniques, clear ion composition effects mainly explain formerly described hypersensitivity to NaCl-med...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-007-9311-5
更新日期:2008-05-01 00:00:00
abstract::Sulfate reduction activity (SRA) was measured via the radioactive tracer ((35)SO4 (=)) technique in sediment samples from the Canary Creek Marsh in Lewes, Delaware. Basal levels of SRA ranged from 130 to 319 nmoles of sulfate reduced/gram dry sediment/hour. With the exception of lactate and formate, all organic acids ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02016814
更新日期:1985-12-01 00:00:00
abstract::Studies of predator-prey systems in both aquatic and terrestrial environments have shown that grazers structure the intraspecific diversity of prey species, given that the prey populations are phenotypically variable. Populations of phytoplankton have traditionally considered comprising only low intraspecific variatio...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-013-0327-8
更新日期:2014-01-01 00:00:00
abstract::The abundance ofAeromonas hydrophila was measured monthly at 29 sites in Albemarle Sound, North Carolina and its tributaries from April 1977 through July 1979. Simultaneous measurements included heterotrophic plate count bacteria, fecal coliform bacteria, and 18 physical and chemical parameters. Using only 6 water qua...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02015127
更新日期:1983-07-01 00:00:00
abstract::The abundance, distribution, and phylogenetic diversity of members of the Fe(III)-reducing family Geobacteraceae were studied along a gradient of metal contaminants in Lake Coeur d'Alene, Idaho. Partial 16S rRNA gene fragments were amplified by PCR using primers directed toward conserved regions of the gene within the...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-005-8002-3
更新日期:2003-08-01 00:00:00
abstract::A growing body of evidence now supports the isolation of microorganisms from ancient materials. However, questions about the stringency of extraction methods and the genetic relatedness of isolated organisms to their closest living relatives continue to challenge the authenticity of these ancient life forms. Previous ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-003-2016-5
更新日期:2004-07-01 00:00:00
abstract::Bark beetles are the most important mortality agent in coniferous forests, and pheromones play important roles in their management. Dendroctonus valens LeConte was introduced from North America to China and has killed millions of healthy pines there. Trapping with semiochemicals and pheromones was deployed in D. valen...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-015-0625-4
更新日期:2015-11-01 00:00:00
abstract::Fungal immigration dynamics and community development were followed over time on sets of surface-disinfested apple leaves in the field. Immigration was defined as the arrival of viable propagules on the leaf surface. In three separate experiments (May, June, July), total numbers of fungal immigrants, numbers of filame...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02011695
更新日期:1989-07-01 00:00:00
abstract::The long-term disposal of radioactive wastes in a deep geological repository is the accepted international solution for the treatment and management of these special residues. The microbial community of the selected host rocks and engineered barriers for the deep geological repository may affect the performance and th...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-015-0630-7
更新日期:2015-11-01 00:00:00
abstract::Regeneration of nutrients in aquatic ecosystems may be affected by autolysis, bacterial degradation, and activities of phagotrophic organisms. To examine the relative importance of these processes, mineralization of carbon, nitrogen, and phosphorus was studied in a two-stage continuous culture system. In the first sta...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02010599
更新日期:1985-09-01 00:00:00
abstract::To reveal the variation of microbial community functions during water filtration process in river sediments, which has been utilized widely in natural water treatment systems, this study investigates the influence of municipal wastewater discharge to streams on the phylotype and metabolic potential of the microbiome i...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-015-0680-x
更新日期:2016-01-01 00:00:00