Abstract：
BACKGROUND:Genetic factors account for about fifty percent of the risk for alcoholism and alcohol dependence (AD) has been reported to be influenced by cannabinoid receptors (CBRs) and the endocannabinoid system (ECS). Previous studies have focused on cannabinoids and alcohol-related effects in the CNS; however, the role CBRs play on alcohol effects in the immune system has not been elucidated yet. Since alcohol can affect immune responses and have detrimental effects on immune cells such as dendritic cells (DCs), we hypothesize that alcohol can exert its effects on DCs by modulating changes in CBRs, which in turn can regulate important DC functions such as cytokine production. METHODS:Therefore, we studied the expression of CNR1 and CNR2, and the novel cannabinoid G protein-coupled receptor (GPCR) 55 (GPR55) in human monocyte-derived dendritic cells (MDDCs) from alcohol users. CNR1, CNR2, and GPR55 genes were measured by qRT-PCR and protein by flow cytometry. MDDCs from alcohol users show significantly higher levels of CNR2 and GPR55 compared to MDDCs from non-users. These findings were further confirmed using MDDCs treated with alcohol. Inflammatory cytokines were measured in EtOH-treated and non-treated cells by antibody array. RESULTS:Functional effects of CBRs on MDDCs were shown by CB2 and GPR55 siRNA transfection. Transfected EtOH-treated cells showed significantly higher levels of proinflammatory cytokine production as measured by IL-1β expression. Our results provide insights into alcohol mechanisms of DC regulation and show, for the first time, that alcohol is inducing CNR2 and GPR55 in human DCs.
journal_name
Drug Alcohol Dependjournal_title
Drug and alcohol dependenceauthors
Agudelo M,Yndart A,Morrison M,Figueroa G,Muñoz K,Samikkannu T,Nair MPdoi
10.1016/j.drugalcdep.2013.08.023subject
Has Abstractpub_date
2013-12-01 00:00:00pages
789-93issue
2eissn
0376-8716issn
1879-0046pii
S0376-8716(13)00339-6journal_volume
133pub_type
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pub_type： 杂志文章,随机对照试验
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journal_title：Drug and alcohol dependence
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更新日期：2020-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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更新日期：2020-05-25 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2001-10-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
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pub_type： 临床试验,杂志文章,随机对照试验
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