Abstract:
:Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 3 is involved in calcium mobilization from the endoplasmic reticulum into the cytosol and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. In order to elucidate the role of SERCA3 in colorectal carcinogenesis and subsequent progression, its expression was examined using immunohistochemistry and in situ hybridization (ISH) on tissue microarrays containing colorectal carcinomas, adjacent non-neoplastic mucosa (NNM) and adenoma, and metastatic carcinoma in lymph node and liver. Colorectal carcinoma tissue and cell lines were assessed for SERCA3 expression by western blotting or RT-PCR, respectively. SERCA3 was distinctively expressed in Colo201, Colo205, DLD-1, HCT-15, HCT-116, HT-29, KM-12, SW480, SW620 and WiDr cells at both the mRNA and protein levels. SERCA3 mRNA expression was low in carcinoma when compared to that in matched NNM by quantitative PCR (P<0.05), while the converse was true by ISH. Lower expression of SERCA3 was immunohistochemically observed in colorectal carcinoma when compared to that in NNM and adenoma (P<0.05). In contrast, primary carcinoma showed high SERCA3 expression when compared to that in metastatic carcinoma in lymph node or liver by IHC (P<0.05). Immunohistochemically, SERCA3 expression was negatively related to lymphatic invasion, but not with age, gender, depth of invasion, venous invasion, lymph node metastasis, distant metastasis, TNM stage, degree of differentiation or survival rate (P>0.05). There was a positive relationship between SERCA3 expression and serum CEA levels in the carcinoma patients (P<0.05). Cox's proportional hazards model indicated that depth of invasion and distant metastasis are independent prognostic factors for overall colorectal carcinomas (P<0.05). These findings suggest that aberrant SERCA3 expression is closely linked to the adenoma-adenocarcinoma sequence and progression of colorectal carcinomas.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Gou WF,Niu ZF,Zhao S,Takano Y,Zheng HCdoi
10.3892/or.2013.2837subject
Has Abstractpub_date
2014-01-01 00:00:00pages
232-40issue
1eissn
1021-335Xissn
1791-2431journal_volume
31pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::MicroRNAs (miRNAs) are a group of important regulators in human types of cancer including non-small cell lung cancer (NSCLC). miR-616 has been found to be a novel cancer-related miRNA. However, the expression and biological function of miR-616 in NSCLC have not been investigated. In this study, qRT-PCR was performed t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5854
更新日期:2017-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common tumors in the world. At present, the details of the mechanism responsible for HCC formation and maintenance remain unclear. However, the cancer stem cell (CSC) theory suggests that liver cancer stem cells (LCSCs) may be respon...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2012.2082
更新日期:2013-01-01 00:00:00
abstract::MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assesse...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3546
更新日期:2014-12-01 00:00:00
abstract::Inhibins are dimeric glycoproteins, composed of an α-subunit and one of two possible β-subunits (βA or βB), with substantial roles in human reproduction and in endocrine-responsive tumours. Recently a novel β subunit named βC was described, although it is still unclear if normal or cancerous cervical epithelial cells ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::The peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1β (PGC-1β) is a well-established regulator of mitochondrial biogenesis. However, the underlying mechanism of PGC-1β action remains elusive. This study reveals that knockdown of endogenous PGC-1β by short-hairpin RNA (shRNA) leads to a decrease in t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2628
更新日期:2013-10-01 00:00:00
abstract::The degradation of basement membranes by tumor cells involves secretion and activation of proteinases, such as the matrix metalloproteinases (MMPs), and results from an imbalance between their inhibitors and activators that are controlled by various growth factors or cytokines, among which TGF-β(1) may be the most int...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2128
更新日期:2013-02-01 00:00:00
abstract::Cisplatin, an important chemotherapeutic agent against testicular germ cell cancer, induces testicular toxicity on Leydig and Sertoli cells, leading to serious side-effects such as azoospermia and infertility. In a previous study, it was found that simvastatin enhanced the sensitivity of Leydig tumor cells to chemothe...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4192
更新日期:2015-10-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most malignant intracranial tumor. Although the affected patients are usually treated with surgery combined with radiotherapy and chemotherapy, the median survival time for GBM patients is still approximately 12‑14 months. Identifying the key molecular mechanisms and targets of GBM...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7204
更新日期:2019-08-01 00:00:00
abstract::Pancreatic cancer is usually diagnosed in the advanced stages and is sensitive to only few therapies. The forkhead box L1 (FOXL1) and protein phosphatase 2A (PP2A) have been recognized to be tumor suppressive in human pancreatic cancers. In the present study, we co-expressed the two tumor suppressive molecules with a ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4592
更新日期:2016-04-01 00:00:00
abstract::The aim of the present study was to discover the signaling pathways associated with 2-(3-hydroxy-5-methoxy-phenyl)-6,7-methylenedioxyquinolin-4-one (YYK1)-induced apoptosis in HL-60 human leukemia cells. YYK1 induced cytotoxic effects, cell morphological changes, decreased the cell ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1923
更新日期:2012-10-01 00:00:00
abstract::High-density single nucleotide polymorphism (SNP) array is a recently introduced technology that genotypes more than 10,000 human SNPs on a single array. It has been shown that SNP arrays can be used to determine not only SNP genotype calls, but also DNA copy number (DCN) aberrations, which are common in solid tumors....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.15.4.1057
更新日期:2006-01-01 00:00:00
abstract::Doxorubicin (Dox) is one of the most commonly used and highly effective antineoplastic agents, but the clinical application of this broad spectrum drug is largely hampered by its poor stability and serious toxicity to normal tissues. Hence, it is essential to improve the therapeutic effect and decrease the systematic ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1243
更新日期:2011-06-01 00:00:00
abstract::The aberrant expression of miR107‑5p is closely related to the development of several types of human cancers. However, the role of miR‑107‑5p in endometrial carcinoma (EC) has not been fully confirmed. In the present study, we aimed to explore the function of miR‑107‑5p in EC carcinogenesis. EC samples and normal endo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6936
更新日期:2019-03-01 00:00:00
abstract::Apart from genetic mutations, epigenetic alteration is a common phenomenon that contributes to neoplastic transformation in colorectal cancer. Transcriptional silencing of tumor-suppressor genes without changes in the DNA sequence is explained by the existence of promoter hypermethylation. To test this hypothesis, we ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3993
更新日期:2015-07-01 00:00:00
abstract::trans‑Resveratrol (Rsv) is a natural compound contained in red wine and grape skins that has various beneficial effects for organisms such as lengthening of their life span. Rsv induces expression of the human TP53 and HELB genes, which are involved in the regulation of DNA maintenance. In the present study, a lucifer...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7598
更新日期:2020-07-01 00:00:00
abstract::Patients with hepatocellular carcinoma (HCC) have a poor prognosis, and novel molecular targets for treating recurrence and progression of the disease along with associated biomarkers are urgently required. In the present study, expression and the regulatory mechanism of TUSC1 (tumor suppressor candidate 1) were inves...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2939
更新日期:2014-03-01 00:00:00
abstract::Resolvins, an endogenous lipid mediator derived from eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) of fish oil, has been reported to have anti-inflammatory and antitumor effect in various pathogenic processes. However, there are no studies about the effects of resolvin D1 and E1 on concanavalin A-induced h...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4389
更新日期:2016-01-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT) is an attractive target for cancer gene therapy. However, the poor inhibition of telomerase and a time lag between the inhibition and arrest of cell proliferation limits its use in cancer gene therapy. RNA interference (RNAi) has been proposed as a potential technique for...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::The monoterpene perillyl alcohol (POH) is a naturally occurring compound derived from citrus fruits, mint and herbs. It exhibited chemotherapeutic potential against various malignant tumors in preclinical models and is currently being tested in clinical trials in patients with refractory advanced cancers. POH inhibits...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2167
更新日期:2013-02-01 00:00:00
abstract::Few studies have examined apoptosis induced by low-voltage electric pulses (LVEPs). LVEP-induce changes in membrane potential that are below the membrane breakdown threshold and increase membrane permeability without electroporation (pore formation) through the transport of extracellular substances via phagocytosis. W...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000780
更新日期:2010-05-01 00:00:00
abstract::It is known that the TGF-β superfamily receptors act as master regulators of cancer progression. However, alteration and role of type III TGF-β receptor (TβRIII, or betaglycan) as the most abundant of the TGF-β receptor has not been explored in salivary gland adenoid cystic carcinoma (ACC). Here, we reported that tumo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4390
更新日期:2016-01-01 00:00:00
abstract::CXC chemokine receptor 7 (CXCR7) has been implicated in tumor development and metastasis in multiple malignancies. Yet, the function and molecular mechanisms of CXCR7 in human osteosarcoma (OS) are still unclear. The aim of the present study was to investigate the role of CXCR7 in human OS. The expression of CXCR7 was...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3290
更新日期:2014-09-01 00:00:00
abstract::External abdomino-pelvic irradiation after primary surgery in early stages of epithelial ovarian carcinoma has been used as adjuvant therapy. The aims of this study were to evaluate efficacy and tolerability of abdomino-pelvic radiotherapy in ovarian carcinomas and to find predictive factors for recurrent disease. Fro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::The growth of malignant tumors is associated with mechanisms of immune escape and inhibition of apoptosis. Livin is a novel member of the inhibitors of apoptosis (IAP) protein family that inhibits cell apoptosis. Livin is specifically expressed by the majority of tumor cells, but it is not expressed in normal adult ti...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2133
更新日期:2013-02-01 00:00:00
abstract::To investigate the antitumor action of arsenic trioxide (As2O3) by intratumoral injection into solid tumors, tumor growth inhibition (TGI) and angiogenesis of heterotransplanted esophageal carcinoma in mice was carried out. The cultured human esophageal carcinoma cells were inoculated into both laterals of the abdomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is known to play a central role in tumour angiogenesis. Up to now inconclusive data have been published on the clinical-biological significance of circulating VEGF and on the most suitable blood fraction for measuring it. The aims of this pilot study were to assess VEGF in blo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::Flavonoids exhibit chemopreventive and chemotherapeutic effects. Butein, a bioactive flavonoid isolated from numerous native plants, has been shown to induce apoptosis in human cancer cells. In the current study, the molecular mechanisms of butein action on cell proliferation and apoptosis of neuroblastoma cells were ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1632
更新日期:2012-04-01 00:00:00
abstract::Chemotherapy is one of the methods to treat patients with non-small cell lung cancer (NSCLC) developing resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), such as gefitinib. Previous studies revealed that the sensitivity to chemotherapy may depend on different cellular mechanisms ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6242
更新日期:2018-04-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase and mutations in this gene are major drivers of lung cancer development. EGFR tyrosine kinase inhibitors (TKIs) are standard first‑line therapies for patients with advanced non‑small cell lung cancer (NSCLC) with activating EGFR mutations, but are n...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7140
更新日期:2019-07-01 00:00:00
abstract::microRNAs are an abundant class of short endogenous non-coding RNAs that function as important regulators of multiple target genes and participate in diverse biological roles in carcinogenesis. However, the role of miR-7 in lung cancer remains unclear and requires further elucidation. In the present study, we found a ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3519
更新日期:2014-12-01 00:00:00