Perilipin family members preferentially sequester to either triacylglycerol-specific or cholesteryl-ester-specific intracellular lipid storage droplets.

abstract：:The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply the driving force behind the formation and elongation. We tried to clarify how signals in response to extrace...

journal_title：Journal of cell science

authors： Nozumi M,Nakagawa H,Miki H,Takenawa T,Miyamoto S

更新日期：2003-01-15 00:00:00

abstract：:Cysteine proteases are involved in the degradation of intracellular and extracellular proteins, although their precise roles in vivo are not well understood. Here we characterise a genetic mutant of the Caenorhabditis elegans cathepsin L protease gene cpl-1. CPL-1 is provided maternally and is essential for C. elegans...

journal_title：Journal of cell science

authors： Britton C,Murray L

更新日期：2004-10-01 00:00:00

abstract：:In mammals, fertilization initiates Ca2+ oscillations in metaphase II oocytes, which are required for the activation of embryo development. Germinal vesicle (GV) oocytes also display Ca2+ oscillations, although these unfold spontaneously in the absence of any known agonist(s) and their function remains unclear. We fou...

journal_title：Journal of cell science

authors： Wakai T,Fissore RA

更新日期：2019-02-12 00:00:00

abstract：:Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...

journal_title：Journal of cell science

authors： Racchetti G,Lorusso A,Schulte C,Gavello D,Carabelli V,D'Alessandro R,Meldolesi J

更新日期：2010-01-15 00:00:00

abstract：:Hematopoietic and vascular endothelial cells constitute the circulatory system and are both generated from the ventral mesoderm. However, the molecules and signaling pathways involved in ventral mesoderm formation and specification remain unclear. We found that zebrafish etv5a was expressed in the ventral mesoderm dur...

journal_title：Journal of cell science

authors： Chen SY,Shih HY,Lin SJ,Hsiao CD,Li ZC,Cheng YC

更新日期：2013-12-15 00:00:00

abstract：:In 1993, a Commentary in this journal described how a simple mechanical model of cell structure based on tensegrity architecture can help to explain how cell shape, movement and cytoskeletal mechanics are controlled, as well as how cells sense and respond to mechanical forces (J. Cell Sci. 104, 613-627). The cellular ...

journal_title：Journal of cell science

authors： Ingber DE

更新日期：2003-04-01 00:00:00

abstract：:Actin-filament disassembly is crucial for actin-based motility, to control filament network architecture and to regenerate subunits for assembly. Here, we examined the roles of three actin cytoskeletal proteins, coronin, cofilin and Aip1, which have been suggested to combine in various ways to control actin dynamics b...

journal_title：Journal of cell science

authors： Lin MC,Galletta BJ,Sept D,Cooper JA

更新日期：2010-04-15 00:00:00

abstract：:Mammalian Delta9 stearoyl-CoA desaturase 1 (SCD1) is a key enzyme in the biosynthesis of mono-unsaturated fatty acids in the endoplasmic reticulum (ER). It is a short-lived multispanning ER membrane protein, reported to be degraded by the ubiquitin-proteasome-independent pathway. We have examined SCD1 protein degradat...

journal_title：Journal of cell science

authors： Kato H,Sakaki K,Mihara K

更新日期：2006-06-01 00:00:00

abstract：:The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...

journal_title：Journal of cell science

authors： Kim JY,Morgan M,Kim DG,Lee JY,Bai L,Lin Y,Liu ZG,Kim YS

更新日期：2011-02-15 00:00:00

abstract：:The Schizosaccaromyces pombe protein Flp1p belongs to a conserved family of serine-threonine-phosphatases. The founding member of this family, Saccharomyces cerevisiae Cdc14p, is required for inactivation of mitotic CDKs and reversal of CDK mediated phosphorylation at the end of mitosis, thereby bringing about the M-G...

journal_title：Journal of cell science

authors： Esteban V,Blanco M,Cueille N,Simanis V,Moreno S,Bueno A

更新日期：2004-05-15 00:00:00

abstract：:Autophagy is a pivotal cytoprotective process that secures cellular homeostasis, fulfills essential roles in development, immunity and defence against pathogens, and determines the lifespan of eukaryotic organisms. However, autophagy also crucially contributes to the development of age-related human pathologies, inclu...

journal_title：Journal of cell science

authors： Proikas-Cezanne T,Takacs Z,Dönnes P,Kohlbacher O

更新日期：2015-01-15 00:00:00

abstract：:Alpha-satellite (alphoid) DNA is necessary for de novo formation of human artificial chromosomes (HACs) in human cultured cells. To investigate the relationship among centromeric, transcriptionally permissive and non-permissive chromatin assemblies on de novo HAC formation, we constructed bacterial artificial chromoso...

journal_title：Journal of cell science

authors： Nakashima H,Nakano M,Ohnishi R,Hiraoka Y,Kaneda Y,Sugino A,Masumoto H

更新日期：2005-12-15 00:00:00

abstract：:Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...

journal_title：Journal of cell science

authors： Gonsior SM,Platz S,Buchmeier S,Scheer U,Jockusch BM,Hinssen H

更新日期：1999-03-01 00:00:00

abstract：:Kalirin, one of the few Rho guanine nucleotide exchange factors (GEFs) that contains spectrin-like repeats, plays a critical role in axon extension and maintenance of dendritic spines. PC12 cells were used to determine whether Cdk5, a critical participant in both processes, regulates the action of Kalirin. Expression ...

journal_title：Journal of cell science

authors： Xin X,Wang Y,Ma XM,Rompolas P,Keutmann HT,Mains RE,Eipper BA

更新日期：2008-08-01 00:00:00

abstract：:Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril assembly, but the mechanism of actin regulation in striated muscle is ...

journal_title：Journal of cell science

authors： Nomura K,Ono K,Ono S

更新日期：2012-09-01 00:00:00

abstract：:Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics of single CUG-repe...

journal_title：Journal of cell science

authors： Querido E,Gallardo F,Beaudoin M,Ménard C,Chartrand P

更新日期：2011-05-15 00:00:00

abstract：:Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor ...

journal_title：Journal of cell science

authors： Prasad S,Ponimaskin E,Zeug A

更新日期：2019-08-23 00:00:00

abstract：:The neuron specific Drosophila ELAV protein belongs to the ELAV family of RNA binding proteins which are characterized by three highly conserved RNA recognition motifs, an N-terminal domain, and a hinge region between the second and third RNA recognition motifs. Despite their highly conserved RNA recognition motifs th...

journal_title：Journal of cell science

authors： Yannoni YM,White K

更新日期：1999-12-01 00:00:00

abstract：:Triton X-100-treated sperms were injected into non-nucleate egg fragments of Xenopus laevis to determine whether the structure of the injected sperm nucleus is affected by the cyclic activity of the cytoplasm. Swollen vesicular nuclei were very frequently observed when the sperms were injected and incubated during the...

journal_title：Journal of cell science

authors： Sakai M,Shinagawa A

更新日期：1983-09-01 00:00:00

abstract：:Postsynaptic neuronal dendrites undergo functional and morphological changes in response to pathologically excessive synaptic activation. Although rapid formation of segmental focal swelling (varicosity) is the most prominent hallmark in such excitotoxic injury, little is known about the pathophysiological function of...

journal_title：Journal of cell science

authors： Ikegaya Y,Kim JA,Baba M,Iwatsubo T,Nishiyama N,Matsuki N

更新日期：2001-11-01 00:00:00

abstract：:An electron microscopic investigation of the events associated with meiosis in Lilium has revealed a number of changes in both the organellar population and the other cytoplasmic components. Ribosome numbers decrease significantly in early prophase and are later replenished in the tetrads, a process most likely involv...

journal_title：Journal of cell science

authors： Bird J,Porter EK,Dickinson HG

更新日期：1983-01-01 00:00:00

abstract：:Members of the ezrin-radixin-moesin (ERM) family of proteins are involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules that are implicated in cell adhesion, cell polarity and migration. Increas...

journal_title：Journal of cell science

authors： Clucas J,Valderrama F

更新日期：2014-01-15 00:00:00

abstract：:Murine mesenchymal stem cells are capable of differentiating in vitro into different lineages under stimulation with certain cytokines, growth factors and chemicals. However, the true capacity of these cells to contribute to different cell-types in vivo is still unclear, especially under minimal injury conditions. In ...

journal_title：Journal of cell science

authors： Anjos-Afonso F,Siapati EK,Bonnet D

更新日期：2004-11-01 00:00:00

abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...

journal_title：Journal of cell science

authors： Harper CV,Cummerson JA,White MR,Publicover SJ,Johnson PM

更新日期：2008-07-01 00:00:00

abstract：:Chromatin is no longer considered to be a static structural framework for packaging DNA within the nucleus but is instead believed to be an interactive component of DNA metabolism. The ordered assembly of chromatin produces a nucleoprotein template capable of epigenetically regulating the expression and maintenance of...

journal_title：Journal of cell science

authors： Ridgway P,Almouzni G

更新日期：2000-08-01 00:00:00

abstract：:Recently, several 3D images of kinesin-family motor domains interacting with microtubules have been obtained by analysis of electron microscope images of frozen hydrated complexes at much higher resolutions (9-12 A) than in previous reports (15-30 A). The high-resolution maps show a complex interaction interface betwe...

journal_title：Journal of cell science

authors： Amos LA,Hirose K

更新日期：2007-11-15 00:00:00

abstract：:Asymmetric cell division can produce daughter cells with different developmental fates and is often accompanied by a difference in cell size. A number of recent genetic and in vivo imaging studies in Drosophila and Caenorhabditis elegans have begun to elucidate the mechanisms underlying the rearrangements of the cytos...

journal_title：Journal of cell science

authors： Kaltschmidt JA,Brand AH

更新日期：2002-06-01 00:00:00

abstract：:Although the long-term effects of all-trans retinoic acid (RA) on neuronal growth and differentiation have been intensively studied, nothing is known about its effect on synaptic transmission. Here we show that RA rapidly and specifically enhances the spontaneous acetylcholine release at developing neuromuscular synap...

journal_title：Journal of cell science

authors： Liao YP,Ho SY,Liou JC

更新日期：2004-06-15 00:00:00

abstract：:Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein levels of NOS2, and synth...

journal_title：Journal of cell science

authors： Navarro-Lérida I,Portolés MT,Barrientos AA,Gavilanes F,Boscá L,Rodríguez-Crespo I

更新日期：2004-04-01 00:00:00

abstract：:Epithelial cell polarity is essential for animal development. The scaffold protein Bazooka (Baz/PAR-3) forms apical polarity landmarks to organize epithelial cells. However, it is unclear how Baz is recruited to the plasma membrane and how this is coupled with downstream effects. Baz contains an oligomerization domain...

journal_title：Journal of cell science

authors： McKinley RF,Yu CG,Harris TJ

更新日期：2012-03-01 00:00:00