Abstract:
:Our goals were to evaluate the tolerance of mesorhizobia to acid and alkaline conditions as well as to investigate whether acid tolerance is related to the species or the origin site of the isolates. In addition, to investigate the molecular basis of acid tolerance, the expression of chaperone genes groEL and dnaKJ was analyzed using acid-tolerant and sensitive mesorhizobia. Tolerance to pH 5 and 9 was evaluated in liquid medium for 98 Portuguese chickpea mesorhizobia belonging to four species clusters. All isolates showed high sensitivity to pH 9. In contrast, mesorhizobia revealed high diversity in terms of tolerance to acid stress: 35 % of the isolates were acid sensitive and 45 % were highly tolerant to pH 5 or moderately acidophilic. An association between mesorhizobia tolerance to acid conditions and the origin soil pH was found. Furthermore, significant differences between species clusters regarding tolerance to acidity were obtained. Ten isolates were used to investigate the expression levels of the chaperone genes by northern hybridization. Interestingly, most acid-tolerant isolates displayed induction of the dnaK and groESL genes upon acid shock while the sensitive ones showed repression. This study suggests that acid tolerance in mesorhizobia is related to the pH of the origin soil and to the species cluster of the isolates. Additionally, the transcriptional analysis suggests a relationship between induction of major chaperone genes and higher tolerance to acid pH in mesorhizobia. This is the first report on transcriptional analysis of the major chaperones genes in mesorhizobia under acidity, contributing to a better understanding of the molecular mechanisms of rhizobia acidity tolerance.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Brígido C,Oliveira Sdoi
10.1007/s00248-012-0098-7subject
Has Abstractpub_date
2013-01-01 00:00:00pages
145-53issue
1eissn
0095-3628issn
1432-184Xjournal_volume
65pub_type
杂志文章abstract::The marine, free-living Stilbonematinae (Nematoda: Desmodorida) inhabit the oxygen sulfide chemocline in marine sands. They are characterized by an association with ectosymbiotic bacteria. According to their ultrastructure the bacteria are Gram-negative and form morphologically uniform coats that cover the entire body...
journal_title:Microbial ecology
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pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF00167862
更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00248-013-0301-5
更新日期:2014-02-01 00:00:00
abstract::Several Mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. Our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering Portugal as a case study. One h...
journal_title:Microbial ecology
pub_type: 杂志文章
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journal_title:Microbial ecology
pub_type: 杂志文章
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journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0012-z
更新日期:2005-04-01 00:00:00
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journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02010548
更新日期:1980-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00248-010-9752-0
更新日期:2011-02-01 00:00:00
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更新日期:2009-04-01 00:00:00
abstract::Commensal bacteria influence many aspects of an organism's behaviour. However, studies on the influence of commensal bacteria in insect mate-selection are scarce. Here, we present empirical evidence that commensal bacteria mediate mate-selection in the Oriental fruit fly, Bactrocera dorsalis. Male flies were attracted...
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journal_title:Microbial ecology
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