Abstract:
:Metastasis is the most lethal stage of cancer progression. The present study aimed to investigate the underlying molecular mechanisms of melanoma metastasis using bioinformatics. Using the microarray dataset GSE8401 from the Gene Expression Omnibus database, which included 52 biopsy specimens from patients with melanoma metastasis and 31 biopsy specimens from patients with primary melanoma, differentially expressed genes (DEGs) were identified, subsequent to data preprocessing with the affy package, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. A protein‑protein interaction (PPI) network was constructed. Mutated genes were analyzed with 80 mutated cases with melanoma from The Cancer Genome Atlas. The overall survival of key candidate DEGs, which were within a filtering of degree >30 criteria in the PPI network and involved three or more KEGG signaling pathways, and genes with a high mutation frequency were delineated. The expression analysis of key candidate DEGs, mutant genes and their associated genes were performed on UALCAN. Of the 1,187 DEGs obtained, 505 were upregulated and 682 were downregulated. 'Extracellular exosome' processes, the 'amoebiasis' pathway, the 'ECM‑receptor interaction' pathway and the 'focal adhesion' signaling pathway were significantly enriched and identified as important processes or signaling pathways. The overall survival analysis of phosphoinositide‑3‑kinase regulator subunit 3 (PIK3R3), centromere protein M (CENPM), aurora kinase A (AURKA), laminin subunit α 1 (LAMA1), proliferating cell nuclear antigen (PCNA), adenylate cyclase 1 (ADCY1), BUB1 mitotic checkpoint serine/threonine kinase (BUB1), NDC80 kinetochore complex component (NDC80) and protein kinase C α (PRKCA) in DEGs was statistically significant. Mutation gene analysis identified that BRCA1‑associated protein 1 (BAP1) had a higher mutation frequency and survival analysis, and its associated genes in the BAP1‑associated PPI network, including ASXL transcriptional regulator 1 (ASXL1), proteasome 26S subunit, non‑ATPase 3 (PSMD3), proteasome 26S subunit, non ATPase 11 (PSMD11) and ubiquitin C (UBC), were statistically significantly associated with the overall survival of patients with melanoma. The expression levels of PRKCA, BUB1, BAP1 and ASXL1 were significantly different between primary melanoma and metastatic melanoma. Based on the present study, 'extracellular exosome' processes, 'amoebiasis' pathways, 'ECM‑receptor interaction' pathways and 'focal adhesion' signaling pathways may be important in the formation of metastases from melanoma. The involved genes, including PIK3R3, CENPM, AURKA, LAMA1, PCNA, ADCY1, BUB1, NDC80 and PRKCA, and mutation associated genes, including BAP1, ASXL1, PSMD3, PSMD11 and UBC, may serve important roles in metastases of melanoma.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Chen J,Wu F,Shi Y,Yang D,Xu M,Lai Y,Liu Ydoi
10.3892/mmr.2019.10314subject
Has Abstractpub_date
2019-08-01 00:00:00pages
903-914issue
2eissn
1791-2997issn
1791-3004journal_volume
20pub_type
杂志文章abstract::As the resident macrophages of the brain's innate immune system, microglial cells are key modulators in the neurodegenerative disease Alzheimer's disease (AD). In particular, the activation and accumulation of microglial cells around amyloid plaques is considered to result in chronic neuroinflammation. Although the pa...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8524
更新日期:2018-04-01 00:00:00
abstract::In order to gain greater understanding of the mechanisms underlying the effect of epigallocatechin-3-gallate (EGCG) on DNA methylation and its chemopreventative action in oral squamous cell carcinoma (OSCC), a genome‑wide methylation and mRNA expression screen was performed in the CAL‑27 cell line with and without EGC...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4118
更新日期:2015-10-01 00:00:00
abstract::Ribavirin (RBV)-induced anemia is a serious side effect of pegylated interferon (PEG-IFN) plus RBV therapy which is the standard care most effective for hepatitis C virus (HCV) infection. In the present study, we investigated the association of inosine triphosphate pyrophosphatase (ITPA) genotypes with RBV-induced hem...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2011.659
更新日期:2012-02-01 00:00:00
abstract::Skeletal myogenesis is a highly ordered and complex biological process that is mediated by numerous regulatory factors. In previous studies, it has been demonstrated that microRNAs (miRs) and long non‑coding RNAs (lncRNAs) serve key roles in skeletal myogenesis. The present study showed that the expression levels of m...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11475
更新日期:2020-11-01 00:00:00
abstract::The aim of the present study was to investigate the potential role and regulatory mechanism of long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 (FTH1P3) in glioma development. The expression of FTH1P3 in low‑ and high‑grade glioma tissues was investigated using reverse transcription‑quantitative polymeras...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9491
更新日期:2018-11-01 00:00:00
abstract::Oxidized low-density lipoprotein (Ox-LDL), as a strong oxidant, results in renal injury through multiple mechanisms. The aim of the present study was to determine the injury effects of Ox‑LDL and the potential protective effects of the antioxidant reagent probucol on epithelial‑mesenchymal transition (EMT) in human re...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7935
更新日期:2018-01-01 00:00:00
abstract::The aim of this study was to investigate the protective effect of erythropoietin (EPO) against acute myocardial injury and its underlying mechanisms. Mice (n=146) were randomly divided in a double‑blind manner into four groups, sham, Rocephin, EPO and sepsis, and mortality was observed on the seventh day after cecal l...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.3155
更新日期:2015-05-01 00:00:00
abstract::Despite advances in the treatment of T‑cell acute lymphoblastic leukemia (T‑ALL), the outcome of T‑ALL treatment remains unsatisfactory, therefore, more effective treatment is urgently required. The present study examined the cytotoxicities of bortezomib in combination with daunorubicin against human Jurkat and Molt‑4...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6554
更新日期:2017-07-01 00:00:00
abstract::Minoxidil and finasteride have been approved to treat hair loss by the Food and Drug Administration. However, the further elucidation of treatments for hair loss, including those using Chinese herbal medicine, remains important clinically. BeauTop (BT) is a health food supplement which contains Ginseng radix, Astragal...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8917
更新日期:2018-06-01 00:00:00
abstract::Multipotent differentiation ability, low expression of co‑stimulatory molecules and immunosuppressive effects render mesenchymal stem cells (MSCs) the best candidate for use in cell‑based therapy. At present, MSCs are widely used in clinical trials, which have, however, failed to demonstrate any long‑term therapeutic ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4276
更新日期:2015-11-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a life‑threatening disease induced by the excessive proliferation and reduced apoptosis of pulmonary artery smooth muscle cells (PASMCs). Formononetin (FMN) is a natural isoflavone with numerous cardioprotective properties, which can inhibit the proliferation and induce the apo...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10781
更新日期:2019-12-01 00:00:00
abstract::Purple sweet potato color (PSPC) is a natural anthocyanin pigment that is derived from purple sweet potato storage roots. PSPC possesses a variety of biological activities, including antioxidant, anti‑inflammatory and neuroprotective effects; however, the detailed effects of PSPC on high‑fat diet (HFD)‑induced neuroin...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8440
更新日期:2018-03-01 00:00:00
abstract::Multidrug resistance (MDR) is a major impediment to cancer therapy. MG‑132 has been identified to be effective against MDR in several types of cancer. However, the mechanism of MG‑132 in head and neck squamous cell carcinomas remains unknown. Based on our previous study, the present detected P‑gp and P‑gp expression i...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9138
更新日期:2018-08-01 00:00:00
abstract::Irinotecan (CTP-11) is a topoisomerase I inhibitor used in the treatment of colorectal cancer and non-small cell lung cancer (NSCLC). Despite an initial response to therapy, resistance to irinotecan reduces its efficacy. We isolated irinotecan-resistant human NSCLC A549 cells, termed A549/CTP-11R cells. A549/CTP-11R c...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2010.366
更新日期:2010-11-01 00:00:00
abstract::The present study aimed to investigate the effects of sufentanil on sepsis-induced acute lung injury (ALI), and identify the potential molecular mechanisms underlying its effect. In order to achieve this, a rat sepsis model was established. Following treatment with sufentanil, the lung wet/dry (W/D) weight ratio was c...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11526
更新日期:2020-11-01 00:00:00
abstract::Pregnancy complications are associated with abnormal cytotrophoblast differentiation and invasion. Hydrogen peroxide (H2O2) is an important mediator of oxidative ischemia/reperfusion stress in the placenta. Lycium barbarum polysaccharides (LBP) have been demonstrated to counteract oxidative free radicals. The effects ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9274
更新日期:2018-09-01 00:00:00
abstract::The aim of this study was to investigate the correlation between brain-derived neurotrophic factor (BDNF) expression and cognitive impairment in post‑stroke depression (PSD) rats and to explore the mechanism(s) involved in the process of cognitive impairment. A rat model of focal ce...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.1009
更新日期:2012-10-01 00:00:00
abstract::Inflammatory responses are vital in lung injury diseases, particularly acute respiratory distress syndrome (ARDS). Recombinant human brain natriuretic peptide (rhBNP) has been shown to exhibit anti‑inflammatory effects in vivo in our previous studies. The present study aimed to investigate the mechanisms underlying th...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5400
更新日期:2016-08-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a group of inherited diseases that primarily affect light‑sensitive rods and cones in the retina. Rhodopsin mutations, including the T17M mutation, are associated with the autosomal dominant form of retinitis pigmentosa (ADRP) and have been linked to abnormal protein folding. However, the ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.1987
更新日期:2014-05-01 00:00:00
abstract::Transforming growth factor (TGF)‑β1 has a key role in the regulation of fibrosis and organ dysfunction. During the pathogenesis and progression of vital organ fibrosis, the microRNA (miR)‑29 family is irregularly downregulated and exogenous supplementation of miR‑29b has a strong anti‑fibrotic capacity. However, wheth...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5062
更新日期:2016-05-01 00:00:00
abstract::Patients with respiratory overlap syndrome (OS), defined as concomitant chronic obstructive pulmonary disease and obstructive sleep apnea syndrome, may exhibit an increased blood concentration of ingested drugs. This poor elimination of drugs is primarily attributed to downregulated gene expression of the drug‑metabol...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6642
更新日期:2017-07-01 00:00:00
abstract::Cisplatin-induced heart damage is associated with enzymes and protein alterations. The purpose of this study was to investigate cisplatin-induced alterations in cardiac endothelin (ET)-1 and type III collagen following administration of cisplatin. Male Wistar rats were randomly divided into two groups: a control and a...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2011.706
更新日期:2012-03-01 00:00:00
abstract::Resveratrol is a natural compound present in red grapes and red wine, and is often consumed in the human diet. The chemopreventive and chemotherapeutic potential of resveratrol in hepatocellular carcinoma (HCC), one of the most common types of liver cancer, has received much attention in recent years. However, the sid...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.1077
更新日期:2012-12-01 00:00:00
abstract::The present study aimed to identify potentially important biomarkers associated with polycystic ovary syndrome (PCOS) by integrating DNA methylation with transcriptome profiling. The transcription (E‑MTAB‑3768) and methylation (E‑MTAB‑3777) datasets were retrieved from ArrayExpress. Paired transcription and methylatio...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11005
更新日期:2020-05-01 00:00:00
abstract::To investigate the association between apolipo-protein E (APOE) polymorphisms and insulin resistance and Traditional Chinese Medicine (TCM) syndromes in type 2 diabetes mellitus (T2DM) with macroangiopathy, 60 patients with T2DM macroangiopathy were enrolled and divided into three groups: dryness-heat due to deficienc...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2011.546
更新日期:2011-11-01 00:00:00
abstract::GLI family zinc finger 3 (Gli3), as the upstream transcriptional activator of hedgehog signaling, has previously been demonstrated to participate in the process of liver fibrosis. The present study aimed to investigate the potential functions of microRNA (miR)‑200a and Gli3 in the progression of liver fibrosis. The ex...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.10997
更新日期:2020-04-01 00:00:00
abstract::Methyl‑CpG‑binding protein 2 (MeCP2) is a transcriptional repressor that has been implicated in tumor onset and progression. Compared with normal and other tumorous tissue, MeCP2 is highly expressed in well‑differentiated adenocarcinoma and mucinous adenocarcinoma tissues, particularly at the invasion site of colorect...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4612
更新日期:2016-01-01 00:00:00
abstract::Deoxyribonuclease I (DNase I) is an endonuclease responsible for the destruction of chromatin during apoptosis. However, its role in diabetes remains unclear. The aim of the current study was to investigate the role of DNase I combined with high glucose levels in β‑cell apoptosis. Human samples were collected and the ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5102
更新日期:2016-06-01 00:00:00
abstract::Chemokines are known to have anti-tumor effects due to their chemoattractant properties, which stimulate the accumulation of infiltrating immune cells in tumors. CCL22 (macrophage-derived chemokine, MDC) attracts killer T?cells, helper T cells and antigen-presenting cells expressing the CCL22 receptor, CCR4. Thus, CCL...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr_00000171
更新日期:2009-09-01 00:00:00
abstract::The zinc-fingers and homeoboxes protein 1 (ZHX1) consists of 873 amino acid residues, is localized in the cell nucleus and appears to act as a transcriptional repressor. Previous studies have shown that ZHX1 interacts with nuclear factor Y subunit α (NF-YA), DNA methyltransferases (DNMT) 3B and ZHX2, all of which are ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1700
更新日期:2013-11-01 00:00:00