Microbial communities in the saturated groundwater environment I: Methods of isolation and characterization of heterotrophic bacteria.

Abstract:

:In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard to 155 features. The media for biochemical differentiation are listed as well as methods of morphological discrimination. 246 strains of heterotrophic and oligotrophic bacteria, isolated from five water samples from different depths of the saturated groundwater area, were used for optimizing media and test reactions.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Kölbel-Boelke J,Tienken B,Nehrkorn A

doi

10.1007/BF02097402

subject

Has Abstract

pub_date

1988-07-01 00:00:00

pages

17-29

issue

1

eissn

0095-3628

issn

1432-184X

journal_volume

16

pub_type

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