Abstract:
:Chemotherapy is one of the methods to treat patients with non-small cell lung cancer (NSCLC) developing resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), such as gefitinib. Previous studies revealed that the sensitivity to chemotherapy may depend on different cellular mechanisms of acquired EGFR-TKIs resistance. Thus, the present study aimed to investigate the sensitivity of distinct gefitinib-resistant NSCLC cell lines to chemotherapy in order to help select effective treatment regimens for patients with EGFR-TKI resistance. In the present study, we established two gefitinib-resistant cell lines (PC-9/ZD and PC-9/GR) with the human lung adenocarcinoma cell line PC-9 (carrying the delE746-A750 mutation in the EGFR gene). PC-9/ZD cell line expressed the T790M mutation, while PC-9/GR presented the phenotypes of epithelial to mesenchymal transition (EMT). PC-9/ZD cells were more sensitive to paclitaxel and docetaxel than PC-9 cells and knockdown of T790M decreased this sensitivity. In addition, PC-9/GR cells were less sensitive to chemotherapeutic drugs tested, including cisplatin, gemcitabine, pemetrexed, paclitaxel and docetaxel, compared to PC-9 and PC-9/ZD cells. CDH1 transfection reversed the EMT and restored the sensitivity to chemotherapy in PC-9/GR cells. Furthermore, PC-9 cells became resistant to chemotherapy after TGF-β1-induced EMT. The EMT in NSCLC cells significantly increased cancer stem cell (CSC) properties and tumorgenicity. Collectively, the present study revealed that gefitinib-resistant NSCLC cells carrying the T790M mutation were sensitive to taxane chemotherapy, indicating that T790M is a useful biomarker for the selection of chemotherapy. EMT in NSCLC cells confers resistance to chemotherapy, which may be associated with enhanced CSC properties.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Zhou J,Hu Q,Zhang X,Zheng J,Xie B,Xu Z,Zhang Wdoi
10.3892/or.2018.6242subject
Has Abstractpub_date
2018-04-01 00:00:00pages
1783-1792issue
4eissn
1021-335Xissn
1791-2431journal_volume
39pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Neuroblastoma is the most common inheritable, solid neoplasm in children found under the age of 7 and accounts for approximately 7% of childhood cancers. A common treatment that has been prescribed for over a decade is retinoid therapy [using all‑trans retinoic acid (RA)]. Treatment with this differentiating agent has...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7681
更新日期:2020-09-01 00:00:00
abstract::Although antiestrogens significantly improve the survival of patients with ER-positive breast cancer, therapeutic resistance remains a major limitation. The combinatorial use of antiestrogen with other therapies was proposed to increase their efficiency and more importantly, to prevent or delay the resistance phenomen...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4873
更新日期:2016-08-01 00:00:00
abstract::Gene expression patterns as well as gene interactions are under investigation for their involvement in tumour heterogeneity. The molecular classification of breast cancer based on hormone receptor expression, grade and HER2 receptor levels, is indicative but not adequate enough to c...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2018
更新日期:2012-12-01 00:00:00
abstract::New therapeutic strategies are needed for colorectal cancer (CRC) treatment. MicroRNAs are involved in cancer‑pertinent cellular processes, including chemoresistance. As miR‑302a is an embryonic stem cell‑specific microRNA, studies on miR‑302a have focused on its role in human stem cells. Studies analyzing miR‑302 fun...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5237
更新日期:2017-01-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) is a common non-Hodgkin lymphoma. A20 and mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) are known to be related to DLBCL pathogenesis and progression. This study aimed to assess the effects of phorbol myristate acetate/ionomycin (PMA/IONO) on the growth a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4899
更新日期:2016-08-01 00:00:00
abstract::Cancer cachexia is a life‑threatening syndrome associated with myofiber damage. Tumor factors impair muscle regeneration by promoting myoblast apoptosis. Ghrelin is a multifunctional hormone with an anti‑apoptotic effect, but its mechanism of action is not fully understood. In the present study, we investigated whethe...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6213
更新日期:2018-03-01 00:00:00
abstract::Lung cancer is the most frequent and deadliest cancer in the world, especially in China. However, the molecular mechanisms involved in lung cancer remain unclear. Occludin (OCLN), one of the first identified tight junction proteins, has been revealed to be a necessary integral protein for tight junction structure and ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6408
更新日期:2018-07-01 00:00:00
abstract::DNA intrastrand cross-linking agents such as oxaliplatin induce DNA double-strand breaks (DSBs) during DNA repair and replication. In the present study, we hypothesized that DNA intrastrand cross-linking agents may significantly benefit colorectal cancer patients with deficiencies in DSB repair. Seventy-eight patients...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4488
更新日期:2016-03-01 00:00:00
abstract::Beta1-6 branching of L-PHA reactive oligosaccharides, one of the N-glycan structures, plays an important role in the biological behavior of various tumor cell lines. We reported previously that the expression of L-PHA reactive oligosaccharides was closely associated with the prognosis of patients with human diffuse la...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The present study was carried out to investigate the clinical significance of TMPRSS3 and TNFRSF11B in breast cancer. Thus, the expression levels of TMPRSS3 and TNFRSF11B and the correlation with prognosis in patients with breast cancer were analyzed in silico using gene microarray and hierarchical clustering analysis...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5449
更新日期:2017-04-01 00:00:00
abstract::The tumor necrosis factor receptor (TNFR)-associated factor 4 (TRAF4) is a member of TRAF family proteins that act as major signal transducers of the TNF receptor and the interleukin-1 receptor/Toll-like receptor (IL-1R/TLR) superfamily. TRAF4 has been reported to be overexpressed in various human cancers. However, th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2789
更新日期:2013-12-01 00:00:00
abstract::Lung cancer is one of the most common cancers worldwide and accounts for 28% of all cancer-related deaths. The expression of the β2‑adrenergic receptor (β2‑AR), one of the stress‑inducible receptors, has been reported to be closely correlated with malignant tumors. However, the role of β2‑AR activation in human lung e...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4966
更新日期:2016-09-01 00:00:00
abstract::Nucleostemin (NS) plays an important role in tumorigenesis and progression. Most studies consider that NS plays its role through combining with p53 and inhibiting it, however our previous studies revealed that NS could also function without the existence of p53. To date, few studies have focused on the p53-independent...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3240
更新日期:2014-08-01 00:00:00
abstract::Cyclin‑dependent kinase 5 regulatory subunit‑associated protein 3 (CDK5RAP3 or C53) is involved in the development of various types of tumor, and alternative splicing of C53 results in numerous transcription variants that encode different isoforms. The present study aimed to clone human C53 isoform d (IC53d) and expl...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7042
更新日期:2019-05-01 00:00:00
abstract::Recent studies suggest that latexin (Lxn) expression is involved in stem cell regulation and that it plays significant roles in tumor cell migration and invasion. The clinicopathological significance of Lxn expression and its possible correlation with CD133 expression in pancreatic ductal adenocarcinoma (PDAC) is curr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4353
更新日期:2016-01-01 00:00:00
abstract::The development and characterization of effective anticancer drugs against colorectal cancer (CRC) is of urgent need since it is the second most common cause of cancer death. The study was designed to evaluate the effects of two IGF-1R antagonists, MK-0646, a recombinant fully humanized monoclonal antibody and OSI-906...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2819
更新日期:2014-01-01 00:00:00
abstract::Recombinant hirudin (rH) is a highly potent and specific inhibitor of thrombin, and has been shown to inhibit the growth and metastasis of several types of cancers in experimental tumor models. The objective of this study was to evaluate the antitumor effects and explore the underlying mechanisms of rH in Hep-2 human ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3717
更新日期:2015-03-01 00:00:00
abstract::MicroRNA-17-5p has been proven upregulated in many human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma is still unclear. Thus, the aim of the present study was to explore the effects of miR-17-5p in osteosarcoma tumorigenesis and development. The ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4542
更新日期:2016-03-01 00:00:00
abstract::Signaling pathways that activate mTOR (mammalian target of rapamycin) are altered in many human cancers and these alterations are associated with prognosis and treatment response. mTOR inhibition can restore sensitivity to DNA damaging agents such as cisplatin. The rapamycin derivat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1666
更新日期:2012-05-01 00:00:00
abstract::Histone acetylation is one of the key chromatin modifications that control gene transcription during development and tumorigenesis. Recently, it was reported that the histone deacetylase inhibitor, Trichostatin A (TSA), induces growth arrest and apoptosis in tumors. However, the mol...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1496
更新日期:2012-02-01 00:00:00
abstract::In order to investigate the involvement of the IER3/PP2A-B56γ/ERK-positive feedback loop, which leads to sustained phosphorylation/activation of ERK in carcinogenesis, we immunohistochemically examined the expression of IER3 and phosphorylated ERK in lung tumor tissues. IER3 was overexpressed in all cases of adenocarc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4677
更新日期:2016-05-01 00:00:00
abstract::Zinc finger protein 307 (ZNF307) is a new Krüppel-associated box zinc-finger protein gene and a member of the zinc-finger family of transcriptional factors. Notably, the role of ZNF307 and its underlying mechanisms involved in hepatocarcinogenesis are poorly investigated. In the present study, we found that the expres...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5868
更新日期:2017-10-01 00:00:00
abstract::A previous study by our group suggested that testis expressed 10 (Tex10) contributes to tumor progression by promoting stem cell‑like features in hepatocellular carcinoma. However, the relevance of pluripotency factor Tex10 in esophageal squamous cell carcinoma (ESCC) has remained elusive. The objective of the present...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7376
更新日期:2019-12-01 00:00:00
abstract::The S100A2 gene has been reported to be a putative tumor‑suppressor gene. Nevertheless, overexpression of S100A2 has been found in certain types of cancer. This study investigated S100A2 expression in tissue specimens of gastritis, intestinal metaplasia, adenomatous dysplasia and ga...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2236
更新日期:2013-04-01 00:00:00
abstract::Epithelial to mesenchymal transition (EMT) is a process implicated in cancer progression in which the underlying cellular changes have been identified mainly using in vitro models. We determined the expression of some putative EMT biomarkers including E-cadherin, beta-catenin, zinc finger factor Snail (Snail), transfo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Several investigators have demonstrated that TGF-beta stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-2...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::Glutamine RF‑amide peptide (QRFP) belongs to the RFamide neuropeptide family, which is involved in a wide spectrum of biological activities, ranging from food intake and cardiovascular functioning to analgesia, aldosterone secretion, locomotor activity and reproduction. Recently, QRFP has been demonstrated to exert it...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6893
更新日期:2019-02-01 00:00:00
abstract::The aim of this study was to assess the ability of a new magnetic resonance (MR) angiography technique that does not use contrast enhancement medium to depict the vascularity of musculoskeletal neoplasms, and evaluate its clinical utility. We performed 3D fresh blood imaging (FBI) MR angiography in 57 patients with bo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::Non‑small cell lung cancer (NSCLC) accounts for over 80% of all diagnosed lung cancer cases. Lung cancer is the leading cause of cancer‑related deaths worldwide. Most NSCLC cells overexpress vascular endothelial growth factor‑A (VEGF‑A) which plays a pivotal role in tumour angiogenesis. Anti‑angiogenic therapies inclu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7580
更新日期:2020-07-01 00:00:00
abstract::Lymph node metastases are responsible for shorter survival in oral squamous cell carcinoma (OSCC). The aim of the present study was to assess the node metastasis frequency and survival according to the node metastasis features in 121 neck dissections (NDs) performed for OSCC, identifying evidence-based correlations an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2779
更新日期:2013-12-01 00:00:00