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The potassium channel opener CGS7184 activates Ca²⁺ release from the endoplasmic reticulum.

Abstract：

:CGS7184 (ethyl 1-[[(4-chlorophenyl)amino]oxo]-2-hydroxy-6-trifluoromethyl-1H-indole-3-carboxylate) is a synthetic large-conductance Ca(2+)-activated potassium (BK(Ca)) channel opener. The existing literature suggests that potassium channels are involved in cardioprotection, particularly during ischemia-reperfusion events. However, the cellular mechanisms mediating the effects of CGS7184 remain unclear. In the present study, we investigated the effect of the BK(Ca) channel opener CGS7184 on Ca(2+) homeostasis in H9C2 and C2C12 cell lines, Ca(2+) uptake by isolated sarcoplasmic reticulum (SR) vesicles, SR Ca(2+)-ATPase (SERCA) activity, and single-channel properties of the ryanodine receptor calcium release channel (RYR2) when incorporated into a planar lipid bilayer. The effects of CGS7184 on calcium homeostasis in C2C12 and H9C2 cell lines were measured with a Fura-2 fluorescent indicator. The BK(Ca) channel opener CGS7184, when added to the H9C2 and C2C12 cells, caused a concentration-dependent release of calcium from internal stores. Calcium accumulation by the SR vesicles isolated from cardiac and skeletal muscle was inhibited by CGS7184 with a half-maximal inhibition value of 0.45 ± 0.04 μM and 0.37 ± 0.03 μM, respectively. The results of the present study indicate that the BK(Ca) channel opener CGS7184 modulates cytosolic Ca(2+) concentration in H9C2 and C1C12 cells due to its interaction with the endoplasmic reticulum (ER). CGS7184 approximately doubled the opening probability of RYR2 channels; however, the compound seemed to most strongly affect channels with a higher control activity. These results strongly suggest that the BK(Ca) channel opener CGS7184 affects intracellular calcium homeostasis by interacting with the sarcoplasmic reticulum RYR2 channels.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Wrzosek A,Tomaskova Z,Ondrias K,Lukasiak A,Szewczyk A

doi

10.1016/j.ejphar.2012.06.029

subject

Has Abstract

pub_date

2012-09-05 00:00:00

pages

60-7

issue

1-3

eissn

0014-2999

issn

1879-0712

pii

S0014-2999(12)00541-9

journal_volume

690

pub_type

杂志文章

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