Abstract:
:Artemisinin, the active ingredient of the Chinese medicinal herb Artemisia annua L., and its derivatives (ARTs) are currently widely used as anti-malarial drugs around the world. In this study, we found that dihydroartemisinin (DHA), one of the main active metabolites of ARTs, inhibited the proliferation of human hepatocarcinoma BEL-7402 cells in a concentration-dependent manner. To interpret the mechanisms involved, an analysis of the mitochondrial proteome was performed employing two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Seven mitochondrial proteins including fumarate hydratase, 60 kDa heat shock protein, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, two subunits of ATP synthase and NADPH:adrenodoxin oxidoreductase were identified to be differentially expressed between the control and DHA-treated groups. Our results indicate that the imbalance of energy metabolism induced by DHA may contribute, at least in part, to its anti-cancer potential in BEL-7402 cells.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Lu JJ,Yang Z,Lu DZ,Wo XD,Shi JJ,Lin TQ,Wang MM,Li Y,Tang LHdoi
10.3892/mmr.2012.906subject
Has Abstractpub_date
2012-08-01 00:00:00pages
429-33issue
2eissn
1791-2997issn
1791-3004journal_volume
6pub_type
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