Abstract:
:Chondrocyte apoptosis contributes to the disruption of cartilage integrity in osteoarthritis (OA). Recently, we reported that activation of volume-sensitive Cl- current (ICl,vol) mediates cell shrinkage, triggering apoptosis in rabbit articular chondrocytes. A cyclooxygenase (COX) blocker is frequently used for the treatment of OA. In the present study, we examined in vitro effects of selective blockers of COX on the TNFα-induced activation of ICl,vol in rabbit chondrocytes using the patch-clamp technique. Exposure of isolated chondrocytes to TNFα resulted in an obvious increase in membrane Cl- conductance. The TNFα-evoked Cl- current exhibited electrophysiological and pharmacological properties similar to those of ICl,vol. Pretreatment of cells with selective COX-2 blocker etodolac markedly inhibited ICl,vol activation by TNFα as well as subsequent apoptotic events such as apoptotic cell volume decrease (AVD) and elevation of caspase-3/7 activity. In contrast, a COX-1 blocker had no effect on the decrease in cell volume or the increase in caspase-3/7 activity induced by TNFα. Thus, the COX-2-selective blocker had an inhibitory effect on TNFα-induced apoptotic events, which suggests that this drug would have efficacy for the treatment of OA.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kumagai K,Kubo M,Imai S,Toyoda F,Maeda T,Okumura N,Matsuura H,Matsusue Ydoi
10.3390/ijms141019705subject
Has Abstractpub_date
2013-09-30 00:00:00pages
19705-15issue
10issn
1422-0067pii
ijms141019705journal_volume
14pub_type
杂志文章abstract::The overexpressing ABCB1 transporter is one of the key factors leading to multidrug resistance (MDR). Thus, many ABCB1 inhibitors have been found to be able to overcome ABCB1-mediated MDR. However, some inhibitors also work as a substrate of ABCB1, which indicates that in order to achieve an effective reversal dosage,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041387
更新日期:2020-02-19 00:00:00
abstract::Pulmonary vascular remodeling is a pathophysiological feature that common to all classes of pulmonary hypertension (PH) and right ventricular dysfunction, which is the major prognosis-limiting factor. Vascular, as well as cardiac tissue remodeling are associated with a re-expression of fetal variants of cellular adhes...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112371
更新日期:2017-11-08 00:00:00
abstract::The specific rates of solvolysis of neopentyl chloroformate (1) have been determined in 21 pure and binary solvents at 45.0 °C. In most solvents the values are essentially identical to those for ethyl and n-propyl chloroformates. However, in aqueous-1,1,1,3,3,3-hexafluoro-2-propanol mixtures (HFIP) rich in fluoroalcoh...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12021161
更新日期:2011-02-15 00:00:00
abstract::Due to a typesetting error during layout, several references were incorrectly listed in [...]. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,已发布勘误
doi:10.3390/ijms20112651
更新日期:2019-05-29 00:00:00
abstract::Gomisin N (GN) is lignin derived from Schisandra chinensis that has been reported to exhibit hepato-protective, anti-cancer, and anti-inflammatory effects. However, its role in whole-body energetic homeostasis remains unclear. In this study, we employed Drosophila melanogaster as a diet-induced obese model to elucidat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197209
更新日期:2020-09-29 00:00:00
abstract::Carvacrol is a natural compound known to be a highly effective antibacterial; however, it is a hydrophobic molecule, which is a limitation to its use within food packaging. Flaxseed gum (FG) films containing different contents of carvacrol (C) were produced by a film-casting method with sonication. The effects of sonic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051637
更新日期:2020-02-28 00:00:00
abstract::Monocyte chemoattractant protein-1 (MCP-1) is a well-known chemokine critically involved in the pathophysiological progression of several inflammatory diseases including arthrosclerosis. N-caffeoyltryptamine is a phenolic amide with strong anti-inflammatory effects. Therefore, in this paper, the potential effect of N-...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061148
更新日期:2017-05-27 00:00:00
abstract::Oxidative damage (production and localization of reactive oxygen species) and related response mechanisms (activity of antioxidant enzymes), and induction of Heat Shock Protein 70 expression, have been studied in the toxi-tolerant liverwort Conocephalum conicum (Marchantiales) in response to cadmium stress using two c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21186485
更新日期:2020-09-04 00:00:00
abstract::Tyrosinase (TYR) is a metalloenzyme classified as a type-3 copper protein, which is involved in the synthesis of melanin through a catalytic process beginning with the conversion of the amino acid l-Tyrosine (l-Tyr) to l-3,4-dihydroxyphenylalanine (l-DOPA). It plays an important role in the mechanism of melanogenesis ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134783
更新日期:2020-07-06 00:00:00
abstract::Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14023395
更新日期:2013-02-06 00:00:00
abstract::Attributed to the tropism for host microvascular endothelium lining the blood vessels, vascular inflammation and dysfunction represent salient features of rickettsial pathogenesis, yet the details of fundamentally important pathogen interactions with host endothelial cells (ECs) as the primary targets of infection rem...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197179
更新日期:2020-09-29 00:00:00
abstract::The MP2 and DFT/B3LYP methods at 6-311++G(d,p) and aug-cc-pdz basis sets have been used to probe the origin of relative stability preference for eclipsed acetaldehyde over its bisected counterpart. A relative energy stability range of 1.02 to 1.20 kcal/mol, in favor of the eclipsed conformer, was found and discussed. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131115360
更新日期:2012-11-20 00:00:00
abstract::Reactive oxygen species (ROS) are not only harmful to cell survival but also essential to cell signaling through cysteine-based redox switches. In fact, ROS triggers the potential activation of mitogen-activated protein kinases (MAPKs). The 90 kDa ribosomal S6 kinase 1 (RSK1), one of the downstream mediators of the MA...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228750
更新日期:2020-11-19 00:00:00
abstract::Circulating tumour cells (CTCs) from liquid biopsies are under current investigation in several cancers, including epithelial ovarian cancer (EOC) but face significant drawbacks in terms of non-standardised methodology, low viable cell numbers and accuracy of CTC identification. In this pilot study, we report that che...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134813
更新日期:2020-07-07 00:00:00
abstract::Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Phy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041417
更新日期:2020-02-19 00:00:00
abstract::Chorea acanthocytosis (ChAc), an ultra-rare devastating neurodegenerative disease, is caused by mutations in the VPS13A gene, which encodes for the protein chorein. Affected patients suffer from chorea, orofacial dyskinesia, epilepsy, parkinsonism as well as peripheral neuropathy. Although medium spinal neurons of the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051797
更新日期:2020-03-05 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has emerged as an important player in asthma control. AhR is responsive to environmental molecules and endogenous or dietary metabolites and regulates innate and adaptive immune responses. Binding of this receptor by different ligands ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228797
更新日期:2020-11-20 00:00:00
abstract::Heterosis has been widely accepted as an effective strategy to increase yields in plant breeding. Notably, the chemical hybridization agent SQ-1 induces male sterility in wheat, representing a critical potential tool in hybrid seed production. However, the mechanisms underlying the male sterility induced by SQ-1 still...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072468
更新日期:2020-04-02 00:00:00
abstract::Molecular chaperones are key components of the protein homeostasis system against protein misfolding and aggregation. It has been recently shown that these molecules can be rationally modified to have an enhanced activity against specific amyloidogenic substrates. The resulting molecular chaperone variants can be effe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051443
更新日期:2018-05-12 00:00:00
abstract::Aminolevulinic acid (ALA) is the first metabolite in the heme biosynthesis pathway in humans. In addition to the end product heme, this pathway also produces other porphyrin metabolites. Protoporphyrin (PpIX) is one heme precursor porphyrin with good fluorescence and photosensitizing activity. Because tumors and other...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms161025865
更新日期:2015-10-28 00:00:00
abstract::Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects me...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082701
更新日期:2020-04-13 00:00:00
abstract::Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the struct...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19102912
更新日期:2018-09-25 00:00:00
abstract::With the aim of contributing to the knowledge about their potential therapeutic activity, we determined the biological activities of cyanidin and its selected O-glycosides in relation to erythrocytes (RBCs) and human dermal vascular endothelial cells (HMEC-1). Furthermore, on the basis of changes in the physical/funct...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081928
更新日期:2019-04-19 00:00:00
abstract::Obesity is a worldwide epidemic problem and correlates to varieties of acute or chronic lung diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary disease, and pulmonary fibrosis. An increase of leptin, a kind of adipokine, in lean mice plasma has been found to impair immune responses and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051078
更新日期:2019-03-02 00:00:00
abstract::In plants, microRNAs have been indicated to control various developmental processes, including somatic embryogenesis (SE), which is triggered in the in vitro cultured somatic cells of plants. Although a transcriptomic analysis has indicated that numerous MIRNAs are differentially expressed in the SE of different plant...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205221
更新日期:2019-10-21 00:00:00
abstract::A series of novel 1-(4-substitutedbenzoyl)-4-(4-chlorobenzhydryl)piperazine derivatives 5a-g was designed by a nucleophilic substitution reaction of 1-(4-chlorobenzhydryl)piperazine with various benzoyl chlorides and characterized by elemental analyses, IR and (1)H nuclear magnetic resonance spectra. Cytotoxicity of t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13078071
更新日期:2012-01-01 00:00:00
abstract::Cardamom is a popular spice that has been commonly used in cuisines for flavor since ancient times. It has copious health benefits such as improving digestion, stimulating metabolism, and exhibits antioxidant and anti-inflammatory effects. The current study investigated the effect of cardamom on hemodynamic, biochemic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161126040
更新日期:2015-11-17 00:00:00
abstract::Creatine is a metabolite important for cellular energy homeostasis as it provides spatio-temporal adenosine triphosphate (ATP) buffering for cells with fluctuating energy demands. Here, we examined whether placental creatine metabolism was altered in cases of early-onset pre-eclampsia (PE), a condition known to cause ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030806
更新日期:2020-01-26 00:00:00
abstract::Hepatocyte growth factor (HGF) is an endogenously expressed bioactive substance that has a strong anti-apoptotic effect. In this study, we biochemically and histologically characterized the effects of rh-HGF on in vitro human hepatocyte injury and mouse acute liver failure (ALF) models, both of which were induced by a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081821
更新日期:2019-04-12 00:00:00
abstract::The mechanisms behind fatigue intensification during cancer therapy remain elusive. The interferon alpha-inducible protein 27 (IFI27) was the most up-regulated gene based on our previous microarray data in fatigued men with non-metastatic prostate cancer receiving localized external beam radiation therapy (EBRT). The ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140816943
更新日期:2013-08-16 00:00:00