Molecular characterization, polymorphism and association of porcine SPATA19 gene.

Abstract:

:Spermatogenesis associated 19 (SPATA19) is an important reproduction related gene. In this study, we cloned the full-length cDNA sequence of porcine SPATA19 gene through the rapid amplification of cDNA ends (RACE) method. The porcine SPATA19 gene encodes a protein of 154 amino acids which shares high homology with the SPATA19 of ten species: giant panda (87 %), dog (86 %), cattle (84 %), rabbit (78 %), sumatran orangutan (72 %), human (71 %), rhesus monkey (71 %), chimpanzee (70 %), mouse (71 %) and rat (69 %). The phylogenetic analysis revealed that the porcine SPATA19 gene has a closer genetic relationship with the SPATA19 gene of dog. This gene is structured in six exons and five introns as revealed by computer-assisted analysis. PCR-RFLP was established to detect the GU475012:c.515T>C substitution of porcine SPATA19 gene mRNA and association of this mutation with litter size traits was assessed in Large White (n = 100) and Landrace (n = 100) pig populations. Results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White sows and Landrace sows. Therefore, SPATA19 gene could be an useful candidate gene in selection for increasing litter size in pigs. These data serve as a foundation for further insight into this novel porcine gene.

journal_name

Mol Biol Rep

authors

Wang JY,Lan J,Zhao J,Chen L,Liu Y

doi

10.1007/s11033-012-1839-x

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

9741-6

issue

10

eissn

0301-4851

issn

1573-4978

journal_volume

39

pub_type

杂志文章
  • Green fluorescent protein is superior to blue fluorescent protein as a quantitative reporter of promoter activity in E. coli.

    abstract::Fluorescent proteins related to and derived from green fluorescent protein (GFP) are widely used as tools for investigating a wide range of biological processes. In particular, GFP and its relatives have been used extensively as qualitative reporters of gene expression in many different organisms, but relatively few s...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9285-5

    authors: Lissemore JL,Bayes J,Calvey M,Reineke L,Colagiavanni A,Tscheiner M,Mascotti DP

    更新日期:2009-05-01 00:00:00

  • Molecular phylogeny and biogeography of lac insects (Hemiptera: Kerriidae) inferred from nuclear and mitochondrial gene sequences.

    abstract::Lac insects are commercial scale insects with high economic value. The combined molecular phylogeny of 20 lac insect populations was generated using elongation factor 1 alpha, mitochondrial cytochrome c oxidase subunit I and the small subunit ribosomal RNA gene loci. The 20 populations of lac insects clustered into fo...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2701-5

    authors: Chen H,Chen X,Feng Y,Yang H,He R,Zhang W,Yang Z

    更新日期:2013-10-01 00:00:00

  • The Cdx-2 polymorphism in the VDR gene is associated with increased risk of cancer: a meta-analysis.

    abstract::The Cdx-2 polymorphism in VDR gene has been extensively investigated for association with cancer risk, however, results of different studies have been inconsistent. The objective of this study is to assess the relationship of the Cdx-2 polymorphism in VDR and cancer risk by meta-analysis. All eligible case-control stu...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-013-2503-9

    authors: Huang J,Huang J,Ma Y,Wang H,Yang J,Xiong T,Du L

    更新日期:2013-07-01 00:00:00

  • The role of CcTpt1 in scale and early embryo development in common carp (Cyprinus carpio, Cyprinidae).

    abstract::The full length cDNA sequence of the Tpt1/TCTP (Tumor protein, Translationally-controlled1) gene was identified from Common Carp (Cyprinus carpio, Cyprinidae), and was designated as CcTpt1 gene. The CDS is 510 bp and encodes a 170-amino acid peptide with a typical Tpt1 signature 2 domain, and is a typical Tpt1 protein...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2806-x

    authors: Jiang L,Wang Y,Cheng A,Zhang B,Ma L,Liu Y,Sun X

    更新日期:2013-10-13 00:00:00

  • Blocking P2X7 receptor ameliorates oxidized LDL-mediated podocyte apoptosis.

    abstract::The purpose of our research is to elucidate whether oxLDL activates P2X7R in cultured human podocytes and if the activation of P2X7R leads to podocyte apoptosis. Additionally, we explore the underlying mechanism involved in podocyte apoptosis. Immortalized human podocytes were incubated with oxLDL (80 µg/ml), P2X7R an...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04823-6

    authors: Zhu Y,Li Q,Xun W,Chen Y,Zhang C,Sun S

    更新日期:2019-08-01 00:00:00

  • The association of polymorphisms on TGFBR1 and colorectal cancer risk: a meta-analysis.

    abstract::Epidemiological studies found inconsistent results on the association of two variants on TGFBR1 (TGFBR1*6A and Int7G24A) with colorectal cancer (CRC) risk. The present study was aimed to evaluate the association of these two variants with CRC susceptibility via the meta-analysis methods. For variant TGFBR1*6A, nine re...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-011-1009-6

    authors: Zhang X,Wu L,Sheng Y,Zhou W,Huang Z,Qu J,Gao G,Cai D,Zhang M

    更新日期:2012-03-01 00:00:00

  • Three mRNA populations differing in turnover and processing in mouse liver.

    abstract::Using kinetics of approach to steady state labeling, we have found that liver poly(A)+ mRNAs fall into three populations, differing in stability and probably in processing, as reflected in their dissimilar delays in reaching polyribosomes and turnover times. There are mRNA-1 (delay 10 min, half-life 1 hr); mRNA-2(dela...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00357013

    authors: Lubimova EV,Chernovskaja TV,Lerman MI

    更新日期:1975-12-01 00:00:00

  • Proangiogenic signature in advanced laryngeal carcinoma after microRNA expression profiling.

    abstract::The aim of this study is to evaluate which dysregulated angiomiRs compose the specific proangiogenic microRNA signature of advanced laryngeal cancer and review the literature. Thirty-six samples from twelve patients with advanced laryngeal carcinoma were collected. Total RNA was extracted and microRNA global profiling...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05250-8

    authors: Popov TM,Giragosyan S,Petkova V,Stancheva G,Marinov T,Belitova M,Rangachev J,Popova D,Kaneva RP

    更新日期:2020-07-01 00:00:00

  • Temporal expression profiles of ceramide and ceramide-related genes in wild-type and mPer1/mPer2 double knockout mice.

    abstract::Most living organisms exhibit circadian rhythms in physiology and behavior. These oscillations are generated by an endogenous circadian clock and control many biological processes. Ceramide has attracted attention as a signal mediator in diverse cell processes including cell death and differentiation. The relationship...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1207-2

    authors: Jang YS,Kang YJ,Kim TJ,Bae K

    更新日期:2012-04-01 00:00:00

  • (-)-Epigallocatechin-gallate (EGCG) stabilize the mitochondrial enzymes and inhibits the apoptosis in cigarette smoke-induced myocardial dysfunction in rats.

    abstract::The present study brings out the preventive role of (-)-epigallocatechin-gallate (EGCG) on cardiac mitochondrial metabolism and apoptosis in cigarette smoke (CS)-exposed rats. The CS-exposed rats showed significantly decreased activities of TCA cycle enzymes and mitochondrial enzymatic antioxidants, on the other hand,...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2673-5

    authors: Adikesavan G,Vinayagam MM,Abdulrahman LA,Chinnasamy T

    更新日期:2013-12-01 00:00:00

  • Location of RNase and RNase inhibitor on free cytoplasmic mRNA-protein particles from human placenta.

    abstract::Free cytoplasmic mRNPs were isolated from human placenta. An activity of RNase was associated with these particles but was mostly inhibited by a labile protein inhibitor. Both RNase and RNase inhibitor were extractable from mRNPs by 0.5 M KCl. The nature of the association of the RNase-RNase inhibitor complex with mRN...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00775355

    authors: Gileadi O,Lorberboum H,de Groot N,Hochberg AA

    更新日期:1984-01-01 00:00:00

  • Effect of BRAF V600E mutation on tumor response of anti-EGFR monoclonal antibodies for first-line metastatic colorectal cancer treatment: a meta-analysis of randomized studies.

    abstract::Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDL...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-013-2974-8

    authors: Cui D,Cao D,Yang Y,Qiu M,Huang Y,Yi C

    更新日期:2014-03-01 00:00:00

  • New uracil analogs as downregulators of ABC transporters in 5-fluorouracil-resistant human leukemia HL-60 cell line.

    abstract::Overexpression of ATP-binding cassette (ABC) transporters causing multidrug resistance (MDR) in cancer cells is one of the major obstacles in cancer chemotherapy. The 5-FU resistant subclone (HL-60/5FU) of the human HL-60 promyelocytic leukemia cell line was selected by the conventional method of continuous exposure o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05017-w

    authors: Długosz-Pokorska A,Pięta M,Janecki T,Janecka A

    更新日期:2019-12-01 00:00:00

  • Refined definition of the 56K and other autoantigens in the 50-60 kDa region.

    abstract::Alteration of the acrylamide:bisacrylamide ratio in the SDS-polyacrylamide gel used for Western blotting strongly improved the unambiguous detection of antibodies against 50-60 kDa autoantigens present in autoimmune patient sera. The relative migration of Ro52, the 56K autoantigen and calreticulin increased with reduc...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00419667

    authors: Pruijn GJ,Bozic B,Schoute F,Rokeach LA,van Venrooij WJ

    更新日期:1992-09-01 00:00:00

  • Identification of candidate genes associated with CBB resistance in common bean HR45 (Phaseolus vulgaris L.) using cDNA-AFLP.

    abstract::Common bacterial blight (CBB), incited by Xanthomonas axonopodis pv. phaseoli (Xap), is a serious seed-borne disease of common bean (Phaseolus vulgaris L.) in both temperate and tropical production zones. The line HR45 is highly resistant to Xap infection on leaves and pods in both field and greenhouse. To understand ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0079-1

    authors: Shi C,Chaudhary S,Yu K,Park SJ,Navabi A,McClean PE

    更新日期:2011-01-01 00:00:00

  • Down regulation of ITGA4 and ITGA5 genes after formation of 3D spherules by human Wharton's jelly stem cells (hWJSCs).

    abstract::Human Wharton's jelly mesenchymal stem cells (hWJSCs) are multipotent stem cells that could be aggregated into 3D spherules. ITGA4 and ITGA5 genes encode α4 and α5 subunits of integrins, respectively. In this study, we analyzed expression levels of ITGA4 and ITGA5 gene mRNAs in undifferentiated and 3D spherules formin...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4157-0

    authors: Mostafavi-Pour Z,Ashrafi MR,Talaei-Khozani T

    更新日期:2018-06-01 00:00:00

  • Complement factor H Y402H gene polymorphism and coronary heart disease susceptibility: a meta-analysis.

    abstract::The complement factor H (CFH) Y402H (T1277C) gene polymorphism has been reported to be associated with coronary heart disease (CHD), but results were conflicting. To evaluate the role of the variant in CHD, we performed meta-analyses of all available data. Both electronic and manual searches were performed, all releva...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-010-9956-x

    authors: Zhang HF,Wang JF,Wang Y,Zhu LG,Lei L

    更新日期:2011-06-01 00:00:00

  • Genetic diversity and maternal origin of Bangladeshi chicken.

    abstract::Local domestic chicken populations are of paramount importance as a source of protein in developing countries. Bangladesh possesses a large number of native chicken populations which display a broad range of phenotypes well adapted to the extreme wet and hot environments of this region. This and the fact that wild jun...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2522-6

    authors: Bhuiyan MS,Chen S,Faruque S,Bhuiyan AK,Beja-Pereira A

    更新日期:2013-06-01 00:00:00

  • Genome-wide analysis of BURP domain-containing genes in maize and sorghum.

    abstract::BURP domain-containing genes comprise a large plant-specific family, yet the functions are very poorly understood, especially in maize (Zea mays) and sorghum (Sorghum vulgare). In this study, 26 BURP family genes in maize (ZmBURP01-15) and sorghum (SbBURP01-11) were identified including the gene structure, phylogeneti...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0587-z

    authors: Gan D,Jiang H,Zhang J,Zhao Y,Zhu S,Cheng B

    更新日期:2011-10-01 00:00:00

  • Sequence variation in promoter regions of genes for CC chemokine ligands (CCL)19 and 21 in Czech patients with myocardial infarction.

    abstract::Recruitment of inflammatory cells to the arterial wall is an important pathogenic mechanism of atherosclerosis and coronary artery disease (CAD). Functional variability in the genes encoding for chemokines that promote infiltration of atherosclerotic plaques by macrophages and lymphocytes may therefore contribute to t...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3175-9

    authors: Stahelova A,Petrkova J,Petrek M,Mrazek F

    更新日期:2014-05-01 00:00:00

  • Inhibiting UHRF1 expression enhances radiosensitivity in human esophageal squamous cell carcinoma.

    abstract::Radiotherapy is an effective treatment for some esophageal cancers, but the molecular mechanisms of radiosensitivity remain unknown. Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a novel nuclear protein which is overexpressed in various cancers but not yet examined in esophageal squamous cell carcinoma ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2559-6

    authors: Yang C,Wang Y,Zhang F,Sun G,Li C,Jing S,Liu Q,Cheng Y

    更新日期:2013-09-01 00:00:00

  • Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A.

    abstract::Mucopolysaccharidosis IV A (MPS IV A) is a lysosomal storage disease produced by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme. Although genotype-phenotype correlations have been reported, these approaches have not enabled to establish a complete genotype-phenotype correlation, and they ha...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3383-3

    authors: Olarte-Avellaneda S,Rodríguez-López A,Alméciga-Díaz CJ,Barrera LA

    更新日期:2014-11-01 00:00:00

  • Down-regulation of platelet-derived growth factor-D expression blockades NF-κB pathway to inhibit cell proliferation and invasion as well as induce apoptosis in esophageal squamous cell carcinoma.

    abstract::Substantial evidence has demonstrated that platelet-derived growth factor-D (PDGF-D) is tightly associated with the development and progression of tumors. However, its biological functions in esophageal squamous cell carcinoma (ESCC) remain to be delineated. In this study, we found that expressions of PDGF-D mRNA and ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2328-y

    authors: Han Y,Guo XH,Zheng QF,Zhu YL,Fan YY,Zhang XY

    更新日期:2013-03-01 00:00:00

  • KIR gene presence/absence polymorphisms and global diversity in the Kirgiz ethnic minority and populations distributed worldwide.

    abstract::Killer cell immunoglobulin like receptor genes expressed by the natural killer cells and T cells of some subclasses are one of the very diversity and complex gene families on chromosome 19q13.4 which play key developmental role in the fight against viral infections, malignantly transformed cells and so on in the first...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4563-3

    authors: Wang HD,Jin XY,Guo YX,Zhang Q,Zhang YW,Wang X,Zhu BF

    更新日期:2019-02-01 00:00:00

  • Evaluation of TRPM (transient receptor potential melastatin) genes expressions in myocardial ischemia and reperfusion.

    abstract::In the present study, the expression levels of TRPM1, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, and TRPM8 genes were evaluated in heart tissues after ischemia/reperfusion (IR). For this study, 30 albino male Wistar rats were equally divided into three groups as follows: Group 1: control group (n:10), Group II: ischemi...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3139-0

    authors: Demir T,Yumrutas O,Cengiz B,Demiryurek S,Unverdi H,Kaplan DS,Bayraktar R,Ozkul N,Bagcı C

    更新日期:2014-05-01 00:00:00

  • SSR markers: a tool for species identification in Psidium (Myrtaceae).

    abstract::Molecular DNA markers are used for detection of polymorphisms in individuals. As they are independent of developmental stage of the plant and environmental influences, they can be useful tools in taxonomy. The alleles of simple sequence repeat (SSR) markers (or microsatellites) are traditionally used to identify taxon...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-015-3927-1

    authors: Tuler AC,Carrijo TT,Nóia LR,Ferreira A,Peixoto AL,da Silva Ferreira MF

    更新日期:2015-11-01 00:00:00

  • Transcription start sites and usage of the first exon of mouse Foxp3 gene.

    abstract::Master differentiation transcription factors (MDFs) play decisive roles in cell lineage commitment and cellular functions. Forkhead box P3 (Foxp3) is the MDF essential for the lineage commitment and the suppression function of regulatory T cells (Tregs), which play critical roles in suppression of autoimmunity. Here, ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1825-3

    authors: Fujita T,Fujii H

    更新日期:2012-10-01 00:00:00

  • Molecular cloning, polymorphism and association analyses of a novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs.

    abstract::The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel mRNA that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9326-0

    authors: Liu GY,Xiong YZ

    更新日期:2009-07-01 00:00:00

  • Sensitivity of ortho- and paramyxovirus replication to human interferon alpha.

    abstract::Replication of the influenza virus strains Influenza Ao/WSN (H0N1), fowl plague (Hav1N1) and B-Lee/40 (ATCC) and the paramyxovirus, New Castle disease virus (Victoria) are highly sensitive to human interferon type alpha in Madin Darby bovine kidney cells. Pretreatment of cells with human interferon type alpha resulted...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00775982

    authors: Strube M,Bodo G,Jungwirth C

    更新日期:1985-10-01 00:00:00

  • The human lysyl oxidase-like 2 protein functions as an amine oxidase toward collagen and elastin.

    abstract::The lysyl oxidase-like 2 (LOXL2) protein is a human paralogue of lysyl oxidase (LOX) that functions as an amine oxidase for formation of lysine-derived cross-links found in collagen and elastin. In addition to the C-terminal domains characteristic to the LOX family members, LOXL2 contains four scavenger receptor cyste...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0088-0

    authors: Kim YM,Kim EC,Kim Y

    更新日期:2011-01-01 00:00:00