当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > A more sensitive platform for the detection of low-abundance BRAF(V⁶⁰⁰E) mutations.

A more sensitive platform for the detection of low-abundance BRAF(V⁶⁰⁰E) mutations.

Abstract：

:Identifying low-abundance mutations is important for the therapy and diagnose of cancer. Since the potential for tumor heterogeneity, the efficient detection of cancer-relevant mutations largely depends on the sensitivity of the methods employed. To confirm whether the mutation detection platforms affect the perceived prevalence of the BRAF(V600E) and its correlation with clinicopathologic features in papillary thyroid carcinomas (PTC), we compared Sanger Sequencing (SS), Pyrosequencing (PS), and a newly built allele-specific real-time PCR (AS-qPCR) apparatus for the detection of BRAF(V600E) in a Chinese cohort of conventional variant PTC. Accurate plasmid standards were built to assess the limit of detection of the three platforms. In this research, AS-qPCR has been found both the most sensitive and reliable at detecting mutation. The mutations detected by AS-qPCR which were not detected by SS or PS due to low abundance were confirmed by mutation enrichment platform COLD-PCR followed by SS. When analyzed by AS-qPCR, BRAF(V600E) was associated with a more aggressive phenotype. Our results indicate that the reported prevalence of the BRAF(V600E) mutations in PTC has been underestimated and more sensitive methods such as AS-qPCR should be applied in clinical settings.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Jiang W,Wang W,Fu F,Teng X,Wang H,Wang H,Teng L

doi

10.1007/s11010-012-1282-2

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

49-58

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

366

pub_type

杂志文章

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