Abstract:
:Bioengineering of whole hearts using human embryonic stem cells (hESCs)-derived cardiovascular progenitor cells (CPCs) and natural matrices is a promising approach to overcome organ donor shortage threatening millions of patients awaiting for heart transplantation. Here, we developed a novel strategy for generation of heart constructs by repopulating engineered decellularized rat hearts using hESCs-derived CPCs. Careful expansion of CPCs in a scalable stirred-suspension bioreactor combined with step-wise seeding (60 million cells in 3 steps of 20 million per 1.5 h) onto decellularized hearts containing immobilized basic fibroblast growth factor (bFGF) resulted in improved retention of CPCs and differentiation to cardiomyocytes, smooth muscle cells and endothelial cells as evaluated by immunohistochemistry and qRT-PCR. We observed spontaneous and synchronous contractions of humanized hearts after 12 days of perfusion as well as advanced alignment of myofilaments. Our study provides a robust platform for generation of artificial human hearts and resolves major bottlenecks hindering further development of this technology.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Rajabi S,Pahlavan S,Ashtiani MK,Ansari H,Abbasalizadeh S,Sayahpour FA,Varzideh F,Kostin S,Aghdami N,Braun T,Baharvand Hdoi
10.1016/j.biomaterials.2017.10.054subject
Has Abstractpub_date
2018-02-01 00:00:00pages
99-112eissn
0142-9612issn
1878-5905pii
S0142-9612(17)30720-2journal_volume
154pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::Decellularized adipose tissue (DAT) has shown promise as an adipogenic bioscaffold for soft tissue augmentation and reconstruction. The objective of the current study was to investigate the effects of allogeneic adipose-derived stem/stromal cells (ASCs) on in vivo fat regeneration in DAT bioscaffolds using an immunoco...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.08.053
更新日期:2015-12-01 00:00:00
abstract::This paper describes a method of foaming a polymer system comprising poly(ethyl methacrylate)/tetrahydrofurfuryl methacrylate (PEMA/THFMA), characterisation of the resulting porosity and use of the foam for chondrocyte culture. The potential for this polymer system to support cartilage repair has been investigated pre...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.10.023
更新日期:2004-08-01 00:00:00
abstract::A type III glass-ionomer cement (Vidrion F), currently used as fast-setting lining material and fissure sealant, was implanted into rat dental alveolus immediately after tooth extraction and its biocompatibility was analysed in terms of incorporation into alveolar bone in the wound healing process. Histological and hi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(96)00110-x
更新日期:1997-01-01 00:00:00
abstract::Endochondral ossification plays a key role in the bone healing process, which requires normal cartilage callus formation. Progranulin (PGRN) growth factor is known to enhance chondrocyte differentiation and endochondral ossification during development, yet whether PGRN also plays a role in bone regeneration remains un...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.05.030
更新日期:2013-09-01 00:00:00
abstract::Tissue engineering of the cornea could overcome shortages of donor corneas for transplantation and improve quality. Our aim was to grow an endothelial layer on a substratum suitable for transplant. Silkworm (Bombyx mori) fibroin was prepared as 5 μm thick transparent membranes. The B4G12 cell line was used to assess a...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.12.034
更新日期:2011-06-01 00:00:00
abstract::The authors have evaluated adhesive protein expression and cytokine production by human umbilical vein endothelial cells cultured in contact with polyethylene terephthalate (PET). ELAM-1, ICAM-1 and VCAM-1 expression was determined by flow cytometry; the concentration of interleukin-1 alpha (IL-1 alpha), interleukin-6...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(96)00037-3
更新日期:1996-11-01 00:00:00
abstract::In the present study, the effects of a calcium magnesium silicate bioactive ceramic (akermanite) on proliferation and osteoblastic differentiation of human bone marrow stromal cells (hBMSC) have been investigated and compared with the classical ceramic (beta-tricalcium phosphate, beta-TCP). Akermanite and beta-TCP dis...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.07.027
更新日期:2006-11-01 00:00:00
abstract::Highly crosslinked UHMWPEs have demonstrated improved in vitro wear properties; however, there is concern regarding loss of fracture resistance and ductility. The goals of this study were to evaluate the micromechanisms of failure under uniaxial tension and to determine the effect of gamma radiation-induced crosslinki...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00277-1
更新日期:2003-10-01 00:00:00
abstract::Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P2O5. We investigated the effect of varying the...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.09.094
更新日期:2004-06-01 00:00:00
abstract::Thermosensitive polymers poly(N-isopropylacrylamide) (PNIPAM), poly(N-vinylcaprolactam) (PVCL) and PVCL grafted with amphiphilic poly(ethylene oxide) (PEO) chains (PVCL-graft-C11EO42) were prepared and characterized and their putative cytotoxicity was evaluated. The cytotoxicity of these thermosensitive polymers and t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.09.008
更新日期:2005-06-01 00:00:00
abstract::In vitro solubility tests of hydroxyapatite, tetracalcium phosphate or tricalcium phosphate particles were performed in lactate, citrate, Gomoris or Michaelis buffer with pH 6.2 or 7.2 and in aqua destillata. The results showed that in general the solubility decreased in the order tetracalcium phosphate greater than t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(90)90067-z
更新日期:1990-09-01 00:00:00
abstract::Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for h...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.04.036
更新日期:2015-08-01 00:00:00
abstract::Bioengineered scaffolds have the potential to support and guide injured axons after spinal cord injury, contributing to neural repair. In previous studies we have reported that templated agarose scaffolds can be fabricated into precise linear arrays and implanted into the partially injured spinal cord, organizing grow...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.10.070
更新日期:2013-02-01 00:00:00
abstract::In the present study, we evaluated, in mice, the efficacy of the tetrafunctional block copolymer 704 as a nonviral gene delivery vector to the lungs. SPECT/CT molecular imaging of gene expression, biochemical assays, and immunohistochemistry were used. Our dataset shows that the formulation 704 resulted in higher leve...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.12.051
更新日期:2015-03-01 00:00:00
abstract::Three proprietary dental resins were found to include acrylates (as distinct from methacrylates). As acrylates are well known to have adverse skin reactions, the nature and content of the acrylates were obtained. One resin contained methyl acrylate, and the other two ethyl acrylate. Also, the extractable free monomer ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90047-e
更新日期:1991-08-01 00:00:00
abstract::Membranes, clinically used to improve bone regeneration according to the osteopromotion principle, have primarily been made of expanded polytetrafluoroethylene (Gore-Tex Membrane). Recently, different types of biodegradable membranes have become available. This investigation explored the osteopromotive potential of 10...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(95)93857-a
更新日期:1995-05-01 00:00:00
abstract::Bone tissue is an anisotropic non-homogeneous composite material composed of inorganic, bone mineral fibres (hydroxyapatite) embedded in an organic matrix (type I collagen and non-collagenous proteins). Factors contributing to the overall mechanical behaviour include constituent volume fraction, mechanical properties,...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(94)90263-1
更新日期:1994-01-01 00:00:00
abstract::Matrix systems used to study complex three-dimensional (3D) cellular processes like mammary epithelial tissue morphogenesis and tumorigenesis ex vivo often require ill-defined biological components, which lead to poor reproducibility and a lack of control over physical parameters. In this study, a well-defined, tunabl...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.10.007
更新日期:2017-01-01 00:00:00
abstract::Brain tissue inflammatory responses, including neuronal loss and gliosis at the neural electrode/tissue interface, limit the recording stability and longevity of neural probes. The neural adhesion molecule L1 specifically promotes neurite outgrowth and neuronal survival. In this study, we covalently immobilized L1 on ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.09.033
更新日期:2011-01-01 00:00:00
abstract::While subcutaneous tissue has been proposed as a potential site for pancreatic islet transplantation, concern remains that the microvasculature of subcutaneous tissue is too poor to support transplanted islets. In an effort to overcome this limitation, we evaluated whether fibrin gel with human adipose-derived stem ce...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.06.018
更新日期:2013-10-01 00:00:00
abstract::The non-collagenous proteins of mature cortical rabbit bone and 3 wk old bone specimens formed in titanium implant bone harvest chambers, were separated by means of fine pressure liquid chromatography. It was found that a sufficient amount of bone could be obtained from five bone harvest chambers in five rabbits durin...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(90)90158-m
更新日期:1990-04-01 00:00:00
abstract::Alzheimer disease (AD) is a neurodegenerative disorder and the most common form of dementia. Histopathologically is characterized by the presence extracellular neuritic plaques and with a large number of neurons lost. In this paper, we design a new nanomaterial, graphene quantum dots (GQDs) conjugated neuroprotective ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.08.021
更新日期:2016-11-01 00:00:00
abstract::A quantitative comparison between nuclear DNA release from carriers and their transfection activity would be highly useful for improving the effectiveness of non-viral gene vectors. We previously reported that, for condensed DNA particles, a close relationship exists between the efficiency of DNA release and transfect...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.01.049
更新日期:2012-05-01 00:00:00
abstract::The clinical application of cancer radiotherapy is critically impeded by hypoxia-induced radioresistance, insufficient DNA damage, and multiple DNA repair mechanisms. Herein we demonstrate a dual-hyperthermia strategy to potentiate radiotherapy by relieving tumor hypoxia and preventing irradiation-induced DNA damage r...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120235
更新日期:2020-10-01 00:00:00
abstract::An ideal approach for bone tissue engineering allows for osteoconductivity, osteoinductivity, and cell transplantation. In this study, we examined coprecipitation and surface adsorption schemes with respect to their abilities to control the spatial quantity and localization of a model protein, bovine serum albumin (BS...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.07.043
更新日期:2006-03-01 00:00:00
abstract::The aim of the present study was to evaluate the potential of acellular dermal matrix as a carrier for delivery of stem cells to the site of soft tissue defect in a murine skin injury model and to determine the potential of stem cells delivered via such an approach to successfully engraft, survive and differentiate lo...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.11.026
更新日期:2008-04-01 00:00:00
abstract::A new implant surface has been developed with the purpose of avoiding as much stress shielding as possible, and thus prolong the prosthesis lifespan. The aim of this study was to investigate the in vitro effect of this new ultra-high roughness and dense Titanium (Ti) surface (PG60, Ra = 74 microm) in comparison with m...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.01.010
更新日期:2005-08-01 00:00:00
abstract::Dexamethasone, a steroidal anti-inflammatory drug, was incorporated into porous biodegradable polymer scaffolds for sustained release. The slowly released dexamethasone from the degrading scaffolds was hypothesized to locally modulate the proliferation and differentiation of various cells. Dexamethasone containing por...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00024-3
更新日期:2003-06-01 00:00:00
abstract::Bioactive glasses are potentially useful as bone defect fillers, and vascular endothelial growth factor (VEGF) has demonstrated benefit in bone regeneration as well. We hypothesized that the specific combination of prolonged localized VEGF presentation from a matrix coated with a bioactive glass may enhance bone regen...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.01.033
更新日期:2006-06-01 00:00:00
abstract::The purpose of this work was to study the relationship between water absorption, dimensional change (swelling) under cavity constraint and radial stress generation in resin matrix dental restorative materials. Water absorption was determined on disc specimens whilst swelling was determined on samples of materials rest...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.10.088
更新日期:2004-08-01 00:00:00