Abstract：
INTRODUCTION:The health of human teeth depends on the integrity of the hard tissue and the activity of the pulp and periodontal tissues, which are responsible for nutritional supply. Without the nourishing of the pulp tissue, the possibility of tooth fracture can increase. In immature permanent teeth, root development may be influenced as well. This study explored the potential of using autologous dental pulp stem cells (DPSCs) to achieve pulp regeneration in a canine pulpless model. METHODS:The establishment of the pulpless animal model involved pulp extirpation and root canal preparation of young permanent incisor teeth in beagles. Autologous DPSCs were obtained from extracted first molars and expanded ex vivo to obtain a larger number of cells. The biological characteristics of canine DPSCs (cDPSCs) were analyzed both in vitro and in vivo by using the same method as used in human DPSCs. cDPSCs were transplanted into the pulpless root canal with Gelfoam as the scaffold, and root development was evaluated by radiographic and histologic analyses. RESULTS:cDPSCs with rapid proliferation, multiple differentiation capacity, and development potential were successfully isolated and identified both in vitro and in vivo. After they were transplanted into the pulpless root canal with Gelfoam as the scaffold, DPSCs were capable of generating pulp-like tissues containing blood vessels and dentin-like tissue. Thickening of the root canal wall was also observed. CONCLUSIONS:This study demonstrates the feasibility of using stem cell-mediated tissue engineering to realize pulp regeneration in immature teeth.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wang Y,Zhao Y,Jia W,Yang J,Ge Ldoi
10.1016/j.joen.2012.10.002subject
Has Abstractpub_date
2013-02-01 00:00:00pages
195-201issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(12)00935-1journal_volume
39pub_type
杂志文章abstract：:Pulp regeneration and its clinical translation into regenerative endodontic procedures are receiving increasing research attention, leading to significant growth of the published scientific and clinical literature within these areas. Development of research strategies, which consider patient-, clinician-, and scientis...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.06.002
更新日期：2017-09-01 00:00:00
abstract：:The aim of this study was to describe a guided endodontic technique that facilitates access to root canals of molars presenting with pulp calcifications. A 61-year-old woman presented to our service with pain in the upper left molar region. The second and third left molars showed signs of apical periodontitis confirme...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.02.009
更新日期：2018-05-01 00:00:00
abstract：INTRODUCTION:This case report describes the effect of unintentionally extruded mineral trioxide aggregate (MTA) into periradicular lesions and tissues on the prognosis of periradicular healing in maxillary left central incisor with periradicular lesion. METHODS:A 17-year-old girl with a large periradicular lesion from...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.11.026
更新日期：2010-04-01 00:00:00
abstract：:The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 da...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80460-7
更新日期：1993-06-01 00:00:00
abstract：:A technique utilizing fiberoptics to transilluminate a beveled root surface during periradicular surgery is described. This procedure when integrated with other standard endodontic surgical aids may help diagnose apical variations and vertical root fractures. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80301-8
更新日期：1994-08-01 00:00:00
abstract：INTRODUCTION:Periodontal mechanoreceptors (PMRs) are refined neural receptors present in abundance at the root apex and have a pivotal role in oral fine motor control. This case-control study aimed to evaluate the oral fine motor control of teeth treated with endodontic microsurgery (EMS) in comparison with the control...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2020.10.022
更新日期：2021-02-01 00:00:00
abstract：INTRODUCTION:Different microbial communities have been associated with acute endodontic infections. The majority of the microorganisms are as yet uncultivable or difficult to grow under current laboratory conditions. Treponema species are strict anaerobic bacteria that are involved in several oral diseases. The aim of ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.008
更新日期：2010-11-01 00:00:00
abstract：:The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Gro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200406000-00010
更新日期：2004-06-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of different irrigating solutions on smear layer removal and dentinal tubule opening on root canal surfaces after post space preparation and to study whether additional ultrasonic irrigation has any effect on smear layer removal. Forty-eight anterior teeth were trea...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.01.006
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:Endodontic claims are the most frequently filed malpractice claims in dentistry. The aim of this study was to categorize and review errors and complications related to endodontic procedures that resulted in legal actions against the treating practitioner. METHODS:All dental malpractice complaints reported...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.03.030
更新日期：2010-06-01 00:00:00
abstract：:This study compared a tapered, calibrated spreader/plugger instrument (M-series) to noncalibrated spreaders and pluggers in achieving apical condensation of gutta-percha in 20 single-rooted human teeth. The distances between the tip of the instruments and the tip of the master gutta-percha were measured. Results showe...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80680-1
更新日期：1993-04-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the cell viability of dental pulp cells treated with Biodentine (Septodont, Saint-Maur, France) and mineral trioxide aggregate (MTA) and the in vitro and in vivo expression of mineralization markers induced by the 2 materials. METHODS:Human dental pulp cells isolat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.12.018
更新日期：2016-04-01 00:00:00
abstract：:Root canal bacteria in teeth with apical periodontitis were enumerated after extraction and incubation. Canals in 36 teeth were sampled after: S1, incubation for 2 hours (group A), 2 days (group B), 4 days (group C), and 6 days (group D); S2, subsequent incubation for 1 week; S3, canal disinfection; and S4, final incu...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.08.038
更新日期：2008-12-01 00:00:00
abstract：INTRODUCTION:This study evaluated 4 different light-emitting diode (LED) transilluminators and the impact of operator experience in the detection of dentinal defects through an ex vivo TRUEJAW surgical model (Dental Engineering Laboratories, Santa Barbara, CA). METHODS:Forty-four extracted and endodontically treated m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.10.031
更新日期：2020-02-01 00:00:00
abstract：:The aim of this study was to assess the cytotoxicity of pure eugenol in an in vitro method by diluting it to various concentrations in alcohol and determining the maximum noncytotoxic concentration. We used solutions of eugenol and ethyl alcohol that are soluble in water in any given proportion. The cytotoxicity of th...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80012-4
更新日期：1996-10-01 00:00:00
abstract：:K-type files, Sureflex NiTi files, and FlexoFiles were evaluated morphometrically as they come from the manufacturer, and after being used to instrument root canals 1, 3, and 5 times in maxillary premolars. Evaluation was made using a stereomicroscope at x40 magnification and photomicrographs. The abnormalities found ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(98)80046-0
更新日期：1998-07-01 00:00:00
abstract：:The aim of this study was to investigate the prevalence and configuration of the C-shaped canal using serial axial computed tomography images of the mandibular second molars that had not been restored severely or treated endodontically, and to compare the thickness of the remaining tooth structure from the center of t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.007
更新日期：2006-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this prospective, randomized controlled trial was to evaluate the healing outcomes of platelet-rich fibrin (PRF) in periapical surgeries involving apicomarginal defects and to compare these results with surgeries not using any guided tissue regeneration techniques. METHODS:Thirty patients w...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2015.04.004
更新日期：2015-07-01 00:00:00
abstract：:Reports on the prevalence of Enterococcus faecalis in root canals vary considerably, potentially because of variations in clinical sampling and sample analysis methods. This study compared culture and real-time quantitative polymerase chain reaction (qPCR) to detect and quantify E. faecalis in the same root canal samp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2005.10.037
更新日期：2006-03-01 00:00:00
abstract：INTRODUCTION:This 2-part study hypothesized that a bioactive scaffold containing a sustained transforming growth factor (TGF)-β1-releasing nanoparticle system will promote migration and enhance differentiation of stem cells from the apical papilla (SCAP). The study aimed to develop and characterize a novel modified chi...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.06.017
更新日期：2016-09-01 00:00:00
abstract：INTRODUCTION:The ability to resolve pulpal inflammation to achieve predictable regeneration of the dentin-pulp complex has remained elusive and presents a challenge for clinicians and researchers. Although the dentin-pulp complex can react naturally to injury by forming a bridge of reparative dentin that protects the p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.06.039
更新日期：2020-09-01 00:00:00
abstract：:Removing posts from root canals prior to endodontic retreatment is often a challenge. The Gonon post removing system (Thomas Extracteur De Pivots; FFDM-Pneumat, Bourges, France) is safe and efficient and can be used for anterior, bicuspid, and even molar teeth. The Gonon system is illustrated with one clinical case. ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(89)80200-6
更新日期：1989-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this in vivo study was to evaluate the accuracy of small-volume cone-beam computed tomographic (CBCT) imaging compared with conventional periapical radiography (CPR) in the diagnosis of vertical root fractures (VRFs) using exploratory surgery as the reference standard. METHODS:Eighty-two de...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.04.015
更新日期：2020-08-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare canal transportation and the centering ability of Reciproc (VDW, Munich, Germany), WaveOne (Dentsply Maillefer, Ballaigues, Switzerland), and EdgeFile (EdgeEndo, Albuquerque, NM) rotary systems using cone-beam computed tomographic imaging. METHODS:Ninety mesiobucca...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2018.09.011
更新日期：2018-12-01 00:00:00
abstract：INTRODUCTION:Culture-independent studies have revealed a broad spectrum of oral bacterial taxa that may be associated with disease. This study investigated the prevalence of three new candidate oral pathogens: Prevotella baroniae, Prevotella multisaccharivorax, and as-yet-uncultivated Bacteroidetes oral clone X083 in p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2009.05.033
更新日期：2009-10-01 00:00:00
abstract：INTRODUCTION:This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts. METHODS:Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.11.026
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:A great number of studies performed in the last 3 decades have shown the superior mechanical features of nickel-titanium files as compared with stainless steel files. A novel file system recently developed, Gentlefile, defies these findings and claims to have superior qualities despite the fact it is made ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.12.023
更新日期：2016-03-01 00:00:00
abstract：INTRODUCTION:The mental foramen (MF) is an important landmark in dentistry. Knowledge of its position is central to perform block anesthesia of the mental nerve or to avoid nerve damage during surgical procedures in the premolar area of the mandible. The present radiographic study aimed at evaluating the location and d...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.07.033
更新日期：2013-12-01 00:00:00
abstract：INTRODUCTION:This study evaluated the influence of glide path on canal curvature and axis modification after instrumentation with WaveOne Primary reciprocating files. METHODS:Thirty ISO 15, 0.02 taper Endo Training Blocks were used. In group 1, glide path was created with PathFile 1, 2, and 3 at working length, wherea...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.09.030
更新日期：2012-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the bleaching effect of sodium percarbonate on artificially stained pulpless teeth. Twenty extracted human mandibular premolars were stained by immersing them in human blood mixed with iron sulfide, and divided into three test groups and one control group. The following bleach...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200001000-00006
更新日期：2000-01-01 00:00:00
