当前位置： SCI文献检索 > BIOMATERIALS期刊下所有文献 > The role of RhoA kinase inhibition in human placenta-derived multipotent cells on neural phenotype and cell survival.

The role of RhoA kinase inhibition in human placenta-derived multipotent cells on neural phenotype and cell survival.

Abstract：

:Current advances in stem cell biology have brought much hope for therapy of neuro-degenerative diseases. However, neural stem cells (NSCs) are rare adult stem cells, and the use of non-NSCs requires efficient and high-yielding lineage-specific differentiation prior to transplantation for efficacy. We report on the efficient differentiation of placental-derived multipotent cells (PDMCs) into a neural phenotype with use of Y-27632, a clinically compliant small molecular inhibitor of Rho kinase (ROCK) which is a major mediator of cytoskeleton dynamics. Y-27632 does not induce differentiation of PDMC toward the mesodermal lineages of adipogenesis and osteogenesis, but rather a neural-like morphology, with rapid development of cell extensions and processes within 24 h. Compared with conventional neurogenic differentiation agents, Y-27632 induces a higher percentage of neural-like cells in PDMCs without arresting proliferation or cell cycle dynamics. Y-27632-treated PDMCs express several neural lineage genes at the RNA and protein level, including nestin, MAP2, and GFAP. The effect of the ROCK inhibitor is cell-specific to PDMCs, and is mainly mediated through the ROCK2 isoform and its downstream target, myosin II. Our data suggest that ROCK inhibition and cytoskeletal rearrangement may allow for induction of a neural phenotype in PDMCs without compromising cell survival.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Wang CH,Wu CC,Hsu SH,Liou JY,Li YW,Wu KK,Lai YK,Yen BL

doi

10.1016/j.biomaterials.2012.12.034

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

3223-30

issue

13

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(12)01414-7

journal_volume

34

pub_type

杂志文章

相关文献

BIOMATERIALS文献大全
  • Protein and bacterial fouling characteristics of peptide and antibody decorated surfaces of PEG-poly(acrylic acid) co-polymers.

    abstract：:The potential for base poly(ethylene glycol) graft poly(acrylic acid) PEG-g-PA copolymers and surface-modified PEG-g-PA materials to inhibit random protein fouling and bacterial adhesion are investigated. PEG-g-PA co-polymers were synthesized that inhibited non-specific protein and cellular adhesion. PEG-g-PA co-polym...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2003.09.020

    authors： Wagner VE,Koberstein JT,Bryers JD

    更新日期：2004-05-01 00:00:00

  • Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells.

    abstract：:The recreation of an in vitro microenvironment to understand and manipulate the proliferation and migration of invasive breast cancer cells may allow one to put a halt to their metastasis capacity. Invasive cancer cells have been linked to embryonic stem (ES) cells as they possess certain similar characteristics and g...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2011.02.035

    authors： Raof NA,Raja WK,Castracane J,Xie Y

    更新日期：2011-06-01 00:00:00

  • Calcium phosphate coating of nickel-titanium shape-memory alloys. Coating procedure and adherence of leukocytes and platelets.

    abstract：:Nickel-titanium shape-memory alloys (NiTi-SMA) were coated with calcium phosphate by dipping in oversaturated calcium phosphate solution. The layer thickness (typically 5-20 micrometer) can be varied by choice of the immersion time. The porous nature of the layer of microcrystals makes it mechanically stable enough to...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(03)00241-2

    authors： Choi J,Bogdanski D,Köller M,Esenwein SA,Müller D,Muhr G,Epple M

    更新日期：2003-09-01 00:00:00

  • The use of a gold nanoparticle-based adjuvant to improve the therapeutic efficacy of hNgR-Fc protein immunization in spinal cord-injured rats.

    abstract：:As a common receptor for three myelin associated inhibitors, Nogo-66 receptor (NgR) mediates their inhibitory activities on neurite outgrowth in the adult mammalian central nervous system (CNS). Therapeutic vaccination protocol targeting NgR emulsified with Freund's adjuvant (FA) has been used in spinal cord injury (S...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2011.07.009

    authors： Wang YT,Lu XM,Zhu F,Huang P,Yu Y,Zeng L,Long ZY,Wu YM

    更新日期：2011-11-01 00:00:00

  • Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering.

    abstract：:Injectable, biodegradable scaffolds are important biomaterials for tissue engineering and drug delivery. Hydrogels derived from natural polysaccharides are ideal scaffolds as they resemble the extracellular matrices of tissues comprised of various glycosaminoglycans (GAGs). Here, we report a new class of biocompatible...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2008.12.080

    authors： Tan H,Chu CR,Payne KA,Marra KG

    更新日期：2009-05-01 00:00:00

  • Influence of titanium surfaces on attachment of osteoblast-like cells in vitro.

    abstract：:Implant surface topography influences osteoblastic proliferation, differentiation and extracellular matrix protein expressions. Studies on preliminary interactions of osteoblast-like cells on implant interface through in vitro systems, can give lucid insights to osseo-integrative efficacies of when in vivo implants. I...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(03)00571-4

    authors： Jayaraman M,Meyer U,Bühner M,Joos U,Wiesmann HP

    更新日期：2004-02-01 00:00:00

  • The influence of BMP-2 and its mode of delivery on the osteoconductivity of implant surfaces during the early phase of osseointegration.

    abstract：:Osteogenic agents, such as bone morphogenetic protein-2 (BMP-2), can stimulate the degradation as well as the formation of bone. Hence, they could impair the osteoconductivity of functionalized implant surfaces. We assessed the effects of BMP-2 and its mode of delivery on the osteoconductivity of dental implants with ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2007.02.003

    authors： Liu Y,Enggist L,Kuffer AF,Buser D,Hunziker EB

    更新日期：2007-06-01 00:00:00

  • Encapsulated cells: an atomic force microscopy study.

    abstract：:Two types of cells--human platelets and spore cells--were encapsulated in polymer shells by adsorbing polyanions and polycations in a stepwise fashion. The encapsulated cells were attached to gold and silicon surfaces and their morphological and adhesion properties were studied in air using tapping mode atomic force m...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2003.10.061

    authors： Yu M,Ivanisevic A

    更新日期：2004-08-01 00:00:00

  • Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy.

    abstract：:Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of the relationships between the scaffold properties and its degradation kinetics, as well as the cell interactions and the promotion of new matrix deposition. Here we present the use of non...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2007.12.049

    authors： Rice WL,Firdous S,Gupta S,Hunter M,Foo CW,Wang Y,Kim HJ,Kaplan DL,Georgakoudi I

    更新日期：2008-05-01 00:00:00

  • Low-aspect ratio nanopatterns on bioinert alumina influence the response and morphology of osteoblast-like cells.

    abstract：:Topographical features on the nanometer scale are known to influence cellular behavior. The response of specific cell types to various types of surface structures is currently still being investigated. Alumina ceramics play an important role as biomaterials, e.g., in medical and dental applications. In this study, we ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2015.05.026

    authors： Wittenbrink I,Hausmann A,Schickle K,Lauria I,Davtalab R,Foss M,Keller A,Fischer H

    更新日期：2015-09-01 00:00:00

  • Adhesion and spreading of cultured endothelial cells on modified and unmodified poly (ethylene terephthalate): a morphological study.

    abstract：:The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture poly(ethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than thos...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(89)90058-6

    authors： van Wachem PB,Schakenraad JM,Feijen J,Beugeling T,van Aken WG,Blaauw EH,Nieuwenhuis P,Molenaar I

    更新日期：1989-10-01 00:00:00

  • Controlling the kinetics of thiol-maleimide Michael-type addition gelation kinetics for the generation of homogenous poly(ethylene glycol) hydrogels.

    abstract：:The development of synthetic hydrogels analogs for the extracellular matrix has proven a useful and important tool to study the role of specific signals on biological outcomes in vitro and to serve as scaffolds for tissue repair. Although the importance of physical properties (e.g. microstructure and stiffness) in the...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2016.05.053

    authors： Darling NJ,Hung YS,Sharma S,Segura T

    更新日期：2016-09-01 00:00:00

  • A recombinant slow-release PACAP-derived peptide alleviates diabetes by promoting both insulin secretion and actions.

    abstract：:Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuroendocrine factor that activates both the receptor VPAC1 and VPAC2. Although PACAP possesses insulinotropic activity, its therapeutic application is limited by the extremely short acting half-life and the stimulatory effects on glucagon production via...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2015.01.064

    authors： Ma Y,Fang S,Zhao S,Wang X,Wang D,Ma M,Luo T,Hong A

    更新日期：2015-05-01 00:00:00

  • Assembly of upconversion nanophotosensitizer in vivo to achieve scatheless real-time imaging and selective photodynamic therapy.

    abstract：:A perfect "off" to "on" switch of the therapeutic function is very important to minimize the phototoxicity of nanoplatforms assisted imaging-guided photodynamic therapy (PDT) of cancer. Current approaches rely on preloaded photosensitizers, where the off/on state of PDT is regulated by the sensitizing light of photose...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.02.015

    authors： Feng Y,Wu Y,Zuo J,Tu L,Que I,Chang Y,Cruz LJ,Chan A,Zhang H

    更新日期：2019-05-01 00:00:00

  • Degradation of polydispersed poly(L-lactic acid) to modulate lactic acid release.

    abstract：:Polydispersed poly(L-lactic acid) (PLLA) membranes comprised of blends of monodispersed PLLA of weight average molecular weight of 82,500 and 7600 were fabricated to investigate the effect of polydispersity on degradation characteristics. The PLLA blends exhibited large spherulites of high molecular weight chains embe...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(95)98816-w

    authors： von Recum HA,Cleek RL,Eskin SG,Mikos AG

    更新日期：1995-04-01 00:00:00

  • Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds.

    abstract：:Angiogenesis is critical in the early stage of reparative processes and tissue regeneration, but the persistence of a vascular network may interfere with later transformation/maturation in naturally avascular tissues such as articular cartilage. Our supposition is that the timed delivery of an anti-angiogenic factor i...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2008.10.020

    authors： Sun XD,Jeng L,Bolliet C,Olsen BR,Spector M

    更新日期：2009-02-01 00:00:00

  • Prion inhibition with multivalent PrPSc binding compounds.

    abstract：:Quinacrine and related heterocyclic compounds have antiprion activity. Since the infectious pathogen of prion diseases is composed of multimeric PrP(Sc) assemblies, we hypothesized that this antiprion property could be enhanced by attaching multiple quinacrine-derived chloroquinoline or acridine moieties to a scaffold...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2012.06.004

    authors： Mays CE,Joy S,Li L,Yu L,Genovesi S,West FG,Westaway D

    更新日期：2012-10-01 00:00:00

  • Toxicological characteristics of nanoparticulate anatase titanium dioxide in mice.

    abstract：:In an effort to examine liver injury, immune response, and other physiological effects in mice caused by intragastric administration of nanoparticulate anatase titanium dioxide (5nm), we assessed T lymphocytes, B lymphocyte and NK lymphocyte counts, hematological indices, biochemical parameters of liver functions, and...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2009.10.003

    authors： Duan Y,Liu J,Ma L,Li N,Liu H,Wang J,Zheng L,Liu C,Wang X,Zhao X,Yan J,Wang S,Wang H,Zhang X,Hong F

    更新日期：2010-02-01 00:00:00

  • Enhanced expression of the osteoblastic phenotype on substrates that modulate fibronectin conformation and integrin receptor binding.

    abstract：:Integrins represent the primary mechanism of cell-extracellular matrix interactions and control cell morphology, proliferation, and differentiation. We have previously shown that substrate-dependent modulation of adsorbed fibronectin (Fn) conformation alters alpha5beta1 integrin binding to Fn and directs C2C12 myoblas...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(01)00387-8

    authors： Stephansson SN,Byers BA,García AJ

    更新日期：2002-06-01 00:00:00

  • Debye function analysis and 2D imaging of nanoscaled engineered bone.

    abstract：:The Debye Function Analysis of diffraction patterns from nanosized mineral crystals showing different average degrees of maturity was carried out on engineered bone samples. The analysis relied on a bivariate family of atomistic hydroxyapatite nanocrystal models and provided information about crystal structure, size a...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.07.051

    authors： Guagliardi A,Cedola A,Giannini C,Ladisa M,Cervellino A,Sorrentino A,Lagomarsino S,Cancedda R,Mastrogiacomo M

    更新日期：2010-11-01 00:00:00

  • Reversible opening of the blood-brain barrier by claudin-5-binding variants of Clostridium perfringens enterotoxin's claudin-binding domain.

    abstract：:The blood-brain barrier (BBB) prevents entry of neurotoxic substances but also that of drugs into the brain. Here, the paracellular barrier is formed by tight junctions (TJs) with claudin-5 (Cldn5) being the main sealing constituent. Transient BBB opening by targeting Cldn5 could improve paracellular drug delivery. Th...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2018.01.028

    authors： Neuhaus W,Piontek A,Protze J,Eichner M,Mahringer A,Subileau EA,Lee IM,Schulzke JD,Krause G,Piontek J

    更新日期：2018-04-01 00:00:00

  • Riboflavin carrier protein-targeted fluorescent USPIO for the assessment of vascular metabolism in tumors.

    abstract：:Riboflavin (Rf) and its metabolic analogs flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are essential for normal cellular growth and function. Their intracellular transport is regulated by the riboflavin carrier protein (RCP), which has been shown to be over-expressed by metabolically active cancer...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2012.08.036

    authors： Jayapaul J,Arns S,Lederle W,Lammers T,Comba P,Gätjens J,Kiessling F

    更新日期：2012-12-01 00:00:00

  • Combinatorial co-encapsulation of hydrophobic molecules in poly(lactide-co-glycolide) microparticles.

    abstract：:There is great interest for developing poly(lactide-co-glycolide) (PLGA) based particles for targeted delivery and controlled release of encapsulated biological molecules. These PLGA particles can be used to deliver proteins, small molecule drugs and nucleotides. Furthermore, it has been shown that the co-encapsulatio...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.01.032

    authors： Acharya AP,Lewis JS,Keselowsky BG

    更新日期：2013-04-01 00:00:00

  • The promotion of bone healing by progranulin, a downstream molecule of BMP-2, through interacting with TNF/TNFR signaling.

    abstract：:Endochondral ossification plays a key role in the bone healing process, which requires normal cartilage callus formation. Progranulin (PGRN) growth factor is known to enhance chondrocyte differentiation and endochondral ossification during development, yet whether PGRN also plays a role in bone regeneration remains un...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.05.030

    authors： Zhao YP,Tian QY,Frenkel S,Liu CJ

    更新日期：2013-09-01 00:00:00

  • The molecular mechanism of mediation of adsorbed serum proteins to endothelial cells adhesion and growth on biomaterials.

    abstract：:To explore molecular mechanism of mediation of adsorbed proteins to cell adhesion and growth on biomaterials, this study examined endothelial cell adhesion, morphology and viability on bare and titanium nitride (TiN) coated nickel titanium (NiTi) alloys and chitosan film firstly, and then identified the type and amoun...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.04.028

    authors： Yang D,Lü X,Hong Y,Xi T,Zhang D

    更新日期：2013-07-01 00:00:00

  • Selective antileukemia effect of stabilized nanohybrid vesicles based on cholesteryl succinyl silane.

    abstract：:A nanohybrid vesicle was developed from cholesteryl succinyl silane(CSS) in combination of sol-gel process and self-assembly technique. The silicalike surface adds CSS vesicles remarkably high stability against destabilization in blood or leakage of drug cargos. It was found that CSS vesicles alone exhibited selective...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2011.08.043

    authors： Ma Y,Dai Z,Zha Z,Gao Y,Yue X

    更新日期：2011-12-01 00:00:00

  • Multiscale requirements for bioencapsulation in medicine and biotechnology.

    abstract：:Bioencapsulation involves the envelopment of tissues or biological active substances in semipermeable membranes. Bioencapsulation has been shown to be efficacious in mimicking the cell's natural environment and thereby improves the efficiency of production of different metabolites and therapeutic agents. The field of ...

    journal_title：Biomaterials

    pub_type： 杂志文章,评审

    doi：10.1016/j.biomaterials.2009.01.014

    authors： de Vos P,Bucko M,Gemeiner P,Navrátil M,Svitel J,Faas M,Strand BL,Skjak-Braek G,Morch YA,Vikartovská A,Lacík I,Kolláriková G,Orive G,Poncelet D,Pedraz JL,Ansorge-Schumacher MB

    更新日期：2009-05-01 00:00:00

  • Human embryonic stem cell-derived cardiovascular progenitor cells efficiently colonize in bFGF-tethered natural matrix to construct contracting humanized rat hearts.

    abstract：:Bioengineering of whole hearts using human embryonic stem cells (hESCs)-derived cardiovascular progenitor cells (CPCs) and natural matrices is a promising approach to overcome organ donor shortage threatening millions of patients awaiting for heart transplantation. Here, we developed a novel strategy for generation of...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2017.10.054

    authors： Rajabi S,Pahlavan S,Ashtiani MK,Ansari H,Abbasalizadeh S,Sayahpour FA,Varzideh F,Kostin S,Aghdami N,Braun T,Baharvand H

    更新日期：2018-02-01 00:00:00

  • Creep in a palladium-enriched high-copper amalgam.

    abstract：:Palladium additions to a dispersed phase high-copper amalgam have been shown recently to suppress markedly the eta' (Cu6Sn5) concentration and decrease creep. A detailed study of the dental and 24 h creep for Pd containing high-copper amalgams and six commercial controls as a function of applied temperature and stress...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(88)90086-5

    authors： Greener EH,Chung KH,Lin JH

    更新日期：1988-05-01 00:00:00

  • Manufacture of porous polymer nerve conduits by a novel low-pressure injection molding process.

    abstract：:A method to fabricate porous, biodegradable conduits using a combined injection molding, thermally induced phase transition technique was developed which produced conduits with dimensionally toleranced, longitudinally aligned channels. The geometry of the channels was designed to approximate the architecture of periph...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(02)00409-x

    authors： Sundback C,Hadlock T,Cheney M,Vacanti J

    更新日期：2003-02-01 00:00:00