Abstract:
:Nanoconfined self-assemblies within aerosol nanoparticles and control of the secondary structures are shown here upon ionically complexing poly(L-lysine) (PLL) with dodecylbenzenesulfonic acid (DBSA) surfactant and using solvents chloroform, 1-propanol, or dimethylformamide. Different solvent volatilities and drying temperatures allowed tuning the kinetics of morphology formation. The supramolecular self-assembly and morphology were studied using cryo-TEM and SEM, and the secondary structures, using FT-IR. Highly volatile chloroform led to the major fraction of α-helical conformation of PLL(DBSA), whereas less volatile solvents or higher drying temperatures led to the increasing fraction of β-sheets. Added drugs budesonide and ketoprofen prevented β-sheet formation and studied PLL(DBSA)-drug nanoparticles were in the α-helical conformation. Preliminary studies showed that ketoprofen released with a slower rate than budesonide which was hypothesized to result from different localization of drugs within the PLL(DBSA) nanoparticles. These results instruct to prepare polypeptide aerosol nanoparticles with internal self-assembled structures and to control the secondary structures by aerosol solvent annealing, which we foresee to be useful, e.g., toward controlling the release of poorly soluble drug molecules.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Rahikkala A,Junnila S,Vartiainen V,Ruokolainen J,Ikkala O,Kauppinen E,Raula Jdoi
10.1021/bm500704esubject
Has Abstractpub_date
2014-07-14 00:00:00pages
2607-15issue
7eissn
1525-7797issn
1526-4602journal_volume
15pub_type
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