Apical targeting of the formin Diaphanous in Drosophila tubular epithelia.

abstract：:The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular ...

journal_title：eLife

authors： Zhang X,Kampourakis T,Yan Z,Sevrieva I,Irving M,Sun YB

更新日期：2017-02-23 00:00:00

abstract：:A study of 3,500 children in the UK shows that data on socioeconomic background and previous educational achievements can better predict how students will perform at school than genetic data. ...

journal_title：eLife

authors： Janssens C

更新日期：2020-03-13 00:00:00

abstract：:The representation of position in the mammalian brain is distributed across multiple neural populations. Grid cell modules in the medial entorhinal cortex (MEC) express activity patterns that span a low-dimensional manifold which remains stable across different environments. In contrast, the activity patterns of hippo...

journal_title：eLife

authors： Agmon H,Burak Y

更新日期：2020-08-11 00:00:00

abstract：:Organisms possessing genetic codes with unassigned codons raise the question of how cellular machinery resolves such codons and how this could impact horizontal gene transfer. Here, we use a genomically recoded Escherichia coli to examine how organisms address translation at unassigned UAG codons, which obstruct propa...

journal_title：eLife

authors： Ma NJ,Hemez CF,Barber KW,Rinehart J,Isaacs FJ

更新日期：2018-10-30 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...

journal_title：eLife

authors： Ye W,Han TW,He M,Jan YN,Jan LY

更新日期：2019-07-18 00:00:00

abstract：:Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and rea...

journal_title：eLife

authors： Rodrigo Albors A,Tazaki A,Rost F,Nowoshilow S,Chara O,Tanaka EM

更新日期：2015-11-14 00:00:00

abstract：:WNK1, a kinase that controls kidney salt homeostasis, also regulates adhesion and migration in CD4+ T cells. Wnk1 is highly expressed in thymocytes, and since migration is important for thymocyte maturation, we investigated a role for WNK1 in mouse thymocyte development. We find that WNK1 is required for the transitio...

journal_title：eLife

authors： Köchl R,Vanes L,Llorian Sopena M,Chakravarty P,Hartweger H,Fountain K,White A,Cowan J,Anderson G,Tybulewicz VL

更新日期：2020-10-14 00:00:00

abstract：:The Deleted in Colorectal Carcinoma (Dcc) receptor plays a critical role in optic nerve development. Whilst Dcc is expressed postnatally in the eye, its function remains unknown as Dcc knockouts die at birth. To circumvent this drawback, we generated an eye-specific Dcc mutant. To study the organization of the retina ...

journal_title：eLife

authors： Vigouroux RJ,Cesar Q,Chédotal A,Nguyen-Ba-Charvet KT

更新日期：2020-02-25 00:00:00

abstract：:When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule dynamics contributes to this proc...

journal_title：eLife

authors： Hooikaas PJ,Damstra HG,Gros OJ,van Riel WE,Martin M,Smits YT,van Loosdregt J,Kapitein LC,Berger F,Akhmanova A

更新日期：2020-12-21 00:00:00

abstract：:RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...

journal_title：eLife

authors： Kroschwald S,Maharana S,Mateju D,Malinovska L,Nüske E,Poser I,Richter D,Alberti S

更新日期：2015-08-04 00:00:00

abstract：:In many plant species, a subset of transcribed genes are characterized by strictly CG-context DNA methylation, referred to as gene body methylation (gbM). The mechanisms that establish gbM are unclear, yet flowering plant species naturally without gbM lack the DNA methyltransferase, CMT3, which maintains CHG (H = A, C...

journal_title：eLife

authors： Wendte JM,Zhang Y,Ji L,Shi X,Hazarika RR,Shahryary Y,Johannes F,Schmitz RJ

更新日期：2019-07-29 00:00:00

abstract：:The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...

journal_title：eLife

authors： Evans B,Griner E,Reproducibility Project: Cancer Biology.

更新日期：2015-07-31 00:00:00

abstract：:Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the ...

journal_title：eLife

authors： Mehta S,Aye-Han NN,Ganesan A,Oldach L,Gorshkov K,Zhang J

更新日期：2014-07-23 00:00:00

abstract：:The sense of ownership of one's body is important for survival, e.g., in defending the body against a threat. However, in addition to affecting behavior, it also affects perception of the world. In the case of visuospatial perception, it has been shown that the sense of ownership causes external space to be perceptual...

journal_title：eLife

authors： van der Hoort B,Reingardt M,Ehrsson HH

更新日期：2017-08-17 00:00:00

abstract：:Gustatory pheromones play an essential role in shaping the behavior of many organisms. However, little is known about the processing of taste pheromones in higher order brain centers. Here, we describe a male-specific gustatory circuit in Drosophila that underlies the detection of the anti-aphrodisiac pheromone (3R,11...

journal_title：eLife

authors： Shankar S,Chua JY,Tan KJ,Calvert ME,Weng R,Ng WC,Mori K,Yew JY

更新日期：2015-06-17 00:00:00

abstract：:In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational ch...

journal_title：eLife

authors： Cheng TC,Hong C,Akey IV,Yuan S,Akey CW

更新日期：2016-10-04 00:00:00

abstract：:Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...

journal_title：eLife

authors： Grosso AR,Leite AP,Carvalho S,Matos MR,Martins FB,Vítor AC,Desterro JM,Carmo-Fonseca M,de Almeida SF

更新日期：2015-11-17 00:00:00

abstract：:Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission y...

journal_title：eLife

authors： Baker K,Gyamfi IA,Mashanov GI,Molloy JE,Geeves MA,Mulvihill DP

更新日期：2019-09-30 00:00:00

abstract：:In the fruit fly optic lobe, T4 and T5 cells represent the first direction-selective neurons, with T4 cells responding selectively to moving brightness increments (ON) and T5 cells to brightness decrements (OFF). Both T4 and T5 cells comprise four subtypes with directional tuning to one of the four cardinal directions...

journal_title：eLife

authors： Haag J,Mishra A,Borst A

更新日期：2017-08-22 00:00:00

abstract：:Transient receptor potential vanilloid 5 (TRPV5) is a highly calcium selective ion channel that acts as the rate-limiting step of calcium reabsorption in the kidney. The lack of potent, specific modulators of TRPV5 has limited the ability to probe the contribution of TRPV5 in disease phenotypes such as hypercalcemia a...

journal_title：eLife

authors： Hughes TE,Del Rosario JS,Kapoor A,Yazici AT,Yudin Y,Fluck EC 3rd,Filizola M,Rohacs T,Moiseenkova-Bell VY

更新日期：2019-10-25 00:00:00

abstract：:Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron...

journal_title：eLife

authors： Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

更新日期：2019-05-14 00:00:00

abstract：:Processing bodies (PBs) are cytoplasmic mRNP granules that assemble via liquid-liquid phase separation and are implicated in the decay or storage of mRNAs. How PB assembly is regulated in cells remains unclear. Previously, we identified the ATPase activity of the DEAD-box protein Dhh1 as a key regulator of PB dynamics...

journal_title：eLife

authors： Sachdev R,Hondele M,Linsenmeier M,Vallotton P,Mugler CF,Arosio P,Weis K

更新日期：2019-01-16 00:00:00

abstract：:Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites ...

journal_title：eLife

authors： Horne JA,Langille C,McLin S,Wiederman M,Lu Z,Xu CS,Plaza SM,Scheffer LK,Hess HF,Meinertzhagen IA

更新日期：2018-11-01 00:00:00

abstract：:As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Blum et al., 2015), that described how we intended to replicate selected experiments from the paper 'Transcriptional amplification in tumor cells with elevated c-Myc' (Lin et al., 2012). Here we report the results. We found overe...

journal_title：eLife

authors： Lewis LM,Edwards MC,Meyers ZR,Talbot CC Jr,Hao H,Blum D,Reproducibility Project: Cancer Biology.

更新日期：2018-01-09 00:00:00

abstract：:In bacteria, multicellular behaviors are regulated by cell-cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imagin...

journal_title：eLife

authors： Ducret A,Fleuchot B,Bergam P,Mignot T

更新日期：2013-07-23 00:00:00

abstract：:The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...

journal_title：eLife

authors： Isaac RS,Jiang F,Doudna JA,Lim WA,Narlikar GJ,Almeida R

更新日期：2016-04-28 00:00:00

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...

journal_title：eLife

authors： Ouyang M,Peng Q,Jeon T,Heyne R,Chalak L,Huang H

更新日期：2020-12-22 00:00:00

abstract：:The loss of previously adaptive traits is typically linked to relaxation in selection, yet the molecular steps leading to such repeated losses are rarely known. Molecular studies of loss have tended to focus on gene sequences alone, but overlooking other aspects of protein expression might underestimate phenotypic div...

journal_title：eLife

authors： Sadier A,Davies KT,Yohe LR,Yun K,Donat P,Hedrick BP,Dumont ER,Dávalos LM,Rossiter SJ,Sears KE

更新日期：2018-12-18 00:00:00