Abstract:
:Insulin resistance (IR) is associated with elevated plasma levels of triglyceride-rich lipoproteins (TRLs) of intestinal origin. However, the mechanisms underlying the overaccumulation of apolipoprotein (apo)B-48-containing TRLs in individuals with IR are not yet fully understood. This study examined the relationships between apoB-48-containing TRL kinetics and the expression of key intestinal genes and proteins involved in lipid/lipoprotein metabolism in 14 obese nondiabetic men with IR compared with 10 insulin-sensitive (IS) men matched for waist circumference. The in vivo kinetics of TRL apoB-48 were assessed using a primed-constant infusion of L-[5,5,5-D₃]leucine for 12 h with the participants in a constantly fed state. The expression of key intestinal genes and proteins involved in lipid/lipoprotein metabolism was assessed by performing real-time PCR quantification and LC-MS/MS on duodenal biopsy specimens. The TRL apoB-48 pool size and production rate were 102% (P < 0.0001) and 87% (P = 0.01) greater, respectively, in the men with IR versus the IS men. On the other hand, intestinal mRNA levels of sterol regulatory element binding factor-2, hepatocyte nuclear factor-4α, and microsomal triglyceride transfer protein were significantly lower in the men with IR than in the IS men. These data indicate that IR is associated with intestinal overproduction of lipoproteins and significant downregulation of key intestinal genes involved in lipid/lipoprotein metabolism.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Couture P,Tremblay AJ,Kelly I,Lemelin V,Droit A,Lamarche Bdoi
10.1194/jlr.M040071subject
Has Abstractpub_date
2014-01-01 00:00:00pages
128-37issue
1eissn
0022-2275issn
1539-7262pii
jlr.M040071journal_volume
55pub_type
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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更新日期:1970-03-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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更新日期:1978-07-01 00:00:00
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journal_title:Journal of lipid research
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更新日期:1973-05-01 00:00:00
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journal_title:Journal of lipid research
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更新日期:1979-07-01 00:00:00
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journal_title:Journal of lipid research
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doi:
更新日期:1980-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-07-01 00:00:00
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更新日期:1976-11-01 00:00:00
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
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