Abstract:
BACKGROUND:Photoreceptor cell death is a key pathology of retinal degeneration diseases. To date, the molecular mechanisms for this pathological process remain largely unclear. Junctional adhesion molecule-c (Jam-c) has been shown to play important roles in different biological events. However, its effect on retinal neuronal cells is unknown. OBJECTIVE:To determine the effect of Jam-c on adult mouse eyes, particularly, on retinal structure, vasculature and photoreceptor cells, in order to explore potential important target molecules for ocular diseases. METHODS:Jam-c global knockout mice, endothelial-specific and neuronal-specific Jam-c conditional knockout mice using Tie2-Cre and Nestin-Cre mice respectively were used in this study. Mouse eyes were harvested from the different groups and eye size examined. Cryosections of the eyes were made and stained with Hematoxylin and Eosin (H&E) and the thicknesses of retinal layers measured. Retinal blood vessels and cone and rod photoreceptors were analyzed using isolectin B4, peanut agglutinin and rhodopsin as markers respectively. In vivo Jam-c knockdown in mouse eyes was performed by intravitreal injection of Jam-c shRNA. Jam-c expression in the retinae was quantified by real-time PCR. RESULTS:Global Jam-c gene deletion in mice resulted in smaller eyes and decreased the diameters of lens and iris. Jam-c-/- mice display marked thinning of the outer nuclear layer (ONL), less numbers of photoreceptor cells, and abnormal retinal vasculature. Importantly, neuronal-specific Jam-c deletion led to similar phenotype, whereas no obvious defect was observed in endothelial-specific Jam-c knockout mice. Moreover, Jam-c knockdown by shRNA also decreased ONL thickness and photoreceptor numbers. CONCLUSION:We found that Jam-c is critically required for the normal size and retinal structure. Particularly, Jam-c plays important roles in maintaining the normal retinal thickness, vasculature and photoreceptor numbers. Jam-c thus may therefore have important roles in various ocular diseases.
journal_name
Curr Mol Medjournal_title
Current molecular medicineauthors
Li Y,Zhang F,Lu W,Li Xdoi
10.2174/1566524018666180212144500subject
Has Abstractpub_date
2018-03-09 00:00:00pages
497-508issue
7eissn
1566-5240issn
1875-5666pii
CMM-EPUB-88485journal_volume
17pub_type
杂志文章abstract:PURPOSE:To evaluate cytokine expression profile in aqueous humor and sera in patients with HLAB27 associated acute anterior uveitis (AAU) and idiopathic AAU. METHODS:Twenty patients with AAU and 17 controls were recruited from August 2012 to March 2013. Study subjects with uveitis were divided into two groups: 9 patie...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524015666150731100012
更新日期:2015-01-01 00:00:00
abstract::Asthma, chronic obstructive pulmonary disorder (COPD), and cystic fibrosis (CF), chronic diseases of the airways, are characterized by symptoms such as inflammation of the lung tissue, mucus hypersecretion, constriction of the airways, and excessive fibrosis of airway tissue. Transforming growth factor (TGF)-beta, a c...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408785160943
更新日期:2008-08-01 00:00:00
abstract::Several species of kinetoplastid protozoa cause major human infectious diseases. Trypanosoma cruzi is responsible for the fatal Chagas disease in large parts of South America, the various species of Leishmania cause a number of different human diseases with millions of patients world-wide, and the African trypanosome ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524043360113
更新日期:2004-09-01 00:00:00
abstract::In this study, a two-dimensional gel-based proteomic approach was applied to profile the protein alterations underlying the significant adverse effects from post-stroke depression (PSD). In view of the close association between left prefrontal cortical dysfunction and PSD, a PSD rat model was constructed through a lef...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524014666141021143333
更新日期:2014-01-01 00:00:00
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journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524014666140414211223
更新日期:2014-05-01 00:00:00
abstract::N-methyl-D-aspartate (NMDA) receptor plays important roles in learning and memory. NMDA receptors are a tetramer that consists of two glycine-binding subunits GluN1, two glutamate-binding subunits (i.e., GluN2A, GluN2B, GluN2C, and GluN2D), a combination of a GluN2 subunit and glycine-binding GluN3 subunit (i.e., GluN...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524015666150330142807
更新日期:2015-01-01 00:00:00
abstract::HMGB1/Amphoterin is a ubiquitous, highly conserved DNA-binding protein that can be also released to the extracellular space by various cell types. Extracellular HMGB1 regulates migratory responses of several cell types through binding to RAGE that communicates with the cytoskeleton to regulate cell motility. HMGB1-ind...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652407783220750
更新日期:2007-12-01 00:00:00
abstract::Glycogen is a branched polymer of glucose which serves as a reservoir of glucose units. The two largest deposits in mammals are in the liver and skeletal muscle but many cells are capable synthesizing glycogen. Its accumulation and utilization are under elaborate controls involving primarily covalent phosphorylation a...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524024605761
更新日期:2002-03-01 00:00:00
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journal_title:Current molecular medicine
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更新日期:2001-05-01 00:00:00
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journal_title:Current molecular medicine
pub_type: 临床试验,杂志文章
doi:10.2174/1566524018666181004121604
更新日期:2018-01-01 00:00:00
abstract::There is growing concern that gastrointestinal exposure to particles is associated with increased risk of toxicity to internal organs and carcinogenicity. The mechanism of action is related to particle-induced oxidative stress and oxidation of DNA. Observations from animal models indicate that gastrointestinal exposur...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652412800792624
更新日期:2012-07-01 00:00:00
abstract::Crimean-Congo hemorrhagic fever virus (CCHFV) is an important human pathogen, which is the cause of a tick-borne illness occurring in many areas of Africa, Asia, and Europe. CCHF is characterized by a sudden onset of high fever, chills, and severe headache. Other symptoms can include gastrointestinal disorders, such a...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
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更新日期:2005-12-01 00:00:00
abstract::Nitric oxide has emerged as one of the most important and diverse players in physiology. This small diatomic radical stunned researchers because of its existence and unique biological properties in human physiology. Over the last two decades it was found that NO often has fickle behavior in pathophysiological mechanis...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
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更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Dynamic epigenetic alterations accompanying CD4+ T helper cell differentiation have been implicated in multiple autoimmune diseases. The bromodomain and extra-terminal (BET) proteins are epigenetic regulators that recognize and bind to acetylated histones in chromatin and are targets for pharmacological inhi...
journal_title:Current molecular medicine
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doi:10.2174/1566524019666190126112238
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:In this study, the antioxidant property of new synthesized azomethins has been investigated as theoretical and experimental. METHODS AND RESULTS:Density functional theory (DFT) was employed to investigate the Bond Dissociation Enthalpy (BDE), Mulliken Charges, NBO analysis, Ionization Potential (IP), Electr...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524019666190509102620
更新日期:2019-01-01 00:00:00
abstract::Islet cell transplantation is an attractive alternative therapy to conventional insulin treatment or vascularized whole pancreas transplantation for type 1 diabetic patients. It represents a successful example of somatic cell therapy in humans based on complex procedures for islet isolation from whole pancreas. The is...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652406777435453
更新日期:2006-06-01 00:00:00
abstract::Preeclampsia is a disease of high incidence in pregnant women which complicates pregnancy and may lead to the death of mother and baby. Preeclampsia is characterized by a series of clinical features such as hypertension and proteinuria associated with endothelial dysfunction. Although the causes of disease have not be...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/15665240113139990058
更新日期:2013-09-01 00:00:00
abstract::WHIM syndrome is a dominantly inherited primary immunodeficiency disorder representing the first identified example of human disease caused by mutations in the gene encoding for the chemokine receptor CXCR4. Pathogenesis is mediated by CXCR4 hyperfunction, leading to increased responsiveness to its unique ligand CXCL1...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
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更新日期:2011-06-01 00:00:00
abstract::Deregulation of the PI3K pathway is common in human cancer. The basic players in this pathway are the kinases PI3K and AKT and the phosphatase PTEN. This review will summarize some of the key animal models that have helped us understand this signaling network and its contribution to tumorigenesis. Recently, great adva...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409788488766
更新日期:2009-06-01 00:00:00
abstract:PURPOSE:A small molecular compound, aminooxy-acetic acid (AOA), has been shown to modulate experimental autoimmune encephalomyelitis (EAE). The current study was designed to investigate whether AOA has a similar effect on the development of experimental autoimmune uveitis (EAU) and to further explore underlying mechani...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524020666200211112219
更新日期:2020-01-01 00:00:00
abstract::Traditionally regarded as a genetic disease of the cardiac sarcomere, hypertrophic cardiomyopathy (HCM) is the most common inherited cardiovascular disease and a significant cause of sudden cardiac death. While the most common etiologies of this phenotypically diverse disease lie in a handful of genes encoding critica...
journal_title:Current molecular medicine
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
abstract::p66Shc is the only known proapoptotic member of the Shc protein family of molecular adaptors. Through its redox activity, p66Shc oxidates cytochrome-c, leading to increased ROS production and, eventually, to apoptosis. p66Shc has been implicated in the control of oxidative stress and life span in mammals. In this revi...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409787847254
更新日期:2009-04-01 00:00:00
abstract::Many potential HIV vaccine strategies are being explored in both animal model and human settings. The success of any vaccine relies on relevant antigenic determinants being presented to the immune system for the activation of broad and long-lasting immunity. Effective immunity against HIV infection will likely require...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524033479870
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Oxidative stress and chronic hyperglycemia are two major side effects of type 2 diabetes affecting all cell types including mesenchymal stem cells (MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial information for efficient treatment. METHODS:Placental mesenchymal stem...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524019666190722115842
更新日期:2019-01-01 00:00:00
abstract::Since the discovery almost fifteen years ago that E2F transcription factors are key targets of the retinoblastoma protein (RB), studies of the E2F family have uncovered critical roles in the control of transcription, cell cycle and apoptosis. E2F proteins are encoded by at least eight genes, E2F1 through E2F8. While s...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524010606070739
更新日期:2006-11-01 00:00:00
abstract::Non-invasive energy metabolism measurements in brain tumors in vivo are now performed widely as molecular imaging by positron emission tomography. This capability has developed from a large number of basic and clinical science investigations that have cross fertilized one another. Apart from precise anatomical localiz...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408786733766
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Osteoporosis is a systemic skeletal disease characterized by low bone mineral density. Vitamin D metabolism may play a pivotal role in its pathophysiology. OBJECTIVES:To determine the association between Vitamin D receptor gene polymorphisms and bone density, as well as its relation to biochemical markers o...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524019666190409122155
更新日期:2019-01-01 00:00:00
abstract::The generation of functional neural progenitor cells (NPCs) independent of donor brain tissue and embryonic tissues is of great therapeutic interest with regard to regenerative medicine and the possible treatment of neurodegenerative disorders. Traditionally, NPCs are derived through the differentiation of embryonic s...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/156652412798889018
更新日期:2012-02-01 00:00:00
abstract::Ca2+ ions are involved in the regulation of many diverse functions in animal and plant cells, e.g. muscle contraction, secretion of neurotransmitters, hormones and enzymes, fertilization of oocytes, and lymphocyte activation and proliferation. The intracellular Ca2+ concentration can be increased by different molecula...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524024605707
更新日期:2002-05-01 00:00:00
abstract::Chemokines are newly discovered molecules that mediate the migration of leukocytes into inflammed tissues and control the inflammatory reactions in various immune-mediated diseases. Both in animal models and in human specimens, chemokine expression is associated with atherosclerotic lesion development and vascular rem...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524033361546
更新日期:2003-03-01 00:00:00