Abstract:
:Protein serine/threonine phosphatase-1 (PP-1) is one of the key enzymes responsible for dephosphorylation in vertebrates. Protein dephosphorylation via PP-1 is implicated in many different biological processes including gene expression, cell cycle control, transformation, neuronal transmission, apoptosis, autophage and senescence. However, whether PP-1 directly controls animal development remains to be investigated. Here, we present direct evidence to show that PP-1 plays an essential role in regulating eye development of vertebrates. Using goldfish as a model system, we have shown the following novel results. First, inhibition of PP-1 activity leads to death of a majority of the treated embryos, and the survived embryos displayed severe phenotype in the eye. Second, knockdown of each catalytic subunit of PP-1 with morpholino oligomers leads to partial (PP-lα knockdown) or complete (PP-lβ or PP-lγ knockdown) death of the injected embryos. The survived embryos from PP-1α knockdown displayed clear retardation in lens differentiation. Finally, overexpression of each subunit of PP-1 also causes death of majority of the injected embryos and leads to abnormal development of goldfish eye. Mechanistically, Pax-6 is one of the major downstream targets mediating the effects of PP-1 function since the eye phenotype in Pax-6 knockdown fish is similar to that derived from overexpression of PP-1. Together, our results for the first time provide direct evidence that protein phosphatase-1 plays a key role in governing normal eye formation during goldfish development.
journal_name
Curr Mol Medjournal_title
Current molecular medicineauthors
Liu WB,Yan Q,Liu FY,Tang XC,Chen HG,Liu J,Nie L,Zhang XW,Ji WK,Hu XH,Hu WF,Woodward Z,Wu KL,Wu MX,Liu XL,Luo LX,Yu MB,Liu YZ,Liu SJ,Li DWdoi
10.2174/156652412803833535subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1361-71issue
10eissn
1566-5240issn
1875-5666pii
CMM-EPUB-20120924-1journal_volume
12pub_type
杂志文章abstract:BACKGROUND:Colorectal cancer (CRC) is the third most common cancer worldwide and the third leading cause of cancer-related death. It is a heterogeneous disease that develops through different genetic and epigenetic mechanisms. To date, no comprehensive systematic review investigating genetic risk factors for familial a...
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pub_type: 杂志文章
doi:10.2174/1566524019666191014170136
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更新日期:2009-06-01 00:00:00
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更新日期:2011-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2013-12-01 00:00:00
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更新日期:2009-04-01 00:00:00
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更新日期:2004-12-01 00:00:00
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更新日期:2001-07-01 00:00:00
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更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2010-06-01 00:00:00
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更新日期:2011-03-01 00:00:00
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更新日期:2019-01-01 00:00:00
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更新日期:2018-01-01 00:00:00
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更新日期:2013-05-01 00:00:00