Abstract:
:Creatine is a metabolite important for cellular energy homeostasis as it provides spatio-temporal adenosine triphosphate (ATP) buffering for cells with fluctuating energy demands. Here, we examined whether placental creatine metabolism was altered in cases of early-onset pre-eclampsia (PE), a condition known to cause placental metabolic dysfunction. We studied third trimester human placentae collected between 27-40 weeks' gestation from women with early-onset PE (n = 20) and gestation-matched normotensive control pregnancies (n = 20). Placental total creatine and creatine precursor guanidinoacetate (GAA) content were measured. mRNA expression of the creatine synthesizing enzymes arginine:glycine aminotransferase (GATM) and guanidinoacetate methyltransferase (GAMT), the creatine transporter (SLC6A8), and the creatine kinases (mitochondrial CKMT1A & cytosolic BBCK) was assessed. Placental protein levels of arginine:glycine aminotransferase (AGAT), GAMT, CKMT1A and BBCK were also determined. Key findings; total creatine content of PE placentae was 38% higher than controls (p < 0.01). mRNA expression of GATM (p < 0.001), GAMT (p < 0.001), SLC6A8 (p = 0.021) and BBCK (p < 0.001) was also elevated in PE placentae. No differences in GAA content, nor protein levels of AGAT, GAMT, BBCK or CKMT1A were observed between cohorts. Advancing gestation and birth weight were associated with a down-regulation in placental GATM mRNA expression, and a reduction in GAA content, in control placentae. These relationships were absent in PE cases. Our results suggest PE placentae may have an ongoing reliance on the creatine kinase circuit for maintenance of cellular energetics with increased total creatine content and transcriptional changes to creatine synthesizing enzymes and the creatine transporter. Understanding the functional consequences of these changes warrants further investigation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Ellery SJ,Murthi P,Gatta PAD,May AK,Davies-Tuck ML,Kowalski GM,Callahan DL,Bruce CR,Wallace EM,Walker DW,Dickinson H,Snow RJdoi
10.3390/ijms21030806subject
Has Abstractpub_date
2020-01-26 00:00:00issue
3issn
1422-0067pii
ijms21030806journal_volume
21pub_type
杂志文章abstract::Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. However, their calcium-binding domain is restricted by calmodulin from metazoans. In this study, we developed red GECI, called FRCaMP, using calmo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010111
更新日期:2020-12-24 00:00:00
abstract::Carbonic anhydrase II (CAII) is a zinc-containing metalloenzyme whose aberrant activity is associated with various diseases such as glaucoma, osteoporosis, and different types of tumors; therefore, the development of CAII inhibitors, which can represent promising therapeutic agents for the treatment of these pathologi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19071851
更新日期:2018-06-23 00:00:00
abstract::Background: To evaluate the distribution of the transforming growth factor-beta induced (TGFBI) corneal dystrophies in a multi-ethnic population in Singapore, and to present the different phenotypes with the same genotype. Methods: This study included 32 patients. Slit lamp biomicroscopy was performed for each patient...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031230
更新日期:2021-01-27 00:00:00
abstract::Pneumonia is a cause of high morbidity and mortality in humans. Animal models are indispensable to investigate the complex cellular interactions during lung injury and repair in vivo. The time sequence of lesion development and regeneration is described after endobronchial inoculation of calves with Chlamydia psittaci...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082817
更新日期:2020-04-17 00:00:00
abstract::Metabolic syndrome (MetS) is established as the combination of central obesity and different metabolic disturbances, such as insulin resistance, hypertension and dyslipidemia. This cluster of factors affects approximately 10%-50% of adults worldwide and the prevalence has been increasing in epidemic proportions over t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17111877
更新日期:2016-11-10 00:00:00
abstract::(1) Background: A high incidence of intervening sequence (IVS)4+919 G>A mutation with later-onset cardiac phenotype have been reported in a majority of Taiwan Fabry cohorts. Some evidence indicated that conventional biomarkers failed to predict the long-term progression and therapeutic outcome; (2) Methods: In this st...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051480
更新日期:2018-05-16 00:00:00
abstract::The gadolinium-based nanoagent named AGuIX® is a unique radiosensitizer and contrast agent which improves the performance of radiotherapy and medical imaging. Currently tested in clinical trials, AGuIX® is administrated to patients via intravenous injection. The presence of nanoparticles in the blood stream may induce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134673
更新日期:2020-06-30 00:00:00
abstract::A conformational analysis of nine macrocyclic thioether musks has been carried out using molecular mechanics (MMFF), density functional theory (DFT) using both B3LYP and M06 functionals, as well as Hartree-Fock and post-Hartree-Fock (MP2) ab initio methods. 6-Thia-, 10-thia- and 4-methyl-5-thia-14-tetradecananolide, 4...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10083488
更新日期:2009-08-04 00:00:00
abstract::Administration of antioxidants and anti-inflammatory agents is an effective strategy for preventing ultraviolet (UV) irradiation-induced skin damage. Artocarpus communis possesses several pharmacological activities, such as antioxidant, anticancer and anti-inflammation. However, the photoprotective activity of methan...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14023860
更新日期:2013-02-12 00:00:00
abstract::Recessively-inherited NR2E3 gene mutations cause an unusual retinopathy with abnormally-increased short-wavelength sensitive cone (S-cone) function, in addition to reduced rod and long/middle-wavelength sensitive cone (L/M-cone) function. Progress toward clinical trials to treat patients with this otherwise incurable ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102497
更新日期:2019-05-21 00:00:00
abstract::The synergistic anticancer effect of docetaxel (DTX) and curcumin (CCM) has emerged as an attractive therapeutic candidate for lung cancer treatment. However, the lack of optimal bioavailability because of high toxicity, low stability, and poor solubility has limited their clinical success. Given this, an aerosolized ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124357
更新日期:2020-06-19 00:00:00
abstract::Carbonic anhydrase (CA) is a widespread metalloenzyme playing a pivotal role in several physiological processes. Many studies have demonstrated the in vitro and in vivo sensitivity of CA to the exposure to several classes of pesticides in both humans and wildlife. This review aims to analyze and to discuss the literat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21103562
更新日期:2020-05-18 00:00:00
abstract::Flower development is a vital developmental process in the life cycle of woody perennials, especially fruit trees. Herein, we used transcriptomic, proteomic, and hormone analyses to investigate the key candidate genes/proteins in loquat (Eriobotrya japonica) at the stages of flower bud differentiation (FBD), floral bu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21145107
更新日期:2020-07-20 00:00:00
abstract::Obesity is an important aspect of the metabolic syndrome and is often associated with chronic inflammation. In this context, inflammation of organs participating in energy homeostasis (such as liver, adipose tissue, muscle and pancreas) leads to the recruitment and activation of macrophages, which secrete pro-inflamma...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22010330
更新日期:2020-12-30 00:00:00
abstract::Biotic stresses do damage to the growth and development of plants, and yield losses for some crops. Confronted with microbial infections, plants have evolved multiple defense mechanisms, which play important roles in the never-ending molecular arms race of plant⁻pathogen interactions. The complicated defense systems i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20020335
更新日期:2019-01-15 00:00:00
abstract::While drug resistant mutations in HIV-1 are largely credited to its error prone HIV-1 RT, the time point in the infection cycle that these mutations can arise and if they appear spontaneously without selection pressures both remained enigmatic. Many HIV-1 RT mutational in vitro studies utilized reporter genes (LacZ) a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010370
更新日期:2020-12-31 00:00:00
abstract::d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear. In this study, using the loperamide-induced constipation Kunming mice as the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20225721
更新日期:2019-11-14 00:00:00
abstract::Innate lymphoid cells (ILC) are important players of early immune defenses in situations like lymphoid organogenesis or in case of immune response to inflammation, infection and cancer. Th1 and Th2 antagonism is crucial for the regulation of immune responses, however mechanisms are still unclear for ILC functions. ILC...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215493
更新日期:2019-11-04 00:00:00
abstract::As a major site of glucose uptake following a meal, skeletal muscle has an important role in whole-body glucose metabolism. Evidence in humans and animal models of insulin resistance and type 2 diabetes suggests that alterations in mitochondrial characteristics accompany the development of skeletal muscle insulin resi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21186948
更新日期:2020-09-22 00:00:00
abstract::Metronidazole (MD) is known as a periodontitis medicine and has been widely used in antibiotics for resistance to anaerobic bacteria, periodontal disease, and other threats. To treat diseases, drug delivery carriers are needed with a high bioadhesive property and enhanced drug penetration. Poly (acrylic acid) (PAA) hy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010187
更新日期:2019-12-26 00:00:00
abstract::A series of perylene diimide (PDI) derivatives have been investigated at the CAM-B3LYP/6-31G(d) and the TD-B3LYP/6-31+G(d,p) levels to design solar cell acceptors with high performance in areas such as suitable frontier molecular orbital (FMO) energies to match oligo(thienylenevinylene) derivatives and improved charge...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17050721
更新日期:2016-05-13 00:00:00
abstract::The influenza A virus (IAV), a member of the Orthomyxoviridae family, is a highly transmissible respiratory pathogen and represents a continued threat to global health with considerable economic and social impact. IAV is a zoonotic virus that comprises a plethora of strains with different pathogenic profiles. The diff...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18081673
更新日期:2017-08-01 00:00:00
abstract::With the aim of contributing to the knowledge about their potential therapeutic activity, we determined the biological activities of cyanidin and its selected O-glycosides in relation to erythrocytes (RBCs) and human dermal vascular endothelial cells (HMEC-1). Furthermore, on the basis of changes in the physical/funct...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081928
更新日期:2019-04-19 00:00:00
abstract::It has recently been recognized that inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), upregulate the secretion of matrix metalloproteinase-9 (MMP-9) from cancer cells and thereby promote peritoneal dissemination. In this study, we found that TNF-α also stimulated peritoneal mesothelial cells to secrete...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123961
更新日期:2018-12-09 00:00:00
abstract::The prostate gland contains a high level of intracellular zinc, which is dramatically diminished during prostate cancer (PCa) development. Owing to the unclear role of zinc in this process, therapeutic applications using zinc are limited. This study aimed to clarify the role of zinc and its underlying mechanism in the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103062
更新日期:2018-10-08 00:00:00
abstract:BACKGROUND:In pregnancy, excessive inflammation and break down of immunologic tolerance can contribute to miscarriage. Endothelial cells (ECs) are able to orchestrate the inflammatory processes by secreting pro-inflammatory mediators and bactericidal factors by modulating leakiness and leukocyte trafficking, via the ex...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071604
更新日期:2019-03-31 00:00:00
abstract::Stimulation of thermogenesis in brown adipose tissue (BAT) could have far-reaching health benefits in combatting obesity and obesity-related complications. Apolipoprotein A-IV (ApoA-IV), produced by the gut and the brain in the presence of dietary lipids, is a well-known short-term satiating protein. While our previou...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031221
更新日期:2021-01-27 00:00:00
abstract::A new approach for encoding DNA sequences as input for DNA sequence analysis is proposed using the error correction coding theory of communication engineering. The encoder was designed as a convolutional code model whose generator matrix is designed based on the degeneracy of codons, with a codon treated in the model ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14048393
更新日期:2013-04-16 00:00:00
abstract::The aim of this study was to prepare micronized taxifolin powder using the supercritical antisolvent precipitation process to improve the dissolution rate of taxifolin. Ethanol was used as solvent and carbon dioxide was used as an antisolvent. The effects of process parameters, such as temperature (35-65 °C), pressure...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13078869
更新日期:2012-01-01 00:00:00
abstract::Myostatin is a negative regulator of muscle cell growth and proliferation. Furthermore, myostatin directly affects the expression of 14q32 microRNAs by binding the 14q32 locus. Direct inhibition of 14q32 microRNA miR-495-3p decreased postinterventional restenosis via inhibition of both vascular smooth muscle cell (VSM...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21103508
更新日期:2020-05-15 00:00:00