Abstract：
:Salt marshes are unique ecosystem of which a microbial community is expected to be affected by global climate change. In this study, by using T-RFLP analysis, quantitative PCR, and pyrosequencing, we comprehensively analyzed the microbial community structure responding to elevated CO2 (eCO2) and N addition in a salt marsh ecosystem subjected to CO2 manipulation and N addition for about 3 years. We focused on the genes of microbes relevant to N-cycling (denitrification and nitrification), CH4-flux (methanogens and methanotrophs), and S-cycling (sulfate reduction) considering that they are key functional groups involved in the nutrient cycle of salt marsh system. Overall, this study suggests that (1) eCO2 and N addition affect functional microbial community involved in greenhouse gas flux in salt marsh system. Specifically, the denitrification process may be facilitated, while the methanogenesis may be impeded due to the outcompeting of sulfate reduction by eCO2 and N. This implies that future global change may cause a probable change in GHGs flux and positive feedback to global climate change in salt marsh; (2) the effect of eCO2 and N on functional group seems specific and to contrast with each other, but the effect of single factor would not be compromised but complemented by combination of two factors. (3) The response of functional groups to eCO2 and/or N may be directly or indirectly related to the plant community and its response to eCO2 and/or N. This study provides new insights into our understanding of functional microbial community responses to eCO2 and/or N addition in a C3/C4 plant mixed salt marsh system.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Lee SH,Megonigal PJ,Kang Hdoi
10.1007/s00248-017-0960-8subject
Has Abstractpub_date
2017-10-01 00:00:00pages
670-680issue
3eissn
0095-3628issn
1432-184Xpii
10.1007/s00248-017-0960-8journal_volume
74pub_type
杂志文章abstract：:Adenosine triphosphate in soil is measured by the luciferin-luciferase bioluminescence method. Cation exchange in the ATP extraction procedure is used to reduce the content of, e.g., Fe, Al, and Cations in extracts. The efficiency of the cation exchange is determined by measuring the concentration of Fe by X-ray fluor...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02010504
更新日期：1979-06-01 00:00:00
abstract：:Genetically engineeredErwinia carotovora persisted significantly longer in thermally perturbed microcosms (35 days) than in nonstressed microcosms (5 days). Decreased pressure of competitors and predators and increased nutrient availability were examined as the most probable reasons for greater vulnerability of pertur...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02543863
更新日期：1990-12-01 00:00:00
abstract：:The long-term disposal of radioactive wastes in a deep geological repository is the accepted international solution for the treatment and management of these special residues. The microbial community of the selected host rocks and engineered barriers for the deep geological repository may affect the performance and th...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0630-7
更新日期：2015-11-01 00:00:00
abstract：:Nitrogen fixation (C2H2 reduction) by epiphylls on coffee,Coffea arabica, grown in sites with different degrees of shade, was determined. Coffee leaves with nitrogen-fixing epiphylls were found in all sites in approximately equal numbers. Rates of C2H2 reduction were similar for all sites and throughout the year, aver...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02010497
更新日期：1980-12-01 00:00:00
abstract：:Pseudomonas fluorescens strains are used in agriculture as plant growth-promoting rhizobacteria (PGPR). Nontarget effects of released organisms should be analyzed prior to their large-scale use, and methods should be available to sensitively detect possible changes in the environments the organism is released to. Acco...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9489-9
更新日期：2009-05-01 00:00:00
abstract：:Several Mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. Our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering Portugal as a case study. One h...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9536-6
更新日期：2009-11-01 00:00:00
abstract：:This study investigated the relationships of time-dependent hydrological variability and selected microbial food web components. Samples were collected monthly from the Kopački Rit floodplain in Croatia, over a period of 19 months, for analysis of bacterioplankton abundance, cell size and biomass; abundance of heterot...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0016-z
更新日期：2012-07-01 00:00:00
abstract：:The composition of the soil microbiome is influenced by environmental (abiotic) variables and biological interactions (biotic factors). To determine whether the aboveground vegetation and soil physicochemical properties were the main determinant of beta-diversity and biological interaction of soil microbial community,...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-017-1050-7
更新日期：2018-02-01 00:00:00
abstract：:Arcellininids (testate amoebae) were examined from 61 surface sediment samples collected from 59 lakes in the vicinity of former gold mines, notably Giant Mine, near Yellowknife, Northwest Territories, Canada to determine their utility as bioindicators of arsenic (As), which occurs both as a byproduct of gold extracti...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0752-6
更新日期：2016-07-01 00:00:00
abstract：:Mesophilic heterotrophic, aerobic or facultatively anaerobic bacteria that grow on yeast tryptone glucose extract agar were isolated from the surface of olive leaves of 3 or 4 different ages in January, April, July, and October from 1984 to 1989. Unweighted average linkage cluster analysis on either the Jaccard coeffi...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02539143
更新日期：1991-12-01 00:00:00
abstract：:The culturable haloarchaeal diversity in a crystallizer pond from a solar saltern has been analyzed and compared with the biodiversity directly retrieved by analysis of rRNA genes amplified from the environment. Two different sets of culture conditions have been assayed: solid medium with yeast extract as carbon sourc...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s002480000069
更新日期：2001-01-01 00:00:00
abstract：:Selective nucleic acid intercalating dyes--ethidium monoazide (EMA) and propidium monoazide (PMA)--represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limita...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-010-9719-1
更新日期：2011-01-01 00:00:00
abstract：:Biofilm-produced and commercially-purified a- and b-glucosidase and alkaline phosphatase were subjected to different spectral portions of natural and artificial light and exposed to various humic substances to elucidate their impact on enzyme activities. Photochemical degradation of all enzymes occurred under differen...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-001-1023-7
更新日期：2001-12-01 00:00:00
abstract：:Methods have been explored for detection of methylotrophs in natural samples, using environmental primers based on genes involved in the tetrahydromethanopterin (H4MPT)-linked C1 transfer pathway. The underlying hypotheses were that the H4MPT-linked pathway is an ancient methylotrophy pathway, based on gene divergence...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-004-0212-6
更新日期：2004-11-01 00:00:00
abstract：:The lower respiratory tract has been previously considered sterile in a healthy state, but advances in culture-independent techniques for microbial identification and characterization have revealed that the lung harbors a diverse microbiome. Although research on the lung microbiome is increasing and important question...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-017-1068-x
更新日期：2018-02-01 00:00:00
abstract：:We studied the relationship between testate amoebae (Protozoa) communities and the depth to the water table (DWT), pH, conductivity, and microhabitat type in Sphagnum dominated peatlands of north-western Poland and built predictive (transfer function) models for inferring DWT and pH based on the testate amoebae commun...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-004-0105-8
更新日期：2005-07-01 00:00:00
abstract：:To study the fate of Pseudomonas putida DSM 3931 in an aquatic environment, cultures of the strain were released into lake water mesocosms. P. putida, bearing the TOL-plasmid, was released as a representative xenobiotic-degrading microorganism. The release was carried out in mesocosms with unamended lake water and in ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00165812
更新日期：1994-01-01 00:00:00
abstract：:To date, there is a limited understanding of the role of the airway microbiome in the early life development of respiratory diseases such as asthma, partly due to a lack of simple and minimally invasive sample collection methods. In order to characterize the baseline microbiome of the upper respiratory tract (URT) in ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-015-0663-y
更新日期：2016-01-01 00:00:00
abstract：:A culture-independent molecular phylogenetic approach was used to study prokaryotic diversity in an anoxic activated sludge from a municipal wastewater treatment plant. Two 16S rRNA gene libraries were constructed using total genomic DNA and amplified by polymerase chain reaction using primers specific for archaeal or...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-009-9632-7
更新日期：2010-08-01 00:00:00
abstract：:Members of Fusarium solani species complex (FSSC) have been known as plant, animal, and human pathogens. Nevertheless, the taxonomic status of such an important group of fungi is still very confusing and many new species as well as lineages have been elucidated recently. Unfortunately, most of the new taxa came from t...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-014-0494-2
更新日期：2015-04-01 00:00:00
abstract：:Didymosphenia geminata (Lyngbye) M. Schmidt is a stalked freshwater diatom that is expanding its range globally. In some rivers, D. geminata forms thick and expansive polysaccharide-dominated mats. Like other stalked diatoms, D. geminata cells attach to the substratum with a pad of adhesive extracellular polymeric sub...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-016-0816-7
更新日期：2016-10-01 00:00:00
abstract：:The occurrence and distribution of Azorhizobium and Rhizobium strains that induce stem nodulation of Sesbania rostrata were determined in four vegetation zones in Senegal. Based on tests with 16 Rhizobium and 10 Azorhizobium strains nodulating S. rostrata, a method was devised to distinguish among the strains. In all ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00217424
更新日期：1995-01-01 00:00:00
abstract：:The abundance, distribution, and phylogenetic diversity of members of the Fe(III)-reducing family Geobacteraceae were studied along a gradient of metal contaminants in Lake Coeur d'Alene, Idaho. Partial 16S rRNA gene fragments were amplified by PCR using primers directed toward conserved regions of the gene within the...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-005-8002-3
更新日期：2003-08-01 00:00:00
abstract：:For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture. To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-013-0329-6
更新日期：2014-01-01 00:00:00
abstract：:More than 50% of all anthropogenic N2O emissions come from the soil. Drained Histosols that are used for agricultural purposes are particularly potent sources of denitrification due to higher stocks of organic matter and fertiliser application. However, conditions that favour denitrification can vary considerably acro...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-017-0991-1
更新日期：2017-11-01 00:00:00
abstract：:Our goals were to evaluate the tolerance of mesorhizobia to acid and alkaline conditions as well as to investigate whether acid tolerance is related to the species or the origin site of the isolates. In addition, to investigate the molecular basis of acid tolerance, the expression of chaperone genes groEL and dnaKJ wa...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0098-7
更新日期：2013-01-01 00:00:00
abstract：:Bacteria attached to rock and glass surfaces were studied in streams draining a whole-tree harvested watershed (WTH) and a nonharvested (CONTROL) watershed in the Hubbard Brook Experimental Forest, New Hampshire, U.S.A. Seasonal trends in numbers of cells/cm(2), mean cell volume, cell size-frequency distribution, and ...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02018912
更新日期：1988-09-01 00:00:00
abstract：:A study was conducted to determine the diversity of 2-, 3-, and 4-chlorobenzoate (CB) degraders in two pristine soils with similar physical and chemical characteristics. Surface soils were collected from forested sites and amended with 500 microg of 2-, 3-, or 4-CB g(-1) soil. The CB levels and degrader numbers were m...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-003-1048-1
更新日期：2004-07-01 00:00:00
abstract：:The marine, free-living Stilbonematinae (Nematoda: Desmodorida) inhabit the oxygen sulfide chemocline in marine sands. They are characterized by an association with ectosymbiotic bacteria. According to their ultrastructure the bacteria are Gram-negative and form morphologically uniform coats that cover the entire body...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF00167789
更新日期：1992-11-01 00:00:00
abstract：:Genetic diversification through the emergence of variants is one of the known mechanisms enabling the adaptation of bacterial communities. We focused in this work on the adaptation of the model strain Pseudomonas putida KT2440 in association with another P. putida strain (PCL1480) recently isolated from soil to invest...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-019-01470-z
更新日期：2020-07-01 00:00:00
