Abstract：
:Biofilm formation, in which bacteria are embedded within an extracellular matrix, is the default form of microbial life in most natural and engineered habitats. In this work, atomistic molecular dynamics simulations were employed to examine the self-assembly of the polysaccharide Granulan to provide insight into the molecular interactions that lead to biofilm formation. Granulan is a major gel forming matrix component of granular microbial biofilms found in used-water treatment systems. Molecular dynamics simulations showed that Granulan forms an antiparallel double helix stabilized by complementary hydrogen bonds between the β-glucosamine of one strand and the N-acetyl-β-galactosamine-2-acetoamido-2-deoxy-α-galactopyranuronic pair of the other in both the presence and absence of Ca(2+). It is shown that Ca(2+) binds primarily to the carboxyl group of the terminal hexuronic acid of the sugar branch and that interactions between branches mediated by Ca(2+) suggest a possible mechanism for strengthening gels by facilitating interhelical bridging.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Seviour T,Malde AK,Kjelleberg S,Yuan Z,Mark AEdoi
10.1021/bm3005808subject
Has Abstractpub_date
2012-06-11 00:00:00pages
1965-72issue
6eissn
1525-7797issn
1526-4602journal_volume
13pub_type
杂志文章abstract：:The microstructure and the rheological properties of pure HM (high methoxyl) and LM (low methoxyl) pectin gels and of mixed HM/LM pectin gels have been investigated. Gel formation of either the HM or LM pectin, or both, was initiated in the mixed gels by varying the sucrose and Ca(2+) content. The microstructure was c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020044v
更新日期：2002-11-01 00:00:00
abstract：:For developing broader application of biobased polymers, graft copolymers and comb polymers having poly(lactic acid) (PLA) side chains have been synthesized by using a macromonomer technique. PLA macromonomers (MMm) having a methacryloyl polymerizable group with different PLA chain length with an average length m = 4,...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301212a
更新日期：2012-11-12 00:00:00
abstract：:The objective of these investigations was to test the hypothesis that a rapid cytoplasmic release profile from nanoparticles would potentiate the anticancer activity of cisplatin. Cisplatin-loaded nanoparticles with pH-responsive poly[2-(N,N-diethylamino)ethyl methacrylate] (PDEA) cores were synthesized from PDEA-bloc...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050902y
更新日期：2006-03-01 00:00:00
abstract：:Poly(L-lactide)-based microspheres having cationic or anionic surfaces were prepared using polydepsipeptide-block-poly(L-lactide)s as surfactants. Polydepsipeptide-block-poly(L-lactide)s having amino or carboxylic acid groups on their side chains were synthesized through anionic ring-opening polymerizations of L-lacti...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0200231
更新日期：2002-09-01 00:00:00
abstract：:There has been little study on the effect of composition or molecular weight on the biodegradation rate of photo-cross-linked biodegradable aliphatic polyesters though such information is important for tissue engineering scaffolds. We have synthesized a new series of photopolymerizable linear poly(epsilon-caprolactone...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060766c
更新日期：2007-02-01 00:00:00
abstract：:The effect of guluronate oligomers on the barrier properties of mucous matrices was investigated in terms of the mobility of nanoparticles in mucous matrices by fluorescence recovery after photobleaching (FRAP), cellular uptake of nanoparticles in mucus secreting cells (HT29-MTX), and mucin matrix architecture by scan...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500464b
更新日期：2014-06-09 00:00:00
abstract：:We report the preparation, characterization, and mechanical properties of DNA/poly(allylamine hydrochloride) (PAH) multilayer microcapsules. The DNA/PAH multilayers were first constructed on a planar support to examine their layer-by-layer buildup. Surface plasmon resonance spectroscopy (SPR) showed a nonlinear growth...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049254t
更新日期：2005-05-01 00:00:00
abstract：:Chemotherapy faces challenges, including poor aqueous solubility of the drugs, and cardiotoxicity. Micellar drug delivery systems (DDS) are used to encapsulate anticancer drugs for better therapeutic effects, however, with poor loading content. Herein, we synthesized a micellar DDS using γ-benzyloxy substituted poly(ε...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01742
更新日期：2020-04-13 00:00:00
abstract：:Poly(L-lactide) (PLLA) with various alkyl ester chain end groups were synthesized by ring-opening polymerization of L-lactide in the presence of zinc alkoxide as a catalyst. The structural effect of chain end groups on the rate of enzymatic and nonenzymatic degradations for amorphous films of PLLA were investigated at...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701259r
更新日期：2008-03-01 00:00:00
abstract：:A new α-amylase from Rhizomucor sp. (RA) was studied in detail due to its very efficient hydrolysis of raw starch granules at low temperature (32 °C). RA contains a starch binding domain (SBD) connected to the core amylase catalytic domain by a O-glycosylated linker. The mode of degradation of native maize starch gran...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100913z
更新日期：2011-01-10 00:00:00
abstract：:The intermolecular interactions of lignin with a hydrophilic polymer, poly(vinyl alcohol) (PVA), were studied using thermal analyses and FT-IR spectroscopy of a series of PVA/hardwood kraft lignin blend fibers prepared by thermal extrusion. Although two phases are observed in this blend system, some of the lignin was ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025727p
更新日期：2003-05-01 00:00:00
abstract：:In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers at ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025775+
更新日期：2003-07-01 00:00:00
abstract：:Double-network (DN) hydrogels are fabricated from poly(2-ethyl-2-oxazoline) (PEOXA)-peptide conjugates, which can be enzymatically crosslinked in the presence of Sortase A (SA), and physical networks of alginate (Alg), yielding matrices with improved mechanical properties with respect to the corresponding PEOXA and Al...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01266
更新日期：2019-12-09 00:00:00
abstract：:The formation of protein-polymer complexes was studied in an aqueous system using dynamic light scattering (DLS) and static light scattering (SLS) as the main experimental tools. Human serum albumin (HSA) was used as a protein and complexed with four representative water-soluble polymers: poly(N-isopropylacrylamide) (...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0341935
更新日期：2003-11-01 00:00:00
abstract：:The thermal conductive properties, including the thermal diffusivity and resultant thermal conductivity, of nonwoven nanocellulose sheets were investigated by separately measuring the thermal diffusivity of the sheets in the in-plane and thickness directions with a periodic heating method. The cross-sectional area (or...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00617
更新日期：2015-07-13 00:00:00
abstract：:Amphiphilic ABC triblock copolymers composed of monomethoxy-capped poly(ethylene glycol) (MPEG), poly(2-(dimethylamino)ethyl methacrylate) (DMA), and poly(2-(diethylamino)ethyl methacrylate) (DEA) have been synthesized by atom transfer radical polymerization (ATRP). These copolymers dissolve molecularly in acidic aque...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030026t
更新日期：2003-11-01 00:00:00
abstract：:An underwater bioadhesive generally comprises a multiprotein complex that provides a molecular basis for self-assembly. We report here a new class of self-assembling peptide inspired by a 20 kDa barnacle cement protein. Studies on the chemically synthesized 24-residue peptide have revealed that (1) it underwent irreve...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0612236
更新日期：2007-06-01 00:00:00
abstract：:Bone fractures constitute a major cause of morbidity and mortality especially in the elderly. Complications associated with osteoporosis drugs and the age of the patient slow bone turnover and render such fractures difficult to heal. Increasing the speed of fracture repair by administration of a fracture-targeted bone...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00777
更新日期：2015-10-12 00:00:00
abstract：:Core-shell structured nanoparticles (NPs) render the simultaneous coloading capacity of both hydrophobic and hydrophilic drugs and may eventually enhance therapeutic efficacy. In this study, we employed a facile squalenoylation technology to synthesize a new amphiphilic starch derivative from partially oxidized starch...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01430
更新日期：2020-12-21 00:00:00
abstract：:Water-soluble conjugated polymers (WCPs) composed of a hydrophobic polythiophene main chain with hydrophilic tertiary amine side-chains can directly self-assemble into sphere-like nano-objects in an aqueous solution due to phase separation between the hydrophilic and hydrophobic segments of the polymeric structure. Du...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01336
更新日期：2020-12-14 00:00:00
abstract：:G-quadruplex is a type of DNA secondary structure formed by specific guanine-rich sequences. Because of their enrichment in functional genomic regions and their biological significance, G-quadruplexes are recognized as significant drug targets for cancer and other diseases. Here, we tested the precipitation efficiency...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100862k
更新日期：2010-12-13 00:00:00
abstract：:Nanoconfined self-assemblies within aerosol nanoparticles and control of the secondary structures are shown here upon ionically complexing poly(L-lysine) (PLL) with dodecylbenzenesulfonic acid (DBSA) surfactant and using solvents chloroform, 1-propanol, or dimethylformamide. Different solvent volatilities and drying t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500704e
更新日期：2014-07-14 00:00:00
abstract：:Hemicellulose-based hydrogels were prepared by radical polymerization of 2-hydroxyethyl methacrylate or poly(ethylene glycol) dimethacrylate with oligomeric hydrosoluble hemicellulose modified with well-defined amounts of methacrylic functions. The polymerization reaction was carried out in water at 40 degrees C using...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049515z
更新日期：2005-03-01 00:00:00
abstract：:Peptide ligation is an indispensable step in the chemical synthesis of target peptides and proteins that are difficult to synthesize at once by a solid-phase synthesis. The ligation reaction is generally conducted with two peptide fragments at a high aqueous concentration to increase the reaction rate; however, this o...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01655
更新日期：2019-03-11 00:00:00
abstract：:This paper reports the rheological behavior of chitosan solutions that have been cross-linked with different amounts of genipin, at body temperature and physiological pH. The effect of the cross-linker loading on the rheological properties of hydrogels has been evaluated. The oscillatory time sweep method was used to ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700762w
更新日期：2007-12-01 00:00:00
abstract：:A multifunctional macromolecular thiol (TPVA) obtained by esterification of poly(vinyl alcohol) (PVA) with 3-mercaptopropionic acid was characterized by a combination of NMR, IR, transmission electron microscopy (TEM), and differential scanning calorimetry (DSC), and was used as a wheat gluten (WG) reactive modifier. ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060432n
更新日期：2006-10-01 00:00:00
abstract：:Strong signaling cascades derived from upregulation and overexpression of growth factors such as the EGF-family (epidermal growth factors) have been crucially related to cancer pathogenesis. Gene silencing techniques to modulate the expression of oncogenes and tumor suppresor genes are a strategy that shows great prom...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01066
更新日期：2018-10-08 00:00:00
abstract：:Cellulose is the most familiar and most abundant strong biopolymer, but the reasons for its outstanding mechanical performance are not well understood. Each glucose unit in a cellulose chain is joined to the next by a covalent C-O-C linkage flanked by two hydrogen bonds. This geometry suggests some form of cooperativi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401616n
更新日期：2014-03-10 00:00:00
abstract：:Targeted delivery of antigens to immune cells using micro/nanocarriers may serve as a therapeutic application for vaccination. However, synthetic carriers have potential drawbacks including cytotoxicity, low encapsulation efficiency of antigen, and lack of a morphological design, which limit the translation of the del...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01775
更新日期：2020-06-08 00:00:00
abstract：:Amphiphilic glycopolymers, polylactide-grafted dextran copolymers (Dex-g-PLA), were synthesized with a well-controlled architecture obtained through a three-step procedure: partial silylation of the dextran hydroxyl groups, ring-opening polymerization of D,L-lactide initiated from remaining hydroxyl groups, silylether...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701101n
更新日期：2008-03-01 00:00:00
