Immunofluorescence detection of the denitrifying strain Pseudomonas stutzeri (ATCC 14405) in seawater and intertidal sediment environments.

Abstract:

:A strain-specific immunofluorescence assay for enumeration of a marine denitrifying bacterium was developed and applied in the marine environment. The polyclonal antiserum for Pseudomonas stutzeri (ATCC 14405) did not react with other pseudomonads, other heterotrophs, or autotrophic nitrifying strains. The abundance of P. stutzeri in the shallow water column of Monterey Bay was less than 0.1% of the total bacterial abundance and decreased with depth, whereas the total bacterial abundance was variable and nearly constant with depth. P. stutzeri was also detected in the sediments of a microbial mat from Tomales Bay. The relatively low contribution of P. stutzeri to the total bacterial abundance in both environments implies that it is not a major component of the heterotrophic assemblage. This conclusion appears to hold for most other strains for which specific assays have been applied in the marine environment. The isolation of several different denitrifying strains from local marine environments implies that the culturable population is quite diverse, even in the absence of different selective enrichment media. Thus, strain specific immunofluorescence is of limited use in quantifying functional groups of bacteria. Conversely, they provide specific information on the diversity of natural populations and their relation to culturable strains.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Ward BB,Cockcroft AR

doi

10.1007/BF00171890

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

233-46

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

25

pub_type

杂志文章
  • Archaeal Biodiversity in Crystallizer Ponds from a Solar Saltern: Culture versus PCR.

    abstract::The culturable haloarchaeal diversity in a crystallizer pond from a solar saltern has been analyzed and compared with the biodiversity directly retrieved by analysis of rRNA genes amplified from the environment. Two different sets of culture conditions have been assayed: solid medium with yeast extract as carbon sourc...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s002480000069

    authors: Benlloch S,Acinas SG,Antón J,López-López A,Luz SP,Rodríguez-Valera F

    更新日期:2001-01-01 00:00:00

  • Factors controlling extremely productive heterotrophic bacterial communities in shallow soda pools.

    abstract::Dilute soda lakes are among the world's most productive environments and are usually dominated by dense blooms of cyanobacteria. Up to now, there has been little information available on heterotrophic bacterial abundance, production, and their controlling factors in these ecosystems. In the present study the main envi...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-002-2041-9

    authors: Eiler A,Farnleitner AH,Zechmeister TC,Herzig A,Hurban C,Wesner W,Krachler R,Velimirov B,Kirschner AK

    更新日期:2003-07-01 00:00:00

  • Effects of whole-tree harvest on epilithic bacterial populations in headwater streams.

    abstract::Bacteria attached to rock and glass surfaces were studied in streams draining a whole-tree harvested watershed (WTH) and a nonharvested (CONTROL) watershed in the Hubbard Brook Experimental Forest, New Hampshire, U.S.A. Seasonal trends in numbers of cells/cm(2), mean cell volume, cell size-frequency distribution, and ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02018912

    authors: Haack SK,Burton T,Ulrich K

    更新日期:1988-09-01 00:00:00

  • Plant Compartments and Developmental Stages Modulate the Balance between Niche-Based and Neutral Processes in Soybean Microbiome.

    abstract::Understanding the dynamics of plant-associated microbial communities within agriculture is well documented. However, the ecological processes that assemble the plant microbiome are not well understood. This study elucidates the relative dominance of assembly processes across plant compartments (root, stem, and leaves)...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-021-01688-w

    authors: Moroenyane I,Mendes L,Tremblay J,Tripathi B,Yergeau É

    更新日期:2021-01-18 00:00:00

  • Population density-dependent metal tolerance: One possible basis and its ecological implications.

    abstract::A population density-dependent copper (Cu) resistance mechanism in a gram-negative soil bacterium, strain TDCd1, was shown to be inducible and was accompanied by changes in the protein composition of the outer membrane of the cell envelope. Characteristically, following inoculation of TDCd1 into Cu-supplemented growth...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02075807

    authors: Duxbury T,McIntyre R

    更新日期:1989-11-01 00:00:00

  • Can't take the heat: high temperature depletes bacterial endosymbionts of ants.

    abstract::Members of the ant tribe Camponotini have coevolved with Blochmannia, an obligate intracellular bacterial mutualist. This endosymbiont lives within host bacteriocyte cells that line the ant midgut, undergoes maternal transmission from host queens to offspring, and contributes to host nutrition via nitrogen recycling a...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0264-6

    authors: Fan Y,Wernegreen JJ

    更新日期:2013-10-01 00:00:00

  • Response of benthic protozoa and thraustochytrid protists to fish farm impact in seagrass (Posidonia oceanica) and soft-bottom sediments.

    abstract::We investigated the impact of fish farm biodeposition on benthic bacteria, thraustochytrid protists, and heterotrophic protozoa (nanoflagellates and ciliates) in an oligotrophic area of the Mediterranean Sea. The fish farm impact was investigated both on a seagrass (Posidonia oceanica) bed and on soft bottom sediments...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-004-0117-4

    authors: Bongiorni L,Mirto S,Pusceddu A,Danovaro R

    更新日期:2005-08-01 00:00:00

  • Quantifying Ostreid herpesvirus (OsHV-1) genome copies and expression during transmission.

    abstract::Understanding the pathogenic potential of a new pathogen strain or a known pathogen in a new locale is crucial for management of disease in both wild and farmed animals. The Ostreid herpesvirus-1 (OsHV-1), a known pathogen of early-life-stage Pacific oysters, Crassostrea gigas, has been associated with mortalities of ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-9937-1

    authors: Burge CA,Friedman CS

    更新日期:2012-04-01 00:00:00

  • Hypolithic cyanobacteria, dry limit of photosynthesis, and microbial ecology in the hyperarid Atacama Desert.

    abstract::The occurrence of hypolithic cyanobacteria colonizing translucent stones was quantified along the aridity gradient in the Atacama Desert in Chile, from less arid areas to the hyperarid core where photosynthetic life and thus primary production reach their limits. As mean rainfall declines from 21 to

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9055-7

    authors: Warren-Rhodes KA,Rhodes KL,Pointing SB,Ewing SA,Lacap DC,Gómez-Silva B,Amundson R,Friedmann EI,McKay CP

    更新日期:2006-10-01 00:00:00

  • Wild Bee Pollen Usage and Microbial Communities Co-vary Across Landscapes.

    abstract::Bees forage for pollen and nectar at flowers but simultaneously acquire pathogenic, commensal, and likely beneficial microbes from these same flowers. Characterizing pollen usage of wild bees is therefore crucial to their conservation yet remains a challenging task. To understand pollen usage across landscapes and how...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1232-y

    authors: McFrederick QS,Rehan SM

    更新日期:2019-02-01 00:00:00

  • Comparisons of the fungal and protistan communities among different marine sponge holobionts by pyrosequencing.

    abstract::To date, the knowledge of eukaryotic communities associated with sponges remains limited compared with prokaryotic communities. In a manner similar to prokaryotes, it could be hypothesized that sponge holobionts have phylogenetically diverse eukaryotic symbionts, and the eukaryotic community structures in different sp...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-014-0393-6

    authors: He L,Liu F,Karuppiah V,Ren Y,Li Z

    更新日期:2014-05-01 00:00:00

  • Effect of several environmental parameters on carbon metabolism in histosols.

    abstract::High specific activity(14)C-labeled glucose, succinate, acetate, salicylate, and amino acids were used to examine carbon metabolism by the microbial community of Pahokee muck (aLithic medisaprist), a drained, cultivated soil of the Florida Everglades. Variations in carbon oxidation were observed from the end of the we...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02020340

    authors: Tate RL 3rd

    更新日期:1980-12-01 00:00:00

  • Dynamics of seawater bacterial communities in a shellfish hatchery.

    abstract::Bacterial disease is a significant issue for larviculture of several species of shellfish, including oysters. One source of bacteria is the seawater used throughout the hatchery. In this study carried out at a commercial oyster hatchery in Tasmania, Australia, the diversity of the bacterial community and its relations...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0183-6

    authors: Powell SM,Chapman CC,Bermudes M,Tamplin ML

    更新日期:2013-08-01 00:00:00

  • Detecting Associations Between Ciliated Protists and Prokaryotes with Culture-Independent Single-Cell Microbiomics: a Proof-of-Concept Study.

    abstract::Symbioses between prokaryotes and microbial eukaryotes, particularly ciliated protists, have been studied for a long time. Nevertheless, researchers have focused only on a few host genera and species, mainly due to difficulties in cultivating the hosts, and usually have considered a single symbiont at a time. Here, we...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1279-9

    authors: Rossi A,Bellone A,Fokin SI,Boscaro V,Vannini C

    更新日期:2019-07-01 00:00:00

  • Salmonella from a Microtidal Estuary Are Capable of Invading Human Intestinal Cell Lines.

    abstract::Faecal contamination poses health risks for the recreational users of urban estuaries. However, our understanding of the potential pathogenicity of faecal microbes in these environments is limited. To this end, a study was conducted to understand the spatial and seasonal distribution of Salmonella in water and sedimen...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01419-2

    authors: Siddiqee MH,Henry R,Deletic A,Bulach DM,Coleman RA,McCarthy DT

    更新日期:2020-02-01 00:00:00

  • Response of microbial populations to environmental disturbance.

    abstract::Taxonomic and genetic diversities of microbial communities disturbed by chemical pollutants were lower than in undisturbed reference communities. The dominant populations within the disturbed communities had enhanced physiological tolerances and substrate utilization capabilities, indicating that generalized physiolog...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02540227

    authors: Atlas RM,Horowitz A,Krichevsky M,Bej AK

    更新日期:1991-12-01 00:00:00

  • Global Networks of Symbiodinium-Bacteria Within the Coral Holobiont.

    abstract::Scleractinian corals form the framework of coral reefs and host abundant and diverse microbial communities that are fundamental to their success. A very limited number of studies have examined the co-occurrence of multiple partners within the coral 'holobiont' and their pattern of specificity over different geographic...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1255-4

    authors: Bernasconi R,Stat M,Koenders A,Huggett MJ

    更新日期:2019-04-01 00:00:00

  • Litter Quality Modulates Effects of Dissolved Nitrogen on Leaf Decomposition by Stream Microbial Communities.

    abstract::Rates of leaf litter decomposition in streams are strongly influenced both by inorganic nutrients dissolved in stream water and by litter traits such as lignin, nitrogen (N) and phosphorus (P) concentrations. As a result, decomposition rates of different leaf species can show contrasting responses to stream nutrient e...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-019-01353-3

    authors: Jabiol J,Lecerf A,Lamothe S,Gessner MO,Chauvet E

    更新日期:2019-05-01 00:00:00

  • PAH degradation capacity of soil microbial communities--does it depend on PAH exposure?

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the environment. But is their microbial degradation equally wide in distribution? We estimated the PAH degradation capacity of 13 soils ranging from pristine locations (total PAHs approximately 0.1 mg kg(-1)) to heavily polluted industrial sites (tot...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-005-0022-5

    authors: Johnsen AR,Karlson U

    更新日期:2005-11-01 00:00:00

  • The Bacterial Community of the Foliose Macro-lichen Peltigera frigida Is More than a Mere Extension of the Microbiota of the Subjacent Substrate.

    abstract::Lichens host highly diverse microbial communities, with bacteria being one of the most explored groups in terms of their diversity and functioning. These bacteria could partly originate from symbiotic propagules developed by many lichens and, perhaps more commonly and depending on environmental conditions, from differ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-020-01662-y

    authors: Leiva D,Fernández-Mendoza F,Acevedo J,Carú M,Grube M,Orlando J

    更新日期:2021-01-06 00:00:00

  • Multiple, novel biologically active endophytic actinomycetes isolated from upper Amazonian rainforests.

    abstract::Microbial biodiversity provides an increasingly important source of medically and industrially useful compounds. We have isolated 14 actinomycete species from a collection of approximately 300 plant stem samples from the upper Amazonian rainforest in Peru. All of the cultured isolates produce substances with inhibitor...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9494-z

    authors: Bascom-Slack CA,Ma C,Moore E,Babbs B,Fenn K,Greene JS,Hann BD,Keehner J,Kelley-Swift EG,Kembaiyan V,Lee SJ,Li P,Light DY,Lin EH,Schorn MA,Vekhter D,Boulanger LA,Hess WM,Vargas PN,Strobel GA,Strobel SA

    更新日期:2009-08-01 00:00:00

  • Altering the Gut Microbiome of Cattle: Considerations of Host-Microbiome Interactions for Persistent Microbiome Manipulation.

    abstract::The beef cattle industry represents a significant portion of the USA's agricultural sect, with beef cattle accounting for the most red meat consumed in the USA. Feed represents the largest input cost in the beef industry, accounting for approximately 70% of total input cost. Given that, novel methods need to be employ...

    journal_title:Microbial ecology

    pub_type: 杂志文章,评审

    doi:10.1007/s00248-018-1234-9

    authors: Clemmons BA,Voy BH,Myer PR

    更新日期:2019-02-01 00:00:00

  • An evaluation of 18S rDNA approaches for the study of fungal diversity in grassland soils.

    abstract::Fungal community structure and diversity in two types of agricultural grassland soil were investigated by amplified 18S ribosomal DNA restriction analysis (ARDRA) and 18S ribosomal DNA sequence analysis. These two grassland sites represent a species-rich old hay meadow and an agriculturally improved site with low flor...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-003-2018-3

    authors: Hunt J,Boddy L,Randerson PF,Rogers HJ

    更新日期:2004-05-01 00:00:00

  • Symbiotic relationships of legumes and nodule bacteria on Barro Colorado Island, Panama: a review.

    abstract::Abstract New data on 129 bacterial isolates were analyzed together with prior samples to characterize community-level patterns of legume-rhizobial symbiosis on Barro Colorado Island (BCI), Panama. Nodules have been sampled from 24 BCI legume species in 18 genera, representing about one quarter of the legume species an...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-007-9309-z

    authors: Parker MA

    更新日期:2008-05-01 00:00:00

  • A Comparison of the Survival of Intraperiplasmic and Attack Phase Bdellovibrios with Reduced Oxygen

    abstract::The ability of intraperiplasmic and attack phase bdellovibrios to survive and/or grow under anoxic and microaerobic conditions was examined. Both halotolerant and nonhalotolerant bdellovibrio strains were examined. In all instances, the bdellovibrio strains were unable to grow under anoxic conditions, but were able to...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00170105

    authors: Schoeffield AJ,Williams HN,Turng B,Fackler WA Jr

    更新日期:1996-07-01 00:00:00

  • Response of the sulfate-reducing community to the re-establishment of estuarine conditions in two contrasting soils: a mesocosm approach.

    abstract::We studied the response of the sulfate-reducing prokaryote (SRP) communities to the experimental variation of salinity and tide in an outdoor mesocosm setup. Intact soil monoliths were collected at two areas of the Haringvliet lagoon (The Netherlands): one sampling location consisted of agricultural grassland, drained...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9614-9

    authors: Miletto M,Loeb R,Antheunisse AM,Bodelier PL,Laanbroek HJ

    更新日期:2010-01-01 00:00:00

  • Abundance, diversity, and dynamics of viruses on microorganisms in activated sludge processes.

    abstract::We examined the abundance of viruses on microorganisms in activated sludge and the dynamics of their community structure. Direct counting with epifluorescence microscopy and pulsed-field gel electrophoresis (PFGE) were applied to 20 samples from 14 full-scale wastewater treatment plants (wwtps) treating municipal, ind...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9150-9

    authors: Otawa K,Lee SH,Yamazoe A,Onuki M,Satoh H,Mino T

    更新日期:2007-01-01 00:00:00

  • Drying-rewetting cycles affect fungal and bacterial growth differently in an arable soil.

    abstract::Drying and rewetting is a frequent physiological stress for soil microbial communities; a stress that is predicted to grow more influential with future climate change. We investigated the effect of repeated drying-rewetting cycles on bacterial (leucine incorporation) and fungal (acetate in ergosterol incorporation) gr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9723-5

    authors: Bapiri A,Bååth E,Rousk J

    更新日期:2010-08-01 00:00:00

  • How do Elevated CO2 and Nitrogen Addition Affect Functional Microbial Community Involved in Greenhouse Gas Flux in Salt Marsh System.

    abstract::Salt marshes are unique ecosystem of which a microbial community is expected to be affected by global climate change. In this study, by using T-RFLP analysis, quantitative PCR, and pyrosequencing, we comprehensively analyzed the microbial community structure responding to elevated CO2 (eCO2) and N addition in a salt m...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-017-0960-8

    authors: Lee SH,Megonigal PJ,Kang H

    更新日期:2017-10-01 00:00:00

  • Nitrogen economy of endolithic microbial communities in hot and cold deserts.

    abstract::The source of combined nitrogen in endolithic microbial communities was studied in samples from desert localities in North and South America, the Middle East, South Africa, and Antarctica. Nitrogen fixation (acetylene reduction) seems to occur only exceptionally. Evidence suggests that, in general, the nitrogen source...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02010548

    authors: Friedmann EI,Kibler AP

    更新日期:1980-06-01 00:00:00